Restriction map for
pFN21ASDA0658
(Circular) (Six-base) MAPSORT of: pFN21ASDA0658.seq Check: 6748 from: 1 to: 6508
pFN21ASDA0658 6508 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3574 3851 1937
Size: 1637 277 4594
Fragments arranged by size:
4594 1637 277
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 4635 5008 1563
Size: 3072 373 3063
Fragments arranged by size:
3072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2706 4571 5955 6223 1632
Size: 599 475 1865 1384 268 1917
Fragments arranged by size:
1917 1865 1384 599 475 268
AfeI AGC'GCT
Cuts at: 2534 2534
Size: 6508
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 2325 5407 6384 1203
Size: 1122 3082 977 1327
Fragments arranged by size:
3082 1327 1122 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 2579 3472 1184
Size: 948 447 893 4220
Fragments arranged by size:
4220 948 893 447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 2789 2821 2660
Size: 32 97 32 6347
Fragments arranged by size:
6347 97 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2254 4251 5823 2254
Size: 1997 1572 2939
Fragments arranged by size:
2939 1997 1572
ApaI G_GGCC'C
Cuts at: 3757 3757
Size: 6508
ApaLI G'TGCA_C
Cuts at: 2096 4565 5721 2096
Size: 2469 1156 2883
Fragments arranged by size:
2883 2469 1156
ApoI r'AATT_y
Cuts at: 1493 2461 3817 4043 4195 6304 1493
Size: 968 1356 226 152 2109 1697
Fragments arranged by size:
2109 1697 1356 968 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 3634 3756 3833 1870
Size: 73 1691 122 77 4545
Fragments arranged by size:
4545 1691 122 77 73
BamHI G'GATC_C
Cuts at: 3135 3838 4205 3135
Size: 703 367 5438
Fragments arranged by size:
5438 703 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 3829 5284 618
Size: 325 217 627 275 1767 1455 1842
Fragments arranged by size:
1842 1767 1455 627 325 275 217
BanII G_rGCy'C
Cuts at: 1391 3420 3630 3757 3827 1391
Size: 2029 210 127 70 4072
Fragments arranged by size:
4072 2029 210 127 70
BbeI G_GCGC'C
Cuts at: 2066 2066
Size: 6508
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3609 928
Size: 971 1710 3827
Fragments arranged by size:
3827 1710 971
BbvCI CC'TCA_GC
Cuts at: 2831 3072 2831
Size: 241 6267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2435 2826 3068
Size: 107 122 71 631 605 763 391 242
Cuts at: 3068 3327 3645 3761 5119 136
Size: 259 318 116 1358 1525
Fragments arranged by size:
1525 1358 763 631 605 391 318 259
242 122 116 107 71
BglII A'GATC_T
Cuts at: 6503 6503
Size: 6508
BlpI GC'TnA_GC
Cuts at: 3925 3925
Size: 6508
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2658 3757 4569 5725 1790
Size: 310 558 1099 812 1156 2573
Fragments arranged by size:
2573 1156 1099 812 558 310
BmgBI CAC'GTC
Cuts at: 1318 2326 1318
Size: 1008 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 3675 4239
Size: 416 53 83 399 24 1928 330 564
Cuts at: 4239 5197 442
Size: 958 1753
Fragments arranged by size:
1928 1753 958 564 416 399 330 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3649 3649
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2684 5168 1239
Size: 1445 2484 2579
Fragments arranged by size:
2579 2484 1445
Bpu10I CC'TnA_GC
Cuts at: 2831 3072 2831
Size: 241 6267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2636 2779 3497 5171 882
Size: 617 1137 143 718 1674 2219
Fragments arranged by size:
2219 1674 1137 718 617 143
BsaAI yAC'GTr
Cuts at: 493 1204 3805 4299 493
Size: 711 2601 494 2702
Fragments arranged by size:
2702 2601 711 494
BsaBI GATnn'nnATC
Cuts at: 6502 6502
Size: 6508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 4315 4697 5332
Size: 53 83 186 936 530 2252 382 635
Cuts at: 5332 275
Size: 1451
Fragments arranged by size:
2252 1451 936 635 530 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4941 5339 5613 5760 6171 4941
Size: 398 274 147 411 5278
Fragments arranged by size:
5278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 2789 2819 1178
Size: 30 165 30 1257 30 99 30 4867
Fragments arranged by size:
4867 1257 165 99 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2200 2203 2681 2730 1186
Size: 1014 3 478 49 4964
Fragments arranged by size:
4964 1014 478 49 3
BseYI C'CCAG_C
Cuts at: 2671 3198 3629 3688 5711 6207 2671
Size: 527 431 59 2023 496 2972
Fragments arranged by size:
2972 2023 527 496 431 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3681 1163
Size: 573 1945 3990
Fragments arranged by size:
3990 1945 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 3958 4719 5747 1540
Size: 275 186 150 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 2100 4569 4654 5725 2100
Size: 2469 85 1071 2883
Fragments arranged by size:
2883 2469 1071 85
BsmI GAATG_Cn'
Cuts at: 3430 4019 4112 3430
Size: 589 93 5826
Fragments arranged by size:
5826 589 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2658 3420 3630 3757 3827 4569
Size: 399 310 558 762 210 127 70 742
Cuts at: 4569 4654 5725 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 762 742 558 399 310 210
127 85 70
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 2418 3108 4397 6127 2418
Size: 690 1289 1730 2799
Fragments arranged by size:
2799 1730 1289 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2850 3384 3863 844
Size: 2006 534 479 3489
Fragments arranged by size:
3489 2006 534 479
BsrBI CCG'CTC
Cuts at: 1394 1823 3207 4395 1394
Size: 429 1384 1188 3507
Fragments arranged by size:
3507 1384 1188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5003 5177 65
Size: 1183 3755 174 1396
Fragments arranged by size:
3755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3510 5152 5339 1700
Size: 110 1700 1642 187 2869
Fragments arranged by size:
2869 1700 1642 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6508
BssHII G'CGCG_C
Cuts at: 1925 2162 1925
Size: 237 6271
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6508
BstBI TT'CG_AA
Cuts at: 2007 5312 2007
Size: 3305 3203
BstEII G'GTnAC_C
Cuts at: 1184 2587 1184
Size: 1403 5105
BstXI CCAn_nnnn'nTGG
Cuts at: 2706 2706
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2622 3030 3135 3838 4205 4590 4607 6059
Size: 650 408 105 703 367 385 17 1452
Cuts at: 6059 6145 6157 6503 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 703 650 408 385 367 346
105 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3051 3051
Size: 6508
BtgI C'CryG_G
Cuts at: 513 1065 2022 2195 3159 3178 3479 513
Size: 552 957 173 964 19 301 3542
Fragments arranged by size:
3542 964 957 552 301 173 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 3310 3664 4024 4818 4838 3292
Size: 18 354 360 794 20 4962
Fragments arranged by size:
4962 794 360 354 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6473
DraI TTT'AAA
Cuts at: 3812 3812
Size: 6508
DraIII CAC_nnn'GTG
Cuts at: 3619 3734 3619
Size: 115 6393
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3191 5515 809
Size: 507 1875 2324 1802
Fragments arranged by size:
2324 1875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2427 2814
Size: 54 1425 50 275 18 318 279 387
Cuts at: 2814 3060 3334 3955 4844 6473 8
Size: 246 274 621 889 1629 43
Fragments arranged by size:
1629 1425 889 621 387 318 279 275
274 246 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 3955 1537
Size: 275 336 1807 4090
Fragments arranged by size:
4090 1807 336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2095 2876 4438 1353
Size: 742 781 1562 3423
Fragments arranged by size:
3423 1562 781 742
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5095 5469 5615 387
Size: 1064 138 3506 374 146 1280
Fragments arranged by size:
3506 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2684 2706 4571 5168 5955 6223
Size: 393 599 453 22 1865 597 787 268
Cuts at: 6223 1239
Size: 1524
Fragments arranged by size:
1865 1524 787 599 597 453 393 268
22
EcoO109I rG'GnC_Cy
Cuts at: 2890 3055 3184 2890
Size: 165 129 6214
Fragments arranged by size:
6214 165 129
EcoRI G'AATT_C
Cuts at: 2461 3817 4195 2461
Size: 1356 378 4774
Fragments arranged by size:
4774 1356 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 6508
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 2066 2072 2088 2536 5655 1678
Size: 388 6 16 448 3119 2531
Fragments arranged by size:
3119 2531 448 388 16 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2713 2745 5149
Size: 32 135 32 500 32 609 32 2404
Cuts at: 5149 5181 5223 5255 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2404 609 500 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3493 3852 4098 669
Size: 988 281 1555 359 246 3079
Fragments arranged by size:
3079 1555 988 359 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KasI G'GCGC_C
Cuts at: 2062 2062
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5622 5806 1305
Size: 4317 184 2007
Fragments arranged by size:
4317 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 2816 3062 10
Size: 54 1425 343 597 387 246 3456
Fragments arranged by size:
3456 1425 597 387 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3467 4466 4825 4984 518
Size: 648 1257 1044 999 359 159 2042
Fragments arranged by size:
2042 1257 1044 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2702 3180 3198 3267 3924 4601 5749
Size: 445 651 478 18 69 657 677 1148
Cuts at: 5749 5994 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 657 651 478 445 245
69 18
NaeI GCC'GGC
Cuts at: 3512 3512
Size: 6508
NarI GG'CG_CC
Cuts at: 2063 2063
Size: 6508
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4999
Fragments arranged by size:
4999 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NgoMIV G'CCGG_C
Cuts at: 3510 3510
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 3158 3866 5411 1132
Size: 97 1929 708 1545 2229
Fragments arranged by size:
2229 1929 1545 708 97
PasI CC'CwG_GG
Cuts at: 1348 3036 1348
Size: 1688 4820
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 3145 1108
Size: 1494 543 4471
Fragments arranged by size:
4471 1494 543
PfoI T'CCnGG_A
Cuts at: 2625 3234 2625
Size: 609 5899
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2789 2821 4577 4609 6090 6122 2660
Size: 32 97 32 1756 32 1481 32 3046
Fragments arranged by size:
3046 1756 1481 97 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2890 3184 2890
Size: 294 6214
PshAI GACnn'nnGTC
Cuts at: 1613 3609 1613
Size: 1996 4512
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PspOMI G'GGCC_C
Cuts at: 3753 3753
Size: 6508
PspXI vC'TCGA_Gb
Cuts at: 3634 3634
Size: 6508
PstI C_TGCA'G
Cuts at: 830 2615 3287 3860 830
Size: 1785 672 573 3478
Fragments arranged by size:
3478 1785 672 573
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2051 2702 3198 3267 2051
Size: 651 496 69 5292
Fragments arranged by size:
5292 651 496 69
SacII CC_GC'GG
Cuts at: 3181 3181
Size: 6508
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 4756 6468 4756
Size: 1712 4796
SexAI A'CCwGG_T
Cuts at: 3730 3730
Size: 6508
SfcI C'TryA_G
Cuts at: 826 1045 2611 3283 3856 4991 5672 5863 826
Size: 219 1566 672 573 1135 681 191 1471
Fragments arranged by size:
1566 1471 1135 681 672 573 219 191
SfoI GGC'GCC
Cuts at: 2064 2064
Size: 6508
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3758 3835 1872
Size: 1886 77 4545
Fragments arranged by size:
4545 1886 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3634 4612 5513 5775 820
Size: 197 926 1691 978 901 262 1553
Fragments arranged by size:
1691 1553 978 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3805 4299 493
Size: 3312 494 2702
Fragments arranged by size:
3312 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3158 3866 1132
Size: 2026 708 3774
Fragments arranged by size:
3774 2026 708
SspI AAT'ATT
Cuts at: 5 52 4432 5
Size: 47 4380 2081
Fragments arranged by size:
4380 2081 47
StuI AGG'CCT
Cuts at: 1517 2765 2829 1517
Size: 1248 64 5196
Fragments arranged by size:
5196 1248 64
StyI C'CwwG_G
Cuts at: 513 1065 2022 2333 2430 3092 3947 513
Size: 552 957 311 97 662 855 3074
Fragments arranged by size:
3074 957 855 662 552 311 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4535 4552 4705 4890 5335 325
Size: 1255 2955 17 153 185 445 1498
Fragments arranged by size:
2955 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4754 6466 96
Size: 274 80 33 51 1443 2777 1712 138
Fragments arranged by size:
2777 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6468 6500 6468
Size: 32 6476
Tth111I GACn'n_nGTC
Cuts at: 1316 2694 3570 1316
Size: 1378 876 4254
Fragments arranged by size:
4254 1378 876
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 2575 2599 2809 3706 2422
Size: 153 24 210 897 5224
Fragments arranged by size:
5224 897 210 153 24
XhoI C'TCGA_G
Cuts at: 1943 3634 1943
Size: 1691 4817
XmaI C'CCGG_G
Cuts at: 1870 3756 3833 1870
Size: 1886 77 4545
Fragments arranged by size:
4545 1886 77
XmnI GAAnn'nnTTC
Cuts at: 3392 4637 3392
Size: 1245 5263
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI
PmlI PsrI RsrII SacI SanDI SapI SfiI SrfI
SwaI