Restriction map for
pFN21ASDA0576
(Circular) (Six-base) MAPSORT of: pFN21ASDA0576.seq Check: 5804 from: 1 to: 7912
pFN21ASDA0576 7912 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4409 4409
Size: 7912
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5722 278
Size: 53 83 186 936 4186 2468
Fragments arranged by size:
4186 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5255 1937
Size: 3318 4594
Acc65I G'GTAC_C
Cuts at: 5233 5233
Size: 7912
AclI AA'CG_TT
Cuts at: 1563 6039 6412 1563
Size: 4476 373 3063
Fragments arranged by size:
4476 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2632 5975 7359 7627 1632
Size: 1000 3343 1384 268 1917
Fragments arranged by size:
3343 1917 1384 1000 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7715
AflIII A'CryG_T
Cuts at: 1203 2505 3667 6811 7788 1203
Size: 1302 1162 3144 977 1327
Fragments arranged by size:
3144 1327 1302 1162 977
AgeI A'CCGG_T
Cuts at: 6743 6743
Size: 7912
AhdI GACnn_n'nnGTC
Cuts at: 2035 3182 6641 2035
Size: 1147 3459 3306
Fragments arranged by size:
3459 3306 1147
AleI CACnn'nnGTG
Cuts at: 1166 2510 3340 1166
Size: 1344 830 5738
Fragments arranged by size:
5738 1344 830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7912
AlwNI CAG_nnn'CTG
Cuts at: 5655 7227 5655
Size: 1572 6340
ApaLI G'TGCA_C
Cuts at: 4346 4431 5969 7125 4346
Size: 85 1538 1156 5133
Fragments arranged by size:
5133 1538 1156 85
ApoI r'AATT_y
Cuts at: 1493 2378 2681 3092 3169 3290 3370 3677 4312
Size: 885 303 411 77 121 80 307 635
Cuts at: 4312 4938 5144 5221 5447 5599 7708 1493
Size: 626 206 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 635 626 411 307 303
226 206 152 121 80 77 77
AseI AT'TA_AT
Cuts at: 160 1032 2466 6466 160
Size: 872 1434 4000 1606
Fragments arranged by size:
4000 1606 1434 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7912
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 5237 1870
Size: 73 89 3205 4545
Fragments arranged by size:
4545 3205 89 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3919 3952 3919
Size: 33 7879
BamHI G'GATC_C
Cuts at: 3250 5242 5609 3250
Size: 1992 367 5553
Fragments arranged by size:
5553 1992 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2571 4397 5117 5233 6688
Size: 325 217 627 784 1826 720 116 1455
Cuts at: 6688 618
Size: 1842
Fragments arranged by size:
1842 1826 1455 784 720 627 325 217
116
BanII G_rGCy'C
Cuts at: 1391 3012 5231 1391
Size: 1621 2219 4072
Fragments arranged by size:
4072 2219 1621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 5006 928
Size: 971 213 2894 3834
Fragments arranged by size:
3834 2894 971 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6103 6137 1903
Size: 34 4166 34 3678
Fragments arranged by size:
4166 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5804 7014 5804
Size: 1210 6702
BclI T'GATC_A
Cuts at: 1548 3802 1548
Size: 2254 5658
BfrBI ATG'CAT
Cuts at: 4903 4903
Size: 7912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6523 136
Size: 107 122 71 631 605 4851 1525
Fragments arranged by size:
4851 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7907 7907
Size: 7912
BlpI GC'TnA_GC
Cuts at: 5329 5329
Size: 7912
Bme1580I G_kGCm'C
Cuts at: 1790 2574 3595 4350 4435 5973 7129 1790
Size: 784 1021 755 85 1538 1156 2573
Fragments arranged by size:
2573 1538 1156 1021 784 755 85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7912
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2882 4470 5643
Size: 416 53 83 399 24 1465 1588 1173
Cuts at: 5643 6601 442
Size: 958 1753
Fragments arranged by size:
1753 1588 1465 1173 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7912
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5031 5031
Size: 7912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 3359 4997 6572 1239
Size: 807 1313 1638 1575 2579
Fragments arranged by size:
2579 1638 1575 1313 807
Bpu10I CC'TnA_GC
Cuts at: 4613 4613
Size: 7912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5216 6037 6902 7200 5216
Size: 821 865 298 5928
Fragments arranged by size:
5928 865 821 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2022 4160 6575 882
Size: 617 523 2138 2415 2219
Fragments arranged by size:
2415 2219 2138 617 523
BsaAI yAC'GTr
Cuts at: 493 1204 2508 5209 5703 493
Size: 711 1304 2701 494 2702
Fragments arranged by size:
2702 2701 1304 711 494
BsaBI GATnn'nnATC
Cuts at: 3123 7906 3123
Size: 4783 3129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5719 6101 6736 275
Size: 53 83 186 936 4186 382 635 1451
Fragments arranged by size:
4186 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6345 6743 7017 7164 7575 6345
Size: 398 274 147 411 6682
Fragments arranged by size:
6682 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4364 4394 1178
Size: 30 165 30 2961 30 4696
Fragments arranged by size:
4696 2961 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2511 2834 4097 4382 4385 1186
Size: 1325 323 1263 285 3 4713
Fragments arranged by size:
4713 1325 1263 323 285 3
BseYI C'CCAG_C
Cuts at: 7115 7611 7115
Size: 496 7416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4387 1163
Size: 573 2651 4688
Fragments arranged by size:
4688 2651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5362 6123 7151 1540
Size: 275 186 3361 761 1028 2301
Fragments arranged by size:
3361 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3012 3940 4089 4350 4435 5973 6058 7129 3012
Size: 928 149 261 85 1538 85 1071 3795
Fragments arranged by size:
3795 1538 1071 928 261 149 85 85
BsmI GAATG_Cn'
Cuts at: 2327 3452 5423 5516 2327
Size: 1125 1971 93 4723
Fragments arranged by size:
4723 1971 1125 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2574 3012 3595 3940 4089 4350 4435
Size: 399 784 438 583 345 149 261 85
Cuts at: 4435 5231 5973 6058 7129 1391
Size: 796 742 85 1071 2174
Fragments arranged by size:
2174 1071 796 784 742 583 438 399
345 261 149 85 85
BspEI T'CCGG_A
Cuts at: 7575 7575
Size: 7912
BspHI T'CATG_A
Cuts at: 4417 5801 7531 4417
Size: 1384 1730 4798
Fragments arranged by size:
4798 1730 1384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4409 4674 5267 844
Size: 3565 265 593 3489
Fragments arranged by size:
3565 3489 593 265
BsrBI CCG'CTC
Cuts at: 1394 1823 4801 5799 1394
Size: 429 2978 998 3507
Fragments arranged by size:
3507 2978 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3345 3848 3982 4456 6407 6581 65
Size: 1183 2097 503 134 474 1951 174 1396
Fragments arranged by size:
2097 1951 1396 1183 503 474 174 134
BsrFI r'CCGG_y
Cuts at: 1700 1810 6556 6743 1700
Size: 110 4746 187 2869
Fragments arranged by size:
4746 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4512 96
Size: 4416 3496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7912
BssSI C'ACGA_G
Cuts at: 5972 6984 5972
Size: 1012 6900
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7912
BstBI TT'CG_AA
Cuts at: 2007 6716 2007
Size: 4709 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7912
BstYI r'GATC_y
Cuts at: 1972 3250 4493 5242 5609 5994 6011 7463 7549
Size: 1278 1243 749 367 385 17 1452 86
Cuts at: 7549 7561 7907 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1278 1243 749 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2073 2073
Size: 7912
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6403
Fragments arranged by size:
6403 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7173
Fragments arranged by size:
7173 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 2990 3012 3892 3940 5428 6222 6242 2179
Size: 811 22 880 48 1488 794 20 3849
Fragments arranged by size:
3849 1488 880 811 794 48 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7912
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7877
DraI TTT'AAA
Cuts at: 3375 3600 5216 3375
Size: 225 1616 6071
Fragments arranged by size:
6071 1616 225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6919 809
Size: 507 5603 1802
Fragments arranged by size:
5603 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4909 5359 6248
Size: 54 1425 50 275 18 3079 450 889
Cuts at: 6248 7877 8
Size: 1629 43
Fragments arranged by size:
3079 1629 1425 889 450 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5359 1537
Size: 275 3547 4090
Fragments arranged by size:
4090 3547 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2307 2488 3062 5842 1353
Size: 825 129 181 574 2780 3423
Fragments arranged by size:
3423 2780 825 574 181 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6499 6873 7019 387
Size: 1064 138 4910 374 146 1280
Fragments arranged by size:
4910 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3010 3010
Size: 7912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2632 3359 4997 5975 6572 7359
Size: 393 414 586 727 1638 978 597 787
Cuts at: 7359 7627 1239
Size: 268 1524
Fragments arranged by size:
1638 1524 978 787 727 597 586 414
393 268
EcoRI G'AATT_C
Cuts at: 3169 5221 5599 3169
Size: 2052 378 5482
Fragments arranged by size:
5482 2052 378
FspI TGC'GCA
Cuts at: 6418 6418
Size: 7912
HaeII r_GCGC'y
Cuts at: 1678 7059 1678
Size: 5381 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 3168 3200 4694
Size: 32 135 32 449 32 1115 32 1494
Cuts at: 4694 4726 6553 6585 6627 6659 1373
Size: 32 1827 32 42 32 2626
Fragments arranged by size:
2626 1827 1494 1115 449 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2088 3642 4182 4219 5256 5502
Size: 988 281 150 1554 540 37 1037 246
Cuts at: 5502 669
Size: 3079
Fragments arranged by size:
3079 1554 1037 988 540 281 246 150
37
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7912
HpaI GTT'AAC
Cuts at: 3642 4182 5502 3642
Size: 540 1320 6052
Fragments arranged by size:
6052 1320 540
KpnI G_GTAC'C
Cuts at: 5237 5237
Size: 7912
MfeI C'AATT_G
Cuts at: 4786 5511 4786
Size: 725 7187
MluI A'CGCG_T
Cuts at: 7788 7788
Size: 7912
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 2645 3258 3602 7026 7210 1305
Size: 714 626 613 344 3424 184 2007
Fragments arranged by size:
3424 2007 714 626 613 344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6090
Fragments arranged by size:
6090 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 2510 3340 3393 4002 4898 5870
Size: 648 1192 152 830 53 609 896 972
Cuts at: 5870 6229 6388 518
Size: 359 159 2042
Fragments arranged by size:
2042 1192 972 896 830 648 609 359
159 152 53
MspA1I CmG'CkG
Cuts at: 1606 2066 3933 5328 6005 7153 7398 1606
Size: 460 1867 1395 677 1148 245 2120
Fragments arranged by size:
2120 1867 1395 1148 677 460 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6403
Fragments arranged by size:
6403 957 552
NdeI CA'TA_TG
Cuts at: 387 2835 4895 387
Size: 2448 2060 3404
Fragments arranged by size:
3404 2448 2060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7912
NotI GC'GGCC_GC
Cuts at: 5359 5359
Size: 7912
NsiI A_TGCA'T
Cuts at: 4905 4905
Size: 7912
NspI r_CATG'y
Cuts at: 1132 1229 2357 2787 3491 3630 3671 3901 4907
Size: 97 1128 430 704 139 41 230 1006
Cuts at: 4907 5270 6815 1132
Size: 363 1545 2229
Fragments arranged by size:
2229 1545 1128 1006 704 430 363 230
139 97 41
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7912
PciI A'CATG_T
Cuts at: 3667 6811 3667
Size: 3144 4768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7912
PmeI GTTT'AAAC
Cuts at: 5216 5216
Size: 7912
PmlI CAC'GTG
Cuts at: 2508 2508
Size: 7912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4663 4695 5981 6013 7494 7526 4663
Size: 32 1286 32 1481 32 5049
Fragments arranged by size:
5049 1481 1286 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7912
PsiI TTA'TAA
Cuts at: 2676 3516 4818 5482 2676
Size: 840 1302 664 5106
Fragments arranged by size:
5106 1302 840 664
PstI C_TGCA'G
Cuts at: 830 3947 4289 5264 830
Size: 3117 342 975 3478
Fragments arranged by size:
3478 3117 975 342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7912
PvuII CAG'CTG
Cuts at: 2066 3933 2066
Size: 1867 6045
SacI G_AGCT'C
Cuts at: 3012 3012
Size: 7912
SalI G'TCGA_C
Cuts at: 1936 5254 1936
Size: 3318 4594
SapI GCTCTTCn'nnn_
Cuts at: 2178 3062 2178
Size: 884 7028
SbfI CC_TGCA'GG
Cuts at: 5264 5264
Size: 7912
ScaI AGT'ACT
Cuts at: 6160 7872 6160
Size: 1712 6200
SexAI A'CCwGG_T
Cuts at: 3434 3434
Size: 7912
SfcI C'TryA_G
Cuts at: 826 1045 3051 3057 3943 4285 4861 5260 6395
Size: 219 2006 6 886 342 576 399 1135
Cuts at: 6395 7076 7267 826
Size: 681 191 1471
Fragments arranged by size:
2006 1471 1135 886 681 576 399 342
219 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7912
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7912
SmaI CCC'GGG
Cuts at: 1872 2034 5239 1872
Size: 162 3205 4545
Fragments arranged by size:
4545 3205 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5195 6016 6917 7179 820
Size: 197 926 3252 821 901 262 1553
Fragments arranged by size:
3252 1553 926 901 821 262 197
SnaBI TAC'GTA
Cuts at: 493 5209 5703 493
Size: 4716 494 2702
Fragments arranged by size:
4716 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7912
SphI G_CATG'C
Cuts at: 1132 2357 4907 5270 1132
Size: 1225 2550 363 3774
Fragments arranged by size:
3774 2550 1225 363
SspI AAT'ATT
Cuts at: 5 52 2215 5836 5
Size: 47 2163 3621 2081
Fragments arranged by size:
3621 2163 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7912
StyI C'CwwG_G
Cuts at: 513 1065 2022 4747 5351 513
Size: 552 957 2725 604 3074
Fragments arranged by size:
3074 2725 957 604 552
SwaI ATTT'AAAT
Cuts at: 3600 3600
Size: 7912
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2500 2864 3167 5105 5939 5956 6109
Size: 1255 920 364 303 1938 834 17 153
Cuts at: 6109 6294 6739 325
Size: 185 445 1498
Fragments arranged by size:
1938 1498 1255 920 834 445 364 303
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4512 6158 7870
Size: 274 80 33 51 1443 2535 1646 1712
Cuts at: 7870 96
Size: 138
Fragments arranged by size:
2535 1712 1646 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7872 7904 7872
Size: 32 7880
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7912
XbaI T'CTAG_A
Cuts at: 4915 5248 4915
Size: 333 7579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4184 5161 4184
Size: 977 6935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7912
XmaI C'CCGG_G
Cuts at: 1870 2032 5237 1870
Size: 162 3205 4545
Fragments arranged by size:
4545 3205 162
XmnI GAAnn'nnTTC
Cuts at: 2382 2597 2852 4065 6041 2382
Size: 215 255 1213 1976 4253
Fragments arranged by size:
4253 1976 1213 255 215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5720 276
Size: 53 83 186 936 4186 2468
Fragments arranged by size:
4186 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI