Restriction map for
pFN21ASDA0617
(Circular) (Six-base) MAPSORT of: pFN21ASDA0617.seq Check: 6209 from: 1 to: 7033
pFN21ASDA0617 7033 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2439 2556 3658 4107 4311 4376 1937
Size: 502 117 1102 449 204 65 4594
Fragments arranged by size:
4594 1102 502 449 204 117 65
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 3310 5160 5533 1563
Size: 1747 1850 373 3063
Fragments arranged by size:
3063 1850 1747 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 3033 3086 3762 5096 6480 6748 1632
Size: 1065 336 53 676 1334 1384 268 1917
Fragments arranged by size:
1917 1384 1334 1065 676 336 268 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 3583 1166
Size: 2417 4616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AlwNI CAG_nnn'CTG
Cuts at: 4055 4776 6348 4055
Size: 721 1572 4740
Fragments arranged by size:
4740 1572 721
ApaLI G'TGCA_C
Cuts at: 3759 5090 6246 3759
Size: 1331 1156 4546
Fragments arranged by size:
4546 1331 1156
ApoI r'AATT_y
Cuts at: 1493 2305 2666 2690 2863 3479 3867 4342 4568
Size: 812 361 24 173 616 388 475 226
Cuts at: 4568 4720 6829 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 812 616 475 388 361 226
173 152 24
AseI AT'TA_AT
Cuts at: 160 1032 2453 2745 3827 5587 160
Size: 872 1421 292 1082 1760 1606
Fragments arranged by size:
1760 1606 1421 1082 872 292
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 4358 1870
Size: 73 2415 4545
Fragments arranged by size:
4545 2415 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3492 3525 3492
Size: 33 7000
BamHI G'GATC_C
Cuts at: 2073 4363 4730 2073
Size: 2290 367 4376
Fragments arranged by size:
4376 2290 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4354 5809 618
Size: 325 217 627 2567 1455 1842
Fragments arranged by size:
2567 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2193 3913 4352 1391
Size: 802 1720 439 4072
Fragments arranged by size:
4072 1720 802 439
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2849 2910 928
Size: 971 950 61 5051
Fragments arranged by size:
5051 971 950 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 2353 4009 1548
Size: 805 1656 4572
Fragments arranged by size:
4572 1656 805
BfrBI ATG'CAT
Cuts at: 2204 2204
Size: 7033
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3694 7028 3694
Size: 3334 3699
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 3763 5094 6250 1790
Size: 1973 1331 1156 2573
Fragments arranged by size:
2573 1973 1331 1156
BmgBI CAC'GTC
Cuts at: 1318 3423 1318
Size: 2105 4928
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4764 5722 442
Size: 416 53 83 399 24 3347 958 1753
Fragments arranged by size:
3347 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3151 3656 5693 1239
Size: 1912 505 2037 2579
Fragments arranged by size:
2579 2037 1912 505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5158 6023 6321 5158
Size: 865 298 5870
Fragments arranged by size:
5870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2609 3177 5696 882
Size: 617 1110 568 2519 2219
Fragments arranged by size:
2519 2219 1110 617 568
BsaAI yAC'GTr
Cuts at: 493 1204 4330 4824 493
Size: 711 3126 494 2702
Fragments arranged by size:
3126 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2072 7027 2072
Size: 4955 2078
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4840 5222 5857 275
Size: 53 83 186 936 3307 382 635 1451
Fragments arranged by size:
3307 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5466 5864 6138 6285 6696 5466
Size: 398 274 147 411 5803
Fragments arranged by size:
5803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 3149 3179 1178
Size: 30 165 30 1135 30 581 30 5032
Fragments arranged by size:
5032 1135 581 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 1186
Size: 1015 6018
BseYI C'CCAG_C
Cuts at: 6236 6732 6236
Size: 496 6537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 4483 5244 6272 1540
Size: 275 186 1081 1401 761 1028 2301
Fragments arranged by size:
2301 1401 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 3546 3763 5094 5179 6250 2193
Size: 1353 217 1331 85 1071 2976
Fragments arranged by size:
2976 1353 1331 1071 217 85
BsmI GAATG_Cn'
Cuts at: 4544 4637 4544
Size: 93 6940
BsmBI CGTCTCn'nnnn_
Cuts at: 3119 3119
Size: 7033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 3546 3763 3913 4352 5094 5179
Size: 399 403 1353 217 150 439 742 85
Cuts at: 5179 6250 1391
Size: 1071 2174
Fragments arranged by size:
2174 1353 1071 742 439 403 399 217
150 85
BspEI T'CCGG_A
Cuts at: 6696 6696
Size: 7033
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4388 844
Size: 3544 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2539 4920 1394
Size: 429 716 2381 3507
Fragments arranged by size:
3507 2381 716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3273 5528 5702 65
Size: 1183 2025 2255 174 1396
Fragments arranged by size:
2255 2025 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5677 5864 1700
Size: 110 3867 187 2869
Fragments arranged by size:
3867 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2394 2413 2919 96
Size: 2298 19 506 4210
Fragments arranged by size:
4210 2298 506 19
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 5093 6105 5093
Size: 1012 6021
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3796 794
Size: 3002 4031
BstBI TT'CG_AA
Cuts at: 2007 5837 2007
Size: 3830 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 2073 3061 3694 3737 4363 4730 5115 5132
Size: 101 988 633 43 626 367 385 17
Cuts at: 5132 6584 6670 6682 7028 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 988 633 626 385 367 346
101 86 43 17 12
BstZ17I GTA'TAC
Cuts at: 4312 4312
Size: 7033
Bsu36I CC'TnA_GG
Cuts at: 4296 4296
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 2094 513
Size: 552 1029 5452
Fragments arranged by size:
5452 1029 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 2770 4549 5343 5363 2770
Size: 1779 794 20 4440
Fragments arranged by size:
4440 1779 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3634 3669 529
Size: 35 3070 35 3893
Fragments arranged by size:
3893 3070 35 35
DraI TTT'AAA
Cuts at: 3121 4337 3121
Size: 1216 5817
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4480 5369 6998
Size: 54 1425 50 275 18 2650 889 1629
Cuts at: 6998 8
Size: 43
Fragments arranged by size:
2650 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4480 1537
Size: 275 2668 4090
Fragments arranged by size:
4090 2668 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3062 4963 1353
Size: 1709 1901 3423
Fragments arranged by size:
3423 1901 1709
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 2191
Size: 7033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 3033 3086 3151 3656 3762 5096
Size: 393 1065 336 53 65 505 106 1334
Cuts at: 5096 5693 6480 6748 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1334 1065 787 597 505 393 336
268 106 65 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2983 2983
Size: 7033
EcoO109I rG'GnC_Cy
Cuts at: 2125 2125
Size: 7033
EcoRI G'AATT_C
Cuts at: 2863 3479 4342 4720 2863
Size: 616 863 378 5176
Fragments arranged by size:
5176 863 616 378
EcoRV GAT'ATC
Cuts at: 3387 3886 3387
Size: 499 6534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2595 3630 2595
Size: 1035 5998
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 6180 1678
Size: 4502 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2587 2619 5674 5706 5748
Size: 32 135 32 1015 32 3055 32 42
Cuts at: 5748 5780 1373
Size: 32 2626
Fragments arranged by size:
3055 2626 1015 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3040 3659 4377 4623 669
Size: 988 281 1102 619 718 246 3079
Fragments arranged by size:
3079 1102 988 718 619 281 246
HindIII A'AGCT_T
Cuts at: 748 2343 748
Size: 1595 5438
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 2799 4632 2799
Size: 1833 5200
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3733 4098 6147 6331 1305
Size: 2428 365 2049 184 2007
Fragments arranged by size:
2428 2049 2007 365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5211
Fragments arranged by size:
5211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2226 2390 3470 3583 3827 4991
Size: 648 900 160 164 1080 113 244 1164
Cuts at: 4991 5350 5509 518
Size: 359 159 2042
Fragments arranged by size:
2042 1164 1080 900 648 359 244 164
160 159 113
MspA1I CmG'CkG
Cuts at: 1606 3946 4449 5126 6274 6519 1606
Size: 2340 503 677 1148 245 2120
Fragments arranged by size:
2340 2120 1148 677 503 245
NcoI C'CATG_G
Cuts at: 513 1065 2094 513
Size: 552 1029 5452
Fragments arranged by size:
5452 1029 552
NdeI CA'TA_TG
Cuts at: 387 2880 3681 4318 387
Size: 2493 801 637 3102
Fragments arranged by size:
3102 2493 801 637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NsiI A_TGCA'T
Cuts at: 2206 2206
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 2920 3614 4391 5936 1132
Size: 97 1691 694 777 1545 2229
Fragments arranged by size:
2229 1691 1545 777 694 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7033
PfoI T'CCnGG_A
Cuts at: 3090 3090
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2125
Size: 7033
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 3318 4603 3318
Size: 1285 5748
PstI C_TGCA'G
Cuts at: 830 2665 3694 4385 830
Size: 1835 1029 691 3478
Fragments arranged by size:
3478 1835 1029 691
PvuI CG_AT'CG
Cuts at: 2001 3082 2001
Size: 1081 5952
SacI G_AGCT'C
Cuts at: 2193 2193
Size: 7033
SalI G'TCGA_C
Cuts at: 1936 3657 4375 1936
Size: 1721 718 4594
Fragments arranged by size:
4594 1721 718
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 5281 6993 5281
Size: 1712 5321
SfcI C'TryA_G
Cuts at: 826 1045 2194 2661 2847 3494 3690 3914 4140
Size: 219 1149 467 186 647 196 224 226
Cuts at: 4140 4381 5516 6197 6388 826
Size: 241 1135 681 191 1471
Fragments arranged by size:
1471 1149 1135 681 647 467 241 226
224 219 196 191 186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5137 6038 6300 820
Size: 197 926 3194 901 262 1553
Fragments arranged by size:
3194 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4330 4824 493
Size: 3837 494 2702
Fragments arranged by size:
3837 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 3255 4280 4957 5
Size: 47 3203 1025 677 2081
Fragments arranged by size:
3203 2081 1025 677 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2094 2908 4472 513
Size: 552 1029 814 1564 3074
Fragments arranged by size:
3074 1564 1029 814 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2475 5060 5077 5230 5415 5860 325
Size: 1255 895 2585 17 153 185 445 1498
Fragments arranged by size:
2585 1498 1255 895 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2299 2394 2413
Size: 274 80 33 51 1443 322 95 19
Cuts at: 2413 2875 2919 5279 6991 96
Size: 462 44 2360 1712 138
Fragments arranged by size:
2360 1712 1443 462 322 274 138 95
80 51 44 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6993 7025 6993
Size: 32 7001
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 4160 4369 4160
Size: 209 6824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 2670 5162 2670
Size: 2492 4541
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BstXI DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI