Restriction map for
pFN21ASDA0544
(Circular) (Six-base) MAPSORT of: pFN21ASDA0544.seq Check: 5555 from: 1 to: 6385
pFN21ASDA0544 6385 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3628 3628
Size: 6385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2466 3728 1937
Size: 529 1262 4594
Fragments arranged by size:
4594 1262 529
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 3028 4448 5832 6100 1632
Size: 558 838 1420 1384 268 1917
Fragments arranged by size:
1917 1420 1384 838 558 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 5284 6261 1203
Size: 4081 977 1327
Fragments arranged by size:
4081 1327 977
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 3289 5114 3289
Size: 1825 4560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6385
AlwNI CAG_nnn'CTG
Cuts at: 3067 4128 5700 3067
Size: 1061 1572 3752
Fragments arranged by size:
3752 1572 1061
ApaI G_GGCC'C
Cuts at: 2989 3651 2989
Size: 662 5723
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 2150 3694 3920 4072 6181 1493
Size: 657 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 657 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 3197 3555 3596 3710 1870
Size: 73 421 833 358 41 114 4545
Fragments arranged by size:
4545 833 421 358 114 73 41
BamHI G'GATC_C
Cuts at: 3715 4082 3715
Size: 367 6018
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3706 5161 618
Size: 325 217 627 1919 1455 1842
Fragments arranged by size:
1919 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2262 2483 2989 3028 3186 3651 3704
Size: 639 232 221 506 39 158 465 53
Cuts at: 3704 1391
Size: 4072
Fragments arranged by size:
4072 639 506 465 232 221 158 53
39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2273 3390 928
Size: 971 374 1117 3923
Fragments arranged by size:
3923 1117 971 374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2384 2418 4576 4610 1903
Size: 34 447 34 2158 34 3678
Fragments arranged by size:
3678 2158 447 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6385
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2411 3125 3351
Size: 107 122 71 631 605 739 714 226
Cuts at: 3351 4996 136
Size: 1645 1525
Fragments arranged by size:
1645 1525 739 714 631 605 226 122
107 71
BglII A'GATC_T
Cuts at: 2778 6380 2778
Size: 3602 2783
BlpI GC'TnA_GC
Cuts at: 3802 3802
Size: 6385
Bme1580I G_kGCm'C
Cuts at: 1790 2989 3651 4446 5602 1790
Size: 1199 662 795 1156 2573
Fragments arranged by size:
2573 1199 1156 795 662
BmgBI CAC'GTC
Cuts at: 1318 3442 1318
Size: 2124 4261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3470 4116 5074
Size: 416 53 83 399 24 2053 646 958
Cuts at: 5074 442
Size: 1753
Fragments arranged by size:
2053 1753 958 646 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3448 3205
Size: 243 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 3089 5045 1239
Size: 1163 687 1956 2579
Fragments arranged by size:
2579 1956 1163 687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4510 5375 5673 4510
Size: 865 298 5222
Fragments arranged by size:
5222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 2804 5048 882
Size: 617 993 312 2244 2219
Fragments arranged by size:
2244 2219 993 617 312
BsaAI yAC'GTr
Cuts at: 493 1204 2677 3682 4176 493
Size: 711 1473 1005 494 2702
Fragments arranged by size:
2702 1473 1005 711 494
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2397 3447 4192 4574
Size: 53 83 186 936 864 1050 745 382
Cuts at: 4574 5209 275
Size: 635 1451
Fragments arranged by size:
1451 1050 936 864 745 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2108 4818 5216 5490 5637 6048 2108
Size: 2710 398 274 147 411 2445
Fragments arranged by size:
2710 2445 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2637 2667 3104 3134 1178
Size: 30 165 30 1234 30 437 30 4429
Fragments arranged by size:
4429 1234 437 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2157 2456 3437 1186
Size: 971 299 981 4134
Fragments arranged by size:
4134 981 971 299
BseYI C'CCAG_C
Cuts at: 5588 6084 5588
Size: 496 5889
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5812
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3835 4596 5624 1540
Size: 275 186 1834 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2373 2682 3084 3603 4446 4531 5602 2373
Size: 309 402 519 843 85 1071 3156
Fragments arranged by size:
3156 1071 843 519 402 309 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2262 2373 2483 2682 2989 3028
Size: 399 240 232 111 110 199 307 39
Cuts at: 3028 3084 3186 3603 3651 3704 4446 4531 5602
Size: 56 102 417 48 53 742 85 1071
Cuts at: 5602 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 417 399 307 240 232
199 111 110 102 85 56 53 48
39
BspEI T'CCGG_A
Cuts at: 2108 6048 2108
Size: 3940 2445
BspHI T'CATG_A
Cuts at: 4274 6004 4274
Size: 1730 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2883 3628 3642 3740 844
Size: 2039 745 14 98 3489
Fragments arranged by size:
3489 2039 745 98 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4272 1394
Size: 429 2449 3507
Fragments arranged by size:
3507 2449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4880 5054 65
Size: 1183 3632 174 1396
Fragments arranged by size:
3632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2970 5029 5216 1700
Size: 110 1160 2059 187 2869
Fragments arranged by size:
2869 2059 1160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6385
BssSI C'ACGA_G
Cuts at: 2255 4445 5457 2255
Size: 2190 1012 3183
Fragments arranged by size:
3183 2190 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6385
BstBI TT'CG_AA
Cuts at: 2007 5189 2007
Size: 3182 3203
BstEII G'GTnAC_C
Cuts at: 1184 2174 1184
Size: 990 5395
BstYI r'GATC_y
Cuts at: 1972 2197 2778 2997 3715 4082 4467 4484 5936
Size: 225 581 219 718 367 385 17 1452
Cuts at: 5936 6022 6034 6380 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 718 581 385 367 346 225
219 86 17 12
BstZ17I GTA'TAC
Cuts at: 2467 2467
Size: 6385
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4876
Fragments arranged by size:
4876 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2032 487
Size: 683 56 806 4840
Fragments arranged by size:
4840 806 683 56
BtsI GCAGTG_nn'
Cuts at: 3901 4695 4715 3901
Size: 794 20 5571
Fragments arranged by size:
5571 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6350
DraI TTT'AAA
Cuts at: 2733 3097 3689 2733
Size: 364 592 5429
Fragments arranged by size:
5429 592 364
DraIII CAC_nnn'GTG
Cuts at: 3464 3464
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5392 809
Size: 507 4076 1802
Fragments arranged by size:
4076 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 2665 3126
Size: 54 1425 50 275 18 582 253 461
Cuts at: 3126 3231 3607 3832 4721 6350 8
Size: 105 376 225 889 1629 43
Fragments arranged by size:
1629 1425 889 582 461 376 275 253
225 105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3408 3513 4315 1353
Size: 2055 105 802 3423
Fragments arranged by size:
3423 2055 802 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4972 5346 5492 387
Size: 1064 138 3383 374 146 1280
Fragments arranged by size:
3383 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2402 3028 3089 4448 5045 5832
Size: 393 558 212 626 61 1359 597 787
Cuts at: 5832 6100 1239
Size: 268 1524
Fragments arranged by size:
1524 1359 787 626 597 558 393 268
212 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 3293 2771
Size: 522 5863
EcoO109I rG'GnC_Cy
Cuts at: 2986 3370 3552 3647 2986
Size: 384 182 95 5724
Fragments arranged by size:
5724 384 182 95
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 6385
FspI TGC'GCA
Cuts at: 4891 4891
Size: 6385
HaeII r_GCGC'y
Cuts at: 1678 3206 5532 1678
Size: 1528 2326 2531
Fragments arranged by size:
2531 2326 1528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3068 3100 3275 3307 3518
Size: 32 135 32 1496 32 175 32 211
Cuts at: 3518 3521 3550 3553 3644 3676 5026 5058 5100
Size: 3 29 3 91 32 1350 32 42
Cuts at: 5100 5132 1373
Size: 32 2626
Fragments arranged by size:
2626 1496 1350 211 175 135 91 42
32 32 32 32 32 32 32 29
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3729 3975 669
Size: 988 281 1791 246 3079
Fragments arranged by size:
3079 1791 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 5499 5683 1305
Size: 1590 2604 184 2007
Fragments arranged by size:
2604 2007 1590 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 2667 3128 10
Size: 54 1425 343 582 253 461 3267
Fragments arranged by size:
3267 1425 582 461 343 253 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4343 4702 4861 518
Size: 648 3177 359 159 2042
Fragments arranged by size:
3177 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3303 3801 4478 5626 5871 1606
Size: 1697 498 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 498 245
NaeI GCC'GGC
Cuts at: 2972 2972
Size: 6385
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4876
Fragments arranged by size:
4876 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NgoMIV G'CCGG_C
Cuts at: 2970 2970
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 3743 5288 1132
Size: 97 2514 1545 2229
Fragments arranged by size:
2514 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3643 1348
Size: 2295 4090
PciI A'CATG_T
Cuts at: 5284 5284
Size: 6385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 3067 3331 3464 1108
Size: 1524 435 264 133 4029
Fragments arranged by size:
4029 1524 435 264 133
PfoI T'CCnGG_A
Cuts at: 3330 3330
Size: 6385
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PmlI CAC'GTG
Cuts at: 2677 2677
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3552 3552
Size: 6385
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PspOMI G'GGCC_C
Cuts at: 2985 3647 2985
Size: 662 5723
PspXI vC'TCGA_Gb
Cuts at: 3197 3197
Size: 6385
PstI C_TGCA'G
Cuts at: 830 2775 2997 3585 3737 830
Size: 1945 222 588 152 3478
Fragments arranged by size:
3478 1945 588 222 152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 3303 3303
Size: 6385
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SanDI GG'GwC_CC
Cuts at: 3552 3552
Size: 6385
SbfI CC_TGCA'GG
Cuts at: 2775 3585 3737 2775
Size: 810 152 5423
Fragments arranged by size:
5423 810 152
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 2374 2489 2771 2993 3581 3733 4868
Size: 219 1329 115 282 222 588 152 1135
Cuts at: 4868 5549 5740 826
Size: 681 191 1471
Fragments arranged by size:
1471 1329 1135 681 588 282 222 219
191 152 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 2366 3712 1872
Size: 494 1346 4545
Fragments arranged by size:
4545 1346 494
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3197 4489 5390 5652 820
Size: 197 926 1254 1292 901 262 1553
Fragments arranged by size:
1553 1292 1254 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3682 4176 493
Size: 3189 494 2702
Fragments arranged by size:
3189 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 3743 1132
Size: 2611 3774
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6385
StyI C'CwwG_G
Cuts at: 513 1065 2022 3824 513
Size: 552 957 1802 3074
Fragments arranged by size:
3074 1802 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 4412 4429 4582 4767 5212 325
Size: 1255 1872 960 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 960 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4631 6343 96
Size: 274 80 33 51 1443 2654 1712 138
Fragments arranged by size:
2654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 3104 3136 6345 6377 2637
Size: 32 435 32 3209 32 2645
Fragments arranged by size:
3209 2645 435 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2756 3164 1316
Size: 1440 408 4537
Fragments arranged by size:
4537 1440 408
XbaI T'CTAG_A
Cuts at: 3721 3721
Size: 6385
XhoI C'TCGA_G
Cuts at: 1943 3197 1943
Size: 1254 5131
XmaI C'CCGG_G
Cuts at: 1870 2364 3710 1870
Size: 494 1346 4545
Fragments arranged by size:
4545 1346 494
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI