Restriction map for
pFN21ASDA0448
(Circular) (Six-base) MAPSORT of: pFN21ASDA0448.seq Check: 4729 from: 1 to: 5797
pFN21ASDA0448 5797 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2712 2712
Size: 5797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2805 3860 5244 5512 1632
Size: 1173 1055 1384 268 1917
Fragments arranged by size:
1917 1384 1173 1055 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2446 4696 5673 1203
Size: 1243 2250 977 1327
Fragments arranged by size:
2250 1327 1243 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 2168 4526 2168
Size: 2358 3439
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 5765
AlwNI CAG_nnn'CTG
Cuts at: 2065 2122 3540 5112 2065
Size: 57 1418 1572 2750
Fragments arranged by size:
2750 1572 1418 57
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 2471 2975 3106 3332 3484 5593 1493
Size: 978 504 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 978 504 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 2502 2757 4351 160
Size: 872 1470 255 1594 1606
Fragments arranged by size:
1606 1594 1470 872 255
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 2515 3127 3494 2515
Size: 612 367 4818
Fragments arranged by size:
4818 612 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 3010 3116 1391
Size: 640 979 106 4072
Fragments arranged by size:
4072 979 640 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 2247 1548
Size: 699 5098
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 3858 5014 1790
Size: 2068 1156 2573
Fragments arranged by size:
2573 2068 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2637 4457 1239
Size: 905 493 1820 2579
Fragments arranged by size:
2579 1820 905 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3027 4460 882
Size: 617 1528 1433 2219
Fragments arranged by size:
2219 1528 1433 617
BsaAI yAC'GTr
Cuts at: 493 1204 3094 3588 493
Size: 711 1890 494 2702
Fragments arranged by size:
2702 1890 711 494
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2981 1178
Size: 30 165 30 1548 30 3994
Fragments arranged by size:
3994 1548 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2141 1186
Size: 835 120 4842
Fragments arranged by size:
4842 835 120
BseYI C'CCAG_C
Cuts at: 5000 5496 5000
Size: 496 5301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2653 3858 3943 5014 2653
Size: 1205 85 1071 3436
Fragments arranged by size:
3436 1205 1071 85
BsmI GAATG_Cn'
Cuts at: 2708 3308 3401 2708
Size: 600 93 5104
Fragments arranged by size:
5104 600 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2581 2581
Size: 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2653 3010 3116 3858 3943 5014
Size: 399 241 622 357 106 742 85 1071
Cuts at: 5014 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 622 399 357 241 106
85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2712 3152 844
Size: 1868 440 3489
Fragments arranged by size:
3489 1868 440
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 3684 1394
Size: 429 313 1548 3507
Fragments arranged by size:
3507 1548 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 2372 2822 2922 4292 4466 65
Size: 1183 1124 450 100 1370 174 1396
Fragments arranged by size:
1396 1370 1183 1124 450 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 2007 4601 2007
Size: 2594 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2112 2515 3127 3494 3879 3896 5348 5434
Size: 140 403 612 367 385 17 1452 86
Cuts at: 5434 5446 5792 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 612 403 385 367 346 140
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2033 2033
Size: 5797
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4288
Fragments arranged by size:
4288 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2105 487
Size: 683 56 879 4179
Fragments arranged by size:
4179 879 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4107 4127 3313
Size: 794 20 4983
Fragments arranged by size:
4983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2689 3244 4133
Size: 54 1425 50 275 18 859 555 889
Cuts at: 4133 5762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 859 555 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2229 3727 1353
Size: 749 127 1498 3423
Fragments arranged by size:
3423 1498 749 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2637 2805 3860 4457 5244 5512
Size: 393 512 493 168 1055 597 787 268
Cuts at: 5512 1239
Size: 1524
Fragments arranged by size:
1524 1055 787 597 512 493 393 268
168
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 4944 1678
Size: 3266 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2186 4438 4470 4512
Size: 32 135 32 582 32 2252 32 42
Cuts at: 4512 4544 1373
Size: 32 2626
Fragments arranged by size:
2626 2252 582 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2691 10
Size: 54 1425 343 859 3116
Fragments arranged by size:
3116 1425 859 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3755 4114 4273 518
Size: 648 2589 359 159 2042
Fragments arranged by size:
2589 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 3213 3890 5038 5283 1606
Size: 456 1151 677 1148 245 2120
Fragments arranged by size:
2120 1151 1148 677 456 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4288
Fragments arranged by size:
4288 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2122 1108
Size: 1014 4783
PfoI T'CCnGG_A
Cuts at: 2411 2411
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 3866 3898 5379 5411 2951
Size: 32 883 32 1481 32 3337
Fragments arranged by size:
3337 1481 883 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 2256 3367 2256
Size: 1111 4686
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SapI GCTCTTCn'nnn_
Cuts at: 2102 2102
Size: 5797
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2933 3145 4280 4961 5152 826
Size: 219 1888 212 1135 681 191 1471
Fragments arranged by size:
1888 1471 1135 681 219 212 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3901 4802 5064 820
Size: 197 926 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3094 3588 493
Size: 2601 494 2702
Fragments arranged by size:
2702 2601 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 2765 3721 5
Size: 47 2713 956 2081
Fragments arranged by size:
2713 2081 956 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2022 3236 513
Size: 552 957 1214 3074
Fragments arranged by size:
3074 1214 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2661 2661
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 2766 2966 3926 2766
Size: 200 960 4637
Fragments arranged by size:
4637 960 200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI