Restriction map for
pFN21ASDA0644
(Circular) (Six-base) MAPSORT of: pFN21ASDA0644.seq Check: 5226 from: 1 to: 7162
pFN21ASDA0644 7162 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2784 3358 2784
Size: 574 6588
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4972 278
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3933 4101 4505 1937
Size: 1996 168 404 4594
Fragments arranged by size:
4594 1996 404 168
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 5289 5662 1563
Size: 3726 373 3063
Fragments arranged by size:
3726 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3152 5225 6609 6877 1632
Size: 1520 2073 1384 268 1917
Fragments arranged by size:
2073 1917 1520 1384 268
AfeI AGC'GCT
Cuts at: 2111 3137 3463 2111
Size: 1026 326 5810
Fragments arranged by size:
5810 1026 326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6965
AflIII A'CryG_T
Cuts at: 1203 4242 6061 7038 1203
Size: 3039 1819 977 1327
Fragments arranged by size:
3039 1819 1327 977
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 5891 5891
Size: 7162
AleI CACnn'nnGTG
Cuts at: 1166 4283 4331 1166
Size: 3117 48 3997
Fragments arranged by size:
3997 3117 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4464 1184
Size: 3280 3882
AlwNI CAG_nnn'CTG
Cuts at: 2776 3247 4114 4905 6477 2776
Size: 471 867 791 1572 3461
Fragments arranged by size:
3461 1572 867 791 471
ApaI G_GGCC'C
Cuts at: 3488 3533 4225 3488
Size: 45 692 6425
Fragments arranged by size:
6425 692 45
ApaLI G'TGCA_C
Cuts at: 2143 5219 6375 2143
Size: 3076 1156 2930
Fragments arranged by size:
3076 2930 1156
ApoI r'AATT_y
Cuts at: 1493 4364 4471 4697 4849 6958 1493
Size: 2871 107 226 152 2109 1697
Fragments arranged by size:
2871 2109 1697 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 5716 160
Size: 872 4684 1606
Fragments arranged by size:
4684 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 2392 2753 3487 3585 4044 4300 4487
Size: 73 449 361 734 98 459 256 187
Cuts at: 4487 1870
Size: 4545
Fragments arranged by size:
4545 734 459 449 361 256 187 98
73
BamHI G'GATC_C
Cuts at: 2592 3257 3268 4492 4859 2592
Size: 665 11 1224 367 4895
Fragments arranged by size:
4895 1224 665 367 11
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4277 4483 5938 618
Size: 325 217 627 2490 206 1455 1842
Fragments arranged by size:
2490 1842 1455 627 325 217 206
BanII G_rGCy'C
Cuts at: 1391 2097 2160 2195 3012 3488 3533 3612 4225
Size: 706 63 35 817 476 45 79 613
Cuts at: 4225 4481 1391
Size: 256 4072
Fragments arranged by size:
4072 817 706 613 476 256 79 63
45 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2536 3172 928
Size: 971 637 636 4918
Fragments arranged by size:
4918 971 637 636
BbvCI CC'TCA_GC
Cuts at: 3280 3280
Size: 7162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5353 5387 1903
Size: 34 3416 34 3678
Fragments arranged by size:
3678 3416 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5054 6264 5054
Size: 1210 5952
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2223 2525 2994
Size: 107 122 71 631 605 551 302 469
Cuts at: 2994 4095 4166 5773 136
Size: 1101 71 1607 1525
Fragments arranged by size:
1607 1525 1101 631 605 551 469 302
122 107 71 71
BglII A'GATC_T
Cuts at: 7157 7157
Size: 7162
BlpI GC'TnA_GC
Cuts at: 2888 3011 3032 4579 2888
Size: 123 21 1547 5471
Fragments arranged by size:
5471 1547 123 21
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2147 2172 2399 3488 3533 3692 4225
Size: 315 42 25 227 1089 45 159 533
Cuts at: 4225 4280 5223 6379 1790
Size: 55 943 1156 2573
Fragments arranged by size:
2573 1156 1089 943 533 315 227 159
55 45 42 25
BmgBI CAC'GTC
Cuts at: 1318 2203 2783 1318
Size: 885 580 5697
Fragments arranged by size:
5697 885 580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3709 4893 5851
Size: 416 53 83 399 24 2292 1184 958
Cuts at: 5851 442
Size: 1753
Fragments arranged by size:
2292 1753 1184 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 7162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2600 2894 2933 2951 3374 3479 5822
Size: 1265 96 294 39 18 423 105 2343
Cuts at: 5822 1239
Size: 2579
Fragments arranged by size:
2579 2343 1265 423 294 105 96 39
18
Bpu10I CC'TnA_GC
Cuts at: 2459 2801 2945 3280 2459
Size: 342 144 335 6341
Fragments arranged by size:
6341 342 335 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5287 6152 6450 5287
Size: 865 298 5999
Fragments arranged by size:
5999 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3362 5825 882
Size: 617 1863 2463 2219
Fragments arranged by size:
2463 2219 1863 617
BsaAI yAC'GTr
Cuts at: 493 1204 4459 4953 493
Size: 711 3255 494 2702
Fragments arranged by size:
3255 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7156 7156
Size: 7162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3344 4098 4175 4969
Size: 53 83 186 936 1811 754 77 794
Cuts at: 4969 5351 5986 275
Size: 382 635 1451
Fragments arranged by size:
1811 1451 936 794 754 635 382 186
83 77 53
BsaWI w'CCGG_w
Cuts at: 2589 4075 5595 5993 6267 6414 6825 2589
Size: 1486 1520 398 274 147 411 2926
Fragments arranged by size:
2926 1520 1486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3610 3640 4232 4262 1178
Size: 30 165 30 2207 30 592 30 4078
Fragments arranged by size:
4078 2207 592 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2707 3161 3350 3380 1186
Size: 1521 454 189 30 4968
Fragments arranged by size:
4968 1521 454 189 30
BseYI C'CCAG_C
Cuts at: 2130 2622 3067 3413 3503 3567 3831 3865 6365
Size: 492 445 346 90 64 264 34 2500
Cuts at: 6365 6861 2130
Size: 496 2431
Fragments arranged by size:
2500 2431 496 492 445 346 264 90
64 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 2906 2954 3206 3539 1163
Size: 573 564 606 48 252 333 4786
Fragments arranged by size:
4786 606 573 564 333 252 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3327 3863 4378 4612 5373 6401
Size: 275 186 1326 536 515 234 761 1028
Cuts at: 6401 1540
Size: 2301
Fragments arranged by size:
2301 1326 1028 761 536 515 275 234
186
BsiHKAI G_wGCw'C
Cuts at: 2147 2208 3012 3699 3849 4155 5223 5308 6379
Size: 61 804 687 150 306 1068 85 1071
Cuts at: 6379 2147
Size: 2930
Fragments arranged by size:
2930 1071 1068 804 687 306 150 85
61
BsmI GAATG_Cn'
Cuts at: 4673 4766 4673
Size: 93 7069
BsmBI CGTCTCn'nnnn_
Cuts at: 2789 4182 4414 4439 2789
Size: 1393 232 25 5512
Fragments arranged by size:
5512 1393 232 25
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2105 2147 2160 2172 2195 2208
Size: 399 307 8 42 13 12 23 13
Cuts at: 2208 2399 3012 3488 3533 3612 3692 3699 3849
Size: 191 613 476 45 79 80 7 150
Cuts at: 3849 4155 4225 4280 4481 5223 5308 6379 1391
Size: 306 70 55 201 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 476 399 307 306
201 191 150 85 80 79 70 55
45 42 23 13 13 12 8 7
BspEI T'CCGG_A
Cuts at: 6825 6825
Size: 7162
BspHI T'CATG_A
Cuts at: 5051 6781 5051
Size: 1730 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2571 2784 3116 3344 3358 3561 3945
Size: 1454 273 213 332 228 14 203 384
Cuts at: 3945 4517 844
Size: 572 3489
Fragments arranged by size:
3489 1454 572 384 332 273 228 213
203 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2310 2949 5049 1394
Size: 429 487 639 2100 3507
Fragments arranged by size:
3507 2100 639 487 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2959 5657 5831 65
Size: 1183 1711 2698 174 1396
Fragments arranged by size:
2698 1711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2997 3117 3165 3650 4280 4317 5806
Size: 110 1187 120 48 485 630 37 1489
Cuts at: 5806 5993 1700
Size: 187 2869
Fragments arranged by size:
2869 1489 1187 630 485 187 120 110
48 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7162
BssHII G'CGCG_C
Cuts at: 1925 3667 3873 3875 4338 1925
Size: 1742 206 2 463 4749
Fragments arranged by size:
4749 1742 463 206 2
BssSI C'ACGA_G
Cuts at: 2054 5222 6234 2054
Size: 3168 1012 2982
Fragments arranged by size:
3168 2982 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7162
BstBI TT'CG_AA
Cuts at: 2007 5966 2007
Size: 3959 3203
BstEII G'GTnAC_C
Cuts at: 1184 4066 1184
Size: 2882 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 3445 3445
Size: 7162
BstYI r'GATC_y
Cuts at: 1972 2304 2592 3085 3257 3268 3592 4492 4859
Size: 332 288 493 172 11 324 900 367
Cuts at: 4859 5244 5261 6713 6799 6811 7157 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 900 493 385 367 346 332
324 288 172 86 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2832 2832
Size: 7162
BtgI C'CryG_G
Cuts at: 513 1065 2022 2464 3321 3525 4272 513
Size: 552 957 442 857 204 747 3403
Fragments arranged by size:
3403 957 857 747 552 442 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3627 487
Size: 683 56 2401 4022
Fragments arranged by size:
4022 2401 683 56
BtsI GCAGTG_nn'
Cuts at: 3194 4051 4678 5472 5492 3194
Size: 857 627 794 20 4864
Fragments arranged by size:
4864 857 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7127
DraI TTT'AAA
Cuts at: 4466 4466
Size: 7162
DraIII CAC_nnn'GTG
Cuts at: 2203 4335 2203
Size: 2132 5030
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6169 809
Size: 507 4853 1802
Fragments arranged by size:
4853 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2688 2733
Size: 54 1425 50 275 18 258 600 45
Cuts at: 2733 2976 2986 3324 3860 3904 3957 4319 4609
Size: 243 10 338 536 44 53 362 290
Cuts at: 4609 5498 7127 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 600 536 362 338 290
275 258 243 54 53 50 45 44
43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 3324 3860 4609 1537
Size: 275 1512 536 749 4090
Fragments arranged by size:
4090 1512 749 536 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 5092 1353
Size: 993 2746 3423
Fragments arranged by size:
3423 2746 993
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2953 3872 4225 4279 4367 5749
Size: 1064 138 1364 919 353 54 88 1382
Cuts at: 5749 6123 6269 387
Size: 374 146 1280
Fragments arranged by size:
1382 1364 1280 1064 919 374 353 146
138 88 54
EcoICRI GAG'CTC
Cuts at: 3010 3010
Size: 7162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 2600 2894 2933 2951 3152 3374
Size: 393 872 96 294 39 18 201 222
Cuts at: 3374 3479 5225 5822 6609 6877 1239
Size: 105 1746 597 787 268 1524
Fragments arranged by size:
1746 1524 872 787 597 393 294 268
222 201 105 96 39 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 4076 2378
Size: 1698 5464
EcoO109I rG'GnC_Cy
Cuts at: 2479 2854 3484 4221 4222 2479
Size: 375 630 737 1 5419
Fragments arranged by size:
5419 737 630 375 1
EcoRI G'AATT_C
Cuts at: 4364 4471 4849 4364
Size: 107 378 6677
Fragments arranged by size:
6677 378 107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2656 2632
Size: 24 7138
FspI TGC'GCA
Cuts at: 2414 2423 3020 5668 2414
Size: 9 597 2648 3908
Fragments arranged by size:
3908 2648 597 9
HaeII r_GCGC'y
Cuts at: 1678 2113 2387 2531 2939 3039 3139 3465 3822
Size: 435 274 144 408 100 100 326 357
Cuts at: 3822 6309 1678
Size: 2487 2531
Fragments arranged by size:
2531 2487 435 408 357 326 274 144
100 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 3140 3172 3664
Size: 32 135 32 724 32 812 32 492
Cuts at: 3664 3696 3806 3838 5803 5835 5877 5909 1373
Size: 32 110 32 1965 32 42 32 2626
Fragments arranged by size:
2626 1965 812 724 492 135 110 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4102 4138 4506 4752 669
Size: 988 281 2164 36 368 246 3079
Fragments arranged by size:
3079 2164 988 368 281 246 36
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7162
HpaI GTT'AAC
Cuts at: 4752 4752
Size: 7162
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 4761 4761
Size: 7162
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 3605 3744 3777 6276 6460 1305
Size: 1305 995 139 33 2499 184 2007
Fragments arranged by size:
2499 2007 1305 995 184 139 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3906 10
Size: 54 1425 343 2074 3266
Fragments arranged by size:
3266 2074 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4283 4331 5120 5479 5638 518
Size: 648 3117 48 789 359 159 2042
Fragments arranged by size:
3117 2042 789 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2086 2410 2466 2704 2896 3247 3296 3527
Size: 480 324 56 238 192 351 49 231
Cuts at: 3527 3571 3745 3865 4111 4578 5255 6403 6648
Size: 44 174 120 246 467 677 1148 245
Cuts at: 6648 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 480 467 351 324 246
245 238 231 192 174 120 56 49
44
NaeI GCC'GGC
Cuts at: 2999 3119 3167 3652 2999
Size: 120 48 485 6509
Fragments arranged by size:
6509 485 120 48
NcoI C'CATG_G
Cuts at: 513 1065 2022 4272 513
Size: 552 957 2250 3403
Fragments arranged by size:
3403 2250 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7162
NgoMIV G'CCGG_C
Cuts at: 2997 3117 3165 3650 2997
Size: 120 48 485 6509
Fragments arranged by size:
6509 485 120 48
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 4246 4520 6065 1132
Size: 97 3017 274 1545 2229
Fragments arranged by size:
3017 2229 1545 274 97
PasI CC'CwG_GG
Cuts at: 1348 2521 1348
Size: 1173 5989
PciI A'CATG_T
Cuts at: 4242 6061 4242
Size: 1819 5343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2266 1108
Size: 1158 6004
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 6744 6776 5231
Size: 32 1481 32 5617
Fragments arranged by size:
5617 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 4732 4732
Size: 7162
PspOMI G'GGCC_C
Cuts at: 3484 3529 4221 3484
Size: 45 692 6425
Fragments arranged by size:
6425 692 45
PstI C_TGCA'G
Cuts at: 830 2295 2748 3355 4443 4514 830
Size: 1465 453 607 1088 71 3478
Fragments arranged by size:
3478 1465 1088 607 453 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
PvuII CAG'CTG
Cuts at: 2896 3247 4111 2896
Size: 351 864 5947
Fragments arranged by size:
5947 864 351
SacI G_AGCT'C
Cuts at: 3012 3012
Size: 7162
SacII CC_GC'GG
Cuts at: 2467 3528 2467
Size: 1061 6101
SalI G'TCGA_C
Cuts at: 1936 4100 4504 1936
Size: 2164 404 4594
Fragments arranged by size:
4594 2164 404
SapI GCTCTTCn'nnn_
Cuts at: 2346 2346
Size: 7162
SbfI CC_TGCA'GG
Cuts at: 3355 4514 3355
Size: 1159 6003
ScaI AGT'ACT
Cuts at: 5410 7122 5410
Size: 1712 5450
SexAI A'CCwGG_T
Cuts at: 3993 3993
Size: 7162
SfcI C'TryA_G
Cuts at: 826 1045 2212 2291 2666 2744 3351 4439 4510
Size: 219 1167 79 375 78 607 1088 71
Cuts at: 4510 5645 6326 6517 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 1088 681 607 375 219
191 79 78 71
SfiI GGCCn_nnn'nGGCC
Cuts at: 2994 4166 2994
Size: 1172 5990
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 3650 4280 1700
Size: 1950 630 4582
Fragments arranged by size:
4582 1950 630
SmaI CCC'GGG
Cuts at: 1872 2394 3489 4046 4489 1872
Size: 522 1095 557 443 4545
Fragments arranged by size:
4545 1095 557 522 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5266 6167 6429 820
Size: 197 926 3323 901 262 1553
Fragments arranged by size:
3323 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4459 4953 493
Size: 3966 494 2702
Fragments arranged by size:
3966 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7162
SphI G_CATG'C
Cuts at: 1132 4520 1132
Size: 3388 3774
SrfI GCCC'GGGC
Cuts at: 3489 3489
Size: 7162
SspI AAT'ATT
Cuts at: 5 52 5086 5
Size: 47 5034 2081
Fragments arranged by size:
5034 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 3129 1517
Size: 1320 292 5550
Fragments arranged by size:
5550 1320 292
StyI C'CwwG_G
Cuts at: 513 1065 2022 2553 4119 4161 4272 4601 513
Size: 552 957 531 1566 42 111 329 3074
Fragments arranged by size:
3074 1566 957 552 531 329 111 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5189 5206 5359 5544 5989 325
Size: 1255 3609 17 153 185 445 1498
Fragments arranged by size:
3609 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 4245 5408
Size: 274 80 33 51 1443 318 1950 1163
Cuts at: 5408 7120 96
Size: 1712 138
Fragments arranged by size:
1950 1712 1443 1163 318 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4232 4264 7122 7154 4232
Size: 32 2858 32 4240
Fragments arranged by size:
4240 2858 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2540 1316
Size: 1224 5938
XbaI T'CTAG_A
Cuts at: 3088 4498 3088
Size: 1410 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3722 3722
Size: 7162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7162
XmaI C'CCGG_G
Cuts at: 1870 2392 3487 4044 4487 1870
Size: 522 1095 557 443 4545
Fragments arranged by size:
4545 1095 557 522 443
XmnI GAAnn'nnTTC
Cuts at: 2615 5291 2615
Size: 2676 4486
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4970 276
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI
SwaI