(Circular) (Six-base) MAPSORT of: pFN21ASDA0601.seq Check: 2313 from: 1 to: 7285 pFN21ASDA0601 7285 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5095 278 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4523 4628 1937 Size: 2586 105 4594 Fragments arranged by size: 4594 2586 105 Acc65I G'GTAC_C Cuts at: 4606 4606 Size: 7285 AclI AA'CG_TT Cuts at: 1563 5412 5785 1563 Size: 3849 373 3063 Fragments arranged by size: 3849 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2547 3441 3530 3798 3932 5348 6732 7000 Size: 915 894 89 268 134 1416 1384 268 Cuts at: 7000 1632 Size: 1917 Fragments arranged by size: 1917 1416 1384 915 894 268 268 134 89 AflII C'TTAA_G Cuts at: 820 1017 3668 820 Size: 197 2651 4437 Fragments arranged by size: 4437 2651 197 AflIII A'CryG_T Cuts at: 1203 6184 7161 1203 Size: 4981 977 1327 Fragments arranged by size: 4981 1327 977 AgeI A'CCGG_T Cuts at: 3171 6116 3171 Size: 2945 4340 AhdI GACnn_n'nnGTC Cuts at: 6014 6014 Size: 7285 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7285 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7285 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3114 3146 4416 4448 3114 Size: 32 1270 32 5951 Fragments arranged by size: 5951 1270 32 32 AlwNI CAG_nnn'CTG Cuts at: 5028 6600 5028 Size: 1572 5713 ApaI G_GGCC'C Cuts at: 2162 2162 Size: 7285 ApaLI G'TGCA_C Cuts at: 5342 6498 5342 Size: 1156 6129 ApoI r'AATT_y Cuts at: 1493 3370 3632 4594 4820 4972 7081 1493 Size: 1877 262 962 226 152 2109 1697 Fragments arranged by size: 2109 1877 1697 962 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 5839 160 Size: 872 4807 1606 Fragments arranged by size: 4807 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 AvaI C'yCGr_G Cuts at: 1870 1943 2234 4293 4610 1870 Size: 73 291 2059 317 4545 Fragments arranged by size: 4545 2059 317 291 73 BamHI G'GATC_C Cuts at: 4036 4615 4982 4036 Size: 579 367 6339 Fragments arranged by size: 6339 579 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4385 4606 6061 618 Size: 325 217 627 2598 221 1455 1842 Fragments arranged by size: 2598 1842 1455 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2162 4095 4294 4301 4604 1391 Size: 771 1933 199 7 303 4072 Fragments arranged by size: 4072 1933 771 303 199 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6314 BbvCI CC'TCA_GC Cuts at: 4245 4245 Size: 7285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5476 5510 1903 Size: 34 3539 34 3678 Fragments arranged by size: 3678 3539 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5177 6387 5177 Size: 1210 6075 BclI T'GATC_A Cuts at: 1548 1548 Size: 7285 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 2157 5896 Size: 107 122 71 631 605 382 103 3739 Cuts at: 5896 136 Size: 1525 Fragments arranged by size: 3739 1525 631 605 382 122 107 103 71 BglII A'GATC_T Cuts at: 7280 7280 Size: 7285 BlpI GC'TnA_GC Cuts at: 4353 4702 4353 Size: 349 6936 Bme1580I G_kGCm'C Cuts at: 1790 2162 4390 5346 6502 1790 Size: 372 2228 956 1156 2573 Fragments arranged by size: 2573 2228 1156 956 372 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3685 5016 5974 Size: 416 53 83 399 24 2268 1331 958 Cuts at: 5974 442 Size: 1753 Fragments arranged by size: 2268 1753 1331 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7285 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5945 1239 Size: 4706 2579 Bpu10I CC'TnA_GC Cuts at: 4245 4245 Size: 7285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2475 2577 5410 6275 6573 2475 Size: 102 2833 865 298 3187 Fragments arranged by size: 3187 2833 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2890 5948 882 Size: 617 1391 3058 2219 Fragments arranged by size: 3058 2219 1391 617 BsaAI yAC'GTr Cuts at: 493 1204 4582 5076 493 Size: 711 3378 494 2702 Fragments arranged by size: 3378 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3579 7279 3579 Size: 3700 3585 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2344 5092 5474 6109 Size: 53 83 186 936 811 2748 382 635 Cuts at: 6109 275 Size: 1451 Fragments arranged by size: 2748 1451 936 811 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2335 2580 3171 5718 6116 6390 6537 6948 2335 Size: 245 591 2547 398 274 147 411 2672 Fragments arranged by size: 2672 2547 591 411 398 274 245 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3003 3033 1178 Size: 30 165 30 1600 30 5430 Fragments arranged by size: 5430 1600 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7285 BseYI C'CCAG_C Cuts at: 2161 4444 6488 6984 2161 Size: 2283 2044 496 2462 Fragments arranged by size: 2462 2283 2044 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4257 4542 1163 Size: 573 2521 285 3906 Fragments arranged by size: 3906 2521 573 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4735 5496 6524 1540 Size: 275 186 2734 761 1028 2301 Fragments arranged by size: 2734 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4095 4465 5346 5431 6502 4095 Size: 370 881 85 1071 4878 Fragments arranged by size: 4878 1071 881 370 85 BsmI GAATG_Cn' Cuts at: 4796 4889 4796 Size: 93 7192 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 4095 4294 4301 4390 4465 4604 Size: 399 372 1933 199 7 89 75 139 Cuts at: 4604 5346 5431 6502 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1933 1071 742 399 372 199 139 89 85 75 7 BspEI T'CCGG_A Cuts at: 2335 6948 2335 Size: 4613 2672 BspHI T'CATG_A Cuts at: 2575 5174 6904 2575 Size: 2599 1730 2956 Fragments arranged by size: 2956 2599 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4640 844 Size: 3796 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 5172 1394 Size: 429 2023 1326 3507 Fragments arranged by size: 3507 2023 1326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5780 5954 65 Size: 1183 4532 174 1396 Fragments arranged by size: 4532 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2276 2355 3171 5929 6116 1700 Size: 110 466 79 816 2758 187 2869 Fragments arranged by size: 2869 2758 816 466 187 110 79 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7285 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7285 BssSI C'ACGA_G Cuts at: 3736 3850 5345 6357 3736 Size: 114 1495 1012 4664 Fragments arranged by size: 4664 1495 1012 114 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7285 BstBI TT'CG_AA Cuts at: 2007 6089 2007 Size: 4082 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7285 BstYI r'GATC_y Cuts at: 1972 3478 4036 4615 4982 5367 5384 6836 6922 Size: 1506 558 579 367 385 17 1452 86 Cuts at: 6922 6934 7280 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1506 1452 579 558 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4524 4524 Size: 7285 Bsu36I CC'TnA_GG Cuts at: 2271 2502 2271 Size: 231 7054 BtgI C'CryG_G Cuts at: 513 1065 2049 2145 2219 2310 3015 513 Size: 552 984 96 74 91 705 4783 Fragments arranged by size: 4783 984 705 552 96 91 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6546 Fragments arranged by size: 6546 683 56 BtsI GCAGTG_nn' Cuts at: 2672 3795 4240 4801 5595 5615 2672 Size: 1123 445 561 794 20 4342 Fragments arranged by size: 4342 1123 794 561 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7285 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7250 DraI TTT'AAA Cuts at: 4589 4589 Size: 7285 DraIII CAC_nnn'GTG Cuts at: 4550 4550 Size: 7285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3218 6292 809 Size: 507 1902 3074 1802 Fragments arranged by size: 3074 1902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2403 3081 Size: 54 1425 50 275 18 303 270 678 Cuts at: 3081 3247 4195 4206 4732 5621 7250 8 Size: 166 948 11 526 889 1629 43 Fragments arranged by size: 1629 1425 948 889 678 526 303 275 270 166 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 4732 1537 Size: 275 2920 4090 Fragments arranged by size: 4090 2920 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 4100 4106 4415 5215 1353 Size: 1080 1667 6 309 800 3423 Fragments arranged by size: 3423 1667 1080 800 309 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2261 5872 6246 6392 387 Size: 1064 138 672 3611 374 146 1280 Fragments arranged by size: 3611 1280 1064 672 374 146 138 EcoICRI GAG'CTC Cuts at: 4093 4093 Size: 7285 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2547 3441 3530 3798 3932 5348 5945 Size: 393 915 894 89 268 134 1416 597 Cuts at: 5945 6732 7000 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1416 915 894 787 597 393 268 268 134 89 EcoNI CCTnn'n_nnAGG Cuts at: 3248 3494 4001 3248 Size: 246 507 6532 Fragments arranged by size: 6532 507 246 EcoO109I rG'GnC_Cy Cuts at: 2239 2267 2239 Size: 28 7257 EcoRI G'AATT_C Cuts at: 4594 4972 4594 Size: 378 6907 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3371 3560 3371 Size: 189 7096 FseI GG_CCGG'CC Cuts at: 2280 2280 Size: 7285 FspI TGC'GCA Cuts at: 5791 5791 Size: 7285 HaeII r_GCGC'y Cuts at: 1678 6432 1678 Size: 4754 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2575 2607 5926 Size: 32 135 32 536 32 435 32 3319 Cuts at: 5926 5958 6000 6032 1373 Size: 32 42 32 2626 Fragments arranged by size: 3319 2626 536 435 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4629 4875 669 Size: 988 281 2691 246 3079 Fragments arranged by size: 3079 2691 988 281 246 HindIII A'AGCT_T Cuts at: 748 2398 2471 748 Size: 1650 73 5562 Fragments arranged by size: 5562 1650 73 HpaI GTT'AAC Cuts at: 4875 4875 Size: 7285 KpnI G_GTAC'C Cuts at: 4610 4610 Size: 7285 MfeI C'AATT_G Cuts at: 4884 4884 Size: 7285 MluI A'CGCG_T Cuts at: 7161 7161 Size: 7285 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2327 2667 2752 3534 6399 6583 1305 Size: 1022 340 85 782 2865 184 2007 Fragments arranged by size: 2865 2007 1022 782 340 184 85 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 3083 3249 4208 10 Size: 54 1425 343 573 678 166 959 3087 Fragments arranged by size: 3087 1425 959 678 573 343 166 54 MslI CAynn'nnrTG Cuts at: 518 1166 5243 5602 5761 518 Size: 648 4077 359 159 2042 Fragments arranged by size: 4077 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2069 2147 2221 2707 4701 5378 6526 Size: 445 18 78 74 486 1994 677 1148 Cuts at: 6526 6771 1606 Size: 245 2120 Fragments arranged by size: 2120 1994 1148 677 486 445 245 78 74 18 NaeI GCC'GGC Cuts at: 2278 2357 2278 Size: 79 7206 NcoI C'CATG_G Cuts at: 513 1065 2310 3015 513 Size: 552 1245 705 4783 Fragments arranged by size: 4783 1245 705 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7285 NgoMIV G'CCGG_C Cuts at: 2276 2355 2276 Size: 79 7206 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7285 NotI GC'GGCC_GC Cuts at: 4732 4732 Size: 7285 NspI r_CATG'y Cuts at: 1132 1229 3290 4064 4579 4643 6188 1132 Size: 97 2061 774 515 64 1545 2229 Fragments arranged by size: 2229 2061 1545 774 515 97 64 PasI CC'CwG_GG Cuts at: 1348 2096 3901 4287 1348 Size: 748 1805 386 4346 Fragments arranged by size: 4346 1805 748 386 PciI A'CATG_T Cuts at: 6184 6184 Size: 7285 PflMI CCAn_nnn'nTGG Cuts at: 1108 3256 3982 4510 1108 Size: 2148 726 528 3883 Fragments arranged by size: 3883 2148 726 528 PmeI GTTT'AAAC Cuts at: 4589 4589 Size: 7285 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5354 5386 6867 6899 5354 Size: 32 1481 32 5740 Fragments arranged by size: 5740 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7285 PsiI TTA'TAA Cuts at: 2748 3870 4855 2748 Size: 1122 985 5178 Fragments arranged by size: 5178 1122 985 PspOMI G'GGCC_C Cuts at: 2158 2158 Size: 7285 PstI C_TGCA'G Cuts at: 830 2068 2679 3973 4321 4637 830 Size: 1238 611 1294 348 316 3478 Fragments arranged by size: 3478 1294 1238 611 348 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7285 PvuII CAG'CTG Cuts at: 2707 2707 Size: 7285 SacI G_AGCT'C Cuts at: 4095 4095 Size: 7285 SacII CC_GC'GG Cuts at: 2052 2148 2222 2052 Size: 96 74 7115 Fragments arranged by size: 7115 96 74 SalI G'TCGA_C Cuts at: 1936 4627 1936 Size: 2691 4594 SapI GCTCTTCn'nnn_ Cuts at: 4100 4100 Size: 7285 SbfI CC_TGCA'GG Cuts at: 4637 4637 Size: 7285 ScaI AGT'ACT Cuts at: 5533 7245 5533 Size: 1712 5573 SexAI A'CCwGG_T Cuts at: 4546 4546 Size: 7285 SfcI C'TryA_G Cuts at: 826 1045 2064 2675 3969 4317 4633 5768 6449 Size: 219 1019 611 1294 348 316 1135 681 Cuts at: 6449 6640 826 Size: 191 1471 Fragments arranged by size: 1471 1294 1135 1019 681 611 348 316 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2157 2157 Size: 7285 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7285 SmaI CCC'GGG Cuts at: 1872 2236 4612 1872 Size: 364 2376 4545 Fragments arranged by size: 4545 2376 364 SmlI C'TyrA_G Cuts at: 820 1017 1943 2490 2592 3668 5389 6290 6552 Size: 197 926 547 102 1076 1721 901 262 Cuts at: 6552 820 Size: 1553 Fragments arranged by size: 1721 1553 1076 926 901 547 262 197 102 SnaBI TAC'GTA Cuts at: 493 4582 5076 493 Size: 4089 494 2702 Fragments arranged by size: 4089 2702 494 SpeI A'CTAG_T Cuts at: 152 4138 152 Size: 3986 3299 SphI G_CATG'C Cuts at: 1132 4643 1132 Size: 3511 3774 SspI AAT'ATT Cuts at: 5 52 5209 5 Size: 47 5157 2081 Fragments arranged by size: 5157 2081 47 StuI AGG'CCT Cuts at: 1517 3765 1517 Size: 2248 5037 StyI C'CwwG_G Cuts at: 513 1065 2310 3015 3244 4724 513 Size: 552 1245 705 229 1480 3074 Fragments arranged by size: 3074 1480 1245 705 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2951 3448 3893 5312 5329 5482 5667 Size: 1255 1371 497 445 1419 17 153 185 Cuts at: 5667 6112 325 Size: 445 1498 Fragments arranged by size: 1498 1419 1371 1255 497 445 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 5531 7243 Size: 274 80 33 51 1443 402 3152 1712 Cuts at: 7243 96 Size: 138 Fragments arranged by size: 3152 1712 1443 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3003 3035 3810 3842 7245 7277 3003 Size: 32 775 32 3403 32 3011 Fragments arranged by size: 3403 3011 775 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7285 XbaI T'CTAG_A Cuts at: 4621 4621 Size: 7285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2956 4258 2956 Size: 1302 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7285 XmaI C'CCGG_G Cuts at: 1870 2234 4610 1870 Size: 364 2376 4545 Fragments arranged by size: 4545 2376 364 XmnI GAAnn'nnTTC Cuts at: 3543 5414 3543 Size: 1871 5414 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5093 276 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI SrfI SwaI