Restriction map for
pFN21ASDA0666
(Circular) (Six-base) MAPSORT of: pFN21ASDA0666.seq Check: 4468 from: 1 to: 7963
pFN21ASDA0666 7963 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3532 3532
Size: 7963
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5773 278
Size: 53 83 186 936 4237 2468
Fragments arranged by size:
4237 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5306 1937
Size: 3369 4594
Acc65I G'GTAC_C
Cuts at: 5284 5284
Size: 7963
AclI AA'CG_TT
Cuts at: 1563 6090 6463 1563
Size: 4527 373 3063
Fragments arranged by size:
4527 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 3836 4816 6026 7410 7678 1632
Size: 488 1716 980 1210 1384 268 1917
Fragments arranged by size:
1917 1716 1384 1210 980 488 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7766
AflIII A'CryG_T
Cuts at: 1203 4200 6862 7839 1203
Size: 2997 2662 977 1327
Fragments arranged by size:
2997 2662 1327 977
AgeI A'CCGG_T
Cuts at: 6794 6794
Size: 7963
AhdI GACnn_n'nnGTC
Cuts at: 6692 6692
Size: 7963
AleI CACnn'nnGTG
Cuts at: 1166 4610 1166
Size: 3444 4519
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7963
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 3995 4027 2174
Size: 32 1789 32 6110
Fragments arranged by size:
6110 1789 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2656 3418 4462 5706 7278 2656
Size: 762 1044 1244 1572 3341
Fragments arranged by size:
3341 1572 1244 1044 762
ApaI G_GGCC'C
Cuts at: 3639 3639
Size: 7963
ApaLI G'TGCA_C
Cuts at: 6020 7176 6020
Size: 1156 6807
ApoI r'AATT_y
Cuts at: 1493 2333 2942 2958 2995 3056 3936 4220 5272
Size: 840 609 16 37 61 880 284 1052
Cuts at: 5272 5498 5650 7759 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 880 840 609 284 226
152 61 37 16
AseI AT'TA_AT
Cuts at: 160 1032 2637 2801 2877 4323 6517 160
Size: 872 1605 164 76 1446 2194 1606
Fragments arranged by size:
2194 1606 1605 1446 872 164 76
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7963
AvaI C'yCGr_G
Cuts at: 1870 1943 2723 3878 5288 1870
Size: 73 780 1155 1410 4545
Fragments arranged by size:
4545 1410 1155 780 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3912 3945 3912
Size: 33 7930
BamHI G'GATC_C
Cuts at: 5293 5660 5293
Size: 367 7596
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 4445 5284 6739 618
Size: 325 217 627 918 1740 839 1455 1842
Fragments arranged by size:
1842 1740 1455 918 839 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2611 3576 3639 4102 5035 5282 1391
Size: 1220 965 63 463 933 247 4072
Fragments arranged by size:
4072 1220 965 933 463 247 63
BbeI G_GCGC'C
Cuts at: 2709 2709
Size: 7963
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3922 3957 4857 928
Size: 971 2023 35 900 4034
Fragments arranged by size:
4034 2023 971 900 35
BbvCI CC'TCA_GC
Cuts at: 3834 3834
Size: 7963
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6154 6188 1903
Size: 34 4217 34 3678
Fragments arranged by size:
4217 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2513 5855 7065 2513
Size: 3342 1210 3411
Fragments arranged by size:
3411 3342 1210
BclI T'GATC_A
Cuts at: 1548 2086 2344 4951 1548
Size: 538 258 2607 4560
Fragments arranged by size:
4560 2607 538 258
BfrBI ATG'CAT
Cuts at: 4517 4517
Size: 7963
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6574 136
Size: 107 122 71 631 605 4902 1525
Fragments arranged by size:
4902 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3943 4186 7958 3943
Size: 243 3772 3948
Fragments arranged by size:
3948 3772 243
BlpI GC'TnA_GC
Cuts at: 2888 3575 5046 5380 2888
Size: 687 1471 334 5471
Fragments arranged by size:
5471 1471 687 334
Bme1580I G_kGCm'C
Cuts at: 1790 3639 4450 6024 7180 1790
Size: 1849 811 1574 1156 2573
Fragments arranged by size:
2573 1849 1574 1156 811
BmgBI CAC'GTC
Cuts at: 1318 2614 1318
Size: 1296 6667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2835 4623 5694
Size: 416 53 83 399 24 1418 1788 1071
Cuts at: 5694 6652 442
Size: 958 1753
Fragments arranged by size:
1788 1753 1418 1071 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3642 3717 4484 6623 1239
Size: 2403 75 767 2139 2579
Fragments arranged by size:
2579 2403 2139 767 75
Bpu10I CC'TnA_GC
Cuts at: 3834 5146 3834
Size: 1312 6651
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 2837 3438 4436 4653 4738 6088 6953 7251
Size: 254 601 998 217 85 1350 865 298
Cuts at: 7251 2583
Size: 3295
Fragments arranged by size:
3295 1350 998 865 601 298 254 217
85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 5227 6626 882
Size: 617 1029 2699 1399 2219
Fragments arranged by size:
2699 2219 1399 1029 617
BsaAI yAC'GTr
Cuts at: 493 1204 2103 5260 5754 493
Size: 711 899 3157 494 2702
Fragments arranged by size:
3157 2702 899 711 494
BsaBI GATnn'nnATC
Cuts at: 3260 7957 3260
Size: 4697 3266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2706 5770 6152 6787
Size: 53 83 186 936 1173 3064 382 635
Cuts at: 6787 275
Size: 1451
Fragments arranged by size:
3064 1451 1173 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6396 6794 7068 7215 7626 6396
Size: 398 274 147 411 6733
Fragments arranged by size:
6733 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3275 3305 3995 4025 1178
Size: 30 165 30 1872 30 690 30 5116
Fragments arranged by size:
5116 1872 690 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 3217 3735 3738 3753 1186
Size: 1003 1028 518 3 15 5396
Fragments arranged by size:
5396 1028 1003 518 15 3
BseYI C'CCAG_C
Cuts at: 7166 7662 7166
Size: 496 7467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 2919 3158 1163
Size: 573 1005 178 239 5968
Fragments arranged by size:
5968 1005 573 239 178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5413 6174 7202 1540
Size: 275 186 3412 761 1028 2301
Fragments arranged by size:
3412 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2663 2889 3399 3576 3628 3799 4102 5035 6024
Size: 226 510 177 52 171 303 933 989
Cuts at: 6024 6109 7180 2663
Size: 85 1071 3446
Fragments arranged by size:
3446 1071 989 933 510 303 226 177
171 85 52
BsiWI C'GTAC_G
Cuts at: 3328 3328
Size: 7963
BsmI GAATG_Cn'
Cuts at: 3828 4519 5474 5567 3828
Size: 691 955 93 6224
Fragments arranged by size:
6224 955 691 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2611 2663 2889 3399 3576 3628 3639
Size: 399 821 52 226 510 177 52 11
Cuts at: 3639 3799 4102 4450 5035 5282 6024 6109 7180
Size: 160 303 348 585 247 742 85 1071
Cuts at: 7180 1391
Size: 2174
Fragments arranged by size:
2174 1071 821 742 585 510 399 348
303 247 226 177 160 85 52 52
11
BspEI T'CCGG_A
Cuts at: 7626 7626
Size: 7963
BspHI T'CATG_A
Cuts at: 5852 7582 5852
Size: 1730 6233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3532 5318 844
Size: 2688 1786 3489
Fragments arranged by size:
3489 2688 1786
BsrBI CCG'CTC
Cuts at: 1394 1823 5850 1394
Size: 429 4027 3507
Fragments arranged by size:
4027 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2153 6458 6632 65
Size: 1183 905 4305 174 1396
Fragments arranged by size:
4305 1396 1183 905 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6607 6794 1700
Size: 110 4797 187 2869
Fragments arranged by size:
4797 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7963
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7963
BssSI C'ACGA_G
Cuts at: 6023 7035 6023
Size: 1012 6951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2752 4342 794
Size: 1958 1590 4415
Fragments arranged by size:
4415 1958 1590
BstBI TT'CG_AA
Cuts at: 2007 6767 2007
Size: 4760 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7963
BstXI CCAn_nnnn'nTGG
Cuts at: 3301 3301
Size: 7963
BstYI r'GATC_y
Cuts at: 1972 3659 3943 4186 5293 5660 6045 6062 7514
Size: 1687 284 243 1107 367 385 17 1452
Cuts at: 7514 7600 7612 7958 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1687 1452 1107 385 367 346 284
243 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3769 3769
Size: 7963
BtgI C'CryG_G
Cuts at: 513 1065 2022 2142 2358 2523 513
Size: 552 957 120 216 165 5953
Fragments arranged by size:
5953 957 552 216 165 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7224
Fragments arranged by size:
7224 683 56
BtsI GCAGTG_nn'
Cuts at: 2889 3178 3368 4896 5479 6273 6293 2889
Size: 289 190 1528 583 794 20 4559
Fragments arranged by size:
4559 1528 794 583 289 190 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3187 3222 529
Size: 35 2623 35 5270
Fragments arranged by size:
5270 2623 35 35
DraI TTT'AAA
Cuts at: 2433 5267 2433
Size: 2834 5129
DraIII CAC_nnn'GTG
Cuts at: 3143 3143
Size: 7963
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2542 6970 809
Size: 507 1226 4428 1802
Fragments arranged by size:
4428 1802 1226 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 4284 5410
Size: 54 1425 50 275 18 855 1599 1126
Cuts at: 5410 6299 7928 8
Size: 889 1629 43
Fragments arranged by size:
1629 1599 1425 1126 889 855 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5410 1537
Size: 275 3598 4090
Fragments arranged by size:
4090 3598 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3122 3459 4422 4902 5893 1353
Size: 1769 337 963 480 991 3423
Fragments arranged by size:
3423 1769 991 963 480 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3563 6550 6924 7070 387
Size: 1064 138 1974 2987 374 146 1280
Fragments arranged by size:
2987 1974 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3574 4100 5033 3574
Size: 526 933 6504
Fragments arranged by size:
6504 933 526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3642 3717 3836 4484 4816 6026
Size: 393 488 1522 75 119 648 332 1210
Cuts at: 6026 6623 7410 7678 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1522 1210 787 648 597 488 393
332 268 119 75
EcoNI CCTnn'n_nnAGG
Cuts at: 3693 3753 3768 4253 3693
Size: 60 15 485 7403
Fragments arranged by size:
7403 485 60 15
EcoO109I rG'GnC_Cy
Cuts at: 2784 3283 3635 3636 3758 2784
Size: 499 352 1 122 6989
Fragments arranged by size:
6989 499 352 122 1
EcoRI G'AATT_C
Cuts at: 2333 2958 5272 5650 2333
Size: 625 2314 378 4646
Fragments arranged by size:
4646 2314 625 378
EcoRV GAT'ATC
Cuts at: 4533 4533
Size: 7963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3518 3518
Size: 7963
FspI TGC'GCA
Cuts at: 6469 6469
Size: 7963
HaeII r_GCGC'y
Cuts at: 1678 2709 7110 1678
Size: 1031 4401 2531
Fragments arranged by size:
4401 2531 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2480 2512 3275 3307 4075
Size: 32 135 32 908 32 763 32 768
Cuts at: 4075 4107 4547 4579 6604 6636 6678 6710 1373
Size: 32 440 32 2025 32 42 32 2626
Fragments arranged by size:
2626 2025 908 768 763 440 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5307 5553 669
Size: 988 281 3369 246 3079
Fragments arranged by size:
3369 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3147 748
Size: 2399 5564
HpaI GTT'AAC
Cuts at: 5553 5553
Size: 7963
KasI G'GCGC_C
Cuts at: 2705 2705
Size: 7963
KpnI G_GTAC'C
Cuts at: 5288 5288
Size: 7963
MfeI C'AATT_G
Cuts at: 5562 5562
Size: 7963
MluI A'CGCG_T
Cuts at: 7839 7839
Size: 7963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4073 4185 7077 7261 1305
Size: 2768 112 2892 184 2007
Fragments arranged by size:
2892 2768 2007 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4286 10
Size: 54 1425 343 2454 3687
Fragments arranged by size:
3687 2454 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4610 5921 6280 6439 518
Size: 648 3444 1311 359 159 2042
Fragments arranged by size:
3444 2042 1311 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2258 5379 6056 7204 7449 1606
Size: 652 3121 677 1148 245 2120
Fragments arranged by size:
3121 2120 1148 677 652 245
NarI GG'CG_CC
Cuts at: 2706 2706
Size: 7963
NcoI C'CATG_G
Cuts at: 513 1065 2022 2142 2358 2523 513
Size: 552 957 120 216 165 5953
Fragments arranged by size:
5953 957 552 216 165 120
NdeI CA'TA_TG
Cuts at: 387 4519 387
Size: 4132 3831
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7963
NotI GC'GGCC_GC
Cuts at: 5410 5410
Size: 7963
NsiI A_TGCA'T
Cuts at: 4519 4519
Size: 7963
NspI r_CATG'y
Cuts at: 1132 1229 4204 5321 6866 1132
Size: 97 2975 1117 1545 2229
Fragments arranged by size:
2975 2229 1545 1117 97
PacI TTA_AT'TAA
Cuts at: 2805 2805
Size: 7963
PasI CC'CwG_GG
Cuts at: 1348 3755 1348
Size: 2407 5556
PciI A'CATG_T
Cuts at: 4200 6862 4200
Size: 2662 5301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3791 1108
Size: 2683 5280
PmeI GTTT'AAAC
Cuts at: 5267 5267
Size: 7963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2202 2234 3995 4027 6032 6064 7545 7577 2202
Size: 32 1761 32 2005 32 1481 32 2588
Fragments arranged by size:
2588 2005 1761 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2784 3283 2784
Size: 499 7464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7963
PsiI TTA'TAA
Cuts at: 5533 5533
Size: 7963
PspOMI G'GGCC_C
Cuts at: 3635 3635
Size: 7963
PstI C_TGCA'G
Cuts at: 830 2748 5315 830
Size: 1918 2567 3478
Fragments arranged by size:
3478 2567 1918
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7963
PvuII CAG'CTG
Cuts at: 2258 2258
Size: 7963
SacI G_AGCT'C
Cuts at: 3576 4102 5035 3576
Size: 526 933 6504
Fragments arranged by size:
6504 933 526
SalI G'TCGA_C
Cuts at: 1936 5305 1936
Size: 3369 4594
SbfI CC_TGCA'GG
Cuts at: 5315 5315
Size: 7963
ScaI AGT'ACT
Cuts at: 3224 6211 7923 3224
Size: 2987 1712 3264
Fragments arranged by size:
3264 2987 1712
SexAI A'CCwGG_T
Cuts at: 3694 3694
Size: 7963
SfcI C'TryA_G
Cuts at: 826 1045 2281 2421 2744 5311 6446 7127 7318
Size: 219 1236 140 323 2567 1135 681 191
Cuts at: 7318 826
Size: 1471
Fragments arranged by size:
2567 1471 1236 1135 681 323 219 191
140
SfoI GGC'GCC
Cuts at: 2707 2707
Size: 7963
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7963
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7963
SmaI CCC'GGG
Cuts at: 1872 2725 5290 1872
Size: 853 2565 4545
Fragments arranged by size:
4545 2565 853
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 2852 3453 4451 4668 4717
Size: 197 926 655 254 601 998 217 49
Cuts at: 4717 6067 6968 7230 820
Size: 1350 901 262 1553
Fragments arranged by size:
1553 1350 998 926 901 655 601 262
254 217 197 49
SnaBI TAC'GTA
Cuts at: 493 5260 5754 493
Size: 4767 494 2702
Fragments arranged by size:
4767 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7963
SphI G_CATG'C
Cuts at: 1132 5321 1132
Size: 4189 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5887 5
Size: 47 3264 2571 2081
Fragments arranged by size:
3264 2571 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7963
StyI C'CwwG_G
Cuts at: 513 1065 2022 2142 2358 2523 2893 4194 4287
Size: 552 957 120 216 165 370 1301 93
Cuts at: 4287 5402 513
Size: 1115 3074
Fragments arranged by size:
3074 1301 1115 957 552 370 216 165
120 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 3923 5990 6007 6160 6345 6790
Size: 1255 526 1817 2067 17 153 185 445
Cuts at: 6790 325
Size: 1498
Fragments arranged by size:
2067 1817 1498 1255 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 4353 6209
Size: 274 80 33 51 1443 1245 1131 1856
Cuts at: 6209 7921 96
Size: 1712 138
Fragments arranged by size:
1856 1712 1443 1245 1131 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7923 7955 7923
Size: 32 7931
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7963
XbaI T'CTAG_A
Cuts at: 3940 5299 3940
Size: 1359 6604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 4201 2167
Size: 2034 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7963
XmaI C'CCGG_G
Cuts at: 1870 2723 5288 1870
Size: 853 2565 4545
Fragments arranged by size:
4545 2565 853
XmnI GAAnn'nnTTC
Cuts at: 3078 3857 4410 4854 6092 3078
Size: 779 553 444 1238 4949
Fragments arranged by size:
4949 1238 779 553 444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5771 276
Size: 53 83 186 936 4237 2468
Fragments arranged by size:
4237 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BplI BsmBI BstZ17I FseI FspAI
NaeI NgoMIV NruI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SapI SfiI SrfI SwaI