Restriction map for
pFN21ASDA0632
(Circular) (Six-base) MAPSORT of: pFN21ASDA0632.seq Check: 7571 from: 1 to: 6829
pFN21ASDA0632 6829 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3736 4172 1937
Size: 1799 436 4594
Fragments arranged by size:
4594 1799 436
Acc65I G'GTAC_C
Cuts at: 3942 4150 3942
Size: 208 6621
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2143 2534 2609 2738 2804 2860 3068 3077
Size: 511 391 75 129 66 56 208 9
Cuts at: 3077 3335 4892 6276 6544 1632
Size: 258 1557 1384 268 1917
Fragments arranged by size:
1917 1557 1384 511 391 268 258 208
129 75 66 56 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 5728 6705 1203
Size: 4525 977 1327
Fragments arranged by size:
4525 1327 977
AgeI A'CCGG_T
Cuts at: 3939 5660 3939
Size: 1721 5108
AhdI GACnn_n'nnGTC
Cuts at: 5558 5558
Size: 6829
AleI CACnn'nnGTG
Cuts at: 1166 3305 3599 1166
Size: 2139 294 4396
Fragments arranged by size:
4396 2139 294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6829
AlwNI CAG_nnn'CTG
Cuts at: 2512 2734 4006 4572 6144 2512
Size: 222 1272 566 1572 3197
Fragments arranged by size:
3197 1572 1272 566 222
ApaI G_GGCC'C
Cuts at: 2062 2062
Size: 6829
ApaLI G'TGCA_C
Cuts at: 4886 6042 4886
Size: 1156 5673
ApoI r'AATT_y
Cuts at: 1493 4138 4364 4516 6625 1493
Size: 2645 226 152 2109 1697
Fragments arranged by size:
2645 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5383 160
Size: 872 4351 1606
Fragments arranged by size:
4351 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 2230 3009 3356 4154 1870
Size: 73 287 779 347 798 4545
Fragments arranged by size:
4545 798 779 347 287 73
AvrII C'CTAG_G
Cuts at: 3022 3022
Size: 6829
BamHI G'GATC_C
Cuts at: 4159 4526 4159
Size: 367 6462
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 3714 3942 4150 5605
Size: 325 217 627 365 1562 228 208 1455
Cuts at: 5605 618
Size: 1842
Fragments arranged by size:
1842 1562 1455 627 365 325 228 217
208
BanII G_rGCy'C
Cuts at: 1391 2062 2225 2400 2640 2757 3282 4148 1391
Size: 671 163 175 240 117 525 866 4072
Fragments arranged by size:
4072 866 671 525 240 175 163 117
BbeI G_GCGC'C
Cuts at: 2156 2156
Size: 6829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2271 2918 928
Size: 971 372 647 4839
Fragments arranged by size:
4839 971 647 372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5020 5054 1903
Size: 34 3083 34 3678
Fragments arranged by size:
3678 3083 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3259 4721 5931 3259
Size: 1462 1210 4157
Fragments arranged by size:
4157 1462 1210
BclI T'GATC_A
Cuts at: 1548 2460 1548
Size: 912 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 3438 5440
Size: 107 122 71 631 605 442 1324 2002
Cuts at: 5440 136
Size: 1525
Fragments arranged by size:
2002 1525 1324 631 605 442 122 107
71
BglII A'GATC_T
Cuts at: 6824 6824
Size: 6829
BlpI GC'TnA_GC
Cuts at: 4246 4246
Size: 6829
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2856 3243 3799 4111 4890 6046 1790
Size: 272 794 387 556 312 779 1156 2573
Fragments arranged by size:
2573 1156 794 779 556 387 312 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 3086 3913
Size: 416 53 83 399 24 921 748 827
Cuts at: 3913 4560 5518 442
Size: 647 958 1753
Fragments arranged by size:
1753 958 921 827 748 647 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2058 2058
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 2918 3194 3201 3455 3650 3926 3986
Size: 1415 264 276 7 254 195 276 60
Cuts at: 3986 5489 1239
Size: 1503 2579
Fragments arranged by size:
2579 1503 1415 276 276 264 254 195
60 7
Bpu10I CC'TnA_GC
Cuts at: 3446 3446
Size: 6829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4954 5819 6117 4954
Size: 865 298 5666
Fragments arranged by size:
5666 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2864 3385 3618 5492 882
Size: 617 1365 521 233 1874 2219
Fragments arranged by size:
2219 1874 1365 617 521 233
BsaAI yAC'GTr
Cuts at: 493 1204 2857 4126 4620 493
Size: 711 1653 1269 494 2702
Fragments arranged by size:
2702 1653 1269 711 494
BsaBI GATnn'nnATC
Cuts at: 6823 6823
Size: 6829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2153 2646 4636 5018
Size: 53 83 186 936 620 493 1990 382
Cuts at: 5018 5653 275
Size: 635 1451
Fragments arranged by size:
1990 1451 936 635 620 493 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2392 2922 3226 3939 4053 5262 5660 5934 6081
Size: 530 304 713 114 1209 398 274 147
Cuts at: 6081 6492 2392
Size: 411 2729
Fragments arranged by size:
2729 1209 713 530 411 398 304 274
147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 2294 2324 1178
Size: 30 165 30 825 30 36 30 5683
Fragments arranged by size:
5683 825 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 2161 2285 2288 2291 2294 2302 2534
Size: 972 3 124 3 3 3 8 232
Cuts at: 2534 2882 3083 1186
Size: 348 201 4932
Fragments arranged by size:
4932 972 348 232 201 124 8 3
3 3 3
BseYI C'CCAG_C
Cuts at: 3281 3403 3619 6032 6528 3281
Size: 122 216 2413 496 3582
Fragments arranged by size:
3582 2413 496 216 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6256
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4279 5040 6068 1540
Size: 275 186 2278 761 1028 2301
Fragments arranged by size:
2301 2278 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2757 3465 3564 4890 4975 6046 2757
Size: 708 99 1326 85 1071 3540
Fragments arranged by size:
3540 1326 1071 708 99 85
BsmI GAATG_Cn'
Cuts at: 4340 4433 4340
Size: 93 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2225 2400 2640 2757 2856 3243
Size: 399 272 163 175 240 117 99 387
Cuts at: 3243 3282 3465 3564 3799 4111 4148 4890 4975
Size: 39 183 99 235 312 37 742 85
Cuts at: 4975 6046 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 312 272 240
235 183 175 163 117 99 99 85
39 37
BspEI T'CCGG_A
Cuts at: 3226 4053 6492 3226
Size: 827 2439 3563
Fragments arranged by size:
3563 2439 827
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 3000 3693 4110 4184 844
Size: 1520 636 693 417 74 3489
Fragments arranged by size:
3489 1520 693 636 417 74
BsrBI CCG'CTC
Cuts at: 1394 1823 4716 1394
Size: 429 2893 3507
Fragments arranged by size:
3507 2893 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5324 5498 65
Size: 1183 4076 174 1396
Fragments arranged by size:
4076 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3411 3652 3939 4082 5473 5660 1700
Size: 110 1601 241 287 143 1391 187 2869
Fragments arranged by size:
2869 1601 1391 287 241 187 143 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6829
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 4889 5901 4889
Size: 1012 5817
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2734 794
Size: 1940 4889
BstBI TT'CG_AA
Cuts at: 2007 5633 2007
Size: 3626 3203
BstEII G'GTnAC_C
Cuts at: 1184 3394 3769 1184
Size: 2210 375 4244
Fragments arranged by size:
4244 2210 375
BstYI r'GATC_y
Cuts at: 1972 4159 4526 4911 4928 6380 6466 6478 6824
Size: 2187 367 385 17 1452 86 12 346
Cuts at: 6824 1972
Size: 1977
Fragments arranged by size:
2187 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2810 2810
Size: 6829
BtgI C'CryG_G
Cuts at: 513 1065 2022 2067 2247 2535 3327 513
Size: 552 957 45 180 288 792 4015
Fragments arranged by size:
4015 957 792 552 288 180 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6090
Fragments arranged by size:
6090 683 56
BtsI GCAGTG_nn'
Cuts at: 2453 3312 4007 4345 5139 5159 2453
Size: 859 695 338 794 20 4123
Fragments arranged by size:
4123 859 794 695 338 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6829
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6794
DraI TTT'AAA
Cuts at: 4133 4133
Size: 6829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5836 809
Size: 507 4520 1802
Fragments arranged by size:
4520 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 2188 2250
Size: 54 1425 50 275 18 311 47 62
Cuts at: 2250 3107 3501 3705 3810 4276 5165 6794 8
Size: 857 394 204 105 466 889 1629 43
Fragments arranged by size:
1629 1425 889 857 466 394 311 275
204 105 62 54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4276 1537
Size: 275 2464 4090
Fragments arranged by size:
4090 2464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3605 4759 1353
Size: 2252 1154 3423
Fragments arranged by size:
3423 2252 1154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3887 5416 5790 5936 387
Size: 1064 138 2298 1529 374 146 1280
Fragments arranged by size:
2298 1529 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2755 2755
Size: 6829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2534 2609 2654 2738 2804 2860
Size: 393 511 391 75 45 84 66 56
Cuts at: 2860 2918 3068 3077 3194 3201 3335 3455 3650
Size: 58 150 9 117 7 134 120 195
Cuts at: 3650 3926 3986 4892 5489 6276 6544 1239
Size: 276 60 906 597 787 268 1524
Fragments arranged by size:
1524 906 787 597 511 393 391 276
268 195 150 134 120 117 84 75
66 60 58 56 45 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2462 2591 2189
Size: 273 129 6427
Fragments arranged by size:
6427 273 129
EcoO109I rG'GnC_Cy
Cuts at: 2058 2292 2629 3028 3169 3430 3486 3670 3874
Size: 234 337 399 141 261 56 184 204
Cuts at: 3874 2058
Size: 5013
Fragments arranged by size:
5013 399 337 261 234 204 184 141
56
EcoRI G'AATT_C
Cuts at: 4138 4516 4138
Size: 378 6451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2935 2614
Size: 321 6508
FspI TGC'GCA
Cuts at: 2803 5335 2803
Size: 2532 4297
HaeII r_GCGC'y
Cuts at: 1678 2156 2715 3647 5976 1678
Size: 478 559 932 2329 2531
Fragments arranged by size:
2531 2329 932 559 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2294 2326 2941
Size: 32 135 32 492 32 198 32 615
Cuts at: 2941 2973 5470 5502 5544 5576 1373
Size: 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 615 492 198 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3343 4173 4419 669
Size: 988 281 1405 830 246 3079
Fragments arranged by size:
3079 1405 988 830 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6829
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KasI G'GCGC_C
Cuts at: 2152 2152
Size: 6829
KpnI G_GTAC'C
Cuts at: 3946 4154 3946
Size: 208 6621
MfeI C'AATT_G
Cuts at: 4428 4428
Size: 6829
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5943 6127 1305
Size: 960 3678 184 2007
Fragments arranged by size:
3678 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3707 10
Size: 54 1425 343 1875 3132
Fragments arranged by size:
3132 1875 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3305 3599 3752 4787 5146 5305 518
Size: 648 2139 294 153 1035 359 159 2042
Fragments arranged by size:
2139 2042 1035 648 359 294 159 153
MspA1I CmG'CkG
Cuts at: 1606 2179 2734 3106 3184 3407 3904 4245 4922
Size: 573 555 372 78 223 497 341 677
Cuts at: 4922 6070 6315 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 573 555 497 372 341
245 223 78
NarI GG'CG_CC
Cuts at: 2153 2153
Size: 6829
NcoI C'CATG_G
Cuts at: 513 1065 2022 2067 2535 513
Size: 552 957 45 468 4807
Fragments arranged by size:
4807 957 552 468 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 3164 4187 5732 1132
Size: 97 1935 1023 1545 2229
Fragments arranged by size:
2229 1935 1545 1023 97
PasI CC'CwG_GG
Cuts at: 1348 3063 4066 1348
Size: 1715 1003 4111
Fragments arranged by size:
4111 1715 1003
PciI A'CATG_T
Cuts at: 5728 5728
Size: 6829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2406 1108
Size: 1298 5531
PfoI T'CCnGG_A
Cuts at: 2315 3518 3552 2315
Size: 1203 34 5592
Fragments arranged by size:
5592 1203 34
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PmlI CAC'GTG
Cuts at: 2857 2857
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2292 2629 3028 3486 3874 2292
Size: 337 399 458 388 5247
Fragments arranged by size:
5247 458 399 388 337
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PspOMI G'GGCC_C
Cuts at: 2058 2058
Size: 6829
PstI C_TGCA'G
Cuts at: 830 2739 3532 3690 4181 830
Size: 1909 793 158 491 3478
Fragments arranged by size:
3478 1909 793 491 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
PvuII CAG'CTG
Cuts at: 2179 2734 3184 3407 3904 2179
Size: 555 450 223 497 5104
Fragments arranged by size:
5104 555 497 450 223
SacI G_AGCT'C
Cuts at: 2757 2757
Size: 6829
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SanDI GG'GwC_CC
Cuts at: 2292 3028 3874 2292
Size: 736 846 5247
Fragments arranged by size:
5247 846 736
SapI GCTCTTCn'nnn_
Cuts at: 3605 3605
Size: 6829
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 5077 6789 5077
Size: 1712 5117
SexAI A'CCwGG_T
Cuts at: 3564 3564
Size: 6829
SfcI C'TryA_G
Cuts at: 826 1045 2101 2705 2735 2966 3372 3528 3686
Size: 219 1056 604 30 231 406 156 158
Cuts at: 3686 3737 3790 4177 5312 5993 6184 826
Size: 51 53 387 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 681 604 406 387 231
219 191 158 156 53 51 30
SfoI GGC'GCC
Cuts at: 2154 2154
Size: 6829
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 3358 4156 1872
Size: 1486 798 4545
Fragments arranged by size:
4545 1486 798
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4933 5834 6096 820
Size: 197 926 2990 901 262 1553
Fragments arranged by size:
2990 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4126 4620 493
Size: 3633 494 2702
Fragments arranged by size:
3633 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6829
SphI G_CATG'C
Cuts at: 1132 4187 1132
Size: 3055 3774
SspI AAT'ATT
Cuts at: 5 52 4753 5
Size: 47 4701 2081
Fragments arranged by size:
4701 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 5749
StyI C'CwwG_G
Cuts at: 513 1065 2022 2067 2535 3022 3110 3129 3639
Size: 552 957 45 468 487 88 19 510
Cuts at: 3639 3946 4111 4268 513
Size: 307 165 157 3074
Fragments arranged by size:
3074 957 552 510 487 468 307 165
157 88 45 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4856 4873 5026 5211 5656 325
Size: 1255 3276 17 153 185 445 1498
Fragments arranged by size:
3276 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5075 6787 96
Size: 274 80 33 51 1443 3098 1712 138
Fragments arranged by size:
3098 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 6789 6821 2228
Size: 32 4529 32 2236
Fragments arranged by size:
4529 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3160
Size: 6829
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6829
XmaI C'CCGG_G
Cuts at: 1870 3356 4154 1870
Size: 1486 798 4545
Fragments arranged by size:
4545 1486 798
XmnI GAAnn'nnTTC
Cuts at: 2597 4958 2597
Size: 2361 4468
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII
SfiI SrfI SwaI