Restriction map for
pFN21ASDA0478
(Circular) (Six-base) MAPSORT of: pFN21ASDA0478.seq Check: 7590 from: 1 to: 8446
pFN21ASDA0478 8446 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4385 5536 5575 5614 4385
Size: 1151 39 39 7217
Fragments arranged by size:
7217 1151 39 39
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6256 278
Size: 53 83 186 936 4720 2468
Fragments arranged by size:
4720 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2320 3027 5789 1937
Size: 383 707 2762 4594
Fragments arranged by size:
4594 2762 707 383
Acc65I G'GTAC_C
Cuts at: 2114 5088 5767 2114
Size: 2974 679 4793
Fragments arranged by size:
4793 2974 679
AclI AA'CG_TT
Cuts at: 1563 6573 6946 1563
Size: 5010 373 3063
Fragments arranged by size:
5010 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2496 2673 3279 3909 4182 4595 4892
Size: 478 386 177 606 630 273 413 297
Cuts at: 4892 4966 5023 6509 7893 8161 1632
Size: 74 57 1486 1384 268 1917
Fragments arranged by size:
1917 1486 1384 630 606 478 413 386
297 273 268 177 74 57
AfeI AGC'GCT
Cuts at: 4292 4292
Size: 8446
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8249
AflIII A'CryG_T
Cuts at: 1203 4445 4967 7345 8322 1203
Size: 3242 522 2378 977 1327
Fragments arranged by size:
3242 2378 1327 977 522
AgeI A'CCGG_T
Cuts at: 7277 7277
Size: 8446
AhdI GACnn_n'nnGTC
Cuts at: 7175 7175
Size: 8446
AleI CACnn'nnGTG
Cuts at: 1166 5161 1166
Size: 3995 4451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4334 1184
Size: 3150 5296
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 4595 4627 2072
Size: 32 2491 32 5891
Fragments arranged by size:
5891 2491 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2053 2062 3845 4214 4797 5172 6189 7761 2053
Size: 9 1783 369 583 375 1017 1572 2738
Fragments arranged by size:
2738 1783 1572 1017 583 375 369 9
ApaI G_GGCC'C
Cuts at: 2531 2531
Size: 8446
ApaLI G'TGCA_C
Cuts at: 4280 4937 6503 7659 4280
Size: 657 1566 1156 5067
Fragments arranged by size:
5067 1566 1156 657
ApoI r'AATT_y
Cuts at: 1493 2587 2746 3164 3238 4076 4466 5755 5981
Size: 1094 159 418 74 838 390 1289 226
Cuts at: 5981 6133 8242 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1289 1094 838 418 390 226
159 152 74
AseI AT'TA_AT
Cuts at: 160 1032 7000 160
Size: 872 5968 1606
Fragments arranged by size:
5968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8446
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 5771 1870
Size: 73 282 3546 4545
Fragments arranged by size:
4545 3546 282 73
BamHI G'GATC_C
Cuts at: 5776 6143 5776
Size: 367 8079
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 3698 5088 5767 7222
Size: 325 217 627 327 1584 1390 679 1455
Cuts at: 7222 618
Size: 1842
Fragments arranged by size:
1842 1584 1455 1390 679 627 327 325
217
BanII G_rGCy'C
Cuts at: 1391 2031 2134 2225 2531 4407 5271 5439 5568
Size: 640 103 91 306 1876 864 168 129
Cuts at: 5568 5676 5765 1391
Size: 108 89 4072
Fragments arranged by size:
4072 1876 864 640 306 168 129 108
103 91 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2708 2764 3272 3317 4131 928
Size: 971 809 56 508 45 814 5243
Fragments arranged by size:
5243 971 814 809 508 56 45
BbvCI CC'TCA_GC
Cuts at: 3279 3279
Size: 8446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2216 2250 6637 6671 1903
Size: 34 279 34 4387 34 3678
Fragments arranged by size:
4387 3678 279 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6338 7548 6338
Size: 1210 7236
BclI T'GATC_A
Cuts at: 1548 4150 5589 1548
Size: 2602 1439 4405
Fragments arranged by size:
4405 2602 1439
BfrBI ATG'CAT
Cuts at: 2210 4703 2210
Size: 2493 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7057 136
Size: 107 122 71 631 605 5385 1525
Fragments arranged by size:
5385 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4977 8441 4977
Size: 3464 4982
BlpI GC'TnA_GC
Cuts at: 3911 5863 3911
Size: 1952 6494
Bme1580I G_kGCm'C
Cuts at: 1790 2531 4284 4941 4990 6507 7663 1790
Size: 741 1753 657 49 1517 1156 2573
Fragments arranged by size:
2573 1753 1517 1156 741 657 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4726 6177 7135
Size: 416 53 83 399 24 3309 1451 958
Cuts at: 7135 442
Size: 1753
Fragments arranged by size:
3309 1753 1451 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4957 4957
Size: 8446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2505 3284 3528 4400 5021 5144 5391 7106
Size: 1266 779 244 872 621 123 247 1715
Cuts at: 7106 1239
Size: 2579
Fragments arranged by size:
2579 1715 1266 872 779 621 247 244
123
Bpu10I CC'TnA_GC
Cuts at: 2308 3279 2308
Size: 971 7475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2439 6571 7436 7734 2439
Size: 4132 865 298 3151
Fragments arranged by size:
4132 3151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3121 4334 5411 5597 7109 882
Size: 617 1622 1213 1077 186 1512 2219
Fragments arranged by size:
2219 1622 1512 1213 1077 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 4756 4968 5743 6237 493
Size: 711 3552 212 775 494 2702
Fragments arranged by size:
3552 2702 775 711 494 212
BsaBI GATnn'nnATC
Cuts at: 8440 8440
Size: 8446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6253 6635 7270 275
Size: 53 83 186 936 4720 382 635 1451
Fragments arranged by size:
4720 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4917 5334 6879 7277 7551 7698 8109 4917
Size: 417 1545 398 274 147 411 5254
Fragments arranged by size:
5254 1545 417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 2276 2306 1178
Size: 30 165 30 669 30 174 30 7318
Fragments arranged by size:
7318 669 174 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 3071 3287 3839 3842 5615 1186
Size: 1057 828 216 552 3 1773 4017
Fragments arranged by size:
4017 1773 1057 828 552 216 3
BseYI C'CCAG_C
Cuts at: 2629 5325 5466 5607 7649 8145 2629
Size: 2696 141 141 2042 496 2930
Fragments arranged by size:
2930 2696 2042 496 141 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3056 3985 4340 4354 4413 4826 4906
Size: 573 1320 929 355 14 59 413 80
Cuts at: 4906 5185 1163
Size: 279 4424
Fragments arranged by size:
4424 1320 929 573 413 355 279 80
59 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5896 6657 7685 1540
Size: 275 186 3895 761 1028 2301
Fragments arranged by size:
3895 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3053 3834 4284 4512 4941 5205 5271 5403
Size: 1022 781 450 228 429 264 66 132
Cuts at: 5403 5439 5496 5513 5676 6507 6592 7663 2031
Size: 36 57 17 163 831 85 1071 2814
Fragments arranged by size:
2814 1071 1022 831 781 450 429 264
228 163 132 85 66 57 36 17
BsmI GAATG_Cn'
Cuts at: 2623 2894 3864 5314 5957 6050 2623
Size: 271 970 1450 643 93 5019
Fragments arranged by size:
5019 1450 970 643 271 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3415 3415
Size: 8446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2134 2225 2531 3053 3834 4284
Size: 399 241 103 91 306 522 781 450
Cuts at: 4284 4407 4512 4941 4990 5205 5271 5403 5439
Size: 123 105 429 49 215 66 132 36
Cuts at: 5439 5496 5513 5568 5676 5765 6507 6592 7663
Size: 57 17 55 108 89 742 85 1071
Cuts at: 7663 1391
Size: 2174
Fragments arranged by size:
2174 1071 781 742 522 450 429 399
306 241 215 132 123 108 105 103
91 89 85 66 57 55 49 36
17
BspEI T'CCGG_A
Cuts at: 8109 8109
Size: 8446
BspHI T'CATG_A
Cuts at: 3430 4632 6335 8065 3430
Size: 1202 1703 1730 3811
Fragments arranged by size:
3811 1730 1703 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3675 4385 5284 5536 5575 5614 5801 844
Size: 2831 710 899 252 39 39 187 3489
Fragments arranged by size:
3489 2831 899 710 252 187 39 39
BsrBI CCG'CTC
Cuts at: 1394 1823 5005 6333 1394
Size: 429 3182 1328 3507
Fragments arranged by size:
3507 3182 1328 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5375 6941 7115 65
Size: 1183 4127 1566 174 1396
Fragments arranged by size:
4127 1566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 7090 7277 1700
Size: 110 233 5047 187 2869
Fragments arranged by size:
5047 2869 233 187 110
BsrGI T'GTAC_A
Cuts at: 96 2064 2256 96
Size: 1968 192 6286
Fragments arranged by size:
6286 1968 192
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8446
BssSI C'ACGA_G
Cuts at: 5675 6506 7518 5675
Size: 831 1012 6603
Fragments arranged by size:
6603 1012 831
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 3628 4626 4884 5228 794
Size: 1259 1575 998 258 344 4012
Fragments arranged by size:
4012 1575 1259 998 344 258
BstBI TT'CG_AA
Cuts at: 2007 7250 2007
Size: 5243 3203
BstEII G'GTnAC_C
Cuts at: 1184 2791 5014 1184
Size: 1607 2223 4616
Fragments arranged by size:
4616 2223 1607
BstYI r'GATC_y
Cuts at: 1972 4864 4977 5049 5776 6143 6528 6545 7997
Size: 2892 113 72 727 367 385 17 1452
Cuts at: 7997 8083 8095 8441 1972
Size: 86 12 346 1977
Fragments arranged by size:
2892 1977 1452 727 385 367 346 113
86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 3758 4480 3758
Size: 722 7724
BtgI C'CryG_G
Cuts at: 513 1065 2022 3921 4387 5394 5568 513
Size: 552 957 1899 466 1007 174 3391
Fragments arranged by size:
3391 1899 1007 957 552 466 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7707
Fragments arranged by size:
7707 683 56
BtsI GCAGTG_nn'
Cuts at: 3726 3912 3973 4328 4342 4624 5962 6756 6776
Size: 186 61 355 14 282 1338 794 20
Cuts at: 6776 3726
Size: 5396
Fragments arranged by size:
5396 1338 794 355 282 186 61 20
14
ClaI AT'CG_AT
Cuts at: 1554 2205 1554
Size: 651 7795
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8411
DraI TTT'AAA
Cuts at: 2808 5750 2808
Size: 2942 5504
DraIII CAC_nnn'GTG
Cuts at: 2073 5014 2073
Size: 2941 5505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7453 809
Size: 507 6137 1802
Fragments arranged by size:
6137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3939 5893 6782
Size: 54 1425 50 275 18 2109 1954 889
Cuts at: 6782 8411 8
Size: 1629 43
Fragments arranged by size:
2109 1954 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5893 1537
Size: 275 4081 4090
Fragments arranged by size:
4090 4081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3255 4217 4424 4533 4911 5276 5456 6376
Size: 1902 962 207 109 378 365 180 920
Cuts at: 6376 1353
Size: 3423
Fragments arranged by size:
3423 1902 962 920 378 365 207 180
109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 7033 7407 7553 387
Size: 1064 138 3053 2391 374 146 1280
Fragments arranged by size:
3053 2391 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2029 5269 5437 5674 2029
Size: 3240 168 237 4801
Fragments arranged by size:
4801 3240 237 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2496 2505 2673 3279 3284 3528
Size: 393 478 386 9 168 606 5 244
Cuts at: 3528 3909 4182 4400 4595 4892 4966 5021 5023
Size: 381 273 218 195 297 74 55 2
Cuts at: 5023 5144 5391 6509 7106 7893 8161 1239
Size: 121 247 1118 597 787 268 1524
Fragments arranged by size:
1524 1118 787 606 597 478 393 386
381 297 273 268 247 244 218 195
168 121 74 55 9 5 2
EcoNI CCTnn'n_nnAGG
Cuts at: 4034 4034
Size: 8446
EcoO109I rG'GnC_Cy
Cuts at: 2527 2528 3100 4761 5322 2527
Size: 1 572 1661 561 5651
Fragments arranged by size:
5651 1661 572 561 1
EcoRI G'AATT_C
Cuts at: 2587 3164 5755 6133 2587
Size: 577 2591 378 4900
Fragments arranged by size:
4900 2591 577 378
EcoRV GAT'ATC
Cuts at: 3576 3576
Size: 8446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 4243 5287 2305
Size: 1938 1044 5464
Fragments arranged by size:
5464 1938 1044
FspI TGC'GCA
Cuts at: 6952 6952
Size: 8446
HaeII r_GCGC'y
Cuts at: 1678 4294 7593 1678
Size: 2616 3299 2531
Fragments arranged by size:
3299 2616 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 3412 3444 7087
Size: 32 135 32 611 32 1197 32 3643
Cuts at: 7087 7119 7161 7193 1373
Size: 32 42 32 2626
Fragments arranged by size:
3643 2626 1197 611 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5790 6036 669
Size: 988 281 3852 246 3079
Fragments arranged by size:
3852 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2138 2655 3530 3891 4252 748
Size: 1390 517 875 361 361 4942
Fragments arranged by size:
4942 1390 875 517 361 361
HpaI GTT'AAC
Cuts at: 6036 6036
Size: 8446
KpnI G_GTAC'C
Cuts at: 2118 5092 5771 2118
Size: 2974 679 4793
Fragments arranged by size:
4793 2974 679
MfeI C'AATT_G
Cuts at: 5072 6045 5072
Size: 973 7473
MluI A'CGCG_T
Cuts at: 8322 8322
Size: 8446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 3409 3814 5005 7560 7744 1305
Size: 921 1183 405 1191 2555 184 2007
Fragments arranged by size:
2555 2007 1191 1183 921 405 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6624
Fragments arranged by size:
6624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4515 4929 5161 5250 5335 6404 6763
Size: 648 3349 414 232 89 85 1069 359
Cuts at: 6763 6922 518
Size: 159 2042
Fragments arranged by size:
3349 2042 1069 648 414 359 232 159
89 85
MspA1I CmG'CkG
Cuts at: 1606 2050 2062 2633 2651 2743 3124 4378 5043
Size: 444 12 571 18 92 381 1254 665
Cuts at: 5043 5862 6539 7687 7932 1606
Size: 819 677 1148 245 2120
Fragments arranged by size:
2120 1254 1148 819 677 665 571 444
381 245 92 18 12
NaeI GCC'GGC
Cuts at: 2045 2045
Size: 8446
NcoI C'CATG_G
Cuts at: 513 1065 2022 3921 4387 513
Size: 552 957 1899 466 4572
Fragments arranged by size:
4572 1899 957 552 466
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8446
NgoMIV G'CCGG_C
Cuts at: 2043 2043
Size: 8446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8446
NotI GC'GGCC_GC
Cuts at: 5893 5893
Size: 8446
NsiI A_TGCA'T
Cuts at: 2212 4705 2212
Size: 2493 5953
NspI r_CATG'y
Cuts at: 1132 1229 3740 4449 4489 4707 5231 5804 7349
Size: 97 2511 709 40 218 524 573 1545
Cuts at: 7349 1132
Size: 2229
Fragments arranged by size:
2511 2229 1545 709 573 524 218 97
40
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8446
PciI A'CATG_T
Cuts at: 4445 7345 4445
Size: 2900 5546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8446
PfoI T'CCnGG_A
Cuts at: 5368 5368
Size: 8446
PmeI GTTT'AAAC
Cuts at: 5750 5750
Size: 8446
PmlI CAC'GTG
Cuts at: 4756 4968 4756
Size: 212 8234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2072 2104 6515 6547 8028 8060 2072
Size: 32 4411 32 1481 32 2458
Fragments arranged by size:
4411 2458 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3100 4761 5322 3100
Size: 1661 561 6224
Fragments arranged by size:
6224 1661 561
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8446
PsiI TTA'TAA
Cuts at: 6016 6016
Size: 8446
PspOMI G'GGCC_C
Cuts at: 2527 2527
Size: 8446
PstI C_TGCA'G
Cuts at: 830 2058 2989 3996 4793 5295 5798 830
Size: 1228 931 1007 797 502 503 3478
Fragments arranged by size:
3478 1228 1007 931 797 503 502
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8446
PvuII CAG'CTG
Cuts at: 2050 2062 2651 2743 3124 4378 5043 2050
Size: 12 589 92 381 1254 665 5453
Fragments arranged by size:
5453 1254 665 589 381 92 12
SacI G_AGCT'C
Cuts at: 2031 5271 5439 5676 2031
Size: 3240 168 237 4801
Fragments arranged by size:
4801 3240 237 168
SalI G'TCGA_C
Cuts at: 1936 5788 1936
Size: 3852 4594
SanDI GG'GwC_CC
Cuts at: 5322 5322
Size: 8446
SapI GCTCTTCn'nnn_
Cuts at: 4217 5276 4217
Size: 1059 7387
SbfI CC_TGCA'GG
Cuts at: 5798 5798
Size: 8446
ScaI AGT'ACT
Cuts at: 4712 6694 8406 4712
Size: 1982 1712 4752
Fragments arranged by size:
4752 1982 1712
SfcI C'TryA_G
Cuts at: 826 1045 2038 2054 2321 2646 2985 3992 4789
Size: 219 993 16 267 325 339 1007 797
Cuts at: 4789 5291 5794 6929 7610 7801 826
Size: 502 503 1135 681 191 1471
Fragments arranged by size:
1471 1135 1007 993 797 681 503 502
339 325 267 219 191 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8446
SmaI CCC'GGG
Cuts at: 1872 5773 1872
Size: 3901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2454 6550 7451 7713 820
Size: 197 926 511 4096 901 262 1553
Fragments arranged by size:
4096 1553 926 901 511 262 197
SnaBI TAC'GTA
Cuts at: 493 5743 6237 493
Size: 5250 494 2702
Fragments arranged by size:
5250 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8446
SphI G_CATG'C
Cuts at: 1132 4489 4707 5804 1132
Size: 3357 218 1097 3774
Fragments arranged by size:
3774 3357 1097 218
SspI AAT'ATT
Cuts at: 5 52 2975 6370 5
Size: 47 2923 3395 2081
Fragments arranged by size:
3395 2923 2081 47
StuI AGG'CCT
Cuts at: 1517 3990 4241 4649 5063 1517
Size: 2473 251 408 414 4900
Fragments arranged by size:
4900 2473 414 408 251
StyI C'CwwG_G
Cuts at: 513 1065 2022 3156 3222 3921 4344 4387 5885
Size: 552 957 1134 66 699 423 43 1498
Cuts at: 5885 513
Size: 3074
Fragments arranged by size:
3074 1498 1134 957 699 552 423 66
43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3235 4658 5511 6473 6490 6643 6828
Size: 1255 1655 1423 853 962 17 153 185
Cuts at: 6828 7273 325
Size: 445 1498
Fragments arranged by size:
1655 1498 1423 1255 962 853 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2064 2256 4710
Size: 274 80 33 51 1443 87 192 2454
Cuts at: 4710 5343 6692 8404 96
Size: 633 1349 1712 138
Fragments arranged by size:
2454 1712 1443 1349 633 274 192 138
87 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 5126 5158 8406 8438 2276
Size: 32 2818 32 3248 32 2284
Fragments arranged by size:
3248 2818 2284 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3070 1316
Size: 1754 6692
XbaI T'CTAG_A
Cuts at: 2755 3586 5782 2755
Size: 831 2196 5419
Fragments arranged by size:
5419 2196 831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4394 5075 4394
Size: 681 7765
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8446
XmaI C'CCGG_G
Cuts at: 1870 5771 1870
Size: 3901 4545
XmnI GAAnn'nnTTC
Cuts at: 2867 4226 4526 4979 5036 6575 2867
Size: 1359 300 453 57 1539 4738
Fragments arranged by size:
4738 1539 1359 453 300 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6254 276
Size: 53 83 186 936 4720 2468
Fragments arranged by size:
4720 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I FseI
FspAI KasI NarI NruI PacI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI