Restriction map for
pFN21ASDA0550
(Circular) (Six-base) MAPSORT of: pFN21ASDA0550.seq Check: 4637 from: 1 to: 9295
pFN21ASDA0550 9295 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2621 2947 3785 4039 2621
Size: 326 838 254 7877
Fragments arranged by size:
7877 838 326 254
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7105 278
Size: 53 83 186 936 5569 2468
Fragments arranged by size:
5569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2600 5598 6638 1937
Size: 663 2998 1040 4594
Fragments arranged by size:
4594 2998 1040 663
Acc65I G'GTAC_C
Cuts at: 6616 6616
Size: 9295
AclI AA'CG_TT
Cuts at: 1563 7422 7795 1563
Size: 5859 373 3063
Fragments arranged by size:
5859 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4638 5739 7358 8742 9010 1632
Size: 3006 1101 1619 1384 268 1917
Fragments arranged by size:
3006 1917 1619 1384 1101 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9098
AflIII A'CryG_T
Cuts at: 1203 2927 3101 8194 9171 1203
Size: 1724 174 5093 977 1327
Fragments arranged by size:
5093 1724 1327 977 174
AgeI A'CCGG_T
Cuts at: 8126 8126
Size: 9295
AhdI GACnn_n'nnGTC
Cuts at: 2650 8024 2650
Size: 5374 3921
AleI CACnn'nnGTG
Cuts at: 1166 4265 6170 1166
Size: 3099 1905 4291
Fragments arranged by size:
4291 3099 1905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3321 5515 1184
Size: 2137 2194 4964
Fragments arranged by size:
4964 2194 2137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2914 2946 3076 3108 3406 3438 2914
Size: 32 130 32 298 32 8771
Fragments arranged by size:
8771 298 130 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2656 2722 3666 4160 5114 7038 8610 2656
Size: 66 944 494 954 1924 1572 3341
Fragments arranged by size:
3341 1924 1572 954 944 494 66
ApaLI G'TGCA_C
Cuts at: 3301 7352 8508 3301
Size: 4051 1156 4088
Fragments arranged by size:
4088 4051 1156
ApoI r'AATT_y
Cuts at: 1493 2237 2398 2780 2873 3850 4102 6065 6145
Size: 744 161 382 93 977 252 1963 80
Cuts at: 6145 6178 6353 6544 6604 6830 6982 9091 1493
Size: 33 175 191 60 226 152 2109 1697
Fragments arranged by size:
2109 1963 1697 977 744 382 252 226
191 175 161 152 93 80 60 33
AseI AT'TA_AT
Cuts at: 160 1032 4371 7849 160
Size: 872 3339 3478 1606
Fragments arranged by size:
3478 3339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9295
AvaI C'yCGr_G
Cuts at: 1870 1943 6620 1870
Size: 73 4677 4545
Fragments arranged by size:
4677 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2961 2994 2961
Size: 33 9262
BamHI G'GATC_C
Cuts at: 5521 6625 6992 5521
Size: 1104 367 7824
Fragments arranged by size:
7824 1104 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3650 6616 8071 618
Size: 325 217 627 1863 2966 1455 1842
Fragments arranged by size:
2966 1863 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5327 6614 1391
Size: 3936 1287 4072
Fragments arranged by size:
4072 3936 1287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3671 928
Size: 971 1772 6552
Fragments arranged by size:
6552 1772 971
BbvCI CC'TCA_GC
Cuts at: 2619 2619
Size: 9295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5523 5557 7486 7520 1903
Size: 34 3586 34 1929 34 3678
Fragments arranged by size:
3678 3586 1929 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7187 8397 7187
Size: 1210 8085
BclI T'GATC_A
Cuts at: 1548 2293 5050 1548
Size: 745 2757 5793
Fragments arranged by size:
5793 2757 745
BfrBI ATG'CAT
Cuts at: 3723 4559 5492 6307 3723
Size: 836 933 815 6711
Fragments arranged by size:
6711 933 836 815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4607 7906 136
Size: 107 122 71 631 605 2935 3299 1525
Fragments arranged by size:
3299 2935 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4333 9290 4333
Size: 4957 4338
BlpI GC'TnA_GC
Cuts at: 4611 5726 5816 6712 4611
Size: 1115 90 896 7194
Fragments arranged by size:
7194 1115 896 90
Bme1580I G_kGCm'C
Cuts at: 1790 3305 7356 8512 1790
Size: 1515 4051 1156 2573
Fragments arranged by size:
4051 2573 1515 1156
BmgBI CAC'GTC
Cuts at: 1318 5358 1318
Size: 4040 5255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2911 3209 4222
Size: 416 53 83 399 24 1494 298 1013
Cuts at: 4222 6444 7026 7984 442
Size: 2222 582 958 1753
Fragments arranged by size:
2222 1753 1494 1013 958 582 416 399
298 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5241 5102
Size: 139 9156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3588 4451 7955 1239
Size: 2349 863 3504 2579
Fragments arranged by size:
3504 2579 2349 863
Bpu10I CC'TnA_GC
Cuts at: 2255 2619 3774 2255
Size: 364 1155 7776
Fragments arranged by size:
7776 1155 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5904 7420 8285 8583 5904
Size: 1516 865 298 6616
Fragments arranged by size:
6616 1516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7958 882
Size: 617 6459 2219
Fragments arranged by size:
6459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6592 7086 493
Size: 711 5388 494 2702
Fragments arranged by size:
5388 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3724 9289 3724
Size: 5565 3730
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5312 7102 7484 8119
Size: 53 83 186 936 3779 1790 382 635
Cuts at: 8119 275
Size: 1451
Fragments arranged by size:
3779 1790 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4264 7728 8126 8400 8547 8958 4264
Size: 3464 398 274 147 411 4601
Fragments arranged by size:
4601 3464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2914 2944 3059 3076 3089
Size: 30 165 30 1511 30 115 17 13
Cuts at: 3089 3106 3406 3436 5314 5344 1178
Size: 17 300 30 1878 30 5129
Fragments arranged by size:
5129 1878 1511 300 165 115 30 30
30 30 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3135 1186
Size: 1949 7346
BseYI C'CCAG_C
Cuts at: 3444 8498 8994 3444
Size: 5054 496 3745
Fragments arranged by size:
5054 3745 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2170 3495 3531 4828 1163
Size: 573 434 1325 36 1297 5630
Fragments arranged by size:
5630 1325 1297 573 434 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6745 7506 8534 1540
Size: 275 186 4744 761 1028 2301
Fragments arranged by size:
4744 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2927 3260 3272 3305 3768 5327 7356 7441 8512
Size: 333 12 33 463 1559 2029 85 1071
Cuts at: 8512 2927
Size: 3710
Fragments arranged by size:
3710 2029 1559 1071 463 333 85 33
12
BsmI GAATG_Cn'
Cuts at: 2374 3335 3620 3646 3751 3792 5466 6806 6899
Size: 961 285 26 105 41 1674 1340 93
Cuts at: 6899 2374
Size: 4770
Fragments arranged by size:
4770 1674 1340 961 285 105 93 41
26
BsmBI CGTCTCn'nnnn_
Cuts at: 4592 5319 4592
Size: 727 8568
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2927 3260 3272 3305 3768 5327 6614
Size: 399 1137 333 12 33 463 1559 1287
Cuts at: 6614 7356 7441 8512 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1559 1287 1137 1071 742 463 399
333 85 33 12
BspEI T'CCGG_A
Cuts at: 8958 8958
Size: 9295
BspHI T'CATG_A
Cuts at: 2833 5574 7184 8914 2833
Size: 2741 1610 1730 3214
Fragments arranged by size:
3214 2741 1730 1610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2621 2947 3785 4039 5113 6650 844
Size: 1205 572 326 838 254 1074 1537 3489
Fragments arranged by size:
3489 1537 1205 1074 838 572 326 254
BsrBI CCG'CTC
Cuts at: 1394 1823 7182 1394
Size: 429 5359 3507
Fragments arranged by size:
5359 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3500 5432 5601 5738 7790 7964 65
Size: 1183 2252 1932 169 137 2052 174 1396
Fragments arranged by size:
2252 2052 1932 1396 1183 174 169 137
BsrFI r'CCGG_y
Cuts at: 1700 1810 3294 5207 7939 8126 1700
Size: 110 1484 1913 2732 187 2869
Fragments arranged by size:
2869 2732 1913 1484 187 110
BsrGI T'GTAC_A
Cuts at: 96 3377 96
Size: 3281 6014
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9295
BssSI C'ACGA_G
Cuts at: 7355 8367 7355
Size: 1012 8283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3360 3666 3787 6148 6503 794
Size: 2566 306 121 2361 355 3586
Fragments arranged by size:
3586 2566 2361 355 306 121
BstBI TT'CG_AA
Cuts at: 2007 8099 2007
Size: 6092 3203
BstEII G'GTnAC_C
Cuts at: 1184 3075 3814 1184
Size: 1891 739 6665
Fragments arranged by size:
6665 1891 739
BstXI CCAn_nnnn'nTGG
Cuts at: 6528 6528
Size: 9295
BstYI r'GATC_y
Cuts at: 1972 2209 2320 3885 4333 4751 5521 6625 6992
Size: 237 111 1565 448 418 770 1104 367
Cuts at: 6992 7377 7394 8846 8932 8944 9290 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1565 1452 1104 770 448 418 385
367 346 237 111 86 17 12
BstZ17I GTA'TAC
Cuts at: 2601 2601
Size: 9295
Bsu36I CC'TnA_GG
Cuts at: 2850 3567 2850
Size: 717 8578
BtgI C'CryG_G
Cuts at: 513 1065 3080 3316 3341 5307 6428 513
Size: 552 2015 236 25 1966 1121 3380
Fragments arranged by size:
3380 2015 1966 1121 552 236 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3553 487
Size: 683 56 2327 6229
Fragments arranged by size:
6229 2327 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3036 3303 3305 3483 3535 4681 4854 5461
Size: 41 267 2 178 52 1146 173 607
Cuts at: 5461 6811 7605 7625 2995
Size: 1350 794 20 4665
Fragments arranged by size:
4665 1350 1146 794 607 267 178 173
52 41 20 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9260
DraI TTT'AAA
Cuts at: 3913 6599 3913
Size: 2686 6609
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 8302 809
Size: 507 4046 2940 1802
Fragments arranged by size:
4046 2940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5007 5376 6742
Size: 54 1425 50 275 18 3177 369 1366
Cuts at: 6742 7631 9260 8
Size: 889 1629 43
Fragments arranged by size:
3177 1629 1425 1366 889 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6742 1537
Size: 275 4930 4090
Fragments arranged by size:
4930 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2901 6311 7225 1353
Size: 1548 3410 914 3423
Fragments arranged by size:
3423 3410 1548 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7882 8256 8402 387
Size: 1064 138 6293 374 146 1280
Fragments arranged by size:
6293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5325 5325
Size: 9295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3588 4451 4638 5739 7358 7955 8742
Size: 393 1956 863 187 1101 1619 597 787
Cuts at: 8742 9010 1239
Size: 268 1524
Fragments arranged by size:
1956 1619 1524 1101 863 787 597 393
268 187
EcoNI CCTnn'n_nnAGG
Cuts at: 2714 3773 2714
Size: 1059 8236
EcoO109I rG'GnC_Cy
Cuts at: 2251 2845 3173 3462 5102 2251
Size: 594 328 289 1640 6444
Fragments arranged by size:
6444 1640 594 328 289
EcoRI G'AATT_C
Cuts at: 4102 6065 6604 6982 4102
Size: 1963 539 378 6415
Fragments arranged by size:
6415 1963 539 378
EcoRV GAT'ATC
Cuts at: 6243 6243
Size: 9295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3785 3785
Size: 9295
FspI TGC'GCA
Cuts at: 7801 7801
Size: 9295
HaeII r_GCGC'y
Cuts at: 1678 8442 1678
Size: 6764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 2636 2668 2782
Size: 32 135 32 884 32 148 32 114
Cuts at: 2782 2814 3059 3091 3791 3823 4277 4309 4574
Size: 32 245 32 700 32 454 32 265
Cuts at: 4574 4606 4614 4646 5163 5195 5378 5410 5500
Size: 32 8 32 517 32 183 32 90
Cuts at: 5500 5532 7936 7968 8010 8042 1373
Size: 32 2404 32 42 32 2626
Fragments arranged by size:
2626 2404 884 700 517 454 265 245
183 148 135 114 90 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4210 5128 6639 6885 669
Size: 988 281 2272 918 1511 246 3079
Fragments arranged by size:
3079 2272 1511 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 4145 748
Size: 3397 5898
HpaI GTT'AAC
Cuts at: 6885 6885
Size: 9295
KpnI G_GTAC'C
Cuts at: 6620 6620
Size: 9295
MfeI C'AATT_G
Cuts at: 6894 6894
Size: 9295
MluI A'CGCG_T
Cuts at: 9171 9171
Size: 9295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2237 2450 2526 3810 4057 4792 6177 8409
Size: 932 213 76 1284 247 735 1385 2232
Cuts at: 8409 8593 1305
Size: 184 2007
Fragments arranged by size:
2232 2007 1385 1284 932 735 247 213
184 76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5009 5378 10
Size: 54 1425 343 3177 369 3927
Fragments arranged by size:
3927 3177 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3100 3414 4265 5497 5798 5804 6170
Size: 648 1934 314 851 1232 301 6 366
Cuts at: 6170 7253 7612 7771 518
Size: 1083 359 159 2042
Fragments arranged by size:
2042 1934 1232 1083 851 648 366 359
314 301 159 6
MspA1I CmG'CkG
Cuts at: 1606 3003 3311 5114 6170 6711 7388 8536 8781
Size: 1397 308 1803 1056 541 677 1148 245
Cuts at: 8781 1606
Size: 2120
Fragments arranged by size:
2120 1803 1397 1148 1056 677 541 308
245
NaeI GCC'GGC
Cuts at: 3296 3296
Size: 9295
NcoI C'CATG_G
Cuts at: 513 1065 3080 3316 3341 5307 6428 513
Size: 552 2015 236 25 1966 1121 3380
Fragments arranged by size:
3380 2015 1966 1121 552 236 25
NdeI CA'TA_TG
Cuts at: 387 6138 6324 387
Size: 5751 186 3358
Fragments arranged by size:
5751 3358 186
NgoMIV G'CCGG_C
Cuts at: 3294 3294
Size: 9295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9295
NotI GC'GGCC_GC
Cuts at: 6742 6742
Size: 9295
NsiI A_TGCA'T
Cuts at: 3725 4561 5494 6309 3725
Size: 836 933 815 6711
Fragments arranged by size:
6711 933 836 815
NspI r_CATG'y
Cuts at: 1132 1229 2931 3105 5567 5797 6653 8198 1132
Size: 97 1702 174 2462 230 856 1545 2229
Fragments arranged by size:
2462 2229 1702 1545 856 230 174 97
PasI CC'CwG_GG
Cuts at: 1348 4531 1348
Size: 3183 6112
PciI A'CATG_T
Cuts at: 2927 3101 8194 2927
Size: 174 5093 4028
Fragments arranged by size:
5093 4028 174
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3221 1108
Size: 2113 7182
PmeI GTTT'AAAC
Cuts at: 6599 6599
Size: 9295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2946 3076 3108 3406 3438 7364 7396 8877
Size: 32 130 32 298 32 3926 32 1481
Cuts at: 8877 8909 2914
Size: 32 3300
Fragments arranged by size:
3926 3300 1481 298 130 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2845 3173 3462 5102 2251
Size: 594 328 289 1640 6444
Fragments arranged by size:
6444 1640 594 328 289
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9295
PsiI TTA'TAA
Cuts at: 4958 6865 4958
Size: 1907 7388
PstI C_TGCA'G
Cuts at: 830 3002 3310 4156 5110 6647 830
Size: 2172 308 846 954 1537 3478
Fragments arranged by size:
3478 2172 1537 954 846 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9295
PvuII CAG'CTG
Cuts at: 3311 6170 3311
Size: 2859 6436
SacI G_AGCT'C
Cuts at: 5327 5327
Size: 9295
SalI G'TCGA_C
Cuts at: 1936 6637 1936
Size: 4701 4594
SanDI GG'GwC_CC
Cuts at: 2845 2845
Size: 9295
SbfI CC_TGCA'GG
Cuts at: 6647 6647
Size: 9295
ScaI AGT'ACT
Cuts at: 7543 9255 7543
Size: 1712 7583
SfcI C'TryA_G
Cuts at: 826 1045 2384 2998 3306 4152 5106 6643 7778
Size: 219 1339 614 308 846 954 1537 1135
Cuts at: 7778 8459 8650 826
Size: 681 191 1471
Fragments arranged by size:
1537 1471 1339 1135 954 846 681 614
308 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9295
SmaI CCC'GGG
Cuts at: 1872 6622 1872
Size: 4750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5919 7399 8300 8562 820
Size: 197 926 3976 1480 901 262 1553
Fragments arranged by size:
3976 1553 1480 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6592 7086 493
Size: 6099 494 2702
Fragments arranged by size:
6099 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9295
SphI G_CATG'C
Cuts at: 1132 5567 6653 1132
Size: 4435 1086 3774
Fragments arranged by size:
4435 3774 1086
SspI AAT'ATT
Cuts at: 5 52 3202 6010 7219 5
Size: 47 3150 2808 1209 2081
Fragments arranged by size:
3150 2808 2081 1209 47
StuI AGG'CCT
Cuts at: 1517 4402 1517
Size: 2885 6410
StyI C'CwwG_G
Cuts at: 513 1065 3080 3316 3341 4978 5307 6428 6734
Size: 552 2015 236 25 1637 329 1121 306
Cuts at: 6734 513
Size: 3074
Fragments arranged by size:
3074 2015 1637 1121 552 329 306 236
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2747 2785 4544 7322 7339 7492 7677
Size: 1255 1167 38 1759 2778 17 153 185
Cuts at: 7677 8122 325
Size: 445 1498
Fragments arranged by size:
2778 1759 1498 1255 1167 445 185 153
38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2537 3052 3377
Size: 274 80 33 51 1443 560 515 325
Cuts at: 3377 3735 5865 7541 9253 96
Size: 358 2130 1676 1712 138
Fragments arranged by size:
2130 1712 1676 1443 560 515 358 325
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4113 4145 5314 5346 9255 9287 4113
Size: 32 1169 32 3909 32 4121
Fragments arranged by size:
4121 3909 1169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5909 1316
Size: 4593 4702
XbaI T'CTAG_A
Cuts at: 3979 6631 3979
Size: 2652 6643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 3826 4384 5305 2068
Size: 1758 558 921 6058
Fragments arranged by size:
6058 1758 921 558
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9295
XmaI C'CCGG_G
Cuts at: 1870 6620 1870
Size: 4750 4545
XmnI GAAnn'nnTTC
Cuts at: 2163 2372 5668 6179 6333 7424 2163
Size: 209 3296 511 154 1091 4034
Fragments arranged by size:
4034 3296 1091 511 209 154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7103 276
Size: 53 83 186 936 5569 2468
Fragments arranged by size:
5569 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BsiWI DraIII FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI