Restriction map for
pFN21ASDA0470
(Circular) (Six-base) MAPSORT of: pFN21ASDA0470.seq Check: 8064 from: 1 to: 9109
pFN21ASDA0470 9109 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6919 278
Size: 53 83 186 936 5383 2468
Fragments arranged by size:
5383 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2174 3786 4788 6346 6452 1937
Size: 237 1612 1002 1558 106 4594
Fragments arranged by size:
4594 1612 1558 1002 237 106
Acc65I G'GTAC_C
Cuts at: 6430 6430
Size: 9109
AclI AA'CG_TT
Cuts at: 1563 3472 7236 7609 1563
Size: 1909 3764 373 3063
Fragments arranged by size:
3764 3063 1909 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 2363 2382 2487 2508 2643 3743 4656
Size: 729 2 19 105 21 135 1100 913
Cuts at: 4656 5046 5127 5250 5385 7172 8556 8824 1632
Size: 390 81 123 135 1787 1384 268 1917
Fragments arranged by size:
1917 1787 1384 1100 913 729 390 268
135 135 123 105 81 21 19 2
AfeI AGC'GCT
Cuts at: 5431 5431
Size: 9109
AflII C'TTAA_G
Cuts at: 820 1017 2351 3220 820
Size: 197 1334 869 6709
Fragments arranged by size:
6709 1334 869 197
AflIII A'CryG_T
Cuts at: 1203 8008 8985 1203
Size: 6805 977 1327
Fragments arranged by size:
6805 1327 977
AgeI A'CCGG_T
Cuts at: 7940 7940
Size: 9109
AhdI GACnn_n'nnGTC
Cuts at: 7838 7838
Size: 9109
AleI CACnn'nnGTG
Cuts at: 1166 4721 1166
Size: 3555 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9109
AlwNI CAG_nnn'CTG
Cuts at: 2459 3189 4526 5612 6088 6852 8424 2459
Size: 730 1337 1086 476 764 1572 3144
Fragments arranged by size:
3144 1572 1337 1086 764 730 476
ApaLI G'TGCA_C
Cuts at: 7166 8322 7166
Size: 1156 7953
ApoI r'AATT_y
Cuts at: 1493 2762 2870 3542 4099 4170 4748 5315 6311
Size: 1269 108 672 557 71 578 567 996
Cuts at: 6311 6418 6644 6796 8905 1493
Size: 107 226 152 2109 1697
Fragments arranged by size:
2109 1697 1269 996 672 578 567 557
226 152 108 107 71
AseI AT'TA_AT
Cuts at: 160 1032 7663 160
Size: 872 6631 1606
Fragments arranged by size:
6631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9109
AvaI C'yCGr_G
Cuts at: 1870 1943 5559 6238 6434 1870
Size: 73 3616 679 196 4545
Fragments arranged by size:
4545 3616 679 196 73
BamHI G'GATC_C
Cuts at: 4375 6439 6806 4375
Size: 2064 367 6678
Fragments arranged by size:
6678 2064 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 6430 7885 618
Size: 325 217 627 1217 3426 1455 1842
Fragments arranged by size:
3426 1842 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2118 3181 3376 6280 6428 1391
Size: 727 1063 195 2904 148 4072
Fragments arranged by size:
4072 2904 1063 727 195 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2949 4014 5493 928
Size: 971 1050 1065 1479 4544
Fragments arranged by size:
4544 1479 1065 1050 971
BbvCI CC'TCA_GC
Cuts at: 4164 4903 5383 4164
Size: 739 480 7890
Fragments arranged by size:
7890 739 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7300 7334 1903
Size: 34 5363 34 3678
Fragments arranged by size:
5363 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5122 7001 8211 5122
Size: 1879 1210 6020
Fragments arranged by size:
6020 1879 1210
BclI T'GATC_A
Cuts at: 1548 2932 3520 4855 1548
Size: 1384 588 1335 5802
Fragments arranged by size:
5802 1384 1335 588
BfrBI ATG'CAT
Cuts at: 5704 5704
Size: 9109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7720 136
Size: 107 122 71 631 605 6048 1525
Fragments arranged by size:
6048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3355 5542 5686 9104 3355
Size: 2187 144 3418 3360
Fragments arranged by size:
3418 3360 2187 144
BlpI GC'TnA_GC
Cuts at: 5181 6526 5181
Size: 1345 7764
Bme1580I G_kGCm'C
Cuts at: 1790 7170 8326 1790
Size: 5380 1156 2573
Fragments arranged by size:
5380 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4485 6171 6840
Size: 416 53 83 399 24 3068 1686 669
Cuts at: 6840 7798 442
Size: 958 1753
Fragments arranged by size:
3068 1753 1686 958 669 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3035 3636 1056
Size: 1979 601 6529
Fragments arranged by size:
6529 1979 601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3675 3872 3675
Size: 197 8912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4074 4584 5887 7769 1239
Size: 2835 510 1303 1882 2579
Fragments arranged by size:
2835 2579 1882 1303 510
Bpu10I CC'TnA_GC
Cuts at: 4164 4903 5383 4164
Size: 739 480 7890
Fragments arranged by size:
7890 739 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2955 3230 3713 3841 5802 6040 7234 8099 8397
Size: 275 483 128 1961 238 1194 865 298
Cuts at: 8397 2955
Size: 3667
Fragments arranged by size:
3667 1961 1194 865 483 298 275 238
128
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2901 7772 882
Size: 617 1402 4871 2219
Fragments arranged by size:
4871 2219 1402 617
BsaAI yAC'GTr
Cuts at: 493 1204 4454 6406 6900 493
Size: 711 3250 1952 494 2702
Fragments arranged by size:
3250 2702 1952 711 494
BsaBI GATnn'nnATC
Cuts at: 2301 3312 9103 2301
Size: 1011 5791 2307
Fragments arranged by size:
5791 2307 1011
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3226 6916 7298 7933
Size: 53 83 186 936 1693 3690 382 635
Cuts at: 7933 275
Size: 1451
Fragments arranged by size:
3690 1693 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2242 4966 5469 7542 7940 8214 8361 8772 2242
Size: 2724 503 2073 398 274 147 411 2579
Fragments arranged by size:
2724 2579 2073 503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3986 4016 4116 4146 4624
Size: 30 165 30 2583 30 100 30 478
Cuts at: 4624 4654 1178
Size: 30 5633
Fragments arranged by size:
5633 2583 478 165 100 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3039 4007 4212 1186
Size: 1853 968 205 6083
Fragments arranged by size:
6083 1853 968 205
BseYI C'CCAG_C
Cuts at: 2980 3173 3666 4259 8312 8808 2980
Size: 193 493 593 4053 496 3281
Fragments arranged by size:
4053 3281 593 496 493 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 2677 2865 3534 1163
Size: 573 756 185 188 669 6738
Fragments arranged by size:
6738 756 669 573 188 185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5797 6559 7320 8348 1540
Size: 275 186 3796 762 761 1028 2301
Fragments arranged by size:
3796 2301 1028 762 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 5047 7170 7255 8326 2118
Size: 2929 2123 85 1071 2901
Fragments arranged by size:
2929 2901 2123 1071 85
BsmI GAATG_Cn'
Cuts at: 6325 6620 6713 6325
Size: 295 93 8721
Fragments arranged by size:
8721 295 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3646 3646
Size: 9109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 3181 3376 5047 6280 6428 7170
Size: 399 328 1063 195 1671 1233 148 742
Cuts at: 7170 7255 8326 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1671 1233 1071 1063 742 399 328
195 148 85
BspEI T'CCGG_A
Cuts at: 2242 8772 2242
Size: 6530 2579
BspHI T'CATG_A
Cuts at: 2800 6998 8728 2800
Size: 4198 1730 3181
Fragments arranged by size:
4198 3181 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6464 844
Size: 5620 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6996 1394
Size: 429 5173 3507
Fragments arranged by size:
5173 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5448 5553 7604 7778 65
Size: 1183 4200 105 2051 174 1396
Fragments arranged by size:
4200 2051 1396 1183 174 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 7753 7940 1700
Size: 110 5943 187 2869
Fragments arranged by size:
5943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4918 96
Size: 4822 4287
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9109
BssSI C'ACGA_G
Cuts at: 2079 7169 8181 2079
Size: 5090 1012 3007
Fragments arranged by size:
5090 3007 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2459 2852 5099 6122 794
Size: 1665 393 2247 1023 3781
Fragments arranged by size:
3781 2247 1665 1023 393
BstBI TT'CG_AA
Cuts at: 2007 7913 2007
Size: 5906 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9109
BstXI CCAn_nnnn'nTGG
Cuts at: 2642 4723 2642
Size: 2081 7028
BstYI r'GATC_y
Cuts at: 1972 3355 4375 5542 5686 6439 6806 7191 7208
Size: 1383 1020 1167 144 753 367 385 17
Cuts at: 7208 8660 8746 8758 9104 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1383 1167 1020 753 385 367
346 144 86 17 12
BstZ17I GTA'TAC
Cuts at: 3787 6347 3787
Size: 2560 6549
BtgI C'CryG_G
Cuts at: 513 1065 2514 2536 513
Size: 552 1449 22 7086
Fragments arranged by size:
7086 1449 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8370
Fragments arranged by size:
8370 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 5610 5634 6625 7419 7439 2059
Size: 3551 24 991 794 20 3729
Fragments arranged by size:
3729 3551 991 794 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3847 3882 529
Size: 35 3283 35 5756
Fragments arranged by size:
5756 3283 35 35
DraI TTT'AAA
Cuts at: 4072 5980 6413 4072
Size: 1908 433 6768
Fragments arranged by size:
6768 1908 433
DraIII CAC_nnn'GTG
Cuts at: 4142 4142
Size: 9109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5366 8116 809
Size: 507 4050 2750 1802
Fragments arranged by size:
4050 2750 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6259 6556 7445
Size: 54 1425 50 275 18 4429 297 889
Cuts at: 7445 9074 8
Size: 1629 43
Fragments arranged by size:
4429 1629 1425 889 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6556 1537
Size: 275 4744 4090
Fragments arranged by size:
4744 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2095 3069 3561 3666 5263 5514 5670 6285
Size: 742 974 492 105 1597 251 156 615
Cuts at: 6285 6372 7039 1353
Size: 87 667 3423
Fragments arranged by size:
3423 1597 974 742 667 615 492 251
156 105 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5872 7696 8070 8216 387
Size: 1064 138 4283 1824 374 146 1280
Fragments arranged by size:
4283 1824 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2116 2116
Size: 9109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2363 2382 2487 2508 2643 3743
Size: 393 729 2 19 105 21 135 1100
Cuts at: 3743 4074 4584 4656 5046 5127 5250 5385 5887
Size: 331 510 72 390 81 123 135 502
Cuts at: 5887 7172 7769 8556 8824 1239
Size: 1285 597 787 268 1524
Fragments arranged by size:
1524 1285 1100 787 729 597 510 502
393 390 331 268 135 135 123 105
81 72 21 19 2
EcoNI CCTnn'n_nnAGG
Cuts at: 5142 5260 5869 5142
Size: 118 609 8382
Fragments arranged by size:
8382 609 118
EcoO109I rG'GnC_Cy
Cuts at: 2335 2335
Size: 9109
EcoRI G'AATT_C
Cuts at: 2762 6418 6796 2762
Size: 3656 378 5075
Fragments arranged by size:
5075 3656 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2366 2729 4386 2366
Size: 363 1657 7089
Fragments arranged by size:
7089 1657 363
FspI TGC'GCA
Cuts at: 5039 7615 5039
Size: 2576 6533
HaeII r_GCGC'y
Cuts at: 1678 2941 3190 5433 8256 1678
Size: 1263 249 2243 2823 2531
Fragments arranged by size:
2823 2531 2243 1263 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 4796 4828 4832
Size: 32 135 32 1100 32 2092 32 4
Cuts at: 4832 4864 5078 5110 5371 5403 5497 5529 7750
Size: 32 214 32 261 32 94 32 2221
Cuts at: 7750 7782 7824 7856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2221 2092 1100 261 214 135 94
42 32 32 32 32 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4584 6453 6699 669
Size: 988 281 2646 1869 246 3079
Fragments arranged by size:
3079 2646 1869 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5058 748
Size: 4310 4799
HpaI GTT'AAC
Cuts at: 6699 6699
Size: 9109
KpnI G_GTAC'C
Cuts at: 6434 6434
Size: 9109
MfeI C'AATT_G
Cuts at: 6708 6708
Size: 9109
MluI A'CGCG_T
Cuts at: 8985 8985
Size: 9109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2077 8223 8407 1305
Size: 772 6146 184 2007
Fragments arranged by size:
6146 2007 772 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6261 10
Size: 54 1425 343 4429 2858
Fragments arranged by size:
4429 2858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2653 3144 3599 4721 7067 7426 7585
Size: 648 1487 491 455 1122 2346 359 159
Cuts at: 7585 518
Size: 2042
Fragments arranged by size:
2346 2042 1487 1122 648 491 455 359
159
MspA1I CmG'CkG
Cuts at: 1606 2696 4907 5957 6109 6308 6525 7202 8350
Size: 1090 2211 1050 152 199 217 677 1148
Cuts at: 8350 8595 1606
Size: 245 2120
Fragments arranged by size:
2211 2120 1148 1090 1050 677 245 217
199 152
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 7108
Fragments arranged by size:
7108 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9109
NheI G'CTAG_C
Cuts at: 1052 3031 3632 1052
Size: 1979 601 6529
Fragments arranged by size:
6529 1979 601
NotI GC'GGCC_GC
Cuts at: 6556 6556
Size: 9109
NsiI A_TGCA'T
Cuts at: 5706 5706
Size: 9109
NspI r_CATG'y
Cuts at: 1132 1229 2855 4919 6467 8012 1132
Size: 97 1626 2064 1548 1545 2229
Fragments arranged by size:
2229 2064 1626 1548 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2888 1348
Size: 1540 7569
PciI A'CATG_T
Cuts at: 8008 8008
Size: 9109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2552 6257 1108
Size: 1444 3705 3960
Fragments arranged by size:
3960 3705 1444
PfoI T'CCnGG_A
Cuts at: 2962 3907 2962
Size: 945 8164
PmeI GTTT'AAAC
Cuts at: 6413 6413
Size: 9109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7178 7210 8691 8723 7178
Size: 32 1481 32 7564
Fragments arranged by size:
7564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2335
Size: 9109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9109
PsiI TTA'TAA
Cuts at: 3324 4497 6679 3324
Size: 1173 2182 5754
Fragments arranged by size:
5754 2182 1173
PstI C_TGCA'G
Cuts at: 830 2695 3391 6461 830
Size: 1865 696 3070 3478
Fragments arranged by size:
3478 3070 1865 696
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9109
PvuII CAG'CTG
Cuts at: 2696 4907 5957 6109 6308 2696
Size: 2211 1050 152 199 5497
Fragments arranged by size:
5497 2211 1050 199 152
SacI G_AGCT'C
Cuts at: 2118 2118
Size: 9109
SalI G'TCGA_C
Cuts at: 1936 6451 1936
Size: 4515 4594
SanDI GG'GwC_CC
Cuts at: 2335 2335
Size: 9109
SapI GCTCTTCn'nnn_
Cuts at: 6285 6285
Size: 9109
SbfI CC_TGCA'GG
Cuts at: 6461 6461
Size: 9109
ScaI AGT'ACT
Cuts at: 2712 3610 7357 9069 2712
Size: 898 3747 1712 2752
Fragments arranged by size:
3747 2752 1712 898
SfcI C'TryA_G
Cuts at: 826 1045 2313 2691 2725 3190 3251 3387 4248
Size: 219 1268 378 34 465 61 136 861
Cuts at: 4248 5943 6457 7592 8273 8464 826
Size: 1695 514 1135 681 191 1471
Fragments arranged by size:
1695 1471 1268 1135 861 681 514 465
378 219 191 136 61 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9109
SmaI CCC'GGG
Cuts at: 1872 6240 6436 1872
Size: 4368 196 4545
Fragments arranged by size:
4545 4368 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2970 3209 3220 3692 3820
Size: 197 926 408 619 239 11 472 128
Cuts at: 3820 5817 6055 7213 8114 8376 820
Size: 1997 238 1158 901 262 1553
Fragments arranged by size:
1997 1553 1158 926 901 619 472 408
262 239 238 197 128 11
SnaBI TAC'GTA
Cuts at: 493 6406 6900 493
Size: 5913 494 2702
Fragments arranged by size:
5913 2702 494
SpeI A'CTAG_T
Cuts at: 152 5299 5450 152
Size: 5147 151 3811
Fragments arranged by size:
5147 3811 151
SphI G_CATG'C
Cuts at: 1132 2855 6467 1132
Size: 1723 3612 3774
Fragments arranged by size:
3774 3612 1723
SspI AAT'ATT
Cuts at: 5 52 5973 7033 5
Size: 47 5921 1060 2081
Fragments arranged by size:
5921 2081 1060 47
StuI AGG'CCT
Cuts at: 1517 3331 1517
Size: 1814 7295
StyI C'CwwG_G
Cuts at: 513 1065 2514 3627 5006 5364 6548 513
Size: 552 1449 1113 1379 358 1184 3074
Fragments arranged by size:
3074 1449 1379 1184 1113 552 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7136 7153 7306 7491 7936 325
Size: 1255 5556 17 153 185 445 1498
Fragments arranged by size:
5556 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2317 2710 3111
Size: 274 80 33 51 1443 340 393 401
Cuts at: 3111 3608 4544 4918 6394 7355 9067 96
Size: 497 936 374 1476 961 1712 138
Fragments arranged by size:
1712 1476 1443 961 936 497 401 393
374 340 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3986 4018 4116 4148 4624 4656 9069 9101 3986
Size: 32 98 32 476 32 4413 32 3994
Fragments arranged by size:
4413 3994 476 98 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9109
XbaI T'CTAG_A
Cuts at: 4438 5243 6445 4438
Size: 805 1202 7102
Fragments arranged by size:
7102 1202 805
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9109
XmaI C'CCGG_G
Cuts at: 1870 6238 6434 1870
Size: 4368 196 4545
Fragments arranged by size:
4545 4368 196
XmnI GAAnn'nnTTC
Cuts at: 4284 7238 4284
Size: 2954 6155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6917 276
Size: 53 83 186 936 5383 2468
Fragments arranged by size:
5383 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI