Restriction map for
pFN21ASDA0662
(Circular) (Six-base) MAPSORT of: pFN21ASDA0662.seq Check: 2088 from: 1 to: 8032
pFN21ASDA0662 8032 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3159 3159
Size: 8032
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2259 2469 5842 278
Size: 53 83 186 936 723 210 3373 2468
Fragments arranged by size:
3373 2468 936 723 210 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2919 4584 5375 1937
Size: 982 1665 791 4594
Fragments arranged by size:
4594 1665 982 791
Acc65I G'GTAC_C
Cuts at: 2796 5353 2796
Size: 2557 5475
AclI AA'CG_TT
Cuts at: 1563 6159 6532 1563
Size: 4596 373 3063
Fragments arranged by size:
4596 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 2564 3605 3884 4580 6095 7479 7747
Size: 714 218 1041 279 696 1515 1384 268
Cuts at: 7747 1632
Size: 1917
Fragments arranged by size:
1917 1515 1384 1041 714 696 279 268
218
AfeI AGC'GCT
Cuts at: 2870 2870
Size: 8032
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7835
AflIII A'CryG_T
Cuts at: 1203 4059 6931 7908 1203
Size: 2856 2872 977 1327
Fragments arranged by size:
2872 2856 1327 977
AgeI A'CCGG_T
Cuts at: 6863 6863
Size: 8032
AhdI GACnn_n'nnGTC
Cuts at: 6761 6761
Size: 8032
AleI CACnn'nnGTG
Cuts at: 1166 2999 1166
Size: 1833 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4571 1184
Size: 3387 4645
AlwNI CAG_nnn'CTG
Cuts at: 5123 5775 7347 5123
Size: 652 1572 5808
Fragments arranged by size:
5808 1572 652
ApaI G_GGCC'C
Cuts at: 5143 5143
Size: 8032
ApaLI G'TGCA_C
Cuts at: 6089 7245 6089
Size: 1156 6876
ApoI r'AATT_y
Cuts at: 1493 3222 3792 5289 5298 5341 5567 5719 7828
Size: 1729 570 1497 9 43 226 152 2109
Cuts at: 7828 1493
Size: 1697
Fragments arranged by size:
2109 1729 1697 1497 570 226 152 43
9
AseI AT'TA_AT
Cuts at: 160 1032 6586 160
Size: 872 5554 1606
Fragments arranged by size:
5554 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8032
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2239 2606 3624 5357 1870
Size: 73 172 124 367 1018 1733 4545
Fragments arranged by size:
4545 1733 1018 367 172 124 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4785 4818 4785
Size: 33 7999
BamHI G'GATC_C
Cuts at: 5362 5729 5362
Size: 367 7665
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2146 2783 2796 4508
Size: 325 217 627 245 114 637 13 1712
Cuts at: 4508 4762 4946 5126 5353 6808 618
Size: 254 184 180 227 1455 1842
Fragments arranged by size:
1842 1712 1455 637 627 325 254 245
227 217 184 180 114 13
BanII G_rGCy'C
Cuts at: 1391 2400 3288 3438 3989 4080 4301 4493 5073
Size: 1009 888 150 551 91 221 192 580
Cuts at: 5073 5143 5351 1391
Size: 70 208 4072
Fragments arranged by size:
4072 1009 888 580 551 221 208 192
150 91 70
BbeI G_GCGC'C
Cuts at: 2150 2787 2150
Size: 637 7395
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2334 2825 4680 928
Size: 971 435 491 1855 4280
Fragments arranged by size:
4280 1855 971 491 435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6223 6257 1903
Size: 34 4286 34 3678
Fragments arranged by size:
4286 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5924 7134 5924
Size: 1210 6822
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8032
BfrBI ATG'CAT
Cuts at: 2699 2699
Size: 8032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3568 6643 136
Size: 107 122 71 631 605 1896 3075 1525
Fragments arranged by size:
3075 1896 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8027 8027
Size: 8032
BlpI GC'TnA_GC
Cuts at: 5449 5449
Size: 8032
Bme1580I G_kGCm'C
Cuts at: 1790 2037 3747 4513 5143 6093 7249 1790
Size: 247 1710 766 630 950 1156 2573
Fragments arranged by size:
2573 1710 1156 950 766 630 247
BmgBI CAC'GTC
Cuts at: 1318 2332 1318
Size: 1014 7018
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3101 5763 6721
Size: 416 53 83 399 24 1684 2662 958
Cuts at: 6721 442
Size: 1753
Fragments arranged by size:
2662 1753 1684 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8032
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3652 5077 3373
Size: 279 1425 6328
Fragments arranged by size:
6328 1425 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3176 3497 4373 4682 6692 1239
Size: 1937 321 876 309 2010 2579
Fragments arranged by size:
2579 2010 1937 876 321 309
Bpu10I CC'TnA_GC
Cuts at: 3558 3558
Size: 8032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 4064 6157 7022 7320 2792
Size: 1272 2093 865 298 3504
Fragments arranged by size:
3504 2093 1272 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 6695 882
Size: 617 3309 1887 2219
Fragments arranged by size:
3309 2219 1887 617
BsaAI yAC'GTr
Cuts at: 493 1204 2479 2803 4060 4464 5329 5823 493
Size: 711 1275 324 1257 404 865 494 2702
Fragments arranged by size:
2702 1275 1257 865 711 494 404 324
BsaBI GATnn'nnATC
Cuts at: 8026 8026
Size: 8032
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2256 2466 2784
Size: 53 83 186 936 614 109 210 318
Cuts at: 2784 5839 6221 6856 275
Size: 3055 382 635 1451
Fragments arranged by size:
3055 1451 936 635 614 382 318 210
186 109 83 53
BsaWI w'CCGG_w
Cuts at: 3373 6465 6863 7137 7284 7695 3373
Size: 3092 398 274 147 411 3710
Fragments arranged by size:
3710 3092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3015 3045 3425 3455 3470
Size: 30 165 30 1612 30 380 30 15
Cuts at: 3470 3500 4898 4928 1178
Size: 30 1398 30 4282
Fragments arranged by size:
4282 1612 1398 380 165 30 30 30
30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 3446 3890 3944 4210 4352 4871 1186
Size: 1207 1053 444 54 266 142 519 4347
Fragments arranged by size:
4347 1207 1053 519 444 266 142 54
BseYI C'CCAG_C
Cuts at: 2365 2425 4364 4388 4848 5160 7235 7731 2365
Size: 60 1939 24 460 312 2075 496 2666
Fragments arranged by size:
2666 2075 1939 496 460 312 60 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 4223 4563 1163
Size: 573 552 1935 340 4632
Fragments arranged by size:
4632 1935 573 552 340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3245 3374 4267 4700 5268 5482
Size: 275 186 1244 129 893 433 568 214
Cuts at: 5482 6243 7271 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1244 1028 893 761 568 433 275
214 186 129
BsiHKAI G_wGCw'C
Cuts at: 2385 2808 3288 3297 4080 4301 4493 6093 6178
Size: 423 480 9 783 221 192 1600 85
Cuts at: 6178 7249 2385
Size: 1071 3168
Fragments arranged by size:
3168 1600 1071 783 480 423 221 192
85 9
BsiWI C'GTAC_G
Cuts at: 5246 5246
Size: 8032
BsmI GAATG_Cn'
Cuts at: 3161 3358 4419 5543 5636 3161
Size: 197 1061 1124 93 5557
Fragments arranged by size:
5557 1124 1061 197 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3447 5217 3447
Size: 1770 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2385 2400 2808 3288 3297 3438
Size: 399 247 348 15 408 480 9 141
Cuts at: 3438 3747 3989 4080 4301 4493 4513 5073 5143
Size: 309 242 91 221 192 20 560 70
Cuts at: 5143 5351 6093 6178 7249 1391
Size: 208 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 480 408 399 348
309 247 242 221 208 192 141 91
85 70 20 15 9
BspEI T'CCGG_A
Cuts at: 7695 7695
Size: 8032
BspHI T'CATG_A
Cuts at: 5921 7651 5921
Size: 1730 6302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3159 3669 4329 4440 5387 844
Size: 2315 510 660 111 947 3489
Fragments arranged by size:
3489 2315 947 660 510 111
BsrBI CCG'CTC
Cuts at: 1394 1823 5919 1394
Size: 429 4096 3507
Fragments arranged by size:
4096 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2699 4276 6527 6701 65
Size: 1183 1451 1577 2251 174 1396
Fragments arranged by size:
2251 1577 1451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 2839 4943 4999 6676 6863 1700
Size: 110 242 787 2104 56 1677 187 2869
Fragments arranged by size:
2869 2104 1677 787 242 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8032
BssHII G'CGCG_C
Cuts at: 1925 4347 1925
Size: 2422 5610
BssSI C'ACGA_G
Cuts at: 6092 7104 6092
Size: 1012 7020
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8032
BstBI TT'CG_AA
Cuts at: 2007 6836 2007
Size: 4829 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8032
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 2638
Size: 8032
BstYI r'GATC_y
Cuts at: 1972 2979 3336 5362 5729 6114 6131 7583 7669
Size: 1007 357 2026 367 385 17 1452 86
Cuts at: 7669 7681 8027 1972
Size: 12 346 1977
Fragments arranged by size:
2026 1977 1452 1007 385 367 357 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2971 2971
Size: 8032
BtgI C'CryG_G
Cuts at: 513 1065 2022 2191 4422 4504 5166 513
Size: 552 957 169 2231 82 662 3379
Fragments arranged by size:
3379 2231 957 662 552 169 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7293
Fragments arranged by size:
7293 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 4537 5548 6342 6362 2167
Size: 2370 1011 794 20 3837
Fragments arranged by size:
3837 2370 1011 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7997
DraI TTT'AAA
Cuts at: 5336 5336
Size: 8032
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7039 809
Size: 507 5723 1802
Fragments arranged by size:
5723 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2687 2706 2847
Size: 54 1425 50 275 18 857 19 141
Cuts at: 2847 3327 4264 4425 4474 5031 5149 5479 6368
Size: 480 937 161 49 557 118 330 889
Cuts at: 6368 7997 8
Size: 1629 43
Fragments arranged by size:
1629 1425 937 889 857 557 480 330
275 161 141 118 54 50 49 43
19 18
EagI C'GGCC_G
Cuts at: 1537 1812 4264 5479 1537
Size: 275 2452 1215 4090
Fragments arranged by size:
4090 2452 1215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2966 3867 4556 4599 5962 1353
Size: 938 675 901 689 43 1363 3423
Fragments arranged by size:
3423 1363 938 901 689 675 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5117 6619 6993 7139 387
Size: 1064 138 3528 1502 374 146 1280
Fragments arranged by size:
3528 1502 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3286 4078 4299 4491 3286
Size: 792 221 192 6827
Fragments arranged by size:
6827 792 221 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2564 3176 3497 3605 3884 4373
Size: 393 714 218 612 321 108 279 489
Cuts at: 4373 4580 4682 6095 6692 7479 7747 1239
Size: 207 102 1413 597 787 268 1524
Fragments arranged by size:
1524 1413 787 714 612 597 489 393
321 279 268 218 207 108 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2317 3155 4325 2317
Size: 838 1170 6024
Fragments arranged by size:
6024 1170 838
EcoO109I rG'GnC_Cy
Cuts at: 2587 3570 3975 4002 5139 2587
Size: 983 405 27 1137 5480
Fragments arranged by size:
5480 1137 983 405 27
EcoRI G'AATT_C
Cuts at: 5341 5719 5341
Size: 378 7654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 8032
FseI GG_CCGG'CC
Cuts at: 2843 2843
Size: 8032
FspI TGC'GCA
Cuts at: 6538 6538
Size: 8032
HaeII r_GCGC'y
Cuts at: 1678 2150 2252 2787 2872 7179 1678
Size: 472 102 535 85 4307 2531
Fragments arranged by size:
4307 2531 535 472 102 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2812 2844 3015 3047 3425
Size: 32 135 32 1240 32 171 32 378
Cuts at: 3425 3457 3470 3502 4046 4078 4205 4237 6673
Size: 32 13 32 544 32 127 32 2436
Cuts at: 6673 6705 6747 6779 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2436 1240 544 378 171 135 127
42 32 32 32 32 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 4585 5376 5622 669
Size: 988 281 982 1665 791 246 3079
Fragments arranged by size:
3079 1665 988 982 791 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8032
HpaI GTT'AAC
Cuts at: 5622 5622
Size: 8032
KasI G'GCGC_C
Cuts at: 2146 2783 2146
Size: 637 7395
KpnI G_GTAC'C
Cuts at: 2800 5357 2800
Size: 2557 5475
MfeI C'AATT_G
Cuts at: 5631 5631
Size: 8032
MluI A'CGCG_T
Cuts at: 7908 7908
Size: 8032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3394 3966 4044 4125 4467 7146 7330 1305
Size: 2089 572 78 81 342 2679 184 2007
Fragments arranged by size:
2679 2089 2007 572 342 184 81 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2689 2708 3329 4427 4476
Size: 54 1425 343 857 19 621 1098 49
Cuts at: 4476 10
Size: 3566
Fragments arranged by size:
3566 1425 1098 857 621 343 54 49
19
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 2999 4620 5990 6349 6508 518
Size: 648 1458 375 1621 1370 359 159 2042
Fragments arranged by size:
2042 1621 1458 1370 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 3193 4307 5123 5231 5448 6125 7273
Size: 522 1065 1114 816 108 217 677 1148
Cuts at: 7273 7518 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1114 1065 816 677 522 245
217 108
NaeI GCC'GGC
Cuts at: 2054 2841 4945 2054
Size: 787 2104 5141
Fragments arranged by size:
5141 2104 787
NarI GG'CG_CC
Cuts at: 2147 2784 2147
Size: 637 7395
NcoI C'CATG_G
Cuts at: 513 1065 2022 2191 4422 4504 5166 513
Size: 552 957 169 2231 82 662 3379
Fragments arranged by size:
3379 2231 957 662 552 169 82
NdeI CA'TA_TG
Cuts at: 387 3067 387
Size: 2680 5352
NgoMIV G'CCGG_C
Cuts at: 2052 2839 4943 2052
Size: 787 2104 5141
Fragments arranged by size:
5141 2104 787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8032
NotI GC'GGCC_GC
Cuts at: 5479 5479
Size: 8032
NsiI A_TGCA'T
Cuts at: 2701 2701
Size: 8032
NspI r_CATG'y
Cuts at: 1132 1229 3769 5390 6935 1132
Size: 97 2540 1621 1545 2229
Fragments arranged by size:
2540 2229 1621 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2319 5174 1348
Size: 971 2855 4206
Fragments arranged by size:
4206 2855 971
PciI A'CATG_T
Cuts at: 6931 6931
Size: 8032
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5167 1108
Size: 4059 3973
PfoI T'CCnGG_A
Cuts at: 3288 3474 3288
Size: 186 7846
PmeI GTTT'AAAC
Cuts at: 5336 5336
Size: 8032
PmlI CAC'GTG
Cuts at: 2803 4464 2803
Size: 1661 6371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6101 6133 7614 7646 6101
Size: 32 1481 32 6487
Fragments arranged by size:
6487 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2587
Size: 8032
PshAI GACnn'nnGTC
Cuts at: 1613 2510 1613
Size: 897 7135
PsiI TTA'TAA
Cuts at: 5602 5602
Size: 8032
PspOMI G'GGCC_C
Cuts at: 5139 5139
Size: 8032
PspXI vC'TCGA_Gb
Cuts at: 2606 2606
Size: 8032
PstI C_TGCA'G
Cuts at: 830 4329 4437 4458 4837 5384 830
Size: 3499 108 21 379 547 3478
Fragments arranged by size:
3499 3478 547 379 108 21
PvuI CG_AT'CG
Cuts at: 2001 3245 2001
Size: 1244 6788
PvuII CAG'CTG
Cuts at: 3193 4307 3193
Size: 1114 6918
RsrII CG'GwC_CG
Cuts at: 2108 2108
Size: 8032
SacI G_AGCT'C
Cuts at: 3288 4080 4301 4493 3288
Size: 792 221 192 6827
Fragments arranged by size:
6827 792 221 192
SalI G'TCGA_C
Cuts at: 1936 2918 4583 5374 1936
Size: 982 1665 791 4594
Fragments arranged by size:
4594 1665 982 791
SapI GCTCTTCn'nnn_
Cuts at: 2291 4599 2291
Size: 2308 5724
SbfI CC_TGCA'GG
Cuts at: 4437 5384 4437
Size: 947 7085
ScaI AGT'ACT
Cuts at: 2729 6280 7992 2729
Size: 3551 1712 2769
Fragments arranged by size:
3551 2769 1712
SexAI A'CCwGG_T
Cuts at: 2913 2913
Size: 8032
SfcI C'TryA_G
Cuts at: 826 1045 2566 4325 4433 4454 4732 4833 5380
Size: 219 1521 1759 108 21 278 101 547
Cuts at: 5380 6515 7196 7387 826
Size: 1135 681 191 1471
Fragments arranged by size:
1759 1521 1471 1135 681 547 278 219
191 108 101 21
SfoI GGC'GCC
Cuts at: 2148 2785 2148
Size: 637 7395
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8032
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8032
SmaI CCC'GGG
Cuts at: 1872 2117 2241 3626 5359 1872
Size: 245 124 1385 1733 4545
Fragments arranged by size:
4545 1733 1385 245 124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2606 2807 4079 6136 7037 7299
Size: 197 926 663 201 1272 2057 901 262
Cuts at: 7299 820
Size: 1553
Fragments arranged by size:
2057 1553 1272 926 901 663 262 201
197
SnaBI TAC'GTA
Cuts at: 493 5329 5823 493
Size: 4836 494 2702
Fragments arranged by size:
4836 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8032
SphI G_CATG'C
Cuts at: 1132 5390 1132
Size: 4258 3774
SspI AAT'ATT
Cuts at: 5 52 5956 5
Size: 47 5904 2081
Fragments arranged by size:
5904 2081 47
StuI AGG'CCT
Cuts at: 1517 4439 4780 5045 1517
Size: 2922 341 265 4504
Fragments arranged by size:
4504 2922 341 265
StyI C'CwwG_G
Cuts at: 513 1065 2022 2191 3211 3566 3678 3963 4422
Size: 552 957 169 1020 355 112 285 459
Cuts at: 4422 4482 4504 5166 5471 513
Size: 60 22 662 305 3074
Fragments arranged by size:
3074 1020 957 662 552 459 355 305
285 169 112 60 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 3655 6059 6076 6229 6414 6859
Size: 1255 545 1530 2404 17 153 185 445
Cuts at: 6859 325
Size: 1498
Fragments arranged by size:
2404 1530 1498 1255 545 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 2727 3834
Size: 274 80 33 51 1443 63 687 1107
Cuts at: 3834 4356 5112 6278 7990 96
Size: 522 756 1166 1712 138
Fragments arranged by size:
1712 1443 1166 1107 756 687 522 274
138 80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3630 3662 7992 8024 3630
Size: 32 4330 32 3638
Fragments arranged by size:
4330 3638 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2915 1316
Size: 1599 6433
XbaI T'CTAG_A
Cuts at: 5368 5368
Size: 8032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2774 3652 3754 2774
Size: 878 102 7052
Fragments arranged by size:
7052 878 102
XhoI C'TCGA_G
Cuts at: 1943 2606 1943
Size: 663 7369
XmaI C'CCGG_G
Cuts at: 1870 2115 2239 3624 5357 1870
Size: 245 124 1385 1733 4545
Fragments arranged by size:
4545 1733 1385 245 124
XmnI GAAnn'nnTTC
Cuts at: 2822 4404 6161 2822
Size: 1582 1757 4693
Fragments arranged by size:
4693 1757 1582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2257 2467 5840 276
Size: 53 83 186 936 723 210 3373 2468
Fragments arranged by size:
3373 2468 936 723 210 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BstZ17I DraIII EcoRV FspAI
NruI PacI PsrI SacII SanDI SfiI SrfI SwaI