Restriction map for
pFN21ASDA0525
(Circular) (Six-base) MAPSORT of: pFN21ASDA0525.seq Check: 7560 from: 1 to: 7573
pFN21ASDA0525 7573 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 2328
Size: 7573
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 4317 4916 1937
Size: 1344 1036 599 4594
Fragments arranged by size:
4594 1344 1036 599
Acc65I G'GTAC_C
Cuts at: 3702 4894 3702
Size: 1192 6381
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2727 4690 4869 5636 7020 7288 1632
Size: 516 579 1963 179 767 1384 268 1917
Fragments arranged by size:
1963 1917 1384 767 579 516 268 179
AfeI AGC'GCT
Cuts at: 3382 3382
Size: 7573
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 6472 7449 1203
Size: 5269 977 1327
Fragments arranged by size:
5269 1327 977
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 2965 6302 2965
Size: 3337 4236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3493 1184
Size: 2309 5264
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4203 4235 4203
Size: 32 7541
AlwNI CAG_nnn'CTG
Cuts at: 2389 3071 5316 6888 2389
Size: 682 2245 1572 3074
Fragments arranged by size:
3074 2245 1572 682
ApaI G_GGCC'C
Cuts at: 4351 4351
Size: 7573
ApaLI G'TGCA_C
Cuts at: 4232 5630 6786 4232
Size: 1398 1156 5019
Fragments arranged by size:
5019 1398 1156
ApoI r'AATT_y
Cuts at: 1493 2894 3161 3262 3818 4427 4524 4795 4882
Size: 1401 267 101 556 609 97 271 87
Cuts at: 4882 5108 5260 7369 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1401 609 556 271 267 226
152 101 97 87
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 2429 4898 1870
Size: 73 486 2469 4545
Fragments arranged by size:
4545 2469 486 73
BamHI G'GATC_C
Cuts at: 4903 5270 4903
Size: 367 7206
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2658 3686 3702 4894 6349
Size: 325 217 627 871 1028 16 1192 1455
Cuts at: 6349 618
Size: 1842
Fragments arranged by size:
1842 1455 1192 1028 871 627 325 217
16
BanII G_rGCy'C
Cuts at: 1391 2436 2451 3680 4184 4351 4892 1391
Size: 1045 15 1229 504 167 541 4072
Fragments arranged by size:
4072 1229 1045 541 504 167 15
BbeI G_GCGC'C
Cuts at: 3690 3690
Size: 7573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2727 3030 928
Size: 971 828 303 5471
Fragments arranged by size:
5471 971 828 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5764 5798 1903
Size: 34 3827 34 3678
Fragments arranged by size:
3827 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2541 2934 4550 4859 5465 6675 2541
Size: 393 1616 309 606 1210 3439
Fragments arranged by size:
3439 1616 1210 606 393 309
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7573
BfrBI ATG'CAT
Cuts at: 3950 3950
Size: 7573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6184 136
Size: 107 122 71 631 605 4512 1525
Fragments arranged by size:
4512 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2941 7568 2941
Size: 4627 2946
BlpI GC'TnA_GC
Cuts at: 4990 4990
Size: 7573
Bme1580I G_kGCm'C
Cuts at: 1790 4236 4351 5634 6790 1790
Size: 2446 115 1283 1156 2573
Fragments arranged by size:
2573 2446 1283 1156 115
BmgBI CAC'GTC
Cuts at: 1318 4321 1318
Size: 3003 4570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 2995 3708
Size: 416 53 83 399 24 778 800 713
Cuts at: 3708 4544 5304 6262 442
Size: 836 760 958 1753
Fragments arranged by size:
1753 958 836 800 778 760 713 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2376 3401 6233 1239
Size: 1137 1025 2832 2579
Fragments arranged by size:
2832 2579 1137 1025
Bpu10I CC'TnA_GC
Cuts at: 3365 4142 3365
Size: 777 6796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4324 5698 6563 6861 4324
Size: 1374 865 298 5036
Fragments arranged by size:
5036 1374 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6236 882
Size: 617 4737 2219
Fragments arranged by size:
4737 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4870 5364 493
Size: 711 3666 494 2702
Fragments arranged by size:
3666 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3687 5380 5762 6397
Size: 53 83 186 936 2154 1693 382 635
Cuts at: 6397 275
Size: 1451
Fragments arranged by size:
2154 1693 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6006 6404 6678 6825 7236 6006
Size: 398 274 147 411 6343
Fragments arranged by size:
6343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2805 2835 3161 3191 3399
Size: 30 165 30 1402 30 326 30 208
Cuts at: 3399 3429 4203 4233 1178
Size: 30 774 30 4518
Fragments arranged by size:
4518 1402 774 326 208 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4211 1186
Size: 3025 4548
BseYI C'CCAG_C
Cuts at: 4365 6776 7272 4365
Size: 2411 496 4666
Fragments arranged by size:
4666 2411 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7000
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5023 5784 6812 1540
Size: 275 186 3022 761 1028 2301
Fragments arranged by size:
3022 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2753 3666 3789 4236 4358 5634 5719 6790
Size: 627 913 123 447 122 1276 85 1071
Cuts at: 6790 2126
Size: 2909
Fragments arranged by size:
2909 1276 1071 913 627 447 123 122
85
BsmI GAATG_Cn'
Cuts at: 5084 5177 5084
Size: 93 7480
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2436 2451 2753 3666 3680 3789
Size: 399 336 310 15 302 913 14 109
Cuts at: 3789 4184 4236 4351 4358 4892 5634 5719 6790
Size: 395 52 115 7 534 742 85 1071
Cuts at: 6790 1391
Size: 2174
Fragments arranged by size:
2174 1071 913 742 534 399 395 336
310 302 115 109 85 52 15 14
7
BspEI T'CCGG_A
Cuts at: 7236 7236
Size: 7573
BspHI T'CATG_A
Cuts at: 5462 7192 5462
Size: 1730 5843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2374 2620 4928 844
Size: 1484 46 246 2308 3489
Fragments arranged by size:
3489 2308 1484 246 46
BsrBI CCG'CTC
Cuts at: 1394 1823 5460 1394
Size: 429 3637 3507
Fragments arranged by size:
3637 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3249 6068 6242 65
Size: 1183 2001 2819 174 1396
Fragments arranged by size:
2819 2001 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6217 6404 1700
Size: 110 4407 187 2869
Fragments arranged by size:
4407 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3406 3646 4603 96
Size: 3310 240 957 3066
Fragments arranged by size:
3310 3066 957 240
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7573
BssSI C'ACGA_G
Cuts at: 5633 6645 5633
Size: 1012 6561
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2864 2873 4201 4337 794
Size: 2070 9 1328 136 4030
Fragments arranged by size:
4030 2070 1328 136 9
BstBI TT'CG_AA
Cuts at: 2007 6377 2007
Size: 4370 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7573
BstXI CCAn_nnnn'nTGG
Cuts at: 2166 3101 3166 4569 2166
Size: 935 65 1403 5170
Fragments arranged by size:
5170 1403 935 65
BstYI r'GATC_y
Cuts at: 1972 2941 3306 4903 5270 5655 5672 7124 7210
Size: 969 365 1597 367 385 17 1452 86
Cuts at: 7210 7222 7568 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1597 1452 969 385 367 365 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2342 2403 2342
Size: 61 7512
BtgI C'CryG_G
Cuts at: 513 1065 2022 2500 513
Size: 552 957 478 5586
Fragments arranged by size:
5586 957 552 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6834
Fragments arranged by size:
6834 683 56
BtsI GCAGTG_nn'
Cuts at: 2417 2574 3596 4338 5089 5883 5903 2417
Size: 157 1022 742 751 794 20 4087
Fragments arranged by size:
4087 1022 794 751 742 157 20
ClaI AT'CG_AT
Cuts at: 1554 3757 1554
Size: 2203 5370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7538
DraI TTT'AAA
Cuts at: 3261 3394 3894 4877 3261
Size: 133 500 983 5957
Fragments arranged by size:
5957 983 500 133
DraIII CAC_nnn'GTG
Cuts at: 3277 3277
Size: 7573
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6580 809
Size: 507 5264 1802
Fragments arranged by size:
5264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5020 5909 7538
Size: 54 1425 50 275 18 3190 889 1629
Cuts at: 7538 8
Size: 43
Fragments arranged by size:
3190 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5020 1537
Size: 275 3208 4090
Fragments arranged by size:
4090 3208 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2641 2892 3795 4683 5503 1353
Size: 1288 251 903 888 820 3423
Fragments arranged by size:
3423 1288 903 888 820 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6160 6534 6680 387
Size: 1064 138 4571 374 146 1280
Fragments arranged by size:
4571 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2376 2727 3401 4690 4869 5636
Size: 393 516 228 351 674 1289 179 767
Cuts at: 5636 6233 7020 7288 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1289 787 767 674 597 516 393
351 268 228 179
EcoNI CCTnn'n_nnAGG
Cuts at: 2345 2345
Size: 7573
EcoO109I rG'GnC_Cy
Cuts at: 2386 3451 4147 4347 2386
Size: 1065 696 200 5612
Fragments arranged by size:
5612 1065 696 200
EcoRI G'AATT_C
Cuts at: 4882 5260 4882
Size: 378 7195
EcoRV GAT'ATC
Cuts at: 2328 2328
Size: 7573
FspI TGC'GCA
Cuts at: 6079 6079
Size: 7573
HaeII r_GCGC'y
Cuts at: 1678 3384 3690 6720 1678
Size: 1706 306 3030 2531
Fragments arranged by size:
3030 2531 1706 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2740 2771 2772
Size: 32 135 32 779 32 357 31 1
Cuts at: 2772 2803 2951 2983 3161 3193 4166 4198 6214
Size: 31 148 32 178 32 973 32 2016
Cuts at: 6214 6246 6288 6320 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2016 973 779 357 178 148 135
42 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3527 4318 4917 5163 669
Size: 988 281 1589 791 599 246 3079
Fragments arranged by size:
3079 1589 988 791 599 281 246
HindIII A'AGCT_T
Cuts at: 748 3053 4460 4483 4832 748
Size: 2305 1407 23 349 3489
Fragments arranged by size:
3489 2305 1407 349 23
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KasI G'GCGC_C
Cuts at: 3686 3686
Size: 7573
KpnI G_GTAC'C
Cuts at: 3706 4898 3706
Size: 1192 6381
MfeI C'AATT_G
Cuts at: 4755 5172 4755
Size: 417 7156
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3190 3768 4763 4771 6687 6871 1305
Size: 1885 578 995 8 1916 184 2007
Fragments arranged by size:
2007 1916 1885 995 578 184 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5751
Fragments arranged by size:
5751 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 3065 3629 4646 5531 5890 6049
Size: 648 1564 335 564 1017 885 359 159
Cuts at: 6049 518
Size: 2042
Fragments arranged by size:
2042 1564 1017 885 648 564 359 335
159
MspA1I CmG'CkG
Cuts at: 1606 4989 5666 6814 7059 1606
Size: 3383 677 1148 245 2120
Fragments arranged by size:
3383 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3687 3687
Size: 7573
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6064
Fragments arranged by size:
6064 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NsiI A_TGCA'T
Cuts at: 3952 3952
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 3716 4931 6476 1132
Size: 97 2487 1215 1545 2229
Fragments arranged by size:
2487 2229 1545 1215 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7573
PciI A'CATG_T
Cuts at: 6472 6472
Size: 7573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3085 1108
Size: 1977 5596
PfoI T'CCnGG_A
Cuts at: 2388 3309 2388
Size: 921 6652
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4203 4235 5642 5674 7155 7187 4203
Size: 32 1407 32 1481 32 4589
Fragments arranged by size:
4589 1481 1407 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 3451 4147 2386
Size: 1065 696 5812
Fragments arranged by size:
5812 1065 696
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7573
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 4347 4347
Size: 7573
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2532 2564 2532
Size: 32 7541
PstI C_TGCA'G
Cuts at: 830 2325 2385 2581 3603 4442 4925 830
Size: 1495 60 196 1022 839 483 3478
Fragments arranged by size:
3478 1495 1022 839 483 196 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
SalI G'TCGA_C
Cuts at: 1936 4316 4915 1936
Size: 2380 599 4594
Fragments arranged by size:
4594 2380 599
SbfI CC_TGCA'GG
Cuts at: 2385 4925 2385
Size: 2540 5033
ScaI AGT'ACT
Cuts at: 4408 5821 7533 4408
Size: 1413 1712 4448
Fragments arranged by size:
4448 1712 1413
SexAI A'CCwGG_T
Cuts at: 3108 3108
Size: 7573
SfcI C'TryA_G
Cuts at: 826 1045 2321 2381 2521 2577 3483 3599 4438
Size: 219 1276 60 140 56 906 116 839
Cuts at: 4438 4921 6056 6737 6928 826
Size: 483 1135 681 191 1471
Fragments arranged by size:
1471 1276 1135 906 839 681 483 219
191 140 116 60 56
SfoI GGC'GCC
Cuts at: 3688 3688
Size: 7573
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 4900 1872
Size: 3028 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4303 5677 6578 6840 820
Size: 197 926 2360 1374 901 262 1553
Fragments arranged by size:
2360 1553 1374 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4870 5364 493
Size: 4377 494 2702
Fragments arranged by size:
4377 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 4931 1132
Size: 3799 3774
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 3992 4124 1517
Size: 2475 132 4966
Fragments arranged by size:
4966 2475 132
StyI C'CwwG_G
Cuts at: 513 1065 2022 2165 2529 2654 3993 4326 5012
Size: 552 957 143 364 125 1339 333 686
Cuts at: 5012 513
Size: 3074
Fragments arranged by size:
3074 1339 957 686 552 364 333 143
125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5600 5617 5770 5955 6400 325
Size: 1255 4020 17 153 185 445 1498
Fragments arranged by size:
4020 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3406 3646 4008
Size: 274 80 33 51 1443 1429 240 362
Cuts at: 4008 4406 4603 5819 7531 96
Size: 398 197 1216 1712 138
Fragments arranged by size:
1712 1443 1429 1216 398 362 274 240
197 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7533 7565 7533
Size: 32 7541
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7573
XbaI T'CTAG_A
Cuts at: 2890 3518 4909 2890
Size: 628 1391 5554
Fragments arranged by size:
5554 1391 628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 3098 4639 2680
Size: 418 1541 5614
Fragments arranged by size:
5614 1541 418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7573
XmaI C'CCGG_G
Cuts at: 1870 4898 1870
Size: 3028 4545
XmnI GAAnn'nnTTC
Cuts at: 4461 5702 4461
Size: 1241 6332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I
EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspXI PvuII RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI