Restriction map for
pFN21ASDA0665
(Circular) (Six-base) MAPSORT of: pFN21ASDA0665.seq Check: 94 from: 1 to: 6997
pFN21ASDA0665 6997 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2725 3924 2725
Size: 1199 5798
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2502 4807 278
Size: 53 83 186 936 966 2305 2468
Fragments arranged by size:
2468 2305 966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2496 2721 4340 1937
Size: 559 225 1619 4594
Fragments arranged by size:
4594 1619 559 225
Acc65I G'GTAC_C
Cuts at: 3324 4318 3324
Size: 994 6003
AclI AA'CG_TT
Cuts at: 1563 5124 5497 1563
Size: 3561 373 3063
Fragments arranged by size:
3561 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3057 3566 3737 4052 4073 5060 6444 6712
Size: 1425 509 171 315 21 987 1384 268
Cuts at: 6712 1632
Size: 1917
Fragments arranged by size:
1917 1425 1384 987 509 315 268 171
21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6800
AflIII A'CryG_T
Cuts at: 1203 5896 6873 1203
Size: 4693 977 1327
Fragments arranged by size:
4693 1327 977
AgeI A'CCGG_T
Cuts at: 3470 5828 3470
Size: 2358 4639
AhdI GACnn_n'nnGTC
Cuts at: 2173 3594 5726 2173
Size: 1421 2132 3444
Fragments arranged by size:
3444 2132 1421
AleI CACnn'nnGTG
Cuts at: 1166 3014 1166
Size: 1848 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3032 3989 1184
Size: 1848 957 4192
Fragments arranged by size:
4192 1848 957
AlwNI CAG_nnn'CTG
Cuts at: 3559 3673 3757 3883 4740 6312 3559
Size: 114 84 126 857 1572 4244
Fragments arranged by size:
4244 1572 857 126 114 84
ApaI G_GGCC'C
Cuts at: 2073 2129 2345 3346 3395 2073
Size: 56 216 1001 49 5675
Fragments arranged by size:
5675 1001 216 56 49
ApaLI G'TGCA_C
Cuts at: 3017 3515 5054 6210 3017
Size: 498 1539 1156 3804
Fragments arranged by size:
3804 1539 1156 498
ApoI r'AATT_y
Cuts at: 1493 4306 4532 4684 6793 1493
Size: 2813 226 152 2109 1697
Fragments arranged by size:
2813 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5551 160
Size: 872 4519 1606
Fragments arranged by size:
4519 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2179 2201 2248 2276 2338 2408
Size: 73 107 129 22 47 28 62 70
Cuts at: 2408 2555 3377 3996 4170 4322 1870
Size: 147 822 619 174 152 4545
Fragments arranged by size:
4545 822 619 174 152 147 129 107
73 70 62 47 28 22
BamHI G'GATC_C
Cuts at: 2761 4327 4694 2761
Size: 1566 367 5064
Fragments arranged by size:
5064 1566 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3113 3324 3846 4106 4318
Size: 325 217 627 1326 211 522 260 212
Cuts at: 4318 5773 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1326 627 522 325 260 217
212 211
BanII G_rGCy'C
Cuts at: 1391 2057 2073 2129 2330 2345 2409 2613 2775
Size: 666 16 56 201 15 64 204 162
Cuts at: 2775 2883 2960 3105 3346 3384 3395 3609 4182
Size: 108 77 145 241 38 11 214 573
Cuts at: 4182 4316 1391
Size: 134 4072
Fragments arranged by size:
4072 666 573 241 214 204 201 162
145 134 108 77 64 56 38 16
15 11
BbeI G_GCGC'C
Cuts at: 4110 4110
Size: 6997
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2494 2799 3415 928
Size: 971 595 305 616 4510
Fragments arranged by size:
4510 971 616 595 305
BbvCI CC'TCA_GC
Cuts at: 2582 4094 2582
Size: 1512 5485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3095 3129 3625 3659 5188 5222 1903
Size: 34 1158 34 496 34 1529 34 3678
Fragments arranged by size:
3678 1529 1158 496 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4889 6099 4889
Size: 1210 5787
BclI T'GATC_A
Cuts at: 1548 2781 1548
Size: 1233 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2068 2089
Size: 107 122 71 631 605 367 29 21
Cuts at: 2089 2098 2243 5608 136
Size: 9 145 3365 1525
Fragments arranged by size:
3365 1525 631 605 367 145 122 107
71 29 21 9
BglII A'GATC_T
Cuts at: 6992 6992
Size: 6997
BlpI GC'TnA_GC
Cuts at: 4414 4414
Size: 6997
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2129 2345 3021 3075 3118 3346 3395
Size: 283 56 216 676 54 43 228 49
Cuts at: 3395 3519 5058 6214 1790
Size: 124 1539 1156 2573
Fragments arranged by size:
2573 1539 1156 676 283 228 216 124
56 54 49 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6997
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3279 4728
Size: 416 53 83 399 24 1343 519 1449
Cuts at: 4728 5686 442
Size: 958 1753
Fragments arranged by size:
1753 1449 1343 958 519 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4177 4196 4177
Size: 19 6978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2564 3289 3383 3464 3554 3758 3929
Size: 1037 288 725 94 81 90 204 171
Cuts at: 3929 3953 4031 4301 5657 1239
Size: 24 78 270 1356 2579
Fragments arranged by size:
2579 1356 1037 725 288 270 204 171
94 90 81 78 24
Bpu10I CC'TnA_GC
Cuts at: 2582 3024 4094 2582
Size: 442 1070 5485
Fragments arranged by size:
5485 1070 442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3911 5122 5987 6285 3911
Size: 1211 865 298 4623
Fragments arranged by size:
4623 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2265 2978 3227 3581 4256 5660 882
Size: 617 766 713 249 354 675 1404 2219
Fragments arranged by size:
2219 1404 766 713 675 617 354 249
BsaAI yAC'GTr
Cuts at: 493 1204 4294 4788 493
Size: 711 3090 494 2702
Fragments arranged by size:
3090 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6991 6991
Size: 6997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2334 2499 3090
Size: 53 83 186 936 494 307 165 591
Cuts at: 3090 3595 4107 4804 5186 5821 275
Size: 505 512 697 382 635 1451
Fragments arranged by size:
1451 936 697 635 591 512 505 494
382 307 186 165 83 53
BsaWI w'CCGG_w
Cuts at: 3470 3696 5430 5828 6102 6249 6660 3470
Size: 226 1734 398 274 147 411 3807
Fragments arranged by size:
3807 1734 411 398 274 226 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2139 2169 2942 2972 3596
Size: 30 165 30 736 30 773 30 624
Cuts at: 3596 3626 4274 4304 1178
Size: 30 648 30 3871
Fragments arranged by size:
3871 773 736 648 624 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2426 2891 3113 3197 3413 3551 3695
Size: 1231 9 465 222 84 216 138 144
Cuts at: 3695 3755 3758 3829 3914 4082 4220 1186
Size: 60 3 71 85 168 138 3963
Fragments arranged by size:
3963 1231 465 222 216 168 144 138
138 85 84 71 60 9 3
BseYI C'CCAG_C
Cuts at: 3253 6200 6696 3253
Size: 2947 496 3554
Fragments arranged by size:
3554 2947 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3314 3688 1163
Size: 573 1578 374 4472
Fragments arranged by size:
4472 1578 573 374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3069 4447 5208 6236 1540
Size: 275 186 1068 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2969 3021 3189 3384 3519 3609 3915 4182 5058
Size: 52 168 195 135 90 306 267 876
Cuts at: 5058 5143 6214 2969
Size: 85 1071 3752
Fragments arranged by size:
3752 1071 876 306 267 195 168 135
90 85 52
BsmI GAATG_Cn'
Cuts at: 4508 4601 4508
Size: 93 6904
BsmBI CGTCTCn'nnnn_
Cuts at: 4171 4171
Size: 6997
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2073 2129 2330 2345 2409 2613
Size: 399 267 16 56 201 15 64 204
Cuts at: 2613 2775 2883 2960 2969 3021 3075 3105 3118
Size: 162 108 77 9 52 54 30 13
Cuts at: 3118 3189 3346 3384 3395 3519 3609 3915 4182
Size: 71 157 38 11 124 90 306 267
Cuts at: 4182 4316 5058 5143 6214 1391
Size: 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 267 267 204
201 162 157 134 124 108 90 85
77 71 64 56 54 52 38 30
16 15 13 11 9
BspEI T'CCGG_A
Cuts at: 6660 6660
Size: 6997
BspHI T'CATG_A
Cuts at: 4886 6616 4886
Size: 1730 5267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2725 3336 3513 3660 3924 4352 844
Size: 1881 611 177 147 264 428 3489
Fragments arranged by size:
3489 1881 611 428 264 177 147
BsrBI CCG'CTC
Cuts at: 1394 1823 4884 1394
Size: 429 3061 3507
Fragments arranged by size:
3507 3061 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3405 5492 5666 65
Size: 1183 2157 2087 174 1396
Fragments arranged by size:
2157 2087 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2242 3056 3470 5641 5828 1700
Size: 110 228 204 814 414 2171 187 2869
Fragments arranged by size:
2869 2171 814 414 228 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6997
BssHII G'CGCG_C
Cuts at: 1925 2442 1925
Size: 517 6480
BssSI C'ACGA_G
Cuts at: 3839 5057 6069 3839
Size: 1218 1012 4767
Fragments arranged by size:
4767 1218 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3053 3514 794
Size: 2259 461 4277
Fragments arranged by size:
4277 2259 461
BstBI TT'CG_AA
Cuts at: 2007 5801 2007
Size: 3794 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6997
BstXI CCAn_nnnn'nTGG
Cuts at: 3013 3013
Size: 6997
BstYI r'GATC_y
Cuts at: 1972 2761 2830 4327 4694 5079 5096 6548 6634
Size: 789 69 1497 367 385 17 1452 86
Cuts at: 6634 6646 6992 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1497 1452 789 385 367 346 86
69 17 12
Bsu36I CC'TnA_GG
Cuts at: 3491 3491
Size: 6997
BtgI C'CryG_G
Cuts at: 513 1065 2022 2309 2523 2613 3093 3109 3148
Size: 552 957 287 214 90 480 16 39
Cuts at: 3148 3351 513
Size: 203 4159
Fragments arranged by size:
4159 957 552 480 287 214 203 90
39 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6258
Fragments arranged by size:
6258 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 3268 4513 5307 5327 3217
Size: 51 1245 794 20 4887
Fragments arranged by size:
4887 1245 794 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6997
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6962
DraI TTT'AAA
Cuts at: 4301 4301
Size: 6997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2684 3419 4270 6004 809
Size: 507 1368 735 851 1734 1802
Fragments arranged by size:
1802 1734 1368 851 735 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3066 3151
Size: 54 1425 50 275 18 1009 227 85
Cuts at: 3151 4251 4444 5333 6962 8
Size: 1100 193 889 1629 43
Fragments arranged by size:
1629 1425 1100 1009 889 275 227 193
85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3066 4444 1537
Size: 275 1254 1378 4090
Fragments arranged by size:
4090 1378 1254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3579 3626 3792 4124 4927 1353
Size: 2226 47 166 332 803 3423
Fragments arranged by size:
3423 2226 803 332 166 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2275 3233 4208 5584 5958 6104
Size: 1064 138 686 958 975 1376 374 146
Cuts at: 6104 387
Size: 1280
Fragments arranged by size:
1376 1280 1064 975 958 686 374 146
138
EcoICRI GAG'CTC
Cuts at: 3382 3607 4180 3382
Size: 225 573 6199
Fragments arranged by size:
6199 573 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2564 3057 3289 3383 3464 3554
Size: 393 644 288 493 232 94 81 90
Cuts at: 3554 3566 3737 3758 3929 3953 4031 4052 4073
Size: 12 171 21 171 24 78 21 21
Cuts at: 4073 4301 5060 5657 6444 6712 1239
Size: 228 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 644 597 493 393 288
268 232 228 171 171 94 90 81
78 24 21 21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3494 3494
Size: 6997
EcoO109I rG'GnC_Cy
Cuts at: 2069 2126 2552 2559 3342 3343 3358 3367 3391
Size: 57 426 7 783 1 15 9 24
Cuts at: 3391 3892 2069
Size: 501 5174
Fragments arranged by size:
5174 783 501 426 57 24 15 9
7 1
EcoRI G'AATT_C
Cuts at: 4306 4684 4306
Size: 378 6619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2384 3439 4135 2384
Size: 1055 696 5246
Fragments arranged by size:
5246 1055 696
FseI GG_CCGG'CC
Cuts at: 2246 2246
Size: 6997
FspI TGC'GCA
Cuts at: 5503 5503
Size: 6997
HaeII r_GCGC'y
Cuts at: 1678 2153 2238 2366 2543 4110 6144 1678
Size: 475 85 128 177 1567 2034 2531
Fragments arranged by size:
2531 2034 1567 475 177 128 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2159 2171 2191 2489
Size: 32 135 32 567 20 12 20 298
Cuts at: 2489 2521 3580 3612 3758 3790 5638 5670 5712
Size: 32 1059 32 146 32 1848 32 42
Cuts at: 5712 5744 1373
Size: 32 2626
Fragments arranged by size:
2626 1848 1059 567 298 146 135 42
32 32 32 32 32 32 32 20
20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 4341 4587 669
Size: 988 281 559 1844 246 3079
Fragments arranged by size:
3079 1844 988 559 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6997
HpaI GTT'AAC
Cuts at: 4587 4587
Size: 6997
KasI G'GCGC_C
Cuts at: 4106 4106
Size: 6997
KpnI G_GTAC'C
Cuts at: 3328 4322 3328
Size: 994 6003
MfeI C'AATT_G
Cuts at: 4596 4596
Size: 6997
MluI A'CGCG_T
Cuts at: 6873 6873
Size: 6997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2210 3314 3741 6111 6295 1305
Size: 905 1104 427 2370 184 2007
Fragments arranged by size:
2370 2007 1104 905 427 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2841 3153 4253 10
Size: 54 1425 343 1009 312 1100 2754
Fragments arranged by size:
2754 1425 1100 1009 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3014 3147 3167 4955 5314 5473 518
Size: 648 1848 133 20 1788 359 159 2042
Fragments arranged by size:
2042 1848 1788 648 359 159 133 20
MspA1I CmG'CkG
Cuts at: 1606 2210 2311 3253 3511 3544 3907 3946 4413
Size: 604 101 942 258 33 363 39 467
Cuts at: 4413 5090 6238 6483 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 942 677 604 467 363 258
245 101 39 33
NaeI GCC'GGC
Cuts at: 2040 2244 3058 2040
Size: 204 814 5979
Fragments arranged by size:
5979 814 204
NarI GG'CG_CC
Cuts at: 4107 4107
Size: 6997
NcoI C'CATG_G
Cuts at: 513 1065 2022 3093 3109 3148 3351 513
Size: 552 957 1071 16 39 203 4159
Fragments arranged by size:
4159 1071 957 552 203 39 16
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6997
NgoMIV G'CCGG_C
Cuts at: 2038 2242 3056 2038
Size: 204 814 5979
Fragments arranged by size:
5979 814 204
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6997
NotI GC'GGCC_GC
Cuts at: 4444 4444
Size: 6997
NspI r_CATG'y
Cuts at: 1132 1229 4355 5900 1132
Size: 97 3126 1545 2229
Fragments arranged by size:
3126 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2345 1348
Size: 997 6000
PciI A'CATG_T
Cuts at: 5896 5896
Size: 6997
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6997
PfoI T'CCnGG_A
Cuts at: 2393 3426 3579 4278 2393
Size: 1033 153 699 5112
Fragments arranged by size:
5112 1033 699 153
PmeI GTTT'AAAC
Cuts at: 4301 4301
Size: 6997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5066 5098 6579 6611 5066
Size: 32 1481 32 5452
Fragments arranged by size:
5452 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2552 2559 3367 3892 2552
Size: 7 808 525 5657
Fragments arranged by size:
5657 808 525 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6997
PsiI TTA'TAA
Cuts at: 4567 4567
Size: 6997
PspOMI G'GGCC_C
Cuts at: 2069 2125 2341 3342 3391 2069
Size: 56 216 1001 49 5675
Fragments arranged by size:
5675 1001 216 56 49
PstI C_TGCA'G
Cuts at: 830 2215 3263 3510 3657 3921 3978 4153 4227
Size: 1385 1048 247 147 264 57 175 74
Cuts at: 4227 4349 830
Size: 122 3478
Fragments arranged by size:
3478 1385 1048 264 247 175 147 122
74 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6997
PvuII CAG'CTG
Cuts at: 3253 3511 3544 3907 3253
Size: 258 33 363 6343
Fragments arranged by size:
6343 363 258 33
SacI G_AGCT'C
Cuts at: 3384 3609 4182 3384
Size: 225 573 6199
Fragments arranged by size:
6199 573 225
SacII CC_GC'GG
Cuts at: 2312 2312
Size: 6997
SalI G'TCGA_C
Cuts at: 1936 2495 4339 1936
Size: 559 1844 4594
Fragments arranged by size:
4594 1844 559
SanDI GG'GwC_CC
Cuts at: 2559 2559
Size: 6997
SapI GCTCTTCn'nnn_
Cuts at: 3626 3792 3626
Size: 166 6831
SbfI CC_TGCA'GG
Cuts at: 3978 4227 4349 3978
Size: 249 122 6626
Fragments arranged by size:
6626 249 122
ScaI AGT'ACT
Cuts at: 2756 5245 6957 2756
Size: 2489 1712 2796
Fragments arranged by size:
2796 2489 1712
SfcI C'TryA_G
Cuts at: 826 1045 2211 2971 3259 3506 3653 3665 3917
Size: 219 1166 760 288 247 147 12 252
Cuts at: 3917 3974 4149 4223 4345 5480 6161 6352 826
Size: 57 175 74 122 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 760 681 288 252 247
219 191 175 147 122 74 57 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2039 2098 2039
Size: 59 6938
SfoI GGC'GCC
Cuts at: 4108 4108
Size: 6997
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
SgrAI Cr'CCGG_yG
Cuts at: 1700 3470 1700
Size: 1770 5227
SmaI CCC'GGG
Cuts at: 1872 2052 2181 2203 2250 2278 2340 2557 3998
Size: 180 129 22 47 28 62 217 1441
Cuts at: 3998 4324 1872
Size: 326 4545
Fragments arranged by size:
4545 1441 326 217 180 129 62 47
28 22
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3926 5101 6002 6264 820
Size: 197 926 1983 1175 901 262 1553
Fragments arranged by size:
1983 1553 1175 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4294 4788 493
Size: 3801 494 2702
Fragments arranged by size:
3801 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6997
SphI G_CATG'C
Cuts at: 1132 4355 1132
Size: 3223 3774
SspI AAT'ATT
Cuts at: 5 52 4921 5
Size: 47 4869 2081
Fragments arranged by size:
4869 2081 47
StuI AGG'CCT
Cuts at: 1517 3139 1517
Size: 1622 5375
StyI C'CwwG_G
Cuts at: 513 1065 2022 2803 3093 3109 3148 3351 4436
Size: 552 957 781 290 16 39 203 1085
Cuts at: 4436 513
Size: 3074
Fragments arranged by size:
3074 1085 957 781 552 290 203 39
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3890 5024 5041 5194 5379 5824 325
Size: 1255 2310 1134 17 153 185 445 1498
Fragments arranged by size:
2310 1498 1255 1134 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 5243 6955
Size: 274 80 33 51 1443 777 2489 1712
Cuts at: 6955 96
Size: 138
Fragments arranged by size:
2489 1712 1443 777 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3271 3303 3596 3628 6957 6989 3271
Size: 32 293 32 3329 32 3279
Fragments arranged by size:
3329 3279 293 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2912 1316
Size: 1596 5401
XbaI T'CTAG_A
Cuts at: 4333 4333
Size: 6997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 3313 3358 2849
Size: 464 45 6488
Fragments arranged by size:
6488 464 45
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6997
XmaI C'CCGG_G
Cuts at: 1870 2050 2179 2201 2248 2276 2338 2555 3996
Size: 180 129 22 47 28 62 217 1441
Cuts at: 3996 4322 1870
Size: 326 4545
Fragments arranged by size:
4545 1441 326 217 180 129 62 47
28 22
XmnI GAAnn'nnTTC
Cuts at: 5126 5126
Size: 6997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2500 4805 276
Size: 53 83 186 936 966 2305 2468
Fragments arranged by size:
2468 2305 966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SexAI SrfI SwaI