Restriction map for
pFN21ASDA0529
(Circular) (Six-base) MAPSORT of: pFN21ASDA0529.seq Check: 967 from: 1 to: 7585
pFN21ASDA0529 7585 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5395 278
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4928 1937
Size: 2991 4594
Acc65I G'GTAC_C
Cuts at: 2112 4906 2112
Size: 2794 4791
AclI AA'CG_TT
Cuts at: 1563 5712 6085 1563
Size: 4149 373 3063
Fragments arranged by size:
4149 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2325 2512 3762 3828 3837 3996 4365 4704
Size: 693 187 1250 66 9 159 369 339
Cuts at: 4704 4773 5648 7032 7300 1632
Size: 69 875 1384 268 1917
Fragments arranged by size:
1917 1384 1250 875 693 369 339 268
187 159 69 66 9
AflII C'TTAA_G
Cuts at: 820 1017 2258 820
Size: 197 1241 6147
Fragments arranged by size:
6147 1241 197
AflIII A'CryG_T
Cuts at: 1203 2825 6484 7461 1203
Size: 1622 3659 977 1327
Fragments arranged by size:
3659 1622 1327 977
AgeI A'CCGG_T
Cuts at: 6416 6416
Size: 7585
AhdI GACnn_n'nnGTC
Cuts at: 6314 6314
Size: 7585
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4567 1184
Size: 3383 4202
AlwNI CAG_nnn'CTG
Cuts at: 5328 6900 5328
Size: 1572 6013
ApaLI G'TGCA_C
Cuts at: 5642 6798 5642
Size: 1156 6429
ApoI r'AATT_y
Cuts at: 1493 2914 3656 4523 4894 5120 5272 7381 1493
Size: 1421 742 867 371 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 867 742 371 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3852 4533 6139 160
Size: 872 2820 681 1606 1606
Fragments arranged by size:
2820 1606 1606 872 681
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
AvaI C'yCGr_G
Cuts at: 1870 1943 3667 4910 1870
Size: 73 1724 1243 4545
Fragments arranged by size:
4545 1724 1243 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4473 4506 4473
Size: 33 7552
BamHI G'GATC_C
Cuts at: 3385 3821 4195 4915 5282 3385
Size: 436 374 720 367 5688
Fragments arranged by size:
5688 720 436 374 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 4906 6361 618
Size: 325 217 627 325 2794 1455 1842
Fragments arranged by size:
2794 1842 1455 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 4904 1391
Size: 3513 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2720 3750 4692 928
Size: 971 821 1030 942 3821
Fragments arranged by size:
3821 1030 971 942 821
BbvCI CC'TCA_GC
Cuts at: 4236 4236
Size: 7585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5776 5810 1903
Size: 34 3839 34 3678
Fragments arranged by size:
3839 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2214 4021 4474 5477 6687 2214
Size: 1807 453 1003 1210 3112
Fragments arranged by size:
3112 1807 1210 1003 453
BclI T'GATC_A
Cuts at: 1548 2970 1548
Size: 1422 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2802 3870 6196
Size: 107 122 71 631 605 1130 1068 2326
Cuts at: 6196 136
Size: 1525
Fragments arranged by size:
2326 1525 1130 1068 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3445 4172 7580 3445
Size: 727 3408 3450
Fragments arranged by size:
3450 3408 727
BlpI GC'TnA_GC
Cuts at: 5002 5002
Size: 7585
Bme1580I G_kGCm'C
Cuts at: 1790 5646 6802 1790
Size: 3856 1156 2573
Fragments arranged by size:
3856 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 5316 6274
Size: 416 53 83 399 24 828 3071 958
Cuts at: 6274 442
Size: 1753
Fragments arranged by size:
3071 1753 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4404 6245 1239
Size: 3165 1841 2579
Fragments arranged by size:
3165 2579 1841
Bpu10I CC'TnA_GC
Cuts at: 4157 4236 4157
Size: 79 7506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4424 5710 6575 6873 3790
Size: 634 1286 865 298 4502
Fragments arranged by size:
4502 1286 865 634 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2543 6248 882
Size: 617 1044 3705 2219
Fragments arranged by size:
3705 2219 1044 617
BsaAI yAC'GTr
Cuts at: 493 1204 2826 3628 4882 5376 493
Size: 711 1622 802 1254 494 2702
Fragments arranged by size:
2702 1622 1254 802 711 494
BsaBI GATnn'nnATC
Cuts at: 2898 4194 7579 2898
Size: 1296 3385 2904
Fragments arranged by size:
3385 2904 1296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5392 5774 6409 275
Size: 53 83 186 936 3859 382 635 1451
Fragments arranged by size:
3859 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2094 6018 6416 6690 6837 7248 2094
Size: 3924 398 274 147 411 2431
Fragments arranged by size:
3924 2431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7360
Fragments arranged by size:
7360 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4005 4614 1186
Size: 2819 609 4157
Fragments arranged by size:
4157 2819 609
BseYI C'CCAG_C
Cuts at: 2055 6788 7284 2055
Size: 4733 496 2356
Fragments arranged by size:
4733 2356 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7012
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5035 5796 6824 1540
Size: 275 186 3034 761 1028 2301
Fragments arranged by size:
3034 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3627 5646 5731 6802 3627
Size: 2019 85 1071 4410
Fragments arranged by size:
4410 2019 1071 85
BsmI GAATG_Cn'
Cuts at: 5096 5189 5096
Size: 93 7492
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3627 4904 5646 5731 6802 1391
Size: 399 1837 1277 742 85 1071 2174
Fragments arranged by size:
2174 1837 1277 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2094 7248 2094
Size: 5154 2431
BspHI T'CATG_A
Cuts at: 2832 5474 7204 2832
Size: 2642 1730 3213
Fragments arranged by size:
3213 2642 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3490 4535 4940 844
Size: 2646 1045 405 3489
Fragments arranged by size:
3489 2646 1045 405
BsrBI CCG'CTC
Cuts at: 1394 1823 2039 5472 1394
Size: 429 216 3433 3507
Fragments arranged by size:
3507 3433 429 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 3335 4837 6080 6254 65
Size: 1183 2087 1502 1243 174 1396
Fragments arranged by size:
2087 1502 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6229 6416 1700
Size: 110 4419 187 2869
Fragments arranged by size:
4419 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3452 96
Size: 3356 4229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7585
BssSI C'ACGA_G
Cuts at: 5645 6657 5645
Size: 1012 6573
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3464 794
Size: 2670 4915
BstBI TT'CG_AA
Cuts at: 2007 6389 2007
Size: 4382 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7585
BstXI CCAn_nnnn'nTGG
Cuts at: 3686 3686
Size: 7585
BstYI r'GATC_y
Cuts at: 1972 2044 3385 3445 3821 4172 4195 4348 4915
Size: 72 1341 60 376 351 23 153 567
Cuts at: 4915 5282 5667 5684 7136 7222 7234 7580 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1341 567 385 376 367 351
346 153 86 72 60 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2929 2929
Size: 7585
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6076
Fragments arranged by size:
6076 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2053 487
Size: 683 56 827 6019
Fragments arranged by size:
6019 827 683 56
BtsI GCAGTG_nn'
Cuts at: 2398 3829 4674 5101 5895 5915 2398
Size: 1431 845 427 794 20 4068
Fragments arranged by size:
4068 1431 845 794 427 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7550
DraI TTT'AAA
Cuts at: 3216 4889 3216
Size: 1673 5912
DraIII CAC_nnn'GTG
Cuts at: 3896 3896
Size: 7585
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4667 6592 809
Size: 507 3351 1925 1802
Fragments arranged by size:
3351 1925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2681 5032 5921
Size: 54 1425 50 275 18 851 2351 889
Cuts at: 5921 7550 8
Size: 1629 43
Fragments arranged by size:
2351 1629 1425 889 851 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5032 1537
Size: 275 3220 4090
Fragments arranged by size:
4090 3220 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4724 5515 1353
Size: 3371 791 3423
Fragments arranged by size:
3423 3371 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2049 2057 6172 6546 6692
Size: 1064 138 453 7 8 4115 374 146
Cuts at: 6692 387
Size: 1280
Fragments arranged by size:
4115 1280 1064 453 374 146 138 8
7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2325 2512 3762 3828 3837 3996 4365
Size: 393 693 187 1250 66 9 159 369
Cuts at: 4365 4404 4704 4773 5648 6245 7032 7300 1239
Size: 39 300 69 875 597 787 268 1524
Fragments arranged by size:
1524 1250 875 787 693 597 393 369
300 268 187 159 69 66 39 9
EcoO109I rG'GnC_Cy
Cuts at: 2690 4547 2690
Size: 1857 5728
EcoRI G'AATT_C
Cuts at: 4523 4894 5272 4523
Size: 371 378 6836
Fragments arranged by size:
6836 378 371
EcoRV GAT'ATC
Cuts at: 3062 4105 3062
Size: 1043 6542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2709 3767 2709
Size: 1058 6527
FspI TGC'GCA
Cuts at: 6091 6091
Size: 7585
HaeII r_GCGC'y
Cuts at: 1678 6732 1678
Size: 5054 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2176 2208 2237 2269 2332
Size: 32 135 32 604 32 29 32 63
Cuts at: 2332 2364 3911 3943 4157 4189 4394 4426 6226
Size: 32 1547 32 214 32 205 32 1800
Cuts at: 6226 6258 6300 6332 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1800 1547 604 214 205 135 63
42 32 32 32 32 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 4043 4929 5175 669
Size: 988 281 2105 886 246 3079
Fragments arranged by size:
3079 2105 988 886 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7585
HpaI GTT'AAC
Cuts at: 5175 5175
Size: 7585
KpnI G_GTAC'C
Cuts at: 2116 4910 2116
Size: 2794 4791
MfeI C'AATT_G
Cuts at: 2502 5184 2502
Size: 2682 4903
MluI A'CGCG_T
Cuts at: 7461 7461
Size: 7585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2291 3967 4458 4500 4602 6699 6883 1305
Size: 986 1676 491 42 102 2097 184 2007
Fragments arranged by size:
2097 2007 1676 986 491 184 102 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2683 10
Size: 54 1425 343 851 4912
Fragments arranged by size:
4912 1425 851 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 4257 5543 5902 6061 518
Size: 648 1173 1918 1286 359 159 2042
Fragments arranged by size:
2042 1918 1286 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2328 4793 5001 5678 6826 7071 1606
Size: 453 269 2465 208 677 1148 245 2120
Fragments arranged by size:
2465 2120 1148 677 453 269 245 208
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6076
Fragments arranged by size:
6076 957 552
NdeI CA'TA_TG
Cuts at: 387 4711 387
Size: 4324 3261
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7585
NotI GC'GGCC_GC
Cuts at: 5032 5032
Size: 7585
NruI TCG'CGA
Cuts at: 2072 2072
Size: 7585
NspI r_CATG'y
Cuts at: 1132 1229 4943 6488 1132
Size: 97 3714 1545 2229
Fragments arranged by size:
3714 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 4533 4533
Size: 7585
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7585
PciI A'CATG_T
Cuts at: 6484 6484
Size: 7585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2559 1108
Size: 1451 6134
PfoI T'CCnGG_A
Cuts at: 2206 2206
Size: 7585
PmeI GTTT'AAAC
Cuts at: 4889 4889
Size: 7585
PmlI CAC'GTG
Cuts at: 3628 3628
Size: 7585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5654 5686 7167 7199 5654
Size: 32 1481 32 6040
Fragments arranged by size:
6040 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2690 4547 2690
Size: 1857 5728
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7585
PsiI TTA'TAA
Cuts at: 2748 3756 5155 2748
Size: 1008 1399 5178
Fragments arranged by size:
5178 1399 1008
PspXI vC'TCGA_Gb
Cuts at: 3667 3667
Size: 7585
PstI C_TGCA'G
Cuts at: 830 3487 4937 830
Size: 2657 1450 3478
Fragments arranged by size:
3478 2657 1450
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7585
PvuII CAG'CTG
Cuts at: 2328 4793 2328
Size: 2465 5120
SalI G'TCGA_C
Cuts at: 1936 4927 1936
Size: 2991 4594
SbfI CC_TGCA'GG
Cuts at: 4937 4937
Size: 7585
ScaI AGT'ACT
Cuts at: 4425 5833 7545 4425
Size: 1408 1712 4465
Fragments arranged by size:
4465 1712 1408
SexAI A'CCwGG_T
Cuts at: 2108 2108
Size: 7585
SfcI C'TryA_G
Cuts at: 826 1045 3483 4933 6068 6749 6940 826
Size: 219 2438 1450 1135 681 191 1471
Fragments arranged by size:
2438 1471 1450 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2802 2802
Size: 7585
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7585
SmaI CCC'GGG
Cuts at: 1872 4912 1872
Size: 3040 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 3667 3769 4439 5689 6590
Size: 197 926 315 1409 102 670 1250 901
Cuts at: 6590 6852 820
Size: 262 1553
Fragments arranged by size:
1553 1409 1250 926 901 670 315 262
197 102
SnaBI TAC'GTA
Cuts at: 493 4882 5376 493
Size: 4389 494 2702
Fragments arranged by size:
4389 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7585
SphI G_CATG'C
Cuts at: 1132 4943 1132
Size: 3811 3774
SspI AAT'ATT
Cuts at: 5 52 3534 3850 5509 5
Size: 47 3482 316 1659 2081
Fragments arranged by size:
3482 2081 1659 316 47
StuI AGG'CCT
Cuts at: 1517 2796 1517
Size: 1279 6306
StyI C'CwwG_G
Cuts at: 513 1065 2022 2635 2684 3360 5024 513
Size: 552 957 613 49 676 1664 3074
Fragments arranged by size:
3074 1664 957 676 613 552 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 4785 5612 5629 5782 5967 6412
Size: 1255 489 2716 827 17 153 185 445
Cuts at: 6412 325
Size: 1498
Fragments arranged by size:
2716 1498 1255 827 489 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3408 3452 4019
Size: 274 80 33 51 1443 1431 44 567
Cuts at: 4019 4423 4432 4714 5831 7543 96
Size: 404 9 282 1117 1712 138
Fragments arranged by size:
1712 1443 1431 1117 567 404 282 274
138 80 51 44 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7545 7577 7545
Size: 32 7553
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7585
XbaI T'CTAG_A
Cuts at: 4921 4921
Size: 7585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2307 3851 4396 2307
Size: 1544 545 5496
Fragments arranged by size:
5496 1544 545
XhoI C'TCGA_G
Cuts at: 1943 3667 1943
Size: 1724 5861
XmaI C'CCGG_G
Cuts at: 1870 4910 1870
Size: 3040 4545
XmnI GAAnn'nnTTC
Cuts at: 3226 5714 3226
Size: 2488 5097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5393 276
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NsiI PspOMI PsrI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI