Restriction map for
pFN21ASDA0441
(Circular) (Six-base) MAPSORT of: pFN21ASDA0441.seq Check: 1365 from: 1 to: 6820
pFN21ASDA0441 6820 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3083 4041 3083
Size: 958 5862
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4163 1937
Size: 2226 4594
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3353 3561 3617 3999 4883 6267 6535 1632
Size: 1721 208 56 382 884 1384 268 1917
Fragments arranged by size:
1917 1721 1384 884 382 268 208 56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 5719 6696 1203
Size: 4516 977 1327
Fragments arranged by size:
4516 1327 977
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 5549 5549
Size: 6820
AleI CACnn'nnGTG
Cuts at: 1166 3309 1166
Size: 2143 4677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6820
AlwNI CAG_nnn'CTG
Cuts at: 4033 4563 6135 4033
Size: 530 1572 4718
Fragments arranged by size:
4718 1572 530
ApaI G_GGCC'C
Cuts at: 2173 3909 2173
Size: 1736 5084
ApaLI G'TGCA_C
Cuts at: 3926 4877 6033 3926
Size: 951 1156 4713
Fragments arranged by size:
4713 1156 951
ApoI r'AATT_y
Cuts at: 1493 2297 3263 3552 3675 3832 4129 4355 4507
Size: 804 966 289 123 157 297 226 152
Cuts at: 4507 6616 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 966 804 297 289 226 157
152 123
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 3661 4145 1870
Size: 73 1718 484 4545
Fragments arranged by size:
4545 1718 484 73
BamHI G'GATC_C
Cuts at: 4150 4517 4150
Size: 367 6453
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 4141 5596 618
Size: 325 217 627 666 1688 1455 1842
Fragments arranged by size:
1842 1688 1455 666 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2173 3909 3996 4139 1391
Size: 782 1736 87 143 4072
Fragments arranged by size:
4072 1736 782 143 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2518 928
Size: 971 619 5230
Fragments arranged by size:
5230 971 619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2016 2050 5011 5045 1903
Size: 34 79 34 2961 34 3678
Fragments arranged by size:
3678 2961 79 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2297 4712 5922 2297
Size: 2415 1210 3195
Fragments arranged by size:
3195 2415 1210
BclI T'GATC_A
Cuts at: 1548 3163 3933 1548
Size: 1615 770 4435
Fragments arranged by size:
4435 1615 770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 2892 5431
Size: 107 122 71 631 605 378 842 2539
Cuts at: 5431 136
Size: 1525
Fragments arranged by size:
2539 1525 842 631 605 378 122 107
71
BglII A'GATC_T
Cuts at: 6815 6815
Size: 6820
BlpI GC'TnA_GC
Cuts at: 4026 4237 4026
Size: 211 6609
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2458 3909 3930 4083 4881 6037 1790
Size: 383 285 1451 21 153 798 1156 2573
Fragments arranged by size:
2573 1451 1156 798 383 285 153 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2453 4551 5509
Size: 416 53 83 399 24 1036 2098 958
Cuts at: 5509 442
Size: 1753
Fragments arranged by size:
2098 1753 1036 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2910 3140 3427 5480 1239
Size: 1671 230 287 2053 2579
Fragments arranged by size:
2579 2053 1671 287 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4945 5810 6108 4945
Size: 865 298 5657
Fragments arranged by size:
5657 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5483 882
Size: 617 3984 2219
Fragments arranged by size:
3984 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3931 4078 4117 4611 493
Size: 711 2727 147 39 494 2702
Fragments arranged by size:
2727 2702 711 494 147 39
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4627 5009 5644 275
Size: 53 83 186 936 3094 382 635 1451
Fragments arranged by size:
3094 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3238 5253 5651 5925 6072 6483 3238
Size: 2015 398 274 147 411 3575
Fragments arranged by size:
3575 2015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6595
Fragments arranged by size:
6595 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2555 2720 2977 3408 4004 1186
Size: 1369 165 257 431 596 4002
Fragments arranged by size:
4002 1369 596 431 257 165
BseYI C'CCAG_C
Cuts at: 4083 6023 6519 4083
Size: 1940 496 4384
Fragments arranged by size:
4384 1940 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3061 3106 3119 1163
Size: 573 1325 45 13 4864
Fragments arranged by size:
4864 1325 573 45 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3068 4270 5031 6059 1540
Size: 275 186 1067 1202 761 1028 2301
Fragments arranged by size:
2301 1202 1067 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3126 3930 3996 4881 4966 6037 3126
Size: 804 66 885 85 1071 3909
Fragments arranged by size:
3909 1071 885 804 85 66
BsmI GAATG_Cn'
Cuts at: 3248 3465 4331 4424 3248
Size: 217 866 93 5644
Fragments arranged by size:
5644 866 217 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2458 3126 3909 3930 3996 4083
Size: 399 383 285 668 783 21 66 87
Cuts at: 4083 4139 4881 4966 6037 1391
Size: 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 668 399 383 285
87 85 66 56 21
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 4709 6439 4709
Size: 1730 5090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3083 4041 4175 844
Size: 2239 958 134 3489
Fragments arranged by size:
3489 2239 958 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3064 4707 1394
Size: 429 1241 1643 3507
Fragments arranged by size:
3507 1643 1241 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2531 5315 5489 65
Size: 1183 1283 2784 174 1396
Fragments arranged by size:
2784 1396 1283 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5464 5651 1700
Size: 110 3654 187 2869
Fragments arranged by size:
3654 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2062 2158 3700 96
Size: 1966 96 1542 3216
Fragments arranged by size:
3216 1966 1542 96
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 4880 5892 4880
Size: 1012 5808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3084 3115 3252 794
Size: 2290 31 137 4362
Fragments arranged by size:
4362 2290 137 31
BstBI TT'CG_AA
Cuts at: 2007 5624 2007
Size: 3617 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6820
BstYI r'GATC_y
Cuts at: 1972 2801 2865 4018 4150 4517 4902 4919 6371
Size: 829 64 1153 132 367 385 17 1452
Cuts at: 6371 6457 6469 6815 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1153 829 385 367 346 132
86 64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2898 2898
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2022 2848 513
Size: 552 957 826 4485
Fragments arranged by size:
4485 957 826 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2019 3242 487
Size: 683 56 793 1223 4065
Fragments arranged by size:
4065 1223 793 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 3113 3460 4336 5130 5150 2445
Size: 668 347 876 794 20 4115
Fragments arranged by size:
4115 876 794 668 347 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6785
DraI TTT'AAA
Cuts at: 4124 4124
Size: 6820
DraIII CAC_nnn'GTG
Cuts at: 3084 4078 3084
Size: 994 5826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5827 809
Size: 507 4511 1802
Fragments arranged by size:
4511 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2239 2758
Size: 54 1425 50 275 18 264 145 519
Cuts at: 2758 3065 4099 4267 5156 6785 8
Size: 307 1034 168 889 1629 43
Fragments arranged by size:
1629 1425 1034 889 519 307 275 264
168 145 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3065 4267 1537
Size: 275 1253 1202 4090
Fragments arranged by size:
4090 1253 1202 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 3372 3609 4750 1353
Size: 867 1152 237 1141 3423
Fragments arranged by size:
3423 1152 1141 867 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4047 5407 5781 5927 387
Size: 1064 138 2458 1360 374 146 1280
Fragments arranged by size:
2458 1360 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3994 3994
Size: 6820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2910 3140 3353 3427 3561 3617 3999
Size: 393 1278 230 213 74 134 56 382
Cuts at: 3999 4883 5480 6267 6535 1239
Size: 884 597 787 268 1524
Fragments arranged by size:
1524 1278 884 787 597 393 382 268
230 213 134 74 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2387 2387
Size: 6820
EcoO109I rG'GnC_Cy
Cuts at: 2894 3905 3906 2894
Size: 1011 1 5808
Fragments arranged by size:
5808 1011 1
EcoRI G'AATT_C
Cuts at: 4129 4507 4129
Size: 378 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2201 3562 2140
Size: 61 1361 5398
Fragments arranged by size:
5398 1361 61
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 2887 5967 1678
Size: 1209 3080 2531
Fragments arranged by size:
3080 2531 1209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2531 2563 5461 5493 5535
Size: 32 135 32 959 32 2898 32 42
Cuts at: 5535 5567 1373
Size: 32 2626
Fragments arranged by size:
2898 2626 959 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4164 4410 669
Size: 988 281 2226 246 3079
Fragments arranged by size:
3079 2226 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 4410 4410
Size: 6820
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5934 6118 1305
Size: 4629 184 2007
Fragments arranged by size:
4629 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2760 4101 10
Size: 54 1425 343 264 664 1341 2729
Fragments arranged by size:
2729 1425 1341 664 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2325 3047 3272 3309 3966 4778
Size: 648 1086 73 722 225 37 657 812
Cuts at: 4778 5137 5296 518
Size: 359 159 2042
Fragments arranged by size:
2042 1086 812 722 657 648 359 225
159 73 37
MspA1I CmG'CkG
Cuts at: 1606 2907 4039 4236 4913 6061 6306 1606
Size: 1301 1132 197 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 1132 677 245 197
NcoI C'CATG_G
Cuts at: 513 1065 2022 2848 513
Size: 552 957 826 4485
Fragments arranged by size:
4485 957 826 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NspI r_CATG'y
Cuts at: 1132 1229 3575 4178 5723 1132
Size: 97 2346 603 1545 2229
Fragments arranged by size:
2346 2229 1545 603 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6820
PciI A'CATG_T
Cuts at: 5719 5719
Size: 6820
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6820
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PmlI CAC'GTG
Cuts at: 3931 4078 3931
Size: 147 6673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3148 1613
Size: 1535 5285
PsiI TTA'TAA
Cuts at: 4390 4390
Size: 6820
PspOMI G'GGCC_C
Cuts at: 2169 3905 2169
Size: 1736 5084
PstI C_TGCA'G
Cuts at: 830 2563 3757 3781 3831 4038 4172 830
Size: 1733 1194 24 50 207 134 3478
Fragments arranged by size:
3478 1733 1194 207 134 50 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 4039 4039
Size: 6820
SacI G_AGCT'C
Cuts at: 3996 3996
Size: 6820
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 4012 5068 6780 4012
Size: 1056 1712 4052
Fragments arranged by size:
4052 1712 1056
SexAI A'CCwGG_T
Cuts at: 2382 2382
Size: 6820
SfcI C'TryA_G
Cuts at: 826 1045 2559 3032 3638 3753 3777 3827 4034
Size: 219 1514 473 606 115 24 50 207
Cuts at: 4034 4168 5303 5984 6175 826
Size: 134 1135 681 191 1471
Fragments arranged by size:
1514 1471 1135 681 606 473 219 207
191 134 115 50 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 4147 1872
Size: 2275 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4924 5825 6087 820
Size: 197 926 2981 901 262 1553
Fragments arranged by size:
2981 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4117 4611 493
Size: 3624 494 2702
Fragments arranged by size:
3624 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 3575 4178 1132
Size: 2443 603 3774
Fragments arranged by size:
3774 2443 603
SspI AAT'ATT
Cuts at: 5 52 3179 3628 4744 5
Size: 47 3127 449 1116 2081
Fragments arranged by size:
3127 2081 1116 449 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6820
StyI C'CwwG_G
Cuts at: 513 1065 2022 2848 3945 4259 513
Size: 552 957 826 1097 314 3074
Fragments arranged by size:
3074 1097 957 826 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 4081 4847 4864 5017 5202 5647
Size: 1255 1185 1316 766 17 153 185 445
Cuts at: 5647 325
Size: 1498
Fragments arranged by size:
1498 1316 1255 1185 766 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2062 2158 2336
Size: 274 80 33 51 1443 85 96 178
Cuts at: 2336 2638 2934 3700 4010 5066 6778 96
Size: 302 296 766 310 1056 1712 138
Fragments arranged by size:
1712 1443 1056 766 310 302 296 274
178 138 96 85 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6780 6812 6780
Size: 32 6788
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6820
XbaI T'CTAG_A
Cuts at: 4156 4156
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 3952 2254
Size: 1698 5122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 4145 1870
Size: 2275 4545
XmnI GAAnn'nnTTC
Cuts at: 2123 3488 3870 3963 4949 2123
Size: 1365 382 93 986 3994
Fragments arranged by size:
3994 1365 986 382 93
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI