Restriction map for
pFN21ASDA0479
(Circular) (Six-base) MAPSORT of: pFN21ASDA0479.seq Check: 8886 from: 1 to: 5650
pFN21ASDA0479 5650 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 5097 5365 1632
Size: 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2594 1184
Size: 1410 4240
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2248 2959 3185 3337 5446 1493
Size: 755 711 226 152 2109 1697
Fragments arranged by size:
2109 1697 755 711 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2715 2975 1870
Size: 73 772 260 4545
Fragments arranged by size:
4545 772 260 73
BamHI G'GATC_C
Cuts at: 2604 2980 3347 2604
Size: 376 367 4907
Fragments arranged by size:
4907 376 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2214 2710 2971 4426 618
Size: 325 217 627 427 496 261 1455 1842
Fragments arranged by size:
1842 1455 627 496 427 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2658 2969 1391
Size: 1267 311 4072
Fragments arranged by size:
4072 1267 311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BbvCI CC'TCA_GC
Cuts at: 2501 2501
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2475 5645 2475
Size: 3170 2480
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2573 2715 3711 4867 1790
Size: 304 479 142 996 1156 2573
Fragments arranged by size:
2573 1156 996 479 304 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 2516
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 4310 1239
Size: 1415 1656 2579
Fragments arranged by size:
2579 1656 1415
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 2411 4313 882
Size: 617 525 387 1902 2219
Fragments arranged by size:
2219 1902 617 525 387
BsaAI yAC'GTr
Cuts at: 493 1204 2947 3441 493
Size: 711 1743 494 2702
Fragments arranged by size:
2702 1743 711 494
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2743 1186
Size: 1557 4093
BseYI C'CCAG_C
Cuts at: 2424 2889 4853 5349 2424
Size: 465 1964 496 2725
Fragments arranged by size:
2725 1964 496 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 1163
Size: 573 375 4702
Fragments arranged by size:
4702 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 2563 3161 3254 2563
Size: 598 93 4959
Fragments arranged by size:
4959 598 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2225 2225
Size: 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2573 2658 2715 2969 3711 3796
Size: 399 304 479 85 57 254 742 85
Cuts at: 3796 4867 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 479 399 304 254 85
85 57
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2337 2742 3539 5269 2337
Size: 405 797 1730 2718
Fragments arranged by size:
2718 1730 797 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 2637 3005 844
Size: 1471 322 368 3489
Fragments arranged by size:
3489 1471 368 322
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2211 2527 2893 4294 4481 1700
Size: 110 401 316 366 1401 187 2869
Fragments arranged by size:
2869 1401 401 366 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 2925 96
Size: 2829 2821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 2201 3710 4722 2201
Size: 1509 1012 3129
Fragments arranged by size:
3129 1509 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 2007 4454 2007
Size: 2447 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2658 2479
Size: 179 5471
BstYI r'GATC_y
Cuts at: 1972 2475 2508 2604 2980 3347 3732 3749 5201
Size: 503 33 96 376 367 385 17 1452
Cuts at: 5201 5287 5299 5645 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 503 385 376 367 346 96
86 33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5098
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 3166 3960 3980 2463
Size: 703 794 20 4133
Fragments arranged by size:
4133 794 703 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2451 2866
Size: 54 1425 50 275 18 408 213 415
Cuts at: 2866 3097 3986 5615 8
Size: 231 889 1629 43
Fragments arranged by size:
1629 1425 889 415 408 275 231 213
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 2583 3580 1353
Size: 983 247 997 3423
Fragments arranged by size:
3423 997 983 247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 4237 4611 4757 387
Size: 1064 138 921 1727 374 146 1280
Fragments arranged by size:
1727 1280 1064 921 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2654 3713 4310 5097 5365 1239
Size: 393 1022 1059 597 787 268 1524
Fragments arranged by size:
1524 1059 1022 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2197 2332 2197
Size: 135 5515
EcoRI G'AATT_C
Cuts at: 2248 2959 3337 2248
Size: 711 378 4561
Fragments arranged by size:
4561 711 378
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 4291 4323 4365
Size: 32 135 32 1298 32 1389 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 1389 1298 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 2994 3240 669
Size: 988 281 436 620 246 3079
Fragments arranged by size:
3079 988 620 436 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 4764 4948 1305
Size: 993 2466 184 2007
Fragments arranged by size:
2466 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 10
Size: 54 1425 343 621 3207
Fragments arranged by size:
3207 1425 621 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2576 2786 2789 3608 3967 4126
Size: 648 933 477 210 3 819 359 159
Cuts at: 4126 518
Size: 2042
Fragments arranged by size:
2042 933 819 648 477 359 210 159
3
MspA1I CmG'CkG
Cuts at: 1606 2236 2923 3066 3743 4891 5136 1606
Size: 630 687 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 630 245 143
NaeI GCC'GGC
Cuts at: 2213 2213
Size: 5650
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NgoMIV G'CCGG_C
Cuts at: 2211 2211
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2651 1348
Size: 1303 4347
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2875 1108
Size: 1767 3883
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2332 2332
Size: 5650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2312 2470 2856 3002 830
Size: 1482 158 386 146 3478
Fragments arranged by size:
3478 1482 386 158 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2236 2923 2236
Size: 687 4963
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2308 2466 2852 2998 4133 4814 5005
Size: 219 1263 158 386 146 1135 681 191
Cuts at: 5005 826
Size: 1471
Fragments arranged by size:
1471 1263 1135 681 386 219 191 158
146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 2527 1700
Size: 827 4823
SmaI CCC'GGG
Cuts at: 1872 2717 2977 1872
Size: 845 260 4545
Fragments arranged by size:
4545 845 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2947 3441 493
Size: 2454 494 2702
Fragments arranged by size:
2702 2454 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2273 3089 513
Size: 552 1208 816 3074
Fragments arranged by size:
3074 1208 816 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2751 3677 3694 3847 4032 4477
Size: 1255 462 709 926 17 153 185 445
Cuts at: 4477 325
Size: 1498
Fragments arranged by size:
1498 1255 926 709 462 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2925 3896 5608
Size: 274 80 33 51 1443 948 971 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1443 971 948 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2500 2532 5610 5642 2500
Size: 32 3078 32 2508
Fragments arranged by size:
3078 2508 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2842 2183
Size: 659 4991
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2715 2975 1870
Size: 845 260 4545
Fragments arranged by size:
4545 845 260
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI