Restriction map for
pFN21ASDA0552
(Circular) (Six-base) MAPSORT of: pFN21ASDA0552.seq Check: 7756 from: 1 to: 6748
pFN21ASDA0552 6748 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3142 3187 3635 3142
Size: 45 448 6255
Fragments arranged by size:
6255 448 45
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4558 278
Size: 53 83 186 936 3022 2468
Fragments arranged by size:
3022 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4091 1937
Size: 2154 4594
Acc65I G'GTAC_C
Cuts at: 2189 4069 2189
Size: 1880 4868
AclI AA'CG_TT
Cuts at: 1563 4875 5248 1563
Size: 3312 373 3063
Fragments arranged by size:
3312 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 3410 3499 4811 6195 6463 1632
Size: 1766 12 89 1312 1384 268 1917
Fragments arranged by size:
1917 1766 1384 1312 268 89 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6551
AflIII A'CryG_T
Cuts at: 1203 5647 6624 1203
Size: 4444 977 1327
Fragments arranged by size:
4444 1327 977
AgeI A'CCGG_T
Cuts at: 5579 5579
Size: 6748
AhdI GACnn_n'nnGTC
Cuts at: 5477 5477
Size: 6748
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6748
AlwNI CAG_nnn'CTG
Cuts at: 2734 2839 2992 3079 3388 3919 4491 6063 2734
Size: 105 153 87 309 531 572 1572 3419
Fragments arranged by size:
3419 1572 572 531 309 153 105 87
ApaI G_GGCC'C
Cuts at: 2600 3739 3793 2600
Size: 1139 54 5555
Fragments arranged by size:
5555 1139 54
ApaLI G'TGCA_C
Cuts at: 2976 4805 5961 2976
Size: 1829 1156 3763
Fragments arranged by size:
3763 1829 1156
ApoI r'AATT_y
Cuts at: 1493 2533 2551 4057 4283 4435 6544 1493
Size: 1040 18 1506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1506 1040 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 5302 160
Size: 872 4270 1606
Fragments arranged by size:
4270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 3792 4016 4073 1870
Size: 73 292 1557 224 57 4545
Fragments arranged by size:
4545 1557 292 224 73 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2408 2441 2408
Size: 33 6715
BamHI G'GATC_C
Cuts at: 4078 4445 4078
Size: 367 6381
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 2737 3806 4069 5524
Size: 325 217 627 402 548 1069 263 1455
Cuts at: 5524 618
Size: 1842
Fragments arranged by size:
1842 1455 1069 627 548 402 325 263
217
BanII G_rGCy'C
Cuts at: 1391 2436 2600 2918 3739 3793 4067 1391
Size: 1045 164 318 821 54 274 4072
Fragments arranged by size:
4072 1045 821 318 274 164 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3542 3576 4939 4973 1903
Size: 34 1605 34 1363 34 3678
Fragments arranged by size:
3678 1605 1363 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4640 5850 4640
Size: 1210 5538
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 2604 2613
Size: 107 122 71 631 605 566 366 9
Cuts at: 2613 2913 3668 3773 5359 136
Size: 300 755 105 1586 1525
Fragments arranged by size:
1586 1525 755 631 605 566 366 300
122 107 105 71 9
BglII A'GATC_T
Cuts at: 3366 6743 3366
Size: 3377 3371
BlpI GC'TnA_GC
Cuts at: 3497 3780 3884 4165 3497
Size: 283 104 281 6080
Fragments arranged by size:
6080 283 281 104
Bme1580I G_kGCm'C
Cuts at: 1790 2561 2600 2980 3169 3739 3793 4809 5965
Size: 771 39 380 189 570 54 1016 1156
Cuts at: 5965 1790
Size: 2573
Fragments arranged by size:
2573 1156 1016 771 570 380 189 54
39
BmgBI CAC'GTC
Cuts at: 1318 3164 1318
Size: 1846 4902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 4479 5437
Size: 416 53 83 399 24 1550 1512 958
Cuts at: 5437 442
Size: 1753
Fragments arranged by size:
1753 1550 1512 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2628 2701 2851 3014 3068 3101 3293
Size: 815 574 73 150 163 54 33 192
Cuts at: 3293 3557 3860 3941 5408 1239
Size: 264 303 81 1467 2579
Fragments arranged by size:
2579 1467 815 574 303 264 192 163
150 81 73 54 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3557 4873 5738 6036 3557
Size: 1316 865 298 4269
Fragments arranged by size:
4269 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 5411 882
Size: 617 804 3108 2219
Fragments arranged by size:
3108 2219 804 617
BsaAI yAC'GTr
Cuts at: 493 1204 3892 4045 4539 493
Size: 711 2688 153 494 2702
Fragments arranged by size:
2702 2688 711 494 153
BsaBI GATnn'nnATC
Cuts at: 6742 6742
Size: 6748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4555 4937 5572 275
Size: 53 83 186 936 3022 382 635 1451
Fragments arranged by size:
3022 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5181 5579 5853 6000 6411 5181
Size: 398 274 147 411 5518
Fragments arranged by size:
5518 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2436 2466 2626 2647 2656
Size: 30 165 30 1033 30 160 21 9
Cuts at: 2656 2677 2990 3020 3955 3985 1178
Size: 21 313 30 935 30 3941
Fragments arranged by size:
3941 1033 935 313 165 160 30 30
30 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3011 3098 3251 3500 3836 3857 4001 1186
Size: 1825 87 153 249 336 21 144 3933
Fragments arranged by size:
3933 1825 336 249 153 144 87 21
BseYI C'CCAG_C
Cuts at: 2435 3168 3177 3541 3975 5951 6447 2435
Size: 733 9 364 434 1976 496 2736
Fragments arranged by size:
2736 1976 733 496 434 364 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6175
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2504 3603 4198 4959 5987 1540
Size: 275 186 503 1099 595 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 595 503 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 2980 4809 4894 5965 2486
Size: 494 1829 85 1071 3269
Fragments arranged by size:
3269 1829 1071 494 85
BsmI GAATG_Cn'
Cuts at: 4259 4352 4259
Size: 93 6655
BsmBI CGTCTCn'nnnn_
Cuts at: 2030 2030
Size: 6748
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2436 2486 2561 2600 2918 2980 3169
Size: 399 646 50 75 39 318 62 189
Cuts at: 3169 3739 3793 4067 4809 4894 5965 1391
Size: 570 54 274 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 646 570 399 318 274
189 85 75 62 54 50 39
BspEI T'CCGG_A
Cuts at: 6411 6411
Size: 6748
BspHI T'CATG_A
Cuts at: 4637 6367 4637
Size: 1730 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3142 3187 3635 4103 844
Size: 2298 45 448 468 3489
Fragments arranged by size:
3489 2298 468 448 45
BsrBI CCG'CTC
Cuts at: 1394 1823 3001 3088 3106 3726 3784 4635 1394
Size: 429 1178 87 18 620 58 851 3507
Fragments arranged by size:
3507 1178 851 620 429 87 58 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 5243 5417 65
Size: 1183 3995 174 1396
Fragments arranged by size:
3995 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3577 5392 5579 1700
Size: 110 1767 1815 187 2869
Fragments arranged by size:
2869 1815 1767 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6748
BssHII G'CGCG_C
Cuts at: 1925 2050 3172 1925
Size: 125 1122 5501
Fragments arranged by size:
5501 1122 125
BssSI C'ACGA_G
Cuts at: 4808 5820 4808
Size: 1012 5736
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3202 3956 794
Size: 2408 754 3586
Fragments arranged by size:
3586 2408 754
BstBI TT'CG_AA
Cuts at: 2007 5552 2007
Size: 3545 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6748
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 3359 2455
Size: 904 5844
BstYI r'GATC_y
Cuts at: 1972 3366 4078 4445 4830 4847 6299 6385 6397
Size: 1394 712 367 385 17 1452 86 12
Cuts at: 6397 6743 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1394 712 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2583 2583
Size: 6748
BtgI C'CryG_G
Cuts at: 513 1065 2022 2090 2130 3591 3693 513
Size: 552 957 68 40 1461 102 3568
Fragments arranged by size:
3568 1461 957 552 102 68 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6009
Fragments arranged by size:
6009 683 56
BtsI GCAGTG_nn'
Cuts at: 2152 2248 2254 2344 2401 2410 2778 4264 5058
Size: 96 6 90 57 9 368 1486 794
Cuts at: 5058 5078 2152
Size: 20 3822
Fragments arranged by size:
3822 1486 794 368 96 90 57 20
9 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6713
DraI TTT'AAA
Cuts at: 4052 4052
Size: 6748
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5755 809
Size: 507 4439 1802
Fragments arranged by size:
4439 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2457 2501
Size: 54 1425 50 275 18 297 330 44
Cuts at: 2501 2900 3114 3285 3600 3621 3669 4195 5084
Size: 399 214 171 315 21 48 526 889
Cuts at: 5084 6713 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 526 399 330 315 297
275 214 171 54 50 48 44 43
21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2501 3600 4195 1537
Size: 275 689 1099 595 4090
Fragments arranged by size:
4090 1099 689 595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 3261 4678 1353
Size: 821 1087 1417 3423
Fragments arranged by size:
3423 1417 1087 821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2631 2943 3419 3722 5335 5709
Size: 1064 138 1042 312 476 303 1613 374
Cuts at: 5709 5855 387
Size: 146 1280
Fragments arranged by size:
1613 1280 1064 1042 476 374 312 303
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2628 2701 2851 3014 3068 3101
Size: 393 422 574 73 150 163 54 33
Cuts at: 3101 3293 3398 3410 3499 3557 3860 3941 4811
Size: 192 105 12 89 58 303 81 870
Cuts at: 4811 5408 6195 6463 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 870 787 597 574 422 393 303
268 192 163 150 105 89 81 73
58 54 33 12
EcoO109I rG'GnC_Cy
Cuts at: 2068 2596 2597 2836 2844 3184 3736 2068
Size: 528 1 239 8 340 552 5080
Fragments arranged by size:
5080 552 528 340 239 8 1
EcoRI G'AATT_C
Cuts at: 4057 4435 4057
Size: 378 6370
FspI TGC'GCA
Cuts at: 5254 5254
Size: 6748
HaeII r_GCGC'y
Cuts at: 1678 3584 3802 3829 5895 1678
Size: 1906 218 27 2066 2531
Fragments arranged by size:
2531 2066 1906 218 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2626 2658 3640
Size: 32 135 32 896 32 126 32 982
Cuts at: 3640 3672 5389 5421 5463 5495 1373
Size: 32 1717 32 42 32 2626
Fragments arranged by size:
2626 1717 982 896 135 126 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4092 4338 669
Size: 988 281 2154 246 3079
Fragments arranged by size:
3079 2154 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6748
HpaI GTT'AAC
Cuts at: 4338 4338
Size: 6748
KpnI G_GTAC'C
Cuts at: 2193 4073 2193
Size: 1880 4868
MfeI C'AATT_G
Cuts at: 4347 4347
Size: 6748
MluI A'CGCG_T
Cuts at: 6624 6624
Size: 6748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5862 6046 1305
Size: 4557 184 2007
Fragments arranged by size:
4557 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 2459 3623 10
Size: 54 1425 343 297 330 1164 3135
Fragments arranged by size:
3135 1425 1164 343 330 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2349 4706 5065 5224 518
Size: 648 1183 2357 359 159 2042
Fragments arranged by size:
2357 2042 1183 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2379 2648 2814 3138 3193 3229 3262 3388
Size: 773 269 166 324 55 36 33 126
Cuts at: 3388 3451 3493 3674 3877 3883 3919 4164 4841
Size: 63 42 181 203 6 36 245 677
Cuts at: 4841 5989 6234 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 773 677 324 269 245 245
203 181 166 126 63 55 42 36
36 33 6
NaeI GCC'GGC
Cuts at: 3579 3579
Size: 6748
NcoI C'CATG_G
Cuts at: 513 1065 2022 2130 513
Size: 552 957 108 5131
Fragments arranged by size:
5131 957 552 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6748
NgoMIV G'CCGG_C
Cuts at: 3577 3577
Size: 6748
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6748
NotI GC'GGCC_GC
Cuts at: 4195 4195
Size: 6748
NspI r_CATG'y
Cuts at: 1132 1229 4106 5651 1132
Size: 97 2877 1545 2229
Fragments arranged by size:
2877 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2060 1348
Size: 712 6036
PciI A'CATG_T
Cuts at: 5647 5647
Size: 6748
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2651 2734 2839 2992 3079 3919 1108
Size: 1543 83 105 153 87 840 3937
Fragments arranged by size:
3937 1543 840 153 105 87 83
PfoI T'CCnGG_A
Cuts at: 2985 2985
Size: 6748
PmeI GTTT'AAAC
Cuts at: 4052 4052
Size: 6748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4817 4849 6330 6362 4817
Size: 32 1481 32 5203
Fragments arranged by size:
5203 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2836 2844 2068
Size: 768 8 5972
Fragments arranged by size:
5972 768 8
PshAI GACnn'nnGTC
Cuts at: 1613 2295 1613
Size: 682 6066
PsiI TTA'TAA
Cuts at: 4318 4318
Size: 6748
PspOMI G'GGCC_C
Cuts at: 2596 3735 3789 2596
Size: 1139 54 5555
Fragments arranged by size:
5555 1139 54
PspXI vC'TCGA_Gb
Cuts at: 4016 4016
Size: 6748
PstI C_TGCA'G
Cuts at: 830 3137 3198 3213 3234 3765 3882 4100 830
Size: 2307 61 15 21 531 117 218 3478
Fragments arranged by size:
3478 2307 531 218 117 61 21 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6748
PvuII CAG'CTG
Cuts at: 3193 3229 3388 3451 3493 3877 3883 3919 3193
Size: 36 159 63 42 384 6 36 6022
Fragments arranged by size:
6022 384 159 63 42 36 36 6
SalI G'TCGA_C
Cuts at: 1936 4090 1936
Size: 2154 4594
SanDI GG'GwC_CC
Cuts at: 2068 2068
Size: 6748
SapI GCTCTTCn'nnn_
Cuts at: 2174 3261 2174
Size: 1087 5661
SbfI CC_TGCA'GG
Cuts at: 4100 4100
Size: 6748
ScaI AGT'ACT
Cuts at: 4996 6708 4996
Size: 1712 5036
SfcI C'TryA_G
Cuts at: 826 1045 2710 2722 3133 3146 3188 3194 3209
Size: 219 1665 12 411 13 42 6 15
Cuts at: 3209 3230 3761 3878 4096 5231 5912 6103 826
Size: 21 531 117 218 1135 681 191 1471
Fragments arranged by size:
1665 1471 1135 681 531 411 219 218
191 117 42 21 15 13 12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2604 3668 2604
Size: 1064 5684
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6748
SmaI CCC'GGG
Cuts at: 1872 3794 4075 1872
Size: 1922 281 4545
Fragments arranged by size:
4545 1922 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3572 4016 4852 5753 6015 820
Size: 197 926 1629 444 836 901 262 1553
Fragments arranged by size:
1629 1553 926 901 836 444 262 197
SnaBI TAC'GTA
Cuts at: 493 4045 4539 493
Size: 3552 494 2702
Fragments arranged by size:
3552 2702 494
SpeI A'CTAG_T
Cuts at: 152 2494 152
Size: 2342 4406
SphI G_CATG'C
Cuts at: 1132 4106 1132
Size: 2974 3774
SspI AAT'ATT
Cuts at: 5 52 4672 5
Size: 47 4620 2081
Fragments arranged by size:
4620 2081 47
StuI AGG'CCT
Cuts at: 1517 2940 3998 1517
Size: 1423 1058 4267
Fragments arranged by size:
4267 1423 1058
StyI C'CwwG_G
Cuts at: 513 1065 2022 2130 3684 4187 513
Size: 552 957 108 1554 503 3074
Fragments arranged by size:
3074 1554 957 552 503 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2457 2461 4775 4792 4945 5130 5575
Size: 1255 877 4 2314 17 153 185 445
Cuts at: 5575 325
Size: 1498
Fragments arranged by size:
2314 1498 1255 877 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4994 6706 96
Size: 274 80 33 51 1443 3017 1712 138
Fragments arranged by size:
3017 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 2647 2679 2990 3022 3323 3355 3955
Size: 32 179 32 311 32 301 32 600
Cuts at: 3955 3987 6708 6740 2436
Size: 32 2721 32 2444
Fragments arranged by size:
2721 2444 600 311 301 179 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6748
XbaI T'CTAG_A
Cuts at: 4084 4084
Size: 6748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2137 2182 2915 3538 2029
Size: 108 45 733 623 5239
Fragments arranged by size:
5239 733 623 108 45
XhoI C'TCGA_G
Cuts at: 1943 4016 1943
Size: 2073 4675
XmaI C'CCGG_G
Cuts at: 1870 3792 4073 1870
Size: 1922 281 4545
Fragments arranged by size:
4545 1922 281
XmnI GAAnn'nnTTC
Cuts at: 4877 4877
Size: 6748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4556 276
Size: 53 83 186 936 3022 2468
Fragments arranged by size:
3022 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PsrI RsrII SacI SacII SexAI SfoI SrfI SwaI