Restriction map for
pFN21ASDA0558
(Circular) (Six-base) MAPSORT of: pFN21ASDA0558.seq Check: 1061 from: 1 to: 8164
pFN21ASDA0558 8164 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3457 3457
Size: 8164
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2135 3252 3327 5974
Size: 53 83 186 936 599 1117 75 2647
Cuts at: 5974 278
Size: 2468
Fragments arranged by size:
2647 2468 1117 936 599 186 83 75
53
AccI GT'mk_AC
Cuts at: 1937 2265 5507 1937
Size: 328 3242 4594
Fragments arranged by size:
4594 3242 328
Acc65I G'GTAC_C
Cuts at: 5485 5485
Size: 8164
AclI AA'CG_TT
Cuts at: 1563 3845 6291 6664 1563
Size: 2282 2446 373 3063
Fragments arranged by size:
3063 2446 2282 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2963 4062 4729 6227 7611 7879 1632
Size: 812 519 1099 667 1498 1384 268 1917
Fragments arranged by size:
1917 1498 1384 1099 812 667 519 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7967
AflIII A'CryG_T
Cuts at: 1203 3483 3693 4048 4254 7063 8040 1203
Size: 2280 210 355 206 2809 977 1327
Fragments arranged by size:
2809 2280 1327 977 355 210 206
AgeI A'CCGG_T
Cuts at: 6995 6995
Size: 8164
AhdI GACnn_n'nnGTC
Cuts at: 2983 6893 2983
Size: 3910 4254
AleI CACnn'nnGTG
Cuts at: 1166 2758 3902 4481 1166
Size: 1592 1144 579 4849
Fragments arranged by size:
4849 1592 1144 579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2708 2740 2708
Size: 32 8132
AlwNI CAG_nnn'CTG
Cuts at: 2422 3994 4219 4629 5140 5907 7479 2422
Size: 1572 225 410 511 767 1572 3107
Fragments arranged by size:
3107 1572 1572 767 511 410 225
ApaI G_GGCC'C
Cuts at: 2142 2245 2142
Size: 103 8061
ApaLI G'TGCA_C
Cuts at: 6221 7377 6221
Size: 1156 7008
ApoI r'AATT_y
Cuts at: 1493 5473 5699 5851 7960 1493
Size: 3980 226 152 2109 1697
Fragments arranged by size:
3980 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6718 160
Size: 872 5686 1606
Fragments arranged by size:
5686 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8164
AvaI C'yCGr_G
Cuts at: 1870 1943 3186 4302 4869 5018 5489 1870
Size: 73 1243 1116 567 149 471 4545
Fragments arranged by size:
4545 1243 1116 567 471 149 73
AvrII C'CTAG_G
Cuts at: 4495 4495
Size: 8164
BamHI G'GATC_C
Cuts at: 5494 5861 5494
Size: 367 7797
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2048 2117 2124 2195
Size: 325 217 627 242 19 69 7 71
Cuts at: 2195 3122 3904 5485 6940 618
Size: 927 782 1581 1455 1842
Fragments arranged by size:
1842 1581 1455 927 782 627 325 242
217 71 69 19 7
BanII G_rGCy'C
Cuts at: 1391 2142 2245 2697 2824 4134 4353 4491 5018
Size: 751 103 452 127 1310 219 138 527
Cuts at: 5018 5483 1391
Size: 465 4072
Fragments arranged by size:
4072 1310 751 527 465 452 219 138
127 103
BbeI G_GCGC'C
Cuts at: 2052 2128 2199 2052
Size: 76 71 8017
Fragments arranged by size:
8017 76 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 2761 2960 4300 4393 928
Size: 971 600 262 199 1340 93 4699
Fragments arranged by size:
4699 1340 971 600 262 199 93
BbvCI CC'TCA_GC
Cuts at: 4326 4839 4326
Size: 513 7651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6355 6389 1903
Size: 34 4418 34 3678
Fragments arranged by size:
4418 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6056 7266 6056
Size: 1210 6954
BclI T'GATC_A
Cuts at: 1548 3207 1548
Size: 1659 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5416 6775 136
Size: 107 122 71 631 605 3744 1359 1525
Fragments arranged by size:
3744 1525 1359 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8159 8159
Size: 8164
BlpI GC'TnA_GC
Cuts at: 2526 4490 5581 2526
Size: 1964 1091 5109
Fragments arranged by size:
5109 1964 1091
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2245 3125 4837 4887 6225 7381 1790
Size: 352 103 880 1712 50 1338 1156 2573
Fragments arranged by size:
2573 1712 1338 1156 880 352 103 50
BmgBI CAC'GTC
Cuts at: 1318 3607 1318
Size: 2289 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 3509 3515
Size: 416 53 83 399 24 831 1261 6
Cuts at: 3515 5050 5895 6853 442
Size: 1535 845 958 1753
Fragments arranged by size:
1753 1535 1261 958 845 831 416 399
83 53 24 6
BmtI G_CTAG'C
Cuts at: 1056 4577 1056
Size: 3521 4643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2735 4134 4460 4466 5101 6824 1239
Size: 1034 462 1399 326 6 635 1723 2579
Fragments arranged by size:
2579 1723 1399 1034 635 462 326 6
Bpu10I CC'TnA_GC
Cuts at: 4326 4347 4839 4326
Size: 21 492 7651
Fragments arranged by size:
7651 492 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3698 5412 6289 7154 7452 3698
Size: 1714 877 865 298 4410
Fragments arranged by size:
4410 1714 877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2851 5263 5383 6827 882
Size: 617 1352 2412 120 1444 2219
Fragments arranged by size:
2412 2219 1444 1352 617 120
BsaAI yAC'GTr
Cuts at: 493 1204 3694 4257 5461 5955 493
Size: 711 2490 563 1204 494 2702
Fragments arranged by size:
2702 2490 1204 711 563 494
BsaBI GATnn'nnATC
Cuts at: 8158 8158
Size: 8164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2125 2132 2196
Size: 53 83 186 936 516 76 7 64
Cuts at: 2196 2248 2292 2511 3249 3324 4530 5370 5971
Size: 52 44 219 738 75 1206 840 601
Cuts at: 5971 6353 6988 275
Size: 382 635 1451
Fragments arranged by size:
1451 1206 936 840 738 635 601 516
382 219 186 83 76 75 64 53
52 44 7
BsaWI w'CCGG_w
Cuts at: 2325 3276 5280 6597 6995 7269 7416 7827 2325
Size: 951 2004 1317 398 274 147 411 2662
Fragments arranged by size:
2662 2004 1317 951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2657 2687 2708 2738 4772
Size: 30 165 30 1254 30 21 30 2034
Cuts at: 4772 4802 5379 5409 1178
Size: 30 577 30 3933
Fragments arranged by size:
3933 2034 1254 577 165 30 30 30
30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5387 1186
Size: 4201 3963
BseYI C'CCAG_C
Cuts at: 2065 2217 4025 5233 5365 7367 7863 2065
Size: 152 1808 1208 132 2002 496 2366
Fragments arranged by size:
2366 2002 1808 1208 496 152 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 3080 3122 3263 3491 3686 4166
Size: 573 699 645 42 141 228 195 480
Cuts at: 4166 4848 5206 5285 1163
Size: 682 358 79 4042
Fragments arranged by size:
4042 699 682 645 573 480 358 228
195 141 79 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5420 5614 6375 7403 1540
Size: 275 186 3419 194 761 1028 2301
Fragments arranged by size:
3419 2301 1028 761 275 194 186
BsiHKAI G_wGCw'C
Cuts at: 2697 3920 3945 4134 4491 6225 6310 7381 2697
Size: 1223 25 189 357 1734 85 1071 3480
Fragments arranged by size:
3480 1734 1223 1071 357 189 85 25
BsmI GAATG_Cn'
Cuts at: 4093 5675 5768 4093
Size: 1582 93 6489
Fragments arranged by size:
6489 1582 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5015 5216 5015
Size: 201 7963
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2245 2697 2824 3125 3920 3945
Size: 399 352 103 452 127 301 795 25
Cuts at: 3945 4134 4353 4491 4837 4887 5018 5483 6225
Size: 189 219 138 346 50 131 465 742
Cuts at: 6225 6310 7381 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 795 742 465 452 399 352
346 301 219 189 138 131 127 103
85 50 25
BspEI T'CCGG_A
Cuts at: 7827 7827
Size: 8164
BspHI T'CATG_A
Cuts at: 3210 5043 6053 7783 3210
Size: 1833 1010 1730 3591
Fragments arranged by size:
3591 1833 1730 1010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2051 2755 3457 3988 4728 5519 844
Size: 1207 704 702 531 740 791 3489
Fragments arranged by size:
3489 1207 791 740 704 702 531
BsrBI CCG'CTC
Cuts at: 1394 1823 2931 6051 1394
Size: 429 1108 3120 3507
Fragments arranged by size:
3507 3120 1108 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4056 6659 6833 65
Size: 1183 2808 2603 174 1396
Fragments arranged by size:
2808 2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 3140 5205 6808 6995 1700
Size: 110 222 1108 2065 1603 187 2869
Fragments arranged by size:
2869 2065 1603 1108 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 2644 96
Size: 2548 5616
BssHII G'CGCG_C
Cuts at: 1925 2078 1925
Size: 153 8011
BssSI C'ACGA_G
Cuts at: 6224 7236 6224
Size: 1012 7152
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2230 4832 5193 5392 794
Size: 1436 2602 361 199 3566
Fragments arranged by size:
3566 2602 1436 361 199
BstBI TT'CG_AA
Cuts at: 2007 6968 2007
Size: 4961 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8164
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 3445 2896
Size: 549 7615
BstYI r'GATC_y
Cuts at: 1972 2649 3375 3795 3990 4246 5494 5861 6246
Size: 677 726 420 195 256 1248 367 385
Cuts at: 6246 6263 7715 7801 7813 8159 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1248 726 677 420 385 367
346 256 195 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2582 2582
Size: 8164
BtgI C'CryG_G
Cuts at: 513 1065 2022 2813 3303 3438 4390 4998 5341
Size: 552 957 791 490 135 952 608 343
Cuts at: 5341 513
Size: 3336
Fragments arranged by size:
3336 957 952 791 608 552 490 343
135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7425
Fragments arranged by size:
7425 683 56
BtsI GCAGTG_nn'
Cuts at: 2753 2929 3344 3479 4169 4527 5227 5258 5680
Size: 176 415 135 690 358 700 31 422
Cuts at: 5680 6474 6494 2753
Size: 794 20 4423
Fragments arranged by size:
4423 794 700 690 422 415 358 176
135 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8129
DraI TTT'AAA
Cuts at: 5468 5468
Size: 8164
DraIII CAC_nnn'GTG
Cuts at: 2814 3058 2814
Size: 244 7920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7171 809
Size: 507 5855 1802
Fragments arranged by size:
5855 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2805 3114 3269
Size: 54 1425 50 275 18 975 309 155
Cuts at: 3269 4269 5169 5203 5338 5417 5611 6500 8129
Size: 1000 900 34 135 79 194 889 1629
Cuts at: 8129 8
Size: 43
Fragments arranged by size:
1629 1425 1000 975 900 889 309 275
194 155 135 79 54 50 43 34
18
EagI C'GGCC_G
Cuts at: 1537 1812 5417 5611 1537
Size: 275 3605 194 4090
Fragments arranged by size:
4090 3605 275 194
EarI CTCTTCn'nnn_
Cuts at: 1353 2420 3786 6094 1353
Size: 1067 1366 2308 3423
Fragments arranged by size:
3423 2308 1366 1067
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6751 7125 7271 387
Size: 1064 138 5162 374 146 1280
Fragments arranged by size:
5162 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 4132 4489 2695
Size: 1437 357 6370
Fragments arranged by size:
6370 1437 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2444 2735 2963 4062 4134 4460
Size: 393 641 171 291 228 1099 72 326
Cuts at: 4460 4466 4729 5101 6227 6824 7611 7879 1239
Size: 6 263 372 1126 597 787 268 1524
Fragments arranged by size:
1524 1126 1099 787 641 597 393 372
326 291 268 263 228 171 72 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 3059 3548 2336
Size: 723 489 6952
Fragments arranged by size:
6952 723 489
EcoO109I rG'GnC_Cy
Cuts at: 2139 2419 2602 2710 2877 3525 4081 5346 2139
Size: 280 183 108 167 648 556 1265 4957
Fragments arranged by size:
4957 1265 648 556 280 183 167 108
EcoRI G'AATT_C
Cuts at: 5473 5851 5473
Size: 378 7786
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 8164
FspI TGC'GCA
Cuts at: 3074 6670 3074
Size: 3596 4568
HaeII r_GCGC'y
Cuts at: 1678 2052 2128 2199 2781 7311 1678
Size: 374 76 71 582 4530 2531
Fragments arranged by size:
4530 2531 582 374 76 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2969 3001 6805 6837 6879
Size: 32 135 32 1397 32 3804 32 42
Cuts at: 6879 6911 1373
Size: 32 2626
Fragments arranged by size:
3804 2626 1397 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 2626 4651 5508 5754 669
Size: 988 281 328 360 2025 857 246 3079
Fragments arranged by size:
3079 2025 988 857 360 328 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8164
HpaI GTT'AAC
Cuts at: 4651 5754 4651
Size: 1103 7061
KasI G'GCGC_C
Cuts at: 2048 2124 2195 2048
Size: 76 71 8017
Fragments arranged by size:
8017 76 71
KpnI G_GTAC'C
Cuts at: 5489 5489
Size: 8164
MfeI C'AATT_G
Cuts at: 3265 5763 3265
Size: 2498 5666
MluI A'CGCG_T
Cuts at: 8040 8040
Size: 8164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3201 3782 7278 7462 1305
Size: 1896 581 3496 184 2007
Fragments arranged by size:
3496 2007 1896 581 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3116 3271 10
Size: 54 1425 343 1284 155 4903
Fragments arranged by size:
4903 1425 1284 343 155 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2758 2906 3318 3605 3902 4395
Size: 648 861 731 148 412 287 297 493
Cuts at: 4395 4419 4481 6122 6481 6640 518
Size: 24 62 1641 359 159 2042
Fragments arranged by size:
2042 1641 861 731 648 493 412 359
297 287 159 148 62 24
MspA1I CmG'CkG
Cuts at: 1606 2152 2179 2469 3166 3334 3580 4219 4325
Size: 546 27 290 697 168 246 639 106
Cuts at: 4325 4438 4481 4980 5000 5037 5182 5237 5343
Size: 113 43 499 20 37 145 55 106
Cuts at: 5343 5580 6257 7405 7650 1606
Size: 237 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 639 546 499 290
246 245 237 168 145 113 106 106
55 43 37 27 20
NaeI GCC'GGC
Cuts at: 2034 3142 5207 2034
Size: 1108 2065 4991
Fragments arranged by size:
4991 2065 1108
NarI GG'CG_CC
Cuts at: 2049 2125 2196 2049
Size: 76 71 8017
Fragments arranged by size:
8017 76 71
NcoI C'CATG_G
Cuts at: 513 1065 2022 3438 513
Size: 552 957 1416 5239
Fragments arranged by size:
5239 1416 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8164
NgoMIV G'CCGG_C
Cuts at: 2032 3140 5205 2032
Size: 1108 2065 4991
Fragments arranged by size:
4991 2065 1108
NheI G'CTAG_C
Cuts at: 1052 4573 1052
Size: 3521 4643
NotI GC'GGCC_GC
Cuts at: 5611 5611
Size: 8164
NspI r_CATG'y
Cuts at: 1132 1229 5522 7067 1132
Size: 97 4293 1545 2229
Fragments arranged by size:
4293 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3560 1348
Size: 2212 5952
PciI A'CATG_T
Cuts at: 7063 7063
Size: 8164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2814 1108
Size: 1706 6458
PfoI T'CCnGG_A
Cuts at: 3735 3759 3735
Size: 24 8140
PmeI GTTT'AAAC
Cuts at: 5468 5468
Size: 8164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 6233 6265 7746 7778 2708
Size: 32 3493 32 1481 32 3094
Fragments arranged by size:
3493 3094 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 3525 4081 2602
Size: 923 556 6685
Fragments arranged by size:
6685 923 556
PshAI GACnn'nnGTC
Cuts at: 1613 2960 1613
Size: 1347 6817
PsiI TTA'TAA
Cuts at: 5734 5734
Size: 8164
PspOMI G'GGCC_C
Cuts at: 2138 2241 2138
Size: 103 8061
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 8132
PstI C_TGCA'G
Cuts at: 830 2446 3171 3339 3999 5217 5516 830
Size: 1616 725 168 660 1218 299 3478
Fragments arranged by size:
3478 1616 1218 725 660 299 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8164
PvuII CAG'CTG
Cuts at: 3166 3580 4219 4325 4481 4980 5037 3166
Size: 414 639 106 156 499 57 6293
Fragments arranged by size:
6293 639 499 414 156 106 57
SacI G_AGCT'C
Cuts at: 2697 4134 4491 2697
Size: 1437 357 6370
Fragments arranged by size:
6370 1437 357
SacII CC_GC'GG
Cuts at: 5001 5344 5001
Size: 343 7821
SalI G'TCGA_C
Cuts at: 1936 2264 5506 1936
Size: 328 3242 4594
Fragments arranged by size:
4594 3242 328
SanDI GG'GwC_CC
Cuts at: 3525 3525
Size: 8164
SbfI CC_TGCA'GG
Cuts at: 3999 5516 3999
Size: 1517 6647
ScaI AGT'ACT
Cuts at: 4073 5060 6412 8124 4073
Size: 987 1352 1712 4113
Fragments arranged by size:
4113 1712 1352 987
SexAI A'CCwGG_T
Cuts at: 3054 4728 3054
Size: 1674 6490
SfcI C'TryA_G
Cuts at: 826 1045 2206 2442 3167 3335 3925 3952 3995
Size: 219 1161 236 725 168 590 27 43
Cuts at: 3995 5213 5512 6647 7328 7519 826
Size: 1218 299 1135 681 191 1471
Fragments arranged by size:
1471 1218 1161 1135 725 681 590 299
236 219 191 168 43 27
SfoI GGC'GCC
Cuts at: 2050 2126 2197 2050
Size: 76 71 8017
Fragments arranged by size:
8017 76 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8164
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8164
SmaI CCC'GGG
Cuts at: 1872 4871 5491 1872
Size: 2999 620 4545
Fragments arranged by size:
4545 2999 620
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3713 5427 6268 7169 7431 820
Size: 197 926 1770 1714 841 901 262 1553
Fragments arranged by size:
1770 1714 1553 926 901 841 262 197
SnaBI TAC'GTA
Cuts at: 493 5461 5955 493
Size: 4968 494 2702
Fragments arranged by size:
4968 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8164
SphI G_CATG'C
Cuts at: 1132 5522 1132
Size: 4390 3774
SspI AAT'ATT
Cuts at: 5 52 6088 5
Size: 47 6036 2081
Fragments arranged by size:
6036 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8164
StyI C'CwwG_G
Cuts at: 513 1065 2022 2514 3117 3438 4495 5603 513
Size: 552 957 492 603 321 1057 1108 3074
Fragments arranged by size:
3074 1108 1057 957 603 552 492 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2902 3892 4847 6191 6208 6361 6546
Size: 1255 1322 990 955 1344 17 153 185
Cuts at: 6546 6991 325
Size: 445 1498
Fragments arranged by size:
1498 1344 1322 1255 990 955 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2644 3261
Size: 274 80 33 51 1443 525 142 617
Cuts at: 3261 4071 5058 6410 8122 96
Size: 810 987 1352 1712 138
Fragments arranged by size:
1712 1443 1352 987 810 617 525 274
142 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3334 3366 4683 4715 8124 8156 3334
Size: 32 1317 32 3409 32 3342
Fragments arranged by size:
3409 3342 1317 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2897 1316
Size: 1581 6583
XbaI T'CTAG_A
Cuts at: 3418 5500 3418
Size: 2082 6082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4768 5198 4768
Size: 430 7734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8164
XmaI C'CCGG_G
Cuts at: 1870 4869 5489 1870
Size: 2999 620 4545
Fragments arranged by size:
4545 2999 620
XmnI GAAnn'nnTTC
Cuts at: 2333 2723 3086 6293 2333
Size: 390 363 3207 4204
Fragments arranged by size:
4204 3207 390 363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2133 3250 3325 5972
Size: 53 83 186 936 599 1117 75 2647
Cuts at: 5972 276
Size: 2468
Fragments arranged by size:
2647 2468 1117 936 599 186 83 75
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BplI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI RsrII
SapI SfiI SrfI SwaI