Restriction map for
pFN21ASDA0517
(Circular) (Six-base) MAPSORT of: pFN21ASDA0517.seq Check: 3745 from: 1 to: 7042
pFN21ASDA0517 7042 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4852 278
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4385 1937
Size: 2448 4594
Acc65I G'GTAC_C
Cuts at: 3533 4363 3533
Size: 830 6212
AclI AA'CG_TT
Cuts at: 1563 5169 5542 1563
Size: 3606 373 3063
Fragments arranged by size:
3606 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 3047 3554 4254 5105 6489 6757 1632
Size: 1004 411 507 700 851 1384 268 1917
Fragments arranged by size:
1917 1384 1004 851 700 507 411 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6845
AflIII A'CryG_T
Cuts at: 1203 5941 6918 1203
Size: 4738 977 1327
Fragments arranged by size:
4738 1327 977
AgeI A'CCGG_T
Cuts at: 5873 5873
Size: 7042
AhdI GACnn_n'nnGTC
Cuts at: 5771 5771
Size: 7042
AleI CACnn'nnGTG
Cuts at: 1166 2031 2429 3716 1166
Size: 865 398 1287 4492
Fragments arranged by size:
4492 1287 865 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2076 2780 4128 1184
Size: 892 704 1348 4098
Fragments arranged by size:
4098 1348 892 704
AlwNI CAG_nnn'CTG
Cuts at: 2164 2426 4298 4785 6357 2164
Size: 262 1872 487 1572 2849
Fragments arranged by size:
2849 1872 1572 487 262
ApaLI G'TGCA_C
Cuts at: 2524 5099 6255 2524
Size: 2575 1156 3311
Fragments arranged by size:
3311 2575 1156
ApoI r'AATT_y
Cuts at: 1493 2483 3797 4064 4351 4577 4729 6838 1493
Size: 990 1314 267 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 990 287 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3605 5596 160
Size: 872 2573 1991 1606
Fragments arranged by size:
2573 1991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 3047 3424 4367 1870
Size: 73 369 735 377 943 4545
Fragments arranged by size:
4545 943 735 377 369 73
BamHI G'GATC_C
Cuts at: 4179 4372 4739 4179
Size: 193 367 6482
Fragments arranged by size:
6482 367 193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2238 3263 3533 4363
Size: 325 217 627 330 121 1025 270 830
Cuts at: 4363 5818 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1025 830 627 330 325 270
217 121
BanII G_rGCy'C
Cuts at: 1391 2736 4361 1391
Size: 1345 1625 4072
Fragments arranged by size:
4072 1625 1345
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 2747 3516 928
Size: 971 498 350 769 4454
Fragments arranged by size:
4454 971 769 498 350
BbvCI CC'TCA_GC
Cuts at: 2397 2397
Size: 7042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5233 5267 1903
Size: 34 3296 34 3678
Fragments arranged by size:
3678 3296 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2821 4934 6144 2821
Size: 2113 1210 3719
Fragments arranged by size:
3719 2113 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7042
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4254 5653 136
Size: 107 122 71 631 605 2582 1399 1525
Fragments arranged by size:
2582 1525 1399 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7037 7037
Size: 7042
BlpI GC'TnA_GC
Cuts at: 4459 4459
Size: 7042
Bme1580I G_kGCm'C
Cuts at: 1790 2528 3867 5103 6259 1790
Size: 738 1339 1236 1156 2573
Fragments arranged by size:
2573 1339 1236 1156 738
BmgBI CAC'GTC
Cuts at: 1318 2368 3439 1318
Size: 1050 1071 4921
Fragments arranged by size:
4921 1071 1050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2279 3726 4174
Size: 416 53 83 399 24 862 1447 448
Cuts at: 4174 4773 5731 442
Size: 599 958 1753
Fragments arranged by size:
1753 1447 958 862 599 448 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2367 2435 2582 2720 2798 2822 5702
Size: 1079 49 68 147 138 78 24 2880
Cuts at: 5702 1239
Size: 2579
Fragments arranged by size:
2880 2579 1079 147 138 78 68 49
24
Bpu10I CC'TnA_GC
Cuts at: 2397 2397
Size: 7042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 5167 6032 6330 2549
Size: 2618 865 298 3261
Fragments arranged by size:
3261 2618 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2897 5705 882
Size: 617 1398 2808 2219
Fragments arranged by size:
2808 2219 1398 617
BsaAI yAC'GTr
Cuts at: 493 1204 4339 4833 493
Size: 711 3135 494 2702
Fragments arranged by size:
3135 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7036 7036
Size: 7042
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4849 5231 5866 275
Size: 53 83 186 936 3316 382 635 1451
Fragments arranged by size:
3316 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5475 5873 6147 6294 6705 5475
Size: 398 274 147 411 5812
Fragments arranged by size:
5812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2023 2053 3708 3738 3834
Size: 30 165 30 620 30 1655 30 96
Cuts at: 3834 3864 1178
Size: 30 4356
Fragments arranged by size:
4356 1655 620 165 96 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 3775 1186
Size: 1228 1361 4453
Fragments arranged by size:
4453 1361 1228
BseYI C'CCAG_C
Cuts at: 6245 6741 6245
Size: 496 6546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 2204 3653 1163
Size: 573 346 122 1449 4552
Fragments arranged by size:
4552 1449 573 346 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4492 5253 6281 1540
Size: 275 186 2491 761 1028 2301
Fragments arranged by size:
2491 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2528 2541 2550 2736 3215 5103 5188 6259 2528
Size: 13 9 186 479 1888 85 1071 3311
Fragments arranged by size:
3311 1888 1071 479 186 85 13 9
BsmI GAATG_Cn'
Cuts at: 4553 4646 4553
Size: 93 6949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2528 2541 2550 2736 3215 3867 4361
Size: 399 738 13 9 186 479 652 494
Cuts at: 4361 5103 5188 6259 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 738 652 494 479 399
186 85 13 9
BspEI T'CCGG_A
Cuts at: 6705 6705
Size: 7042
BspHI T'CATG_A
Cuts at: 4931 6661 4931
Size: 1730 5312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3962 4397 844
Size: 3118 435 3489
Fragments arranged by size:
3489 3118 435
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 4929 1394
Size: 429 1609 1497 3507
Fragments arranged by size:
3507 1609 1497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3747 3865 5537 5711 65
Size: 1183 2499 118 1672 174 1396
Fragments arranged by size:
2499 1672 1396 1183 174 118
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 3182 5686 5873 1700
Size: 110 268 1104 2504 187 2869
Fragments arranged by size:
2869 2504 1104 268 187 110
BsrGI T'GTAC_A
Cuts at: 96 3279 3474 3724 96
Size: 3183 195 250 3414
Fragments arranged by size:
3414 3183 250 195
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7042
BssSI C'ACGA_G
Cuts at: 5102 6114 5102
Size: 1012 6030
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2178 794
Size: 1384 5658
BstBI TT'CG_AA
Cuts at: 2007 5846 2007
Size: 3839 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7042
BstXI CCAn_nnnn'nTGG
Cuts at: 2094 2704 3718 2094
Size: 610 1014 5418
Fragments arranged by size:
5418 1014 610
BstYI r'GATC_y
Cuts at: 1972 3243 4179 4372 4739 5124 5141 6593 6679
Size: 1271 936 193 367 385 17 1452 86
Cuts at: 6679 6691 7037 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1271 936 385 367 346 193
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2893 2981 3093 513
Size: 552 1828 88 112 4462
Fragments arranged by size:
4462 1828 552 112 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6303
Fragments arranged by size:
6303 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 4137 4558 5352 5372 2424
Size: 1713 421 794 20 4094
Fragments arranged by size:
4094 1713 794 421 20
ClaI AT'CG_AT
Cuts at: 1554 4324 1554
Size: 2770 4272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2118 2153 529
Size: 35 1554 35 5418
Fragments arranged by size:
5418 1554 35 35
DraI TTT'AAA
Cuts at: 2748 4346 2748
Size: 1598 5444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6049 809
Size: 507 4733 1802
Fragments arranged by size:
4733 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2106 2325
Size: 54 1425 50 275 18 205 71 219
Cuts at: 2325 2955 3090 3433 3652 4158 4489 5378 7007
Size: 630 135 343 219 506 331 889 1629
Cuts at: 7007 8
Size: 43
Fragments arranged by size:
1629 1425 889 630 506 343 331 275
219 219 205 135 71 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4489 1537
Size: 275 2677 4090
Fragments arranged by size:
4090 2677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2655 3401 4972 1353
Size: 1302 746 1571 3423
Fragments arranged by size:
3423 1571 1302 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5629 6003 6149 387
Size: 1064 138 4040 374 146 1280
Fragments arranged by size:
4040 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2734 2734
Size: 7042
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2367 2435 2582 2636 2720 2798
Size: 393 686 49 68 147 54 84 78
Cuts at: 2798 2822 3047 3554 4254 5105 5702 6489 6757
Size: 24 225 507 700 851 597 787 268
Cuts at: 6757 1239
Size: 1524
Fragments arranged by size:
1524 851 787 700 686 597 507 393
268 225 147 84 78 68 54 49
24
EcoO109I rG'GnC_Cy
Cuts at: 2884 2911 3833 3974 2884
Size: 27 922 141 5952
Fragments arranged by size:
5952 922 141 27
EcoRI G'AATT_C
Cuts at: 4064 4351 4729 4064
Size: 287 378 6377
Fragments arranged by size:
6377 378 287
FspI TGC'GCA
Cuts at: 2756 5548 2756
Size: 2792 4250
HaeII r_GCGC'y
Cuts at: 1678 6189 1678
Size: 4511 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5683 5715 5757 5789 1373
Size: 32 135 32 4111 32 42 32 2626
Fragments arranged by size:
4111 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2620 2682 2788 4386 4632 669
Size: 988 281 682 62 106 1598 246 3079
Fragments arranged by size:
3079 1598 988 682 281 246 106 62
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7042
HpaI GTT'AAC
Cuts at: 2682 4632 2682
Size: 1950 5092
KpnI G_GTAC'C
Cuts at: 3537 4367 3537
Size: 830 6212
MfeI C'AATT_G
Cuts at: 4641 4641
Size: 7042
MluI A'CGCG_T
Cuts at: 6918 6918
Size: 7042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4142 6156 6340 1305
Size: 2837 2014 184 2007
Fragments arranged by size:
2837 2014 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 3092 3654 10
Size: 54 1425 343 276 984 562 3398
Fragments arranged by size:
3398 1425 984 562 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2031 2092 2429 3716 3732 4154 5000
Size: 648 865 61 337 1287 16 422 846
Cuts at: 5000 5359 5518 518
Size: 359 159 2042
Fragments arranged by size:
2042 1287 865 846 648 422 359 337
159 61 16
MspA1I CmG'CkG
Cuts at: 1606 2608 2824 2983 2998 3377 3700 3706 3989
Size: 1002 216 159 15 379 323 6 283
Cuts at: 3989 4458 5135 6283 6528 1606
Size: 469 677 1148 245 2120
Fragments arranged by size:
2120 1148 1002 677 469 379 323 283
245 216 159 15 6
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 7042
NcoI C'CATG_G
Cuts at: 513 1065 2893 513
Size: 552 1828 4662
Fragments arranged by size:
4662 1828 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7042
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 7042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7042
NotI GC'GGCC_GC
Cuts at: 4489 4489
Size: 7042
NspI r_CATG'y
Cuts at: 1132 1229 3475 4400 5945 1132
Size: 97 2246 925 1545 2229
Fragments arranged by size:
2246 2229 1545 925 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7042
PciI A'CATG_T
Cuts at: 5941 5941
Size: 7042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4244 4258 1108
Size: 3136 14 3892
Fragments arranged by size:
3892 3136 14
PfoI T'CCnGG_A
Cuts at: 2412 3246 2412
Size: 834 6208
PmeI GTTT'AAAC
Cuts at: 4346 4346
Size: 7042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 6624 6656 5111
Size: 32 1481 32 5497
Fragments arranged by size:
5497 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 3833 3974 2911
Size: 922 141 5979
Fragments arranged by size:
5979 922 141
PshAI GACnn'nnGTC
Cuts at: 1613 2909 1613
Size: 1296 5746
PsiI TTA'TAA
Cuts at: 4612 4612
Size: 7042
PstI C_TGCA'G
Cuts at: 830 2247 2376 2867 3705 4394 830
Size: 1417 129 491 838 689 3478
Fragments arranged by size:
3478 1417 838 689 491 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7042
PvuII CAG'CTG
Cuts at: 2608 2824 2998 3700 3989 2608
Size: 216 174 702 289 5661
Fragments arranged by size:
5661 702 289 216 174
SacI G_AGCT'C
Cuts at: 2736 2736
Size: 7042
SacII CC_GC'GG
Cuts at: 2984 2984
Size: 7042
SalI G'TCGA_C
Cuts at: 1936 4384 1936
Size: 2448 4594
SanDI GG'GwC_CC
Cuts at: 3833 3833
Size: 7042
SapI GCTCTTCn'nnn_
Cuts at: 2655 2655
Size: 7042
SbfI CC_TGCA'GG
Cuts at: 4394 4394
Size: 7042
ScaI AGT'ACT
Cuts at: 5290 7002 5290
Size: 1712 5330
SexAI A'CCwGG_T
Cuts at: 3932 3932
Size: 7042
SfcI C'TryA_G
Cuts at: 826 1045 2243 2372 2863 3701 4390 5525 6206
Size: 219 1198 129 491 838 689 1135 681
Cuts at: 6206 6397 826
Size: 191 1471
Fragments arranged by size:
1471 1198 1135 838 689 681 491 219
191 129
SfiI GGCCn_nnn'nGGCC
Cuts at: 4254 4254
Size: 7042
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7042
SmaI CCC'GGG
Cuts at: 1872 3426 4369 1872
Size: 1554 943 4545
Fragments arranged by size:
4545 1554 943
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2564 5146 6047 6309 820
Size: 197 926 621 2582 901 262 1553
Fragments arranged by size:
2582 1553 926 901 621 262 197
SnaBI TAC'GTA
Cuts at: 493 4339 4833 493
Size: 3846 494 2702
Fragments arranged by size:
3846 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7042
SphI G_CATG'C
Cuts at: 1132 4400 1132
Size: 3268 3774
SspI AAT'ATT
Cuts at: 5 52 4966 5
Size: 47 4914 2081
Fragments arranged by size:
4914 2081 47
StuI AGG'CCT
Cuts at: 1517 2591 4257 1517
Size: 1074 1666 4302
Fragments arranged by size:
4302 1666 1074
StyI C'CwwG_G
Cuts at: 513 1065 2202 2893 3153 4252 4481 513
Size: 552 1137 691 260 1099 229 3074
Fragments arranged by size:
3074 1137 1099 691 552 260 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2921 5069 5086 5239 5424 5869 325
Size: 1255 1341 2148 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1341 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 3474 3724
Size: 274 80 33 51 1443 1302 195 250
Cuts at: 3724 5288 7000 96
Size: 1564 1712 138
Fragments arranged by size:
1712 1564 1443 1302 274 250 195 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 3834 3866 7002 7034 2095
Size: 32 1707 32 3136 32 2103
Fragments arranged by size:
3136 2103 1707 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7042
XbaI T'CTAG_A
Cuts at: 4378 4378
Size: 7042
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4190 4190
Size: 7042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7042
XmaI C'CCGG_G
Cuts at: 1870 3424 4367 1870
Size: 1554 943 4545
Fragments arranged by size:
4545 1554 943
XmnI GAAnn'nnTTC
Cuts at: 2342 2670 5171 2342
Size: 328 2501 4213
Fragments arranged by size:
4213 2501 328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4850 276
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SfoI SrfI
SwaI