Restriction map for
pFN21ASDA0649
(Circular) (Six-base) MAPSORT of: pFN21ASDA0649.seq Check: 9826 from: 1 to: 8356
pFN21ASDA0649 8356 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6166 278
Size: 53 83 186 936 4630 2468
Fragments arranged by size:
4630 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5649 5699 1937
Size: 3712 50 4594
Fragments arranged by size:
4594 3712 50
Acc65I G'GTAC_C
Cuts at: 5677 5677
Size: 8356
AclI AA'CG_TT
Cuts at: 1563 6483 6856 1563
Size: 4920 373 3063
Fragments arranged by size:
4920 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2988 3392 4083 4292 5314 5362 5584
Size: 833 523 404 691 209 1022 48 222
Cuts at: 5584 6419 7803 8071 1632
Size: 835 1384 268 1917
Fragments arranged by size:
1917 1384 1022 835 833 691 523 404
268 222 209 48
AfeI AGC'GCT
Cuts at: 3049 3049
Size: 8356
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8159
AflIII A'CryG_T
Cuts at: 1203 7255 8232 1203
Size: 6052 977 1327
Fragments arranged by size:
6052 1327 977
AgeI A'CCGG_T
Cuts at: 7187 7187
Size: 8356
AhdI GACnn_n'nnGTC
Cuts at: 5206 5546 7085 5206
Size: 340 1539 6477
Fragments arranged by size:
6477 1539 340
AleI CACnn'nnGTG
Cuts at: 1166 2559 1166
Size: 1393 6963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2269 3010 4389 1184
Size: 1085 741 1379 5151
Fragments arranged by size:
5151 1379 1085 741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 8324
AlwNI CAG_nnn'CTG
Cuts at: 2722 2860 6099 7671 2722
Size: 138 3239 1572 3407
Fragments arranged by size:
3407 3239 1572 138
ApaI G_GGCC'C
Cuts at: 2488 3989 4262 4472 4610 4634 5219 5363 2488
Size: 1501 273 210 138 24 585 144 5481
Fragments arranged by size:
5481 1501 585 273 210 144 138 24
ApaLI G'TGCA_C
Cuts at: 2288 4687 6413 7569 2288
Size: 2399 1726 1156 3075
Fragments arranged by size:
3075 2399 1726 1156
ApoI r'AATT_y
Cuts at: 1493 2794 5665 5891 6043 8152 1493
Size: 1301 2871 226 152 2109 1697
Fragments arranged by size:
2871 2109 1697 1301 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6910 160
Size: 872 5878 1606
Fragments arranged by size:
5878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8356
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2202 2471 3269 4818 5032 5053
Size: 73 214 45 269 798 1549 214 21
Cuts at: 5053 5223 5503 5681 1870
Size: 170 280 178 4545
Fragments arranged by size:
4545 1549 798 280 269 214 214 178
170 73 45 21
BamHI G'GATC_C
Cuts at: 2630 4825 5686 6053 2630
Size: 2195 861 367 4933
Fragments arranged by size:
4933 2195 861 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 3974 4038 4373 4831
Size: 325 217 627 420 1767 64 335 458
Cuts at: 4831 4982 4997 5677 7132 618
Size: 151 15 680 1455 1842
Fragments arranged by size:
1842 1767 1455 680 627 458 420 335
325 217 151 64 15
BanII G_rGCy'C
Cuts at: 1391 2488 2651 3404 3912 3989 4061 4164 4236
Size: 1097 163 753 508 77 72 103 72
Cuts at: 4236 4262 4472 4610 4634 4803 4875 4919 5033
Size: 26 210 138 24 169 72 44 114
Cuts at: 5033 5080 5219 5363 5504 5510 5675 1391
Size: 47 139 144 141 6 165 4072
Fragments arranged by size:
4072 1097 753 508 210 169 165 163
144 141 139 138 114 103 77 72
72 72 47 44 26 24 6
BbeI G_GCGC'C
Cuts at: 4042 5001 4042
Size: 959 7397
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4071 928
Size: 971 2172 5213
Fragments arranged by size:
5213 2172 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2180 2214 6547 6581 1903
Size: 34 243 34 4333 34 3678
Fragments arranged by size:
4333 3678 243 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6248 7458 6248
Size: 1210 7146
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2270 2483 2865
Size: 107 122 71 631 605 598 213 382
Cuts at: 2865 3656 3747 3956 6967 136
Size: 791 91 209 3011 1525
Fragments arranged by size:
3011 1525 791 631 605 598 382 213
209 122 107 91 71
BglII A'GATC_T
Cuts at: 8351 8351
Size: 8356
BlpI GC'TnA_GC
Cuts at: 3459 3906 4929 5773 3459
Size: 447 1023 844 6042
Fragments arranged by size:
6042 1023 844 447
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2292 2488 2566 3977 3989 4262 4472
Size: 420 82 196 78 1411 12 273 210
Cuts at: 4472 4610 4634 4691 4769 4985 5219 5363 6417
Size: 138 24 57 78 216 234 144 1054
Cuts at: 6417 7573 1790
Size: 1156 2573
Fragments arranged by size:
2573 1411 1156 1054 420 273 234 216
210 196 144 138 82 78 78 57
24 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4326 6087 7045
Size: 416 53 83 399 24 2909 1761 958
Cuts at: 7045 442
Size: 1753
Fragments arranged by size:
2909 1761 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2028 2042 3091 3378 4559 5094 5122 7016
Size: 789 14 1049 287 1181 535 28 1894
Cuts at: 7016 1239
Size: 2579
Fragments arranged by size:
2579 1894 1181 1049 789 535 287 28
14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4127 6481 7346 7644 4127
Size: 2354 865 298 4839
Fragments arranged by size:
4839 2354 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2327 2738 3266 3921 4408 7019 882
Size: 617 828 411 528 655 487 2611 2219
Fragments arranged by size:
2611 2219 828 655 617 528 487 411
BsaAI yAC'GTr
Cuts at: 493 1204 5653 6147 493
Size: 711 4449 494 2702
Fragments arranged by size:
4449 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2171 8350 2171
Size: 6179 2177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 2868 3089 3098
Size: 53 83 186 936 729 606 221 9
Cuts at: 3098 4039 4998 5184 5574 5589 6163 6545 7180
Size: 941 959 186 390 15 574 382 635
Cuts at: 7180 275
Size: 1451
Fragments arranged by size:
1451 959 941 936 729 635 606 574
390 382 221 186 186 83 53 15
9
BsaWI w'CCGG_w
Cuts at: 3636 4236 4828 6789 7187 7461 7608 8019 3636
Size: 600 592 1961 398 274 147 411 3973
Fragments arranged by size:
3973 1961 600 592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2633 2663 3370
Size: 30 165 30 726 30 474 30 707
Cuts at: 3370 3400 3584 3614 1178
Size: 30 184 30 5920
Fragments arranged by size:
5920 726 707 474 184 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 2438 3143 3224 4426 5512 1186
Size: 1180 72 705 81 1202 1086 4030
Fragments arranged by size:
4030 1202 1180 1086 705 81 72
BseYI C'CCAG_C
Cuts at: 2487 2502 2863 3290 3790 4225 4471 4478 4633
Size: 15 361 427 500 435 246 7 155
Cuts at: 4633 4964 5048 5132 5234 5274 5399 7559 8055
Size: 331 84 84 102 40 125 2160 496
Cuts at: 8055 2487
Size: 2788
Fragments arranged by size:
2788 2160 500 496 435 427 361 331
246 155 125 102 84 84 40 15
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2517 3492 4174 4195 4675 1163
Size: 573 447 334 975 682 21 480 4844
Fragments arranged by size:
4844 975 682 573 480 447 334 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5089 5806 6567 7595 1540
Size: 275 186 3088 717 761 1028 2301
Fragments arranged by size:
3088 2301 1028 761 717 275 186
BsiHKAI G_wGCw'C
Cuts at: 2292 4061 4691 4762 4847 5504 6417 6502 7573
Size: 1769 630 71 85 657 913 85 1071
Cuts at: 7573 2292
Size: 3075
Fragments arranged by size:
3075 1769 1071 913 657 630 85 85
71
BsmI GAATG_Cn'
Cuts at: 5867 5960 5867
Size: 93 8263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2292 2488 2566 2651 3404 3912
Size: 399 420 82 196 78 85 753 508
Cuts at: 3912 3977 3989 4061 4164 4236 4262 4472 4610
Size: 65 12 72 103 72 26 210 138
Cuts at: 4610 4634 4691 4762 4769 4803 4847 4875 4919
Size: 24 57 71 7 34 44 28 44
Cuts at: 4919 4985 5033 5080 5219 5363 5504 5510 5675
Size: 66 48 47 139 144 141 6 165
Cuts at: 5675 6417 6502 7573 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 753 742 508 420 399 210
196 165 144 141 139 138 103 85
85 82 78 72 72 71 66 65
57 48 47 44 44 34 28 26
24 12 7 6
BspEI T'CCGG_A
Cuts at: 3636 4236 8019 3636
Size: 600 3783 3973
Fragments arranged by size:
3973 3783 600
BspHI T'CATG_A
Cuts at: 2031 3072 6245 7975 2031
Size: 1041 3173 1730 2412
Fragments arranged by size:
3173 2412 1730 1041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3192 3939 5711 844
Size: 2348 747 1772 3489
Fragments arranged by size:
3489 2348 1772 747
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 4616 5060 6243 1394
Size: 429 1759 1034 444 1183 3507
Fragments arranged by size:
3507 1759 1183 1034 444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6851 7025 65
Size: 1183 5603 174 1396
Fragments arranged by size:
5603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2269 2477 2548 2983 3508 3746 4666
Size: 110 459 208 71 435 525 238 920
Cuts at: 4666 7000 7187 1700
Size: 2334 187 2869
Fragments arranged by size:
2869 2334 920 525 459 435 238 208
187 110 71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8356
BssHII G'CGCG_C
Cuts at: 1925 2221 1925
Size: 296 8060
BssSI C'ACGA_G
Cuts at: 3436 4920 6416 7428 3436
Size: 1484 1496 1012 4364
Fragments arranged by size:
4364 1496 1484 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2860 794
Size: 2066 6290
BstBI TT'CG_AA
Cuts at: 2007 7160 2007
Size: 5153 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8356
BstXI CCAn_nnnn'nTGG
Cuts at: 3322 3322
Size: 8356
BstYI r'GATC_y
Cuts at: 1972 2630 4825 5686 6053 6438 6455 7907 7993
Size: 658 2195 861 367 385 17 1452 86
Cuts at: 7993 8005 8351 1972
Size: 12 346 1977
Fragments arranged by size:
2195 1977 1452 861 658 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2936 5531 2936
Size: 2595 5761
BtgI C'CryG_G
Cuts at: 513 1065 2343 2406 3321 3603 5138 5613 513
Size: 552 1278 63 915 282 1535 475 3256
Fragments arranged by size:
3256 1535 1278 915 552 475 282 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2229 2691 3627 487
Size: 683 56 1003 462 936 5216
Fragments arranged by size:
5216 1003 936 683 462 56
BtsI GCAGTG_nn'
Cuts at: 2566 3253 3532 3785 5175 5590 5872 6666 6686
Size: 687 279 253 1390 415 282 794 20
Cuts at: 6686 2566
Size: 4236
Fragments arranged by size:
4236 1390 794 687 415 282 279 253
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2289 2324 4600 4635 529
Size: 35 1725 35 2276 35 4250
Fragments arranged by size:
4250 2276 1725 35 35 35
DraI TTT'AAA
Cuts at: 5660 5660
Size: 8356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7363 809
Size: 507 6047 1802
Fragments arranged by size:
6047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3309 3678 3751
Size: 54 1425 50 275 18 1479 369 73
Cuts at: 3751 3820 3913 4725 5453 5803 6692 8321 8
Size: 69 93 812 728 350 889 1629 43
Fragments arranged by size:
1629 1479 1425 889 812 728 369 350
275 93 73 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5803 1537
Size: 275 3991 4090
Fragments arranged by size:
4090 3991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 3560 4142 4268 5285 5333 6286 1353
Size: 1373 834 582 126 1017 48 953 3423
Fragments arranged by size:
3423 1373 1017 953 834 582 126 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2538 2641 2720 2788 3461 4414
Size: 1064 138 949 103 79 68 673 953
Cuts at: 4414 4574 4852 6943 7317 7463 387
Size: 160 278 2091 374 146 1280
Fragments arranged by size:
2091 1280 1064 953 949 673 374 278
160 146 138 103 79 68
EcoICRI GAG'CTC
Cuts at: 4059 5502 4059
Size: 1443 6913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2042 2465 2988 3091 3378 3392
Size: 393 396 14 423 523 103 287 14
Cuts at: 3392 4083 4292 4559 5094 5122 5314 5362 5584
Size: 691 209 267 535 28 192 48 222
Cuts at: 5584 6419 7016 7803 8071 1239
Size: 835 597 787 268 1524
Fragments arranged by size:
1524 835 787 691 597 535 523 423
396 393 287 268 267 222 209 192
103 48 28 14 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2601 3333 4818 2601
Size: 732 1485 6139
Fragments arranged by size:
6139 1485 732
EcoO109I rG'GnC_Cy
Cuts at: 2484 2857 3181 3340 3534 3958 3985 3986 4096
Size: 373 324 159 194 424 27 1 110
Cuts at: 4096 4258 4313 4468 4606 4607 4630 4714 5215
Size: 162 55 155 138 1 23 84 501
Cuts at: 5215 5216 5227 5359 5360 2484
Size: 1 11 132 1 5480
Fragments arranged by size:
5480 501 424 373 324 194 162 159
155 138 132 110 84 55 27 23
11 1 1 1 1
EcoRI G'AATT_C
Cuts at: 5665 6043 5665
Size: 378 7978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 8356
FspI TGC'GCA
Cuts at: 6862 6862
Size: 8356
HaeII r_GCGC'y
Cuts at: 1678 2157 3045 3051 3221 4042 5001 7503 1678
Size: 479 888 6 170 821 959 2502 2531
Fragments arranged by size:
2531 2502 959 888 821 479 170 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2633 2665 3584
Size: 32 135 32 557 32 472 32 919
Cuts at: 3584 3616 5192 5224 5532 5564 6997 7029 7071
Size: 32 1576 32 308 32 1433 32 42
Cuts at: 7071 7103 1373
Size: 32 2626
Fragments arranged by size:
2626 1576 1433 919 557 472 308 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5551 5700 5946 669
Size: 988 281 3613 149 246 3079
Fragments arranged by size:
3613 3079 988 281 246 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8356
HpaI GTT'AAC
Cuts at: 5946 5946
Size: 8356
KasI G'GCGC_C
Cuts at: 4038 4997 4038
Size: 959 7397
KpnI G_GTAC'C
Cuts at: 5681 5681
Size: 8356
MfeI C'AATT_G
Cuts at: 5955 5955
Size: 8356
MluI A'CGCG_T
Cuts at: 8232 8232
Size: 8356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3126 3223 7470 7654 1305
Size: 1821 97 4247 184 2007
Fragments arranged by size:
4247 2007 1821 184 97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3311 3680 3822 5455 10
Size: 54 1425 343 1479 369 142 1633 2911
Fragments arranged by size:
2911 1633 1479 1425 369 343 142 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 4001 6314 6673 6832 518
Size: 648 1393 1442 2313 359 159 2042
Fragments arranged by size:
2313 2042 1442 1393 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3142 3151 4225 4772 4851 4960 4968 4979
Size: 1536 9 1074 547 79 109 8 11
Cuts at: 4979 5263 5772 6449 7597 7842 1606
Size: 284 509 677 1148 245 2120
Fragments arranged by size:
2120 1536 1148 1074 677 547 509 284
245 109 79 11 9 8
NaeI GCC'GGC
Cuts at: 2271 2479 2985 3510 4668 2271
Size: 208 506 525 1158 5959
Fragments arranged by size:
5959 1158 525 506 208
NarI GG'CG_CC
Cuts at: 4039 4998 4039
Size: 959 7397
NcoI C'CATG_G
Cuts at: 513 1065 2343 3321 513
Size: 552 1278 978 5548
Fragments arranged by size:
5548 1278 978 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8356
NgoMIV G'CCGG_C
Cuts at: 2269 2477 2983 3508 4666 2269
Size: 208 506 525 1158 5959
Fragments arranged by size:
5959 1158 525 506 208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8356
NotI GC'GGCC_GC
Cuts at: 5803 5803
Size: 8356
NspI r_CATG'y
Cuts at: 1132 1229 4006 5714 7259 1132
Size: 97 2777 1708 1545 2229
Fragments arranged by size:
2777 2229 1708 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3709 3961 4164 4455 4465 1348
Size: 2361 252 203 291 10 5239
Fragments arranged by size:
5239 2361 291 252 203 10
PciI A'CATG_T
Cuts at: 7255 7255
Size: 8356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4679 5139 1108
Size: 3571 460 4325
Fragments arranged by size:
4325 3571 460
PfoI T'CCnGG_A
Cuts at: 2433 3410 3483 2433
Size: 977 73 7306
Fragments arranged by size:
7306 977 73
PmeI GTTT'AAAC
Cuts at: 5660 5660
Size: 8356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6425 6457 7938 7970 6425
Size: 32 1481 32 6811
Fragments arranged by size:
6811 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3181 3534 4096 4313 4714 3181
Size: 353 562 217 401 6823
Fragments arranged by size:
6823 562 401 353 217
PshAI GACnn'nnGTC
Cuts at: 1613 3330 1613
Size: 1717 6639
PsiI TTA'TAA
Cuts at: 5926 5926
Size: 8356
PspOMI G'GGCC_C
Cuts at: 2484 3985 4258 4468 4606 4630 5215 5359 2484
Size: 1501 273 210 138 24 585 144 5481
Fragments arranged by size:
5481 1501 585 273 210 144 138 24
PstI C_TGCA'G
Cuts at: 830 2187 3156 5358 5708 830
Size: 1357 969 2202 350 3478
Fragments arranged by size:
3478 2202 1357 969 350
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8356
PvuII CAG'CTG
Cuts at: 3142 3151 4225 4772 4960 4968 3142
Size: 9 1074 547 188 8 6530
Fragments arranged by size:
6530 1074 547 188 9 8
SacI G_AGCT'C
Cuts at: 4061 5504 4061
Size: 1443 6913
SalI G'TCGA_C
Cuts at: 1936 5698 1936
Size: 3762 4594
SanDI GG'GwC_CC
Cuts at: 3534 4714 3534
Size: 1180 7176
SapI GCTCTTCn'nnn_
Cuts at: 4268 5285 4268
Size: 1017 7339
SbfI CC_TGCA'GG
Cuts at: 5708 5708
Size: 8356
ScaI AGT'ACT
Cuts at: 6604 8316 6604
Size: 1712 6644
SfcI C'TryA_G
Cuts at: 826 1045 2095 2183 2361 3152 3516 5354 5704
Size: 219 1050 88 178 791 364 1838 350
Cuts at: 5704 6839 7520 7711 826
Size: 1135 681 191 1471
Fragments arranged by size:
1838 1471 1135 1050 791 681 364 350
219 191 178 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 2483 2483
Size: 8356
SfoI GGC'GCC
Cuts at: 4040 4999 4040
Size: 959 7397
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8356
SmaI CCC'GGG
Cuts at: 1872 2159 2473 5055 5225 5683 1872
Size: 287 314 2582 170 458 4545
Fragments arranged by size:
4545 2582 458 314 287 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4142 6460 7361 7623 820
Size: 197 926 2199 2318 901 262 1553
Fragments arranged by size:
2318 2199 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5653 6147 493
Size: 5160 494 2702
Fragments arranged by size:
5160 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8356
SphI G_CATG'C
Cuts at: 1132 5714 1132
Size: 4582 3774
SspI AAT'ATT
Cuts at: 5 52 6280 5
Size: 47 6228 2081
Fragments arranged by size:
6228 2081 47
StuI AGG'CCT
Cuts at: 1517 2075 1517
Size: 558 7798
StyI C'CwwG_G
Cuts at: 513 1065 2343 2943 3321 4569 4866 5577 5795
Size: 552 1278 600 378 1248 297 711 218
Cuts at: 5795 513
Size: 3074
Fragments arranged by size:
3074 1278 1248 711 600 552 378 297
218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3304 5131 5288 6383 6400 6553 6738
Size: 1255 1724 1827 157 1095 17 153 185
Cuts at: 6738 7183 325
Size: 445 1498
Fragments arranged by size:
1827 1724 1498 1255 1095 445 185 157
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6602 8314 96
Size: 274 80 33 51 1443 4625 1712 138
Fragments arranged by size:
4625 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8316 8348 8316
Size: 32 8324
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8356
XbaI T'CTAG_A
Cuts at: 5692 5692
Size: 8356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2756 3319 3958 2756
Size: 563 639 7154
Fragments arranged by size:
7154 639 563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8356
XmaI C'CCGG_G
Cuts at: 1870 2157 2471 5053 5223 5681 1870
Size: 287 314 2582 170 458 4545
Fragments arranged by size:
4545 2582 458 314 287 170
XmnI GAAnn'nnTTC
Cuts at: 2718 4448 4734 6485 2718
Size: 1730 286 1751 4589
Fragments arranged by size:
4589 1751 1730 286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6164 276
Size: 53 83 186 936 4630 2468
Fragments arranged by size:
4630 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI
SrfI SwaI