Restriction map for
pFN21ASDA0582
(Circular) (Six-base) MAPSORT of: pFN21ASDA0582.seq Check: 2148 from: 1 to: 7000
pFN21ASDA0582 7000 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4810 278
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3024 4343 1937
Size: 1087 1319 4594
Fragments arranged by size:
4594 1319 1087
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 4085 5063 6447 6715 1632
Size: 450 2003 978 1384 268 1917
Fragments arranged by size:
2003 1917 1384 978 450 268
AflII C'TTAA_G
Cuts at: 820 1017 3146 820
Size: 197 2129 4674
Fragments arranged by size:
4674 2129 197
AflIII A'CryG_T
Cuts at: 1203 5899 6876 1203
Size: 4696 977 1327
Fragments arranged by size:
4696 1327 977
AgeI A'CCGG_T
Cuts at: 3816 5831 3816
Size: 2015 4985
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 4281 1166
Size: 3115 3885
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7000
AlwNI CAG_nnn'CTG
Cuts at: 2425 2512 4063 4108 4743 6315 2425
Size: 87 1551 45 635 1572 3110
Fragments arranged by size:
3110 1572 1551 635 87 45
ApaI G_GGCC'C
Cuts at: 2130 3132 2130
Size: 1002 5998
ApaLI G'TGCA_C
Cuts at: 5057 6213 5057
Size: 1156 5844
ApoI r'AATT_y
Cuts at: 1493 4309 4535 4687 6796 1493
Size: 2816 226 152 2109 1697
Fragments arranged by size:
2816 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 3761 4325 1870
Size: 73 1011 807 564 4545
Fragments arranged by size:
4545 1011 807 564 73
BamHI G'GATC_C
Cuts at: 3521 3670 4330 4697 3521
Size: 149 660 367 5824
Fragments arranged by size:
5824 660 367 149
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3405 4321 5776 618
Size: 325 217 627 1618 916 1455 1842
Fragments arranged by size:
1842 1618 1455 916 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 2175 2257 2292 2582 3132 3232 3326
Size: 739 45 82 35 290 550 100 94
Cuts at: 3326 3575 4319 1391
Size: 249 744 4072
Fragments arranged by size:
4072 744 739 550 290 249 100 94
82 45 35
BbeI G_GCGC'C
Cuts at: 3409 3409
Size: 7000
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 2393 3911 4002 928
Size: 971 171 323 1518 91 3926
Fragments arranged by size:
3926 1518 971 323 171 91
BbvCI CC'TCA_GC
Cuts at: 2163 2519 2961 2163
Size: 356 442 6202
Fragments arranged by size:
6202 442 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3452 3486 5191 5225 1903
Size: 34 1515 34 1705 34 3678
Fragments arranged by size:
3678 1705 1515 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2196 4892 6102 2196
Size: 2696 1210 3094
Fragments arranged by size:
3094 2696 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5611 136
Size: 107 122 71 631 605 3939 1525
Fragments arranged by size:
3939 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2923 6995 2923
Size: 4072 2928
BlpI GC'TnA_GC
Cuts at: 2576 2595 4417 2576
Size: 19 1822 5159
Fragments arranged by size:
5159 1822 19
Bme1580I G_kGCm'C
Cuts at: 1790 2130 3132 3403 3718 5061 6217 1790
Size: 340 1002 271 315 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 1002 340 315 271
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 2755 2851
Size: 416 53 83 399 24 654 684 96
Cuts at: 2851 3561 4118 4731 5689 442
Size: 710 557 613 958 1753
Fragments arranged by size:
1753 958 710 684 654 613 557 416
399 96 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3671
Size: 7000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 2627 2726 3033 3145 3607 3666 4193
Size: 1131 257 99 307 112 462 59 527
Cuts at: 4193 5660 1239
Size: 1467 2579
Fragments arranged by size:
2579 1467 1131 527 462 307 257 112
99 59
Bpu10I CC'TnA_GC
Cuts at: 2163 2455 2519 2539 2961 3335 2163
Size: 292 64 20 422 374 5828
Fragments arranged by size:
5828 422 374 292 64 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3989 5125 5990 6288 3989
Size: 1136 865 298 4701
Fragments arranged by size:
4701 1136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2346 2549 2698 3772 5663 882
Size: 617 847 203 149 1074 1891 2219
Fragments arranged by size:
2219 1891 1074 847 617 203 149
BsaAI yAC'GTr
Cuts at: 493 1204 4297 4791 493
Size: 711 3093 494 2702
Fragments arranged by size:
3093 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4053 6994 4053
Size: 2941 4059
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2818 3406 4807 5189
Size: 53 83 186 936 1285 588 1401 382
Cuts at: 5189 5824 275
Size: 635 1451
Fragments arranged by size:
1451 1401 1285 936 635 588 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3816 5433 5831 6105 6252 6663 3816
Size: 1617 398 274 147 411 4153
Fragments arranged by size:
4153 1617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2873 2903 1178
Size: 30 165 30 1470 30 5275
Fragments arranged by size:
5275 1470 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 2686 2689 3760 3882 1186
Size: 1237 263 3 1071 122 4304
Fragments arranged by size:
4304 1237 1071 263 122 3
BseYI C'CCAG_C
Cuts at: 3330 6203 6699 3330
Size: 2873 496 3631
Fragments arranged by size:
3631 2873 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2298 3400 4219 1163
Size: 573 562 1102 819 3944
Fragments arranged by size:
3944 1102 819 573 562
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2715 2884 4450 5211 6239 1540
Size: 275 186 714 169 1566 761 1028 2301
Fragments arranged by size:
2301 1566 1028 761 714 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 4278 5061 5146 6217 4278
Size: 783 85 1071 5061
Fragments arranged by size:
5061 1071 783 85
BsmI GAATG_Cn'
Cuts at: 4511 4604 4511
Size: 93 6907
BsmBI CGTCTCn'nnnn_
Cuts at: 2809 2809
Size: 7000
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2175 2257 2292 2582 3132 3232
Size: 399 340 45 82 35 290 550 100
Cuts at: 3232 3326 3403 3575 3718 4278 4319 5061 5146
Size: 94 77 172 143 560 41 742 85
Cuts at: 5146 6217 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 560 550 399 340 290
172 143 100 94 85 82 77 45
41 35
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 4889 6619 4889
Size: 1730 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 3422 4355 844
Size: 1371 1207 933 3489
Fragments arranged by size:
3489 1371 1207 933
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 3482 4887 1394
Size: 429 284 1375 1405 3507
Fragments arranged by size:
3507 1405 1375 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4275 5495 5669 65
Size: 1183 2654 373 1220 174 1396
Fragments arranged by size:
2654 1396 1220 1183 373 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2710 3291 3816 4026 5644 5831 1700
Size: 110 900 581 525 210 1618 187 2869
Fragments arranged by size:
2869 1618 900 581 525 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 2670 5060 6072 2670
Size: 2390 1012 3598
Fragments arranged by size:
3598 2390 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7000
BstBI TT'CG_AA
Cuts at: 2007 5804 2007
Size: 3797 3203
BstEII G'GTnAC_C
Cuts at: 1184 2648 2766 3538 1184
Size: 1464 118 772 4646
Fragments arranged by size:
4646 1464 772 118
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2084 3665 2029
Size: 55 1581 5364
Fragments arranged by size:
5364 1581 55
BstYI r'GATC_y
Cuts at: 1972 2923 3521 3662 3670 4330 4697 5082 5099
Size: 951 598 141 8 660 367 385 17
Cuts at: 5099 6551 6637 6649 6995 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 951 660 598 385 367 346
141 86 17 12 8
Bsu36I CC'TnA_GG
Cuts at: 3275 3675 3275
Size: 400 6600
BtgI C'CryG_G
Cuts at: 513 1065 2022 2340 3970 4260 513
Size: 552 957 318 1630 290 3253
Fragments arranged by size:
3253 1630 957 552 318 290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 3108 3158 4516 5310 5330 3108
Size: 50 1358 794 20 4778
Fragments arranged by size:
4778 1358 794 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 4304 4304
Size: 7000
DraIII CAC_nnn'GTG
Cuts at: 2321 2321
Size: 7000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6007 809
Size: 507 4691 1802
Fragments arranged by size:
4691 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 3821 4447
Size: 54 1425 50 275 18 855 1136 626
Cuts at: 4447 5336 6965 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1136 889 855 626 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4447 1537
Size: 275 2635 4090
Fragments arranged by size:
4090 2635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3377 3435 3921 4930 1353
Size: 2024 58 486 1009 3423
Fragments arranged by size:
3423 2024 1009 486 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2678 5587 5961 6107 387
Size: 1064 138 1089 2909 374 146 1280
Fragments arranged by size:
2909 1280 1089 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2370 2627 2726 3033 3145 3607
Size: 393 450 288 257 99 307 112 462
Cuts at: 3607 3666 4085 4193 5063 5660 6447 6715 1239
Size: 59 419 108 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 597 462 450 419 393
307 288 268 257 112 108 99 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3364 3364
Size: 7000
EcoO109I rG'GnC_Cy
Cuts at: 2126 2750 3757 2126
Size: 624 1007 5369
Fragments arranged by size:
5369 1007 624
EcoRI G'AATT_C
Cuts at: 4309 4687 4309
Size: 378 6622
EcoRV GAT'ATC
Cuts at: 3559 3559
Size: 7000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2945 3869 2945
Size: 924 6076
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 3409 6147 1678
Size: 1731 2738 2531
Fragments arranged by size:
2738 2531 1731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 2471 2503 2873
Size: 32 135 32 588 32 279 32 370
Cuts at: 2873 2905 3023 3055 3308 3340 5641 5673 5715
Size: 32 118 32 253 32 2301 32 42
Cuts at: 5715 5747 1373
Size: 32 2626
Fragments arranged by size:
2626 2301 588 370 279 253 135 118
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4344 4590 669
Size: 988 281 2406 246 3079
Fragments arranged by size:
3079 2406 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2393 748
Size: 1645 5355
HpaI GTT'AAC
Cuts at: 4590 4590
Size: 7000
KasI G'GCGC_C
Cuts at: 3405 3405
Size: 7000
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3792 4092 6114 6298 1305
Size: 2487 300 2022 184 2007
Fragments arranged by size:
2487 2022 2007 300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 3823 10
Size: 54 1425 343 855 1136 3187
Fragments arranged by size:
3187 1425 1136 855 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4281 4958 5317 5476 518
Size: 648 3115 677 359 159 2042
Fragments arranged by size:
3115 2042 677 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2607 2996 3922 4416 5093 6241 6486 1606
Size: 1001 389 926 494 677 1148 245 2120
Fragments arranged by size:
2120 1148 1001 926 677 494 389 245
NarI GG'CG_CC
Cuts at: 3406 3406
Size: 7000
NcoI C'CATG_G
Cuts at: 513 1065 2022 2340 4260 513
Size: 552 957 318 1920 3253
Fragments arranged by size:
3253 1920 957 552 318
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 4225 4358 5903 1132
Size: 97 2996 133 1545 2229
Fragments arranged by size:
2996 2229 1545 133 97
PasI CC'CwG_GG
Cuts at: 1348 2029 2123 1348
Size: 681 94 6225
Fragments arranged by size:
6225 681 94
PciI A'CATG_T
Cuts at: 5899 5899
Size: 7000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2512 3125 1108
Size: 1404 613 4983
Fragments arranged by size:
4983 1404 613
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2569 2601 5069 5101 6582 6614 2569
Size: 32 2468 32 1481 32 2955
Fragments arranged by size:
2955 2468 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2750 2750
Size: 7000
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PspOMI G'GGCC_C
Cuts at: 2126 3128 2126
Size: 1002 5998
PstI C_TGCA'G
Cuts at: 830 2102 2612 3012 3982 4020 4352 830
Size: 1272 510 400 970 38 332 3478
Fragments arranged by size:
3478 1272 970 510 400 332 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 2607 2996 3922 2607
Size: 389 926 5685
Fragments arranged by size:
5685 926 389
SalI G'TCGA_C
Cuts at: 1936 4342 1936
Size: 2406 4594
SapI GCTCTTCn'nnn_
Cuts at: 3921 3921
Size: 7000
SbfI CC_TGCA'GG
Cuts at: 3012 4352 3012
Size: 1340 5660
ScaI AGT'ACT
Cuts at: 2840 5248 6960 2840
Size: 2408 1712 2880
Fragments arranged by size:
2880 2408 1712
SfcI C'TryA_G
Cuts at: 826 1045 2098 2608 3008 3300 3978 4016 4348
Size: 219 1053 510 400 292 678 38 332
Cuts at: 4348 5483 6164 6355 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1053 681 678 510 400 332
292 219 191 38
SfoI GGC'GCC
Cuts at: 3407 3407
Size: 7000
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 2956 4327 1872
Size: 1084 1371 4545
Fragments arranged by size:
4545 1371 1084
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3146 4004 5104 6005 6267 820
Size: 197 926 1203 858 1100 901 262 1553
Fragments arranged by size:
1553 1203 1100 926 901 858 262 197
SnaBI TAC'GTA
Cuts at: 493 4297 4791 493
Size: 3804 494 2702
Fragments arranged by size:
3804 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 4358 1132
Size: 3226 3774
SrfI GCCC'GGGC
Cuts at: 2956 2956
Size: 7000
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7000
StyI C'CwwG_G
Cuts at: 513 1065 2022 2340 2688 3534 3576 3658 4141
Size: 552 957 318 348 846 42 82 483
Cuts at: 4141 4260 4439 513
Size: 119 179 3074
Fragments arranged by size:
3074 957 846 552 483 348 318 179
119 82 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2021 4293 5027 5044 5197 5382 5827
Size: 1255 441 2272 734 17 153 185 445
Cuts at: 5827 325
Size: 1498
Fragments arranged by size:
2272 1498 1255 734 445 441 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2838 5246 6958
Size: 274 80 33 51 1443 861 2408 1712
Cuts at: 6958 96
Size: 138
Fragments arranged by size:
2408 1712 1443 861 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3338 3370 6960 6992 3338
Size: 32 3590 32 3346
Fragments arranged by size:
3590 3346 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3002 1316
Size: 1686 5314
XbaI T'CTAG_A
Cuts at: 2249 4336 2249
Size: 2087 4913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3179 3532 3641 4267 3179
Size: 353 109 626 5912
Fragments arranged by size:
5912 626 353 109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 2954 4325 1870
Size: 1084 1371 4545
Fragments arranged by size:
4545 1371 1084
XmnI GAAnn'nnTTC
Cuts at: 5129 5129
Size: 7000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4808 276
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SwaI