Restriction map for
pFN21ASDA0438
(Circular) (Six-base) MAPSORT of: pFN21ASDA0438.seq Check: 7021 from: 1 to: 6853
pFN21ASDA0438 6853 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4069 4069
Size: 6853
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4663 278
Size: 53 83 186 936 3127 2468
Fragments arranged by size:
3127 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4196 1937
Size: 2259 4594
Acc65I G'GTAC_C
Cuts at: 4174 4174
Size: 6853
AclI AA'CG_TT
Cuts at: 1563 4980 5353 1563
Size: 3417 373 3063
Fragments arranged by size:
3417 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2892 3467 3477 3732 4916 6300 6568 1632
Size: 1260 575 10 255 1184 1384 268 1917
Fragments arranged by size:
1917 1384 1260 1184 575 268 255 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6656
AflIII A'CryG_T
Cuts at: 1203 5752 6729 1203
Size: 4549 977 1327
Fragments arranged by size:
4549 1327 977
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 5582 5582
Size: 6853
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 6821
AlwNI CAG_nnn'CTG
Cuts at: 4596 6168 4596
Size: 1572 5281
ApaLI G'TGCA_C
Cuts at: 4910 6066 4910
Size: 1156 5697
ApoI r'AATT_y
Cuts at: 1493 2245 2325 2787 3519 3587 4162 4388 4540
Size: 752 80 462 732 68 575 226 152
Cuts at: 4540 6649 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 752 732 575 462 226 152
80 68
AseI AT'TA_AT
Cuts at: 160 1032 5407 160
Size: 872 4375 1606
Fragments arranged by size:
4375 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 4178 1870
Size: 73 2235 4545
Fragments arranged by size:
4545 2235 73
BamHI G'GATC_C
Cuts at: 3151 3730 4183 4550 3151
Size: 579 453 367 5454
Fragments arranged by size:
5454 579 453 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4174 5629 618
Size: 325 217 627 2387 1455 1842
Fragments arranged by size:
2387 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4172 1391
Size: 2781 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2143 2790 3660 3860 928
Size: 971 244 647 870 200 3921
Fragments arranged by size:
3921 971 870 647 244 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5044 5078 1903
Size: 34 3107 34 3678
Fragments arranged by size:
3678 3107 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2404 4745 5955 2404
Size: 2341 1210 3302
Fragments arranged by size:
3302 2341 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6853
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5464 136
Size: 107 122 71 631 605 3792 1525
Fragments arranged by size:
3792 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6848 6848
Size: 6853
BlpI GC'TnA_GC
Cuts at: 3769 4270 3769
Size: 501 6352
Bme1580I G_kGCm'C
Cuts at: 1790 4914 6070 1790
Size: 3124 1156 2573
Fragments arranged by size:
3124 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6853
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2286 4584 5542
Size: 416 53 83 399 24 869 2298 958
Cuts at: 5542 442
Size: 1753
Fragments arranged by size:
2298 1753 958 869 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2817 3655 2817
Size: 838 6015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3625 3650 5513 1239
Size: 2386 25 1863 2579
Fragments arranged by size:
2579 2386 1863 25
Bpu10I CC'TnA_GC
Cuts at: 2188 3429 2188
Size: 1241 5612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2787 3148 3364 4978 5843 6141 2787
Size: 361 216 1614 865 298 3499
Fragments arranged by size:
3499 1614 865 361 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3869 5516 882
Size: 617 2370 1647 2219
Fragments arranged by size:
2370 2219 1647 617
BsaAI yAC'GTr
Cuts at: 493 1204 4150 4644 493
Size: 711 2946 494 2702
Fragments arranged by size:
2946 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6847 6847
Size: 6853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4660 5042 5677 275
Size: 53 83 186 936 3127 382 635 1451
Fragments arranged by size:
3127 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5286 5684 5958 6105 6516 5286
Size: 398 274 147 411 5623
Fragments arranged by size:
5623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6628
Fragments arranged by size:
6628 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6853
BseYI C'CCAG_C
Cuts at: 3393 6056 6552 3393
Size: 2663 496 3694
Fragments arranged by size:
3694 2663 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6280
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4303 5064 6092 1540
Size: 275 186 2302 761 1028 2301
Fragments arranged by size:
2302 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4914 4999 6070 4914
Size: 85 1071 5697
Fragments arranged by size:
5697 1071 85
BsmI GAATG_Cn'
Cuts at: 3317 4364 4457 3317
Size: 1047 93 5713
Fragments arranged by size:
5713 1047 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4172 4914 4999 6070 1391
Size: 399 2382 742 85 1071 2174
Fragments arranged by size:
2382 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6516 6516
Size: 6853
BspHI T'CATG_A
Cuts at: 4742 6472 4742
Size: 1730 5123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3586 4069 4208 844
Size: 2742 483 139 3489
Fragments arranged by size:
3489 2742 483 139
BsrBI CCG'CTC
Cuts at: 1394 1823 4740 1394
Size: 429 2917 3507
Fragments arranged by size:
3507 2917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2061 5348 5522 65
Size: 1183 813 3287 174 1396
Fragments arranged by size:
3287 1396 1183 813 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5497 5684 1700
Size: 110 3687 187 2869
Fragments arranged by size:
3687 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6853
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6853
BssSI C'ACGA_G
Cuts at: 4913 5925 4913
Size: 1012 5841
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 2007 5657 2007
Size: 3650 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6853
BstXI CCAn_nnnn'nTGG
Cuts at: 2069 2266 2069
Size: 197 6656
BstYI r'GATC_y
Cuts at: 1972 3151 3730 4183 4550 4935 4952 6404 6490
Size: 1179 579 453 367 385 17 1452 86
Cuts at: 6490 6502 6848 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1179 579 453 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2251 2416 2251
Size: 165 6688
BtgI C'CryG_G
Cuts at: 513 1065 2938 513
Size: 552 1873 4428
Fragments arranged by size:
4428 1873 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6114
Fragments arranged by size:
6114 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 3454 3565 3822 3897 3943 4369 5163 5183
Size: 132 111 257 75 46 426 794 20
Cuts at: 5183 3322
Size: 4992
Fragments arranged by size:
4992 794 426 257 132 111 75 46
20
ClaI AT'CG_AT
Cuts at: 1554 3349 1554
Size: 1795 5058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2982 3017 529
Size: 35 2418 35 4365
Fragments arranged by size:
4365 2418 35 35
DraI TTT'AAA
Cuts at: 2158 4157 2158
Size: 1999 4854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5860 809
Size: 507 4544 1802
Fragments arranged by size:
4544 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3831 4300 5189
Size: 54 1425 50 275 18 2001 469 889
Cuts at: 5189 6818 8
Size: 1629 43
Fragments arranged by size:
2001 1629 1425 889 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4300 1537
Size: 275 2488 4090
Fragments arranged by size:
4090 2488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 3486 3504 4783 1353
Size: 1356 777 18 1279 3423
Fragments arranged by size:
3423 1356 1279 777 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5440 5814 5960 387
Size: 1064 138 3851 374 146 1280
Fragments arranged by size:
3851 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2892 3467 3477 3625 3650 3732 4916
Size: 393 1260 575 10 148 25 82 1184
Cuts at: 4916 5513 6300 6568 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1260 1184 787 597 575 393 268
148 82 25 10
EcoRI G'AATT_C
Cuts at: 4162 4540 4162
Size: 378 6475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2160 2160
Size: 6853
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 6000 1678
Size: 4322 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 3362 3394 5494
Size: 32 135 32 479 32 1279 32 2100
Cuts at: 5494 5526 5568 5600 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2100 1279 479 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4197 4443 669
Size: 988 281 2259 246 3079
Fragments arranged by size:
3079 2259 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2185 748
Size: 1437 5416
HpaI GTT'AAC
Cuts at: 4443 4443
Size: 6853
KpnI G_GTAC'C
Cuts at: 4178 4178
Size: 6853
MfeI C'AATT_G
Cuts at: 4452 4452
Size: 6853
MluI A'CGCG_T
Cuts at: 6729 6729
Size: 6853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 2613 3591 5967 6151 1305
Size: 894 414 978 2376 184 2007
Fragments arranged by size:
2376 2007 978 894 414 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3833 10
Size: 54 1425 343 2001 3030
Fragments arranged by size:
3030 2001 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2394 4811 5170 5329 518
Size: 648 979 249 2417 359 159 2042
Fragments arranged by size:
2417 2042 979 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 3227 4269 4946 6094 6339 1606
Size: 1621 1042 677 1148 245 2120
Fragments arranged by size:
2120 1621 1148 1042 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2938 513
Size: 552 1873 4428
Fragments arranged by size:
4428 1873 552
NdeI CA'TA_TG
Cuts at: 387 3724 387
Size: 3337 3516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 4300 4300
Size: 6853
NspI r_CATG'y
Cuts at: 1132 1229 2144 4035 4211 5756 1132
Size: 97 915 1891 176 1545 2229
Fragments arranged by size:
2229 1891 1545 915 176 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6853
PciI A'CATG_T
Cuts at: 5752 5752
Size: 6853
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3781 1108
Size: 2673 4180
PfoI T'CCnGG_A
Cuts at: 2464 2464
Size: 6853
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4922 4954 6435 6467 4922
Size: 32 1481 32 5308
Fragments arranged by size:
5308 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6853
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3417 3449 3417
Size: 32 6821
PstI C_TGCA'G
Cuts at: 830 2578 4084 4205 830
Size: 1748 1506 121 3478
Fragments arranged by size:
3478 1748 1506 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
SalI G'TCGA_C
Cuts at: 1936 4195 1936
Size: 2259 4594
SbfI CC_TGCA'GG
Cuts at: 4205 4205
Size: 6853
ScaI AGT'ACT
Cuts at: 5101 6813 5101
Size: 1712 5141
SfcI C'TryA_G
Cuts at: 826 1045 2574 4080 4201 5336 6017 6208 826
Size: 219 1529 1506 121 1135 681 191 1471
Fragments arranged by size:
1529 1506 1471 1135 681 219 191 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6853
SmaI CCC'GGG
Cuts at: 1872 4180 1872
Size: 2308 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2802 3163 3379 4957 5858 6120
Size: 197 926 859 361 216 1578 901 262
Cuts at: 6120 820
Size: 1553
Fragments arranged by size:
1578 1553 926 901 859 361 262 216
197
SnaBI TAC'GTA
Cuts at: 493 4150 4644 493
Size: 3657 494 2702
Fragments arranged by size:
3657 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 4211 1132
Size: 3079 3774
SspI AAT'ATT
Cuts at: 5 52 4777 5
Size: 47 4725 2081
Fragments arranged by size:
4725 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6853
StyI C'CwwG_G
Cuts at: 513 1065 2938 3504 3524 4292 513
Size: 552 1873 566 20 768 3074
Fragments arranged by size:
3074 1873 768 566 552 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4880 4897 5050 5235 5680 325
Size: 1255 3300 17 153 185 445 1498
Fragments arranged by size:
3300 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 2386 2426
Size: 274 80 33 51 1443 54 355 40
Cuts at: 2426 3534 5099 6811 96
Size: 1108 1565 1712 138
Fragments arranged by size:
1712 1565 1443 1108 355 274 138 80
54 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3250 3282 6813 6845 3250
Size: 32 3531 32 3258
Fragments arranged by size:
3531 3258 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6853
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6853
XmaI C'CCGG_G
Cuts at: 1870 4178 1870
Size: 2308 4545
XmnI GAAnn'nnTTC
Cuts at: 4982 4982
Size: 6853
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4661 276
Size: 53 83 186 936 3127 2468
Fragments arranged by size:
3127 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI