Restriction map for
pFN21ASDA0613
(Circular) (Six-base) MAPSORT of: pFN21ASDA0613.seq Check: 8572 from: 1 to: 6910
pFN21ASDA0613 6910 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3001 3163 3424 3926 4109 3001
Size: 162 261 502 183 5802
Fragments arranged by size:
5802 502 261 183 162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4720 278
Size: 53 83 186 936 3184 2468
Fragments arranged by size:
3184 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2739 2757 3243 4253 1937
Size: 802 18 486 1010 4594
Fragments arranged by size:
4594 1010 802 486 18
Acc65I G'GTAC_C
Cuts at: 4231 4231
Size: 6910
AclI AA'CG_TT
Cuts at: 1563 3807 5037 5410 1563
Size: 2244 1230 373 3063
Fragments arranged by size:
3063 2244 1230 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3132 3759 4973 6357 6625 1632
Size: 1500 627 1214 1384 268 1917
Fragments arranged by size:
1917 1500 1384 1214 627 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6713
AflIII A'CryG_T
Cuts at: 1203 5809 6786 1203
Size: 4606 977 1327
Fragments arranged by size:
4606 1327 977
AgeI A'CCGG_T
Cuts at: 5741 5741
Size: 6910
AhdI GACnn_n'nnGTC
Cuts at: 5639 5639
Size: 6910
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3799 3855 1184
Size: 2615 56 4239
Fragments arranged by size:
4239 2615 56
AlwNI CAG_nnn'CTG
Cuts at: 3041 3080 3155 3257 3562 4653 6225 3041
Size: 39 75 102 305 1091 1572 3726
Fragments arranged by size:
3726 1572 1091 305 102 75 39
ApaI G_GGCC'C
Cuts at: 2052 2058 2554 3185 3464 3704 2052
Size: 6 496 631 279 240 5258
Fragments arranged by size:
5258 631 496 279 240 6
ApaLI G'TGCA_C
Cuts at: 2944 4967 6123 2944
Size: 2023 1156 3731
Fragments arranged by size:
3731 2023 1156
ApoI r'AATT_y
Cuts at: 1493 2876 4219 4445 4597 6706 1493
Size: 1383 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1383 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5464 160
Size: 872 4432 1606
Fragments arranged by size:
4432 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2553 2587 2689 3207 3526 3567
Size: 73 108 502 34 102 518 319 41
Cuts at: 3567 4235 1870
Size: 668 4545
Fragments arranged by size:
4545 668 518 502 319 108 102 73
41 34
BamHI G'GATC_C
Cuts at: 4240 4607 4240
Size: 367 6543
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 2414 2423 3604 3732
Size: 325 217 627 590 37 9 1181 128
Cuts at: 3732 4231 5686 618
Size: 499 1455 1842
Fragments arranged by size:
1842 1455 1181 627 590 499 325 217
128 37 9
BanII G_rGCy'C
Cuts at: 1391 2052 2058 2436 2554 2588 3185 3464 3704
Size: 661 6 378 118 34 597 279 240
Cuts at: 3704 3987 4229 1391
Size: 283 242 4072
Fragments arranged by size:
4072 661 597 378 283 279 242 240
118 34 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3981 928
Size: 971 2082 3857
Fragments arranged by size:
3857 2082 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5101 5135 1903
Size: 34 3164 34 3678
Fragments arranged by size:
3678 3164 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4802 6012 4802
Size: 1210 5700
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6910
BfrBI ATG'CAT
Cuts at: 3889 3889
Size: 6910
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5521 136
Size: 107 122 71 631 605 3849 1525
Fragments arranged by size:
3849 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6905 6905
Size: 6910
BlpI GC'TnA_GC
Cuts at: 2150 3639 4327 2150
Size: 1489 688 4733
Fragments arranged by size:
4733 1489 688
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2058 2554 2815 2948 3185 3464 3704
Size: 262 6 496 261 133 237 279 240
Cuts at: 3704 4971 6127 1790
Size: 1267 1156 2573
Fragments arranged by size:
2573 1267 1156 496 279 262 261 240
237 133 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6910
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 2134 3003
Size: 416 53 83 399 24 638 79 869
Cuts at: 3003 4641 5599 442
Size: 1638 958 1753
Fragments arranged by size:
1753 1638 958 869 638 416 399 83
79 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6910
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2732
Size: 6910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2289 4154 5570 1239
Size: 1050 1865 1416 2579
Fragments arranged by size:
2579 1865 1416 1050
Bpu10I CC'TnA_GC
Cuts at: 2435 2435
Size: 6910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3916 5035 5900 6198 2269
Size: 1647 1119 865 298 2981
Fragments arranged by size:
2981 1647 1119 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 5573 882
Size: 617 1131 2943 2219
Fragments arranged by size:
2943 2219 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 4207 4701 493
Size: 711 3003 494 2702
Fragments arranged by size:
3003 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6904 6904
Size: 6910
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 4717 5099 5734
Size: 53 83 186 936 651 2533 382 635
Cuts at: 5734 275
Size: 1451
Fragments arranged by size:
2533 1451 936 651 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5343 5741 6015 6162 6573 5343
Size: 398 274 147 411 5680
Fragments arranged by size:
5680 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2287 2317 3299 3323 3329
Size: 30 165 30 884 30 982 24 6
Cuts at: 3329 3353 3367 3397 1178
Size: 24 14 30 4691
Fragments arranged by size:
4691 982 884 165 30 30 30 30
24 24 14 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2470 1186
Size: 1284 5626
BseYI C'CCAG_C
Cuts at: 2145 2381 2387 2985 3391 3577 3589 3649 6113
Size: 236 6 598 406 186 12 60 2464
Cuts at: 6113 6609 2145
Size: 496 2446
Fragments arranged by size:
2464 2446 598 496 406 236 186 60
12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3180 3420 1163
Size: 573 1444 240 4653
Fragments arranged by size:
4653 1444 573 240
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4360 5121 6149 1540
Size: 275 186 2359 761 1028 2301
Fragments arranged by size:
2359 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 2948 3539 4971 5056 6127 2436
Size: 512 591 1432 85 1071 3219
Fragments arranged by size:
3219 1432 1071 591 512 85
BsmI GAATG_Cn'
Cuts at: 4421 4514 4421
Size: 93 6817
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2058 2436 2554 2588 2815 2948
Size: 399 262 6 378 118 34 227 133
Cuts at: 2948 3185 3464 3539 3704 3987 4229 4971 5056
Size: 237 279 75 165 283 242 742 85
Cuts at: 5056 6127 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 283 279 262
242 237 227 165 133 118 85 75
34 6
BspEI T'CCGG_A
Cuts at: 6573 6573
Size: 6910
BspHI T'CATG_A
Cuts at: 4799 6529 4799
Size: 1730 5180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3001 3163 3424 3926 4109 4265 844
Size: 1994 163 162 261 502 183 156 3489
Fragments arranged by size:
3489 1994 502 261 183 163 162 156
BsrBI CCG'CTC
Cuts at: 1394 1823 2351 4797 1394
Size: 429 528 2446 3507
Fragments arranged by size:
3507 2446 528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5405 5579 65
Size: 1183 4157 174 1396
Fragments arranged by size:
4157 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2323 2938 5554 5741 1700
Size: 110 513 615 2616 187 2869
Fragments arranged by size:
2869 2616 615 513 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6910
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6910
BssSI C'ACGA_G
Cuts at: 2165 4970 5982 2165
Size: 2805 1012 3093
Fragments arranged by size:
3093 2805 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3562 4026 794
Size: 2768 464 3678
Fragments arranged by size:
3678 2768 464
BstBI TT'CG_AA
Cuts at: 2007 5714 2007
Size: 3707 3203
BstEII G'GTnAC_C
Cuts at: 1184 2159 1184
Size: 975 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 3874 3874
Size: 6910
BstYI r'GATC_y
Cuts at: 1972 4240 4607 4992 5009 6461 6547 6559 6905
Size: 2268 367 385 17 1452 86 12 346
Cuts at: 6905 1972
Size: 1977
Fragments arranged by size:
2268 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2394 2525 2642 3600 2394
Size: 131 117 958 5704
Fragments arranged by size:
5704 958 131 117
BtgI C'CryG_G
Cuts at: 513 1065 2513 3717 4030 4047 513
Size: 552 1448 1204 313 17 3376
Fragments arranged by size:
3376 1448 1204 552 313 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3040 3835 487
Size: 683 56 1814 795 3562
Fragments arranged by size:
3562 1814 795 683 56
BtsI GCAGTG_nn'
Cuts at: 3048 3141 3219 3679 4426 5220 5240 3048
Size: 93 78 460 747 794 20 4718
Fragments arranged by size:
4718 794 747 460 93 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6910
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6875
DraI TTT'AAA
Cuts at: 4214 4214
Size: 6910
DraIII CAC_nnn'GTG
Cuts at: 4026 4026
Size: 6910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5917 809
Size: 507 4601 1802
Fragments arranged by size:
4601 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 3030 3138
Size: 54 1425 50 275 18 342 858 108
Cuts at: 3138 4357 5246 6875 8
Size: 1219 889 1629 43
Fragments arranged by size:
1629 1425 1219 889 858 342 275 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4357 1537
Size: 275 2545 4090
Fragments arranged by size:
4090 2545 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3124 3151 3735 3789 3968 4840 1353
Size: 1771 27 584 54 179 872 3423
Fragments arranged by size:
3423 1771 872 584 179 54 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2461 3052 5497 5871 6017 387
Size: 1064 138 872 591 2445 374 146 1280
Fragments arranged by size:
2445 1280 1064 872 591 374 146 138
EcoICRI GAG'CTC
Cuts at: 2434 2434
Size: 6910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2289 3132 3759 4154 4973 5570 6357
Size: 393 657 843 627 395 819 597 787
Cuts at: 6357 6625 1239
Size: 268 1524
Fragments arranged by size:
1524 843 819 787 657 627 597 395
393 268
EcoO109I rG'GnC_Cy
Cuts at: 2055 2344 2550 2557 2728 3108 3181 3182 3460
Size: 289 206 7 171 380 73 1 278
Cuts at: 3460 3461 3596 3700 2055
Size: 1 135 104 5265
Fragments arranged by size:
5265 380 289 278 206 171 135 104
73 7 1 1
EcoRI G'AATT_C
Cuts at: 2876 4219 4597 2876
Size: 1343 378 5189
Fragments arranged by size:
5189 1343 378
FspI TGC'GCA
Cuts at: 4114 5416 4114
Size: 1302 5608
HaeII r_GCGC'y
Cuts at: 1678 2748 6057 1678
Size: 1070 3309 2531
Fragments arranged by size:
3309 2531 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2287 2319 2732 2764 3494
Size: 32 135 32 715 32 413 32 730
Cuts at: 3494 3526 5551 5583 5625 5657 1373
Size: 32 2025 32 42 32 2626
Fragments arranged by size:
2626 2025 730 715 413 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 4254 4500 669
Size: 988 281 250 2066 246 3079
Fragments arranged by size:
3079 2066 988 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6910
HpaI GTT'AAC
Cuts at: 4500 4500
Size: 6910
KpnI G_GTAC'C
Cuts at: 4235 4235
Size: 6910
MfeI C'AATT_G
Cuts at: 4509 4509
Size: 6910
MluI A'CGCG_T
Cuts at: 6786 6786
Size: 6910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2677 2850 6024 6208 1305
Size: 1372 173 3174 184 2007
Fragments arranged by size:
3174 2007 1372 184 173
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 3032 10
Size: 54 1425 343 342 858 3888
Fragments arranged by size:
3888 1425 858 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4868 5227 5386 518
Size: 648 3702 359 159 2042
Fragments arranged by size:
3702 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2515 2968 3038 3456 3593 3847 4326 5003
Size: 909 453 70 418 137 254 479 677
Cuts at: 5003 6151 6396 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 909 677 479 453 418 254
245 137 70
NcoI C'CATG_G
Cuts at: 513 1065 3717 4030 513
Size: 552 2652 313 3393
Fragments arranged by size:
3393 2652 552 313
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6910
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6910
NotI GC'GGCC_GC
Cuts at: 4357 4357
Size: 6910
NsiI A_TGCA'T
Cuts at: 3891 3891
Size: 6910
NspI r_CATG'y
Cuts at: 1132 1229 2098 2936 3893 4268 5813 1132
Size: 97 869 838 957 375 1545 2229
Fragments arranged by size:
2229 1545 957 869 838 375 97
PasI CC'CwG_GG
Cuts at: 1348 2058 3281 3489 4078 1348
Size: 710 1223 208 589 4180
Fragments arranged by size:
4180 1223 710 589 208
PciI A'CATG_T
Cuts at: 5809 5809
Size: 6910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6910
PfoI T'CCnGG_A
Cuts at: 2111 2111
Size: 6910
PmeI GTTT'AAAC
Cuts at: 4214 4214
Size: 6910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4979 5011 6492 6524 4979
Size: 32 1481 32 5365
Fragments arranged by size:
5365 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2344 2557 2728 3596 2344
Size: 213 171 868 5658
Fragments arranged by size:
5658 868 213 171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6910
PsiI TTA'TAA
Cuts at: 4480 4480
Size: 6910
PspOMI G'GGCC_C
Cuts at: 2048 2054 2550 3181 3460 3700 2048
Size: 6 496 631 279 240 5258
Fragments arranged by size:
5258 631 496 279 240 6
PspXI vC'TCGA_Gb
Cuts at: 2587 2587
Size: 6910
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 6878
PstI C_TGCA'G
Cuts at: 830 2076 2265 2835 4262 830
Size: 1246 189 570 1427 3478
Fragments arranged by size:
3478 1427 1246 570 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6910
PvuII CAG'CTG
Cuts at: 3038 3038
Size: 6910
SacI G_AGCT'C
Cuts at: 2436 2436
Size: 6910
SacII CC_GC'GG
Cuts at: 2516 2516
Size: 6910
SalI G'TCGA_C
Cuts at: 1936 4252 1936
Size: 2316 4594
SapI GCTCTTCn'nnn_
Cuts at: 3789 3789
Size: 6910
SbfI CC_TGCA'GG
Cuts at: 4262 4262
Size: 6910
ScaI AGT'ACT
Cuts at: 5158 6870 5158
Size: 1712 5198
SfcI C'TryA_G
Cuts at: 826 1045 2072 2261 2831 3124 3172 3268 3451
Size: 219 1027 189 570 293 48 96 183
Cuts at: 3451 4258 5393 6074 6265 826
Size: 807 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 807 681 570 293 219
191 189 183 96 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
SgrAI Cr'CCGG_yG
Cuts at: 1700 2938 1700
Size: 1238 5672
SmaI CCC'GGG
Cuts at: 1872 2053 2555 3209 3528 3569 4237 1872
Size: 181 502 654 319 41 668 4545
Fragments arranged by size:
4545 668 654 502 319 181 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2248 2587 3895 5014 5915 6177
Size: 197 926 305 339 1308 1119 901 262
Cuts at: 6177 820
Size: 1553
Fragments arranged by size:
1553 1308 1119 926 901 339 305 262
197
SnaBI TAC'GTA
Cuts at: 493 4207 4701 493
Size: 3714 494 2702
Fragments arranged by size:
3714 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6910
SphI G_CATG'C
Cuts at: 1132 2936 3893 4268 1132
Size: 1804 957 375 3774
Fragments arranged by size:
3774 1804 957 375
SrfI GCCC'GGGC
Cuts at: 2053 2053
Size: 6910
SspI AAT'ATT
Cuts at: 5 52 4834 5
Size: 47 4782 2081
Fragments arranged by size:
4782 2081 47
StuI AGG'CCT
Cuts at: 1517 2495 2687 3116 1517
Size: 978 192 429 5311
Fragments arranged by size:
5311 978 429 192
StyI C'CwwG_G
Cuts at: 513 1065 3104 3717 4030 4349 513
Size: 552 2039 613 313 319 3074
Fragments arranged by size:
3074 2039 613 552 319 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2395 3176 3214 3481 4937 4954 5107
Size: 1255 815 781 38 267 1456 17 153
Cuts at: 5107 5292 5737 325
Size: 185 445 1498
Fragments arranged by size:
1498 1456 1255 815 781 445 267 185
153 38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 5156 6868
Size: 274 80 33 51 1443 648 2531 1712
Cuts at: 6868 96
Size: 138
Fragments arranged by size:
2531 1712 1443 648 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 3367 3399 6870 6902 2292
Size: 32 1043 32 3471 32 2300
Fragments arranged by size:
3471 2300 1043 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2183 1316
Size: 867 6043
XbaI T'CTAG_A
Cuts at: 4246 4246
Size: 6910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2206
Size: 6910
XhoI C'TCGA_G
Cuts at: 1943 2587 1943
Size: 644 6266
XmaI C'CCGG_G
Cuts at: 1870 2051 2553 3207 3526 3567 4235 1870
Size: 181 502 654 319 41 668 4545
Fragments arranged by size:
4545 668 654 502 319 181 41
XmnI GAAnn'nnTTC
Cuts at: 5039 5039
Size: 6910
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4718 276
Size: 53 83 186 936 3184 2468
Fragments arranged by size:
3184 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI RsrII SanDI
SexAI SfiI SfoI SwaI