Restriction map for
pFN21ASDA0615
(Circular) (Six-base) MAPSORT of: pFN21ASDA0615.seq Check: 4290 from: 1 to: 7417
pFN21ASDA0615 7417 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5227 278
Size: 53 83 186 936 3691 2468
Fragments arranged by size:
3691 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4760 1937
Size: 2823 4594
Acc65I G'GTAC_C
Cuts at: 4738 4738
Size: 7417
AclI AA'CG_TT
Cuts at: 1563 3655 5544 5917 1563
Size: 2092 1889 373 3063
Fragments arranged by size:
3063 2092 1889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 4319 4396 4628 5480 6864 7132 1632
Size: 1281 1406 77 232 852 1384 268 1917
Fragments arranged by size:
1917 1406 1384 1281 852 268 232 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7220
AflIII A'CryG_T
Cuts at: 1203 4260 6316 7293 1203
Size: 3057 2056 977 1327
Fragments arranged by size:
3057 2056 1327 977
AgeI A'CCGG_T
Cuts at: 6248 6248
Size: 7417
AhdI GACnn_n'nnGTC
Cuts at: 6146 6146
Size: 7417
AleI CACnn'nnGTG
Cuts at: 1166 4265 1166
Size: 3099 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 1184
Size: 1011 6406
AlwNI CAG_nnn'CTG
Cuts at: 3482 3794 4549 5160 6732 3482
Size: 312 755 611 1572 4167
Fragments arranged by size:
4167 1572 755 611 312
ApaLI G'TGCA_C
Cuts at: 5474 6630 5474
Size: 1156 6261
ApoI r'AATT_y
Cuts at: 1493 2498 2862 3019 3209 3344 4202 4726 4952
Size: 1005 364 157 190 135 858 524 226
Cuts at: 4952 5104 7213 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1005 858 524 364 226 190
157 152 135
AseI AT'TA_AT
Cuts at: 160 1032 3402 5971 160
Size: 872 2370 2569 1606
Fragments arranged by size:
2569 2370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 4742 1870
Size: 73 88 2711 4545
Fragments arranged by size:
4545 2711 88 73
BamHI G'GATC_C
Cuts at: 4747 5114 4747
Size: 367 7050
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4424 4738 6193 618
Size: 325 217 627 2637 314 1455 1842
Fragments arranged by size:
2637 1842 1455 627 325 314 217
BanII G_rGCy'C
Cuts at: 1391 4074 4477 4736 1391
Size: 2683 403 259 4072
Fragments arranged by size:
4072 2683 403 259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3207 928
Size: 971 1308 5138
Fragments arranged by size:
5138 1308 971
BbvCI CC'TCA_GC
Cuts at: 2151 2394 4497 4592 2151
Size: 243 2103 95 4976
Fragments arranged by size:
4976 2103 243 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2036 2070 5608 5642 1903
Size: 34 99 34 3538 34 3678
Fragments arranged by size:
3678 3538 99 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2329 3240 5309 6519 2329
Size: 911 2069 1210 3227
Fragments arranged by size:
3227 2069 1210 911
BclI T'GATC_A
Cuts at: 1548 3772 1548
Size: 2224 5193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2115 2376 6028
Size: 107 122 71 631 605 443 261 3652
Cuts at: 6028 136
Size: 1525
Fragments arranged by size:
3652 1525 631 605 443 261 122 107
71
BglII A'GATC_T
Cuts at: 4568 4611 4650 4678 7412 4568
Size: 43 39 28 2734 4573
Fragments arranged by size:
4573 2734 43 39 28
BlpI GC'TnA_GC
Cuts at: 4834 4834
Size: 7417
Bme1580I G_kGCm'C
Cuts at: 1790 4381 5478 6634 1790
Size: 2591 1097 1156 2573
Fragments arranged by size:
2591 2573 1156 1097
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2460 3804 4527
Size: 416 53 83 399 24 1043 1344 723
Cuts at: 4527 5148 6106 442
Size: 621 958 1753
Fragments arranged by size:
1753 1344 1043 958 723 621 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2667 4237 2667
Size: 1570 5847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 4049 4523 6077 1239
Size: 863 1947 474 1554 2579
Fragments arranged by size:
2579 1947 1554 863 474
Bpu10I CC'TnA_GC
Cuts at: 2151 2394 4497 4592 2151
Size: 243 2103 95 4976
Fragments arranged by size:
4976 2103 243 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5542 6407 6705 5542
Size: 865 298 6254
Fragments arranged by size:
6254 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3230 6080 882
Size: 617 1731 2850 2219
Fragments arranged by size:
2850 2219 1731 617
BsaAI yAC'GTr
Cuts at: 493 1204 4714 5208 493
Size: 711 3510 494 2702
Fragments arranged by size:
3510 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7411 7411
Size: 7417
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5224 5606 6241 275
Size: 53 83 186 936 3691 382 635 1451
Fragments arranged by size:
3691 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5850 6248 6522 6669 7080 5850
Size: 398 274 147 411 6187
Fragments arranged by size:
6187 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3691 3721 1178
Size: 30 165 30 2288 30 4874
Fragments arranged by size:
4874 2288 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2615 3666 4340 1186
Size: 982 447 1051 674 4263
Fragments arranged by size:
4263 1051 982 674 447
BseYI C'CCAG_C
Cuts at: 2741 3726 4059 4406 6620 7116 2741
Size: 985 333 347 2214 496 3042
Fragments arranged by size:
3042 2214 985 496 347 333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6844
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2098 4867 5628 6656 1540
Size: 275 186 97 2769 761 1028 2301
Fragments arranged by size:
2769 2301 1028 761 275 186 97
BsiHKAI G_wGCw'C
Cuts at: 2136 2211 4074 5478 5563 6634 2136
Size: 75 1863 1404 85 1071 2919
Fragments arranged by size:
2919 1863 1404 1071 85 75
BsmI GAATG_Cn'
Cuts at: 2355 2680 4928 5021 2355
Size: 325 2248 93 4751
Fragments arranged by size:
4751 2248 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2211 4074 4381 4477 4736 5478
Size: 399 346 75 1863 307 96 259 742
Cuts at: 5478 5563 6634 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1863 1071 742 399 346 307 259
96 85 75
BspEI T'CCGG_A
Cuts at: 7080 7080
Size: 7417
BspHI T'CATG_A
Cuts at: 4129 5306 7036 4129
Size: 1177 1730 4510
Fragments arranged by size:
4510 1730 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4557 4772 844
Size: 3713 215 3489
Fragments arranged by size:
3713 3489 215
BsrBI CCG'CTC
Cuts at: 1394 1823 5304 1394
Size: 429 3481 3507
Fragments arranged by size:
3507 3481 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3904 5912 6086 65
Size: 1183 2656 2008 174 1396
Fragments arranged by size:
2656 2008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6061 6248 1700
Size: 110 4251 187 2869
Fragments arranged by size:
4251 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7417
BssHII G'CGCG_C
Cuts at: 1925 2139 2168 1925
Size: 214 29 7174
Fragments arranged by size:
7174 214 29
BssSI C'ACGA_G
Cuts at: 5477 6489 5477
Size: 1012 6405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4549 794
Size: 3755 3662
BstBI TT'CG_AA
Cuts at: 2007 6221 2007
Size: 4214 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7417
BstXI CCAn_nnnn'nTGG
Cuts at: 4705 4705
Size: 7417
BstYI r'GATC_y
Cuts at: 1972 4568 4611 4650 4678 4747 5114 5499 5516
Size: 2596 43 39 28 69 367 385 17
Cuts at: 5516 6968 7054 7066 7412 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2596 1977 1452 385 367 346 86 69
43 39 28 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2092 3090 3913 4698 513
Size: 552 957 70 998 823 785 3232
Fragments arranged by size:
3232 998 957 823 785 552 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6678
Fragments arranged by size:
6678 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2284 3719 4514 4933 5727 5747 2052
Size: 232 1435 795 419 794 20 3722
Fragments arranged by size:
3722 1435 795 794 419 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4150 4185 529
Size: 35 3586 35 3761
Fragments arranged by size:
3761 3586 35 35
DraI TTT'AAA
Cuts at: 3085 4721 3085
Size: 1636 5781
DraIII CAC_nnn'GTG
Cuts at: 2597 2597
Size: 7417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6424 809
Size: 507 5108 1802
Fragments arranged by size:
5108 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 4864 5753
Size: 54 1425 50 275 18 265 2769 889
Cuts at: 5753 7382 8
Size: 1629 43
Fragments arranged by size:
2769 1629 1425 889 275 265 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2095 4864 1537
Size: 275 283 2769 4090
Fragments arranged by size:
4090 2769 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2718 2835 5347 1353
Size: 1365 117 2512 3423
Fragments arranged by size:
3423 2512 1365 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2219 6004 6378 6524 387
Size: 1064 138 498 132 3785 374 146 1280
Fragments arranged by size:
3785 1280 1064 498 374 146 138 132
EcoICRI GAG'CTC
Cuts at: 4072 4072
Size: 7417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2913 4049 4319 4396 4523 4628
Size: 393 470 811 1136 270 77 127 105
Cuts at: 4628 5480 6077 6864 7132 1239
Size: 852 597 787 268 1524
Fragments arranged by size:
1524 1136 852 811 787 597 470 393
270 268 127 105 77
EcoO109I rG'GnC_Cy
Cuts at: 2107 3764 2107
Size: 1657 5760
EcoRI G'AATT_C
Cuts at: 2498 4726 5104 2498
Size: 2228 378 4811
Fragments arranged by size:
4811 2228 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2864 3570 4140 4367 2864
Size: 706 570 227 5914
Fragments arranged by size:
5914 706 570 227
FspI TGC'GCA
Cuts at: 5923 5923
Size: 7417
HaeII r_GCGC'y
Cuts at: 1678 2217 6564 1678
Size: 539 4347 2531
Fragments arranged by size:
4347 2531 539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2851 2883 3740 3772 6058
Size: 32 135 32 1279 32 857 32 2286
Cuts at: 6058 6090 6132 6164 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2286 1279 857 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4761 5007 669
Size: 988 281 2823 246 3079
Fragments arranged by size:
3079 2823 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2323 3974 748
Size: 1575 1651 4191
Fragments arranged by size:
4191 1651 1575
HpaI GTT'AAC
Cuts at: 5007 5007
Size: 7417
KpnI G_GTAC'C
Cuts at: 4742 4742
Size: 7417
MfeI C'AATT_G
Cuts at: 3951 5016 3951
Size: 1065 6352
MluI A'CGCG_T
Cuts at: 7293 7293
Size: 7417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4481 4686 6531 6715 1305
Size: 3176 205 1845 184 2007
Fragments arranged by size:
3176 2007 1845 205 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5595
Fragments arranged by size:
5595 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3128 4178 4265 4280 5375 5734 5893
Size: 648 1962 1050 87 15 1095 359 159
Cuts at: 5893 518
Size: 2042
Fragments arranged by size:
2042 1962 1095 1050 648 359 159 87
15
MspA1I CmG'CkG
Cuts at: 1606 2063 2094 2161 3218 3730 4833 5510 6658
Size: 457 31 67 1057 512 1103 677 1148
Cuts at: 6658 6903 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1103 1057 677 512 457 245
67 31
NcoI C'CATG_G
Cuts at: 513 1065 2022 3090 3913 4698 513
Size: 552 957 1068 823 785 3232
Fragments arranged by size:
3232 1068 957 823 785 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7417
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7417
NotI GC'GGCC_GC
Cuts at: 4864 4864
Size: 7417
NspI r_CATG'y
Cuts at: 1132 1229 2284 4399 4775 6320 1132
Size: 97 1055 2115 376 1545 2229
Fragments arranged by size:
2229 2115 1545 1055 376 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7417
PciI A'CATG_T
Cuts at: 6316 6316
Size: 7417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2822 4654 1108
Size: 1714 1832 3871
Fragments arranged by size:
3871 1832 1714
PfoI T'CCnGG_A
Cuts at: 4065 4065
Size: 7417
PmeI GTTT'AAAC
Cuts at: 4721 4721
Size: 7417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5486 5518 6999 7031 5486
Size: 32 1481 32 5872
Fragments arranged by size:
5872 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3764 3764
Size: 7417
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7417
PsiI TTA'TAA
Cuts at: 4987 4987
Size: 7417
PstI C_TGCA'G
Cuts at: 830 2184 4257 4579 4769 830
Size: 1354 2073 322 190 3478
Fragments arranged by size:
3478 2073 1354 322 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7417
PvuII CAG'CTG
Cuts at: 2063 3218 3730 2063
Size: 1155 512 5750
Fragments arranged by size:
5750 1155 512
SacI G_AGCT'C
Cuts at: 4074 4074
Size: 7417
SacII CC_GC'GG
Cuts at: 2095 2095
Size: 7417
SalI G'TCGA_C
Cuts at: 1936 4759 1936
Size: 2823 4594
SbfI CC_TGCA'GG
Cuts at: 4769 4769
Size: 7417
ScaI AGT'ACT
Cuts at: 5665 7377 5665
Size: 1712 5705
SfcI C'TryA_G
Cuts at: 826 1045 2180 4253 4575 4765 5900 6581 6772
Size: 219 1135 2073 322 190 1135 681 191
Cuts at: 6772 826
Size: 1471
Fragments arranged by size:
2073 1471 1135 1135 681 322 219 191
190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7417
SmaI CCC'GGG
Cuts at: 1872 2033 4744 1872
Size: 161 2711 4545
Fragments arranged by size:
4545 2711 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5521 6422 6684 820
Size: 197 926 3578 901 262 1553
Fragments arranged by size:
3578 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4714 5208 493
Size: 4221 494 2702
Fragments arranged by size:
4221 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7417
SphI G_CATG'C
Cuts at: 1132 4775 1132
Size: 3643 3774
SrfI GCCC'GGGC
Cuts at: 2033 2033
Size: 7417
SspI AAT'ATT
Cuts at: 5 52 2695 3965 4080 5341 5
Size: 47 2643 1270 115 1261 2081
Fragments arranged by size:
2643 2081 1270 1261 115 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7417
StyI C'CwwG_G
Cuts at: 513 1065 2022 2217 2274 3090 3913 4698 4856
Size: 552 957 195 57 816 823 785 158
Cuts at: 4856 513
Size: 3074
Fragments arranged by size:
3074 957 823 816 785 552 195 158
57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4442 4680 5444 5461 5614 5799 6244
Size: 1255 2862 238 764 17 153 185 445
Cuts at: 6244 325
Size: 1498
Fragments arranged by size:
2862 1498 1255 764 445 238 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4257 5663 7375
Size: 274 80 33 51 1443 2280 1406 1712
Cuts at: 7375 96
Size: 138
Fragments arranged by size:
2280 1712 1443 1406 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7377 7409 7377
Size: 32 7385
Tth111I GACn'n_nGTC
Cuts at: 1316 2780 1316
Size: 1464 5953
XbaI T'CTAG_A
Cuts at: 2699 4753 2699
Size: 2054 5363
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7417
XmaI C'CCGG_G
Cuts at: 1870 2031 4742 1870
Size: 161 2711 4545
Fragments arranged by size:
4545 2711 161
XmnI GAAnn'nnTTC
Cuts at: 2931 3926 4123 4602 4613 5546 2931
Size: 995 197 479 11 933 4802
Fragments arranged by size:
4802 995 933 479 197 11
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5225 276
Size: 53 83 186 936 3691 2468
Fragments arranged by size:
3691 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SwaI XcmI