Restriction map for
pFN21ASDA0592
(Circular) (Six-base) MAPSORT of: pFN21ASDA0592.seq Check: 3721 from: 1 to: 8689
pFN21ASDA0592 8689 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6499 278
Size: 53 83 186 936 4963 2468
Fragments arranged by size:
4963 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5074 6032 1937
Size: 3137 958 4594
Fragments arranged by size:
4594 3137 958
Acc65I G'GTAC_C
Cuts at: 6010 6010
Size: 8689
AclI AA'CG_TT
Cuts at: 1563 6816 7189 1563
Size: 5253 373 3063
Fragments arranged by size:
5253 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3003 3323 4911 6752 8136 8404 1632
Size: 1371 320 1588 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1588 1384 1371 320 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8492
AflIII A'CryG_T
Cuts at: 1203 7588 8565 1203
Size: 6385 977 1327
Fragments arranged by size:
6385 1327 977
AgeI A'CCGG_T
Cuts at: 7520 7520
Size: 8689
AhdI GACnn_n'nnGTC
Cuts at: 3940 7418 3940
Size: 3478 5211
AleI CACnn'nnGTG
Cuts at: 1166 3272 1166
Size: 2106 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5103 1184
Size: 3919 4770
AlwNI CAG_nnn'CTG
Cuts at: 2053 3245 6432 8004 2053
Size: 1192 3187 1572 2738
Fragments arranged by size:
3187 2738 1572 1192
ApaLI G'TGCA_C
Cuts at: 6746 7902 6746
Size: 1156 7533
ApoI r'AATT_y
Cuts at: 1493 2168 4283 4837 5998 6224 6376 8485 1493
Size: 675 2115 554 1161 226 152 2109 1697
Fragments arranged by size:
2115 2109 1697 1161 675 554 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7243 160
Size: 872 6211 1606
Fragments arranged by size:
6211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8689
AvaI C'yCGr_G
Cuts at: 1870 1943 4976 6014 1870
Size: 73 3033 1038 4545
Fragments arranged by size:
4545 3033 1038 73
BamHI G'GATC_C
Cuts at: 6019 6386 6019
Size: 367 8322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3415 4186 4613 5174
Size: 325 217 627 269 1359 771 427 561
Cuts at: 5174 6010 7465 618
Size: 836 1455 1842
Fragments arranged by size:
1842 1455 1359 836 771 627 561 427
325 269 217
BanII G_rGCy'C
Cuts at: 1391 2073 3846 5468 6008 1391
Size: 682 1773 1622 540 4072
Fragments arranged by size:
4072 1773 1622 682 540
BbeI G_GCGC'C
Cuts at: 5178 5178
Size: 8689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2260 2387 2990 3198 4029 4076 4104
Size: 971 361 127 603 208 831 47 28
Cuts at: 4104 4531 5232 928
Size: 427 701 4385
Fragments arranged by size:
4385 971 831 701 603 427 361 208
127 47 28
BbvCI CC'TCA_GC
Cuts at: 2355 5127 2355
Size: 2772 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4496 4530 6880 6914 1903
Size: 34 2559 34 2350 34 3678
Fragments arranged by size:
3678 2559 2350 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5876 6581 7791 5876
Size: 705 1210 6774
Fragments arranged by size:
6774 1210 705
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7300 136
Size: 107 122 71 631 605 5628 1525
Fragments arranged by size:
5628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2404 3487 4334 4884 8684 2404
Size: 1083 847 550 3800 2409
Fragments arranged by size:
3800 2409 1083 847 550
BlpI GC'TnA_GC
Cuts at: 6106 6106
Size: 8689
Bme1580I G_kGCm'C
Cuts at: 1790 5378 6750 7906 1790
Size: 3588 1372 1156 2573
Fragments arranged by size:
3588 2573 1372 1156
BmgBI CAC'GTC
Cuts at: 1318 3726 1318
Size: 2408 6281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 6420 7378
Size: 416 53 83 399 24 876 4127 958
Cuts at: 7378 442
Size: 1753
Fragments arranged by size:
4127 1753 958 876 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 3667 4103 4195 3091
Size: 576 436 92 7585
Fragments arranged by size:
7585 576 436 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3111 3204 3959 5307 7349 1239
Size: 1872 93 755 1348 2042 2579
Fragments arranged by size:
2579 2042 1872 1348 755 93
Bpu10I CC'TnA_GC
Cuts at: 2355 3321 4341 5016 5109 5127 2355
Size: 966 1020 675 93 18 5917
Fragments arranged by size:
5917 1020 966 675 93 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3581 6814 7679 7977 2333
Size: 1248 3233 865 298 3045
Fragments arranged by size:
3233 3045 1248 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7352 882
Size: 617 5853 2219
Fragments arranged by size:
5853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5986 6480 493
Size: 711 4782 494 2702
Fragments arranged by size:
4782 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4677 5505 8683 4677
Size: 828 3178 4683
Fragments arranged by size:
4683 3178 828
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 5175 6496 6878
Size: 53 83 186 936 530 3112 1321 382
Cuts at: 6878 7513 275
Size: 635 1451
Fragments arranged by size:
3112 1451 1321 936 635 530 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2031 7122 7520 7794 7941 8352 2031
Size: 5091 398 274 147 411 2368
Fragments arranged by size:
5091 2368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2072 2090 2102 2483
Size: 30 165 30 657 12 18 12 381
Cuts at: 2483 2513 3177 3207 3881 3911 4936 4966 5840
Size: 30 664 30 674 30 1025 30 874
Cuts at: 5840 5870 1178
Size: 30 3997
Fragments arranged by size:
3997 1025 874 674 664 657 381 165
30 30 30 30 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2351 2717 2720 2840 2957 3155 3215
Size: 928 237 366 3 120 117 198 60
Cuts at: 3215 3659 3902 4079 4125 5252 5848 1186
Size: 444 243 177 46 1127 596 4027
Fragments arranged by size:
4027 1127 928 596 444 366 243 237
198 177 120 117 60 46 3
BseYI C'CCAG_C
Cuts at: 3235 4701 4852 7892 8388 3235
Size: 1466 151 3040 496 3536
Fragments arranged by size:
3536 3040 1466 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5109 5244 5886 1163
Size: 573 3373 135 642 3966
Fragments arranged by size:
3966 3373 642 573 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2087 2139 6139 6900 7928 1540
Size: 275 186 86 52 4000 761 1028 2301
Fragments arranged by size:
4000 2301 1028 761 275 186 86 52
BsiHKAI G_wGCw'C
Cuts at: 3846 4532 6750 6835 7906 3846
Size: 686 2218 85 1071 4629
Fragments arranged by size:
4629 2218 1071 686 85
BsmI GAATG_Cn'
Cuts at: 4357 5971 6200 6293 4357
Size: 1614 229 93 6753
Fragments arranged by size:
6753 1614 229 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 3846 4532 5378 5468 6008 6750
Size: 399 283 1773 686 846 90 540 742
Cuts at: 6750 6835 7906 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1773 1071 846 742 686 540 399
283 90 85
BspEI T'CCGG_A
Cuts at: 8352 8352
Size: 8689
BspHI T'CATG_A
Cuts at: 6578 8308 6578
Size: 1730 6959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3930 6044 844
Size: 3086 2114 3489
Fragments arranged by size:
3489 3086 2114
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 6576 1394
Size: 429 260 4493 3507
Fragments arranged by size:
4493 3507 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4047 5154 7184 7358 65
Size: 1183 2799 1107 2030 174 1396
Fragments arranged by size:
2799 2030 1396 1183 1107 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 4991 7333 7520 1700
Size: 110 249 2932 2342 187 2869
Fragments arranged by size:
2932 2869 2342 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8689
BssSI C'ACGA_G
Cuts at: 2385 6749 7761 2385
Size: 4364 1012 3313
Fragments arranged by size:
4364 3313 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8689
BstBI TT'CG_AA
Cuts at: 2007 7493 2007
Size: 5486 3203
BstEII G'GTnAC_C
Cuts at: 1184 3571 1184
Size: 2387 6302
BstYI r'GATC_y
Cuts at: 1972 2103 2404 2551 3487 4334 4884 5381 6019
Size: 131 301 147 936 847 550 497 638
Cuts at: 6019 6386 6771 6788 8240 8326 8338 8684 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 936 847 638 550 497 385
367 346 301 147 131 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2962 4830 2962
Size: 1868 6821
BtgI C'CryG_G
Cuts at: 513 1065 2087 2925 4385 4948 5370 5391 513
Size: 552 1022 838 1460 563 422 21 3811
Fragments arranged by size:
3811 1460 1022 838 563 552 422 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3498 4221 487
Size: 683 56 2272 723 4955
Fragments arranged by size:
4955 2272 723 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3456 3886 5503 6205 6999 7019 2665
Size: 791 430 1617 702 794 20 4335
Fragments arranged by size:
4335 1617 794 791 702 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8654
DraI TTT'AAA
Cuts at: 5993 5993
Size: 8689
DraIII CAC_nnn'GTG
Cuts at: 5938 5938
Size: 8689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7696 809
Size: 507 6380 1802
Fragments arranged by size:
6380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 2136 5191
Size: 54 1425 50 275 18 254 52 3055
Cuts at: 5191 5198 6136 7025 8654 8
Size: 7 938 889 1629 43
Fragments arranged by size:
3055 1629 1425 938 889 275 254 54
52 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2084 2136 6136 1537
Size: 275 272 52 4000 4090
Fragments arranged by size:
4090 4000 275 272 52
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 2694 2775 3024 3158 3750 3816 4209
Size: 1332 9 81 249 134 592 66 393
Cuts at: 4209 4437 4545 6619 1353
Size: 228 108 2074 3423
Fragments arranged by size:
3423 2074 1332 592 393 249 228 134
108 81 66 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7276 7650 7796 387
Size: 1064 138 5687 374 146 1280
Fragments arranged by size:
5687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3844 3844
Size: 8689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3003 3111 3204 3323 3959 4911 5307
Size: 393 1371 108 93 119 636 952 396
Cuts at: 5307 6752 7349 8136 8404 1239
Size: 1445 597 787 268 1524
Fragments arranged by size:
1524 1445 1371 952 787 636 597 396
393 268 119 108 93
EcoNI CCTnn'n_nnAGG
Cuts at: 5268 5481 5268
Size: 213 8476
EcoO109I rG'GnC_Cy
Cuts at: 2193 3969 5150 5242 2193
Size: 1776 1181 92 5640
Fragments arranged by size:
5640 1776 1181 92
EcoRI G'AATT_C
Cuts at: 2168 5998 6376 2168
Size: 3830 378 4481
Fragments arranged by size:
4481 3830 378
EcoRV GAT'ATC
Cuts at: 2479 3466 5764 2479
Size: 987 2298 5404
Fragments arranged by size:
5404 2298 987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3592 4055 4574 3592
Size: 463 519 7707
Fragments arranged by size:
7707 519 463
FspI TGC'GCA
Cuts at: 7195 7195
Size: 8689
HaeII r_GCGC'y
Cuts at: 1678 5178 7836 1678
Size: 3500 2658 2531
Fragments arranged by size:
3500 2658 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 3194 3226 3395
Size: 32 135 32 1121 32 469 32 169
Cuts at: 3395 3427 3787 3819 3926 3958 4936 4968 4990
Size: 32 360 32 107 32 978 32 22
Cuts at: 4990 5022 5603 5635 5840 5872 7330 7362 7404
Size: 32 581 32 205 32 1458 32 42
Cuts at: 7404 7436 1373
Size: 32 2626
Fragments arranged by size:
2626 1458 1121 978 581 469 360 205
169 135 107 42 32 32 32 32
32 32 32 32 32 32 32 32
32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 4600 4753 6033 6279 669
Size: 988 281 2662 153 1280 246 3079
Fragments arranged by size:
3079 2662 1280 988 281 246 153
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8689
HpaI GTT'AAC
Cuts at: 4753 6279 4753
Size: 1526 7163
KasI G'GCGC_C
Cuts at: 5174 5174
Size: 8689
KpnI G_GTAC'C
Cuts at: 6014 6014
Size: 8689
MfeI C'AATT_G
Cuts at: 6288 6288
Size: 8689
MluI A'CGCG_T
Cuts at: 8565 8565
Size: 8689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 5967 7803 7987 1305
Size: 741 3921 1836 184 2007
Fragments arranged by size:
3921 2007 1836 741 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5193 10
Size: 54 1425 343 3361 3506
Fragments arranged by size:
3506 3361 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 2765 3272 4384 6647 7006 7165
Size: 648 1567 32 507 1112 2263 359 159
Cuts at: 7165 518
Size: 2042
Fragments arranged by size:
2263 2042 1567 1112 648 507 359 159
32
MspA1I CmG'CkG
Cuts at: 1606 3782 4856 4874 5252 5258 6105 6782 7930
Size: 2176 1074 18 378 6 847 677 1148
Cuts at: 7930 8175 1606
Size: 245 2120
Fragments arranged by size:
2176 2120 1148 1074 847 677 378 245
18 6
NaeI GCC'GGC
Cuts at: 2061 2061
Size: 8689
NarI GG'CG_CC
Cuts at: 5175 5175
Size: 8689
NcoI C'CATG_G
Cuts at: 513 1065 2925 4385 513
Size: 552 1860 1460 4817
Fragments arranged by size:
4817 1860 1460 552
NdeI CA'TA_TG
Cuts at: 387 4811 387
Size: 4424 4265
NgoMIV G'CCGG_C
Cuts at: 2059 2059
Size: 8689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8689
NotI GC'GGCC_GC
Cuts at: 6136 6136
Size: 8689
NspI r_CATG'y
Cuts at: 1132 1229 6047 7592 1132
Size: 97 4818 1545 2229
Fragments arranged by size:
4818 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3957 1348
Size: 2609 6080
PciI A'CATG_T
Cuts at: 7588 7588
Size: 8689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2053 4646 4708 4954 5450 1108
Size: 945 2593 62 246 496 4347
Fragments arranged by size:
4347 2593 945 496 246 62
PfoI T'CCnGG_A
Cuts at: 2229 2421 4542 5562 2229
Size: 192 2121 1020 5356
Fragments arranged by size:
5356 2121 1020 192
PmeI GTTT'AAAC
Cuts at: 5993 5993
Size: 8689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6758 6790 8271 8303 6758
Size: 32 1481 32 7144
Fragments arranged by size:
7144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2193 3969 5150 2193
Size: 1776 1181 5732
Fragments arranged by size:
5732 1776 1181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8689
PsiI TTA'TAA
Cuts at: 6259 6259
Size: 8689
PstI C_TGCA'G
Cuts at: 830 6041 830
Size: 5211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8689
PvuII CAG'CTG
Cuts at: 3782 5252 3782
Size: 1470 7219
SacI G_AGCT'C
Cuts at: 3846 3846
Size: 8689
SalI G'TCGA_C
Cuts at: 1936 6031 1936
Size: 4095 4594
SanDI GG'GwC_CC
Cuts at: 3969 3969
Size: 8689
SapI GCTCTTCn'nnn_
Cuts at: 4545 4545
Size: 8689
SbfI CC_TGCA'GG
Cuts at: 6041 6041
Size: 8689
ScaI AGT'ACT
Cuts at: 2346 6937 8649 2346
Size: 4591 1712 2386
Fragments arranged by size:
4591 2386 1712
SfcI C'TryA_G
Cuts at: 826 1045 2153 2162 2652 6037 7172 7853 8044
Size: 219 1108 9 490 3385 1135 681 191
Cuts at: 8044 826
Size: 1471
Fragments arranged by size:
3385 1471 1135 1108 681 490 219 191
9
SfoI GGC'GCC
Cuts at: 5176 5176
Size: 8689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8689
SmaI CCC'GGG
Cuts at: 1872 4978 6016 1872
Size: 3106 1038 4545
Fragments arranged by size:
4545 3106 1038
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3596 6793 7694 7956 820
Size: 197 926 405 1248 3197 901 262 1553
Fragments arranged by size:
3197 1553 1248 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 5986 6480 493
Size: 5493 494 2702
Fragments arranged by size:
5493 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8689
SphI G_CATG'C
Cuts at: 1132 6047 1132
Size: 4915 3774
SspI AAT'ATT
Cuts at: 5 52 3904 6613 5
Size: 47 3852 2709 2081
Fragments arranged by size:
3852 2709 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8689
StyI C'CwwG_G
Cuts at: 513 1065 2925 3335 3990 4053 4269 4385 5264
Size: 552 1860 410 655 63 216 116 879
Cuts at: 5264 5409 6128 513
Size: 145 719 3074
Fragments arranged by size:
3074 1860 879 719 655 552 410 216
145 116 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 5438 6716 6733 6886 7071 7516
Size: 1255 1497 2361 1278 17 153 185 445
Cuts at: 7516 325
Size: 1498
Fragments arranged by size:
2361 1498 1497 1278 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2344 6935 8647
Size: 274 80 33 51 1443 367 4591 1712
Cuts at: 8647 96
Size: 138
Fragments arranged by size:
4591 1712 1443 367 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2072 2092 2104 3432 3464 3881 3913 4132
Size: 12 20 12 1328 32 417 32 219
Cuts at: 4132 4164 5924 5956 8649 8681 2060
Size: 32 1760 32 2693 32 2068
Fragments arranged by size:
2693 2068 1760 1328 417 219 32 32
32 32 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 3799 1316
Size: 2483 6206
XbaI T'CTAG_A
Cuts at: 6025 6025
Size: 8689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2932 5443 5809 2932
Size: 2511 366 5812
Fragments arranged by size:
5812 2511 366
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8689
XmaI C'CCGG_G
Cuts at: 1870 4976 6014 1870
Size: 3106 1038 4545
Fragments arranged by size:
4545 3106 1038
XmnI GAAnn'nnTTC
Cuts at: 3016 4785 6818 3016
Size: 1769 2033 4887
Fragments arranged by size:
4887 2033 1769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6497 276
Size: 53 83 186 936 4963 2468
Fragments arranged by size:
4963 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstXI BstZ17I FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI
SfiI SrfI SwaI