Restriction map for
pFN21ASDA0475
(Circular) (Six-base) MAPSORT of: pFN21ASDA0475.seq Check: 1066 from: 1 to: 5956
pFN21ASDA0475 5956 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 4019 5403 5671 1632
Size: 645 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 645 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaLI G'TGCA_C
Cuts at: 3187 4013 5169 3187
Size: 826 1156 3974
Fragments arranged by size:
3974 1156 826
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 3281 1870
Size: 73 298 1040 4545
Fragments arranged by size:
4545 1040 298 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 3277 4732 618
Size: 325 217 627 392 1098 1455 1842
Fragments arranged by size:
1842 1455 1098 627 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2509 3275 1391
Size: 1118 766 4072
Fragments arranged by size:
4072 1118 766
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3231 928
Size: 971 1332 3653
Fragments arranged by size:
3653 1332 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BfrBI ATG'CAT
Cuts at: 2287 2287
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 3191 4017 5173 1790
Size: 1401 826 1156 2573
Fragments arranged by size:
2573 1401 1156 826
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2221 3110 3687
Size: 416 53 83 399 24 804 889 577
Cuts at: 3687 4645 442
Size: 958 1753
Fragments arranged by size:
1753 958 889 804 577 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4616 1239
Size: 3377 2579
Bpu10I CC'TnA_GC
Cuts at: 2394 2394
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 4619 882
Size: 617 904 2216 2219
Fragments arranged by size:
2219 2216 904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3253 3747 493
Size: 711 2049 494 2702
Fragments arranged by size:
2702 2049 711 494
BsaBI GATnn'nnATC
Cuts at: 3132 5950 3132
Size: 2818 3138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3141 3763 4145 4780
Size: 53 83 186 936 1608 622 382 635
Cuts at: 4780 275
Size: 1451
Fragments arranged by size:
1608 1451 936 635 622 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3095 3125 1178
Size: 30 165 30 1692 30 4009
Fragments arranged by size:
4009 1692 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5956
BseYI C'CCAG_C
Cuts at: 2272 5159 5655 2272
Size: 2887 496 2573
Fragments arranged by size:
2887 2573 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2582 3123 3191 4017 4102 5173 2582
Size: 541 68 826 85 1071 3365
Fragments arranged by size:
3365 1071 826 541 85 68
BsmI GAATG_Cn'
Cuts at: 2991 3467 3560 2991
Size: 476 93 5387
Fragments arranged by size:
5387 476 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2509 2582 3123 3191 3275 4017 4102
Size: 399 719 73 541 68 84 742 85
Cuts at: 4102 5173 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 719 541 399 85 84
73 68
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 844
Size: 2467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2887 4451 4625 65
Size: 1183 1639 1564 174 1396
Fragments arranged by size:
1639 1564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 2007 4760 2007
Size: 2753 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2706 2706
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2678 3286 3653 4038 4055 5507 5593 5605
Size: 706 608 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 706 608 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2292 2732 3093 3144 513
Size: 552 1227 440 361 51 3325
Fragments arranged by size:
3325 1227 552 440 361 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3191 3472 4266 4286 3191
Size: 281 794 20 4861
Fragments arranged by size:
4861 794 281 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2858 4963 809
Size: 507 1542 2105 1802
Fragments arranged by size:
2105 1802 1542 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2763 3171
Size: 54 1425 50 275 18 465 468 408
Cuts at: 3171 3403 4292 5921 8
Size: 232 889 1629 43
Fragments arranged by size:
1629 1425 889 468 465 408 275 232
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 3886 1353
Size: 900 1633 3423
Fragments arranged by size:
3423 1633 900
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 4019 4616 5403 5671 1239
Size: 393 645 1742 597 787 268 1524
Fragments arranged by size:
1742 1524 787 645 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2139 2139
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2928 5103 1678
Size: 1250 2175 2531
Fragments arranged by size:
2531 2175 1250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3095 3127 4597 4629 4671
Size: 32 135 32 1523 32 1470 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1523 1470 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2959 748
Size: 2211 3745
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 2765 3173 10
Size: 54 1425 343 465 468 408 2793
Fragments arranged by size:
2793 1425 468 465 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2898 3026 3914 4273 4432 518
Size: 648 1732 128 888 359 159 2042
Fragments arranged by size:
2042 1732 888 648 359 159 128
MspA1I CmG'CkG
Cuts at: 1606 2117 2344 2572 2858 3154 3372 4049 5197
Size: 511 227 228 286 296 218 677 1148
Cuts at: 5197 5442 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 511 296 286 245 228
227 218
NcoI C'CATG_G
Cuts at: 513 1065 2292 2732 3093 513
Size: 552 1227 440 361 3376
Fragments arranged by size:
3376 1227 552 440 361
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NsiI A_TGCA'T
Cuts at: 2289 2289
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2291 3314 4859 1132
Size: 97 1062 1023 1545 2229
Fragments arranged by size:
2229 1545 1062 1023 97
PasI CC'CwG_GG
Cuts at: 1348 2211 1348
Size: 863 5093
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 2419 1108
Size: 1125 186 4645
Fragments arranged by size:
4645 1125 186
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 2331 3526 2331
Size: 1195 4761
PstI C_TGCA'G
Cuts at: 830 2718 3308 830
Size: 1888 590 3478
Fragments arranged by size:
3478 1888 590
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2117 2344 2572 2858 2117
Size: 227 228 286 5215
Fragments arranged by size:
5215 286 228 227
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 3216 4204 5916 3216
Size: 988 1712 3256
Fragments arranged by size:
3256 1712 988
SfcI C'TryA_G
Cuts at: 826 1045 2592 2714 3304 4439 5120 5311 826
Size: 219 1547 122 590 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 681 590 219 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 2243 3283 1872
Size: 371 1040 4545
Fragments arranged by size:
4545 1040 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3253 3747 493
Size: 2760 494 2702
Fragments arranged by size:
2760 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 2291 3314 1132
Size: 1159 1023 3774
Fragments arranged by size:
3774 1159 1023
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2292 2511 2732 3093 3395 513
Size: 552 1227 219 221 361 302 3074
Fragments arranged by size:
3074 1227 552 361 302 221 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 2221 2745 3983 4000 4153 4338
Size: 1255 617 24 524 1238 17 153 185
Cuts at: 4338 4783 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1238 617 524 445 185 153
24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3214 4202 5914
Size: 274 80 33 51 1443 1237 988 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1712 1443 1237 988 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 2241 3281 1870
Size: 371 1040 4545
Fragments arranged by size:
4545 1040 371
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI