Restriction map for
pFN21ASDA0626
(Circular) (Six-base) MAPSORT of: pFN21ASDA0626.seq Check: 596 from: 1 to: 5956
pFN21ASDA0626 5956 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3233 3233
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2475 2880 3249 3299 1937
Size: 538 405 369 50 4594
Fragments arranged by size:
4594 538 405 369 50
Acc65I G'GTAC_C
Cuts at: 3154 3277 3154
Size: 123 5833
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2921 3153 4019 5403 5671 1632
Size: 1289 232 866 1384 268 1917
Fragments arranged by size:
1917 1384 1289 866 268 232
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 2391 2393 4855 5832 1203
Size: 1188 2 2462 977 1327
Fragments arranged by size:
2462 1327 1188 977 2
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 2141 2396 1166
Size: 975 255 4726
Fragments arranged by size:
4726 975 255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2827 3265 3491 3643 5752 1493
Size: 1334 438 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 438 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2349 2382 2349
Size: 33 5923
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 3154 3277 4732 618
Size: 325 217 627 630 737 123 1455 1842
Fragments arranged by size:
1842 1455 737 630 627 325 217 123
BanII G_rGCy'C
Cuts at: 1391 2156 3275 1391
Size: 765 1119 4072
Fragments arranged by size:
4072 1119 765
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2570 928
Size: 971 671 4314
Fragments arranged by size:
4314 971 671
BbvCI CC'TCA_GC
Cuts at: 2958 2958
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2714 2748 2900 2934 4147 4181 1903
Size: 34 777 34 152 34 1213 34 3678
Fragments arranged by size:
3678 1213 777 152 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2098 2377 3848 5058 2098
Size: 279 1471 1210 2996
Fragments arranged by size:
2996 1471 1210 279
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2163 4017 5173 1790
Size: 283 90 1854 1156 2573
Fragments arranged by size:
2573 1854 1156 283 90
BmgBI CAC'GTC
Cuts at: 1318 3114 1318
Size: 1796 4160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 3687 4645
Size: 416 53 83 399 24 1269 1001 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1269 1001 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2763 2859 2763
Size: 96 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2481 2829 2831 4616 1239
Size: 1242 348 2 1785 2579
Fragments arranged by size:
2579 1785 1242 348 2
Bpu10I CC'TnA_GC
Cuts at: 2958 2958
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2394 3253 3747 493
Size: 711 1190 859 494 2702
Fragments arranged by size:
2702 1190 859 711 494
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2934 3763 4145 4780
Size: 53 83 186 936 1401 829 382 635
Cuts at: 4780 275
Size: 1451
Fragments arranged by size:
1451 1401 936 829 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2328 2331 2801 2828 3068 1186
Size: 1142 3 470 27 240 4074
Fragments arranged by size:
4074 1142 470 240 27 3
BseYI C'CCAG_C
Cuts at: 5159 5655 5159
Size: 496 5460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2625 2930 1163
Size: 573 889 305 4189
Fragments arranged by size:
4189 889 573 305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4017 4102 5173 4017
Size: 85 1071 4800
Fragments arranged by size:
4800 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2156 2163 3275 4017 4102 5173
Size: 399 283 83 7 1112 742 85 1071
Cuts at: 5173 1391
Size: 2174
Fragments arranged by size:
2174 1112 1071 742 399 283 85 83
7
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3233 3311 844
Size: 2389 78 3489
Fragments arranged by size:
3489 2389 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 4451 4625 65
Size: 1183 921 2282 174 1396
Fragments arranged by size:
2282 1396 1183 921 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2948 4016 5028 2948
Size: 1068 1012 3876
Fragments arranged by size:
3876 1068 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 2007 4760 2007
Size: 2753 3203
BstEII G'GTnAC_C
Cuts at: 1184 2434 1184
Size: 1250 4706
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 3026 2515
Size: 511 5445
BstYI r'GATC_y
Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951
Size: 1314 367 385 17 1452 86 12 346
Cuts at: 5951 1972
Size: 1977
Fragments arranged by size:
1977 1452 1314 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2703 513
Size: 552 957 681 3766
Fragments arranged by size:
3766 957 681 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2925 487
Size: 683 56 1699 3518
Fragments arranged by size:
3518 1699 683 56
BtsI GCAGTG_nn'
Cuts at: 3210 3472 4266 4286 3210
Size: 262 794 20 4880
Fragments arranged by size:
4880 794 262 20
ClaI AT'CG_AT
Cuts at: 1554 2029 1554
Size: 475 5481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 2130 3260 2130
Size: 1130 4826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 3064 4963 809
Size: 507 1155 593 1899 1802
Fragments arranged by size:
1899 1802 1155 593 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921
Size: 54 1425 50 275 18 1573 889 1629
Cuts at: 5921 8
Size: 43
Fragments arranged by size:
1629 1573 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2289 2721 3129 3886 1353
Size: 677 259 432 408 757 3423
Fragments arranged by size:
3423 757 677 432 408 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2650 3086 4543 4917 5063 387
Size: 1064 138 1061 436 1457 374 146 1280
Fragments arranged by size:
1457 1280 1064 1061 436 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2481 2829 2831 2921 3153 4019 4616
Size: 393 849 348 2 90 232 866 597
Cuts at: 4616 5403 5671 1239
Size: 787 268 1524
Fragments arranged by size:
1524 866 849 787 597 393 348 268
232 90 2
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2539 2571 2924 2956 3098
Size: 32 135 32 967 32 353 32 142
Cuts at: 3098 3113 3130 3145 4597 4629 4671 4703 1373
Size: 15 17 15 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 967 353 142 135 42 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3158 3281 3158
Size: 123 5833
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2396 2486 2513 2975 3012 3914
Size: 648 975 255 90 27 462 37 902
Cuts at: 3914 4273 4432 518
Size: 359 159 2042
Fragments arranged by size:
2042 975 902 648 462 359 255 159
90 37 27
MspA1I CmG'CkG
Cuts at: 1606 2343 2924 3372 4049 5197 5442 1606
Size: 737 581 448 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 581 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2703 513
Size: 552 957 681 3766
Fragments arranged by size:
3766 957 681 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3011 3314 4859 1132
Size: 97 1782 303 1545 2229
Fragments arranged by size:
2229 1782 1545 303 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PmlI CAC'GTG
Cuts at: 2394 2394
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 2619 2774 3094 3308 830
Size: 1789 155 320 214 3478
Fragments arranged by size:
3478 1789 320 214 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SapI GCTCTTCn'nnn_
Cuts at: 2030 2721 3129 2030
Size: 691 408 4857
Fragments arranged by size:
4857 691 408
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2224 2615 2770 3090 3304 4439 5120
Size: 219 1179 391 155 320 214 1135 681
Cuts at: 5120 5311 826
Size: 191 1471
Fragments arranged by size:
1471 1179 1135 681 391 320 219 214
191 155
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3253 3747 493
Size: 2760 494 2702
Fragments arranged by size:
2760 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3011 3314 1132
Size: 1879 303 3774
Fragments arranged by size:
3774 1879 303
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2022 2361 2703 3395 513
Size: 552 957 339 342 692 3074
Fragments arranged by size:
3074 957 692 552 342 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2394 2459 2612 2705 3983 4000 4153
Size: 1255 814 65 153 93 1278 17 153
Cuts at: 4153 4338 4783 325
Size: 185 445 1498
Fragments arranged by size:
1498 1278 1255 814 445 185 153 153
93 65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2587 2619 2978 3010 5916 5948 2587
Size: 32 359 32 2906 32 2595
Fragments arranged by size:
2906 2595 359 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 2671 3292 2671
Size: 621 5335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2256 2256
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI