Restriction map for
pFN21ASDA0436
(Circular) (Six-base) MAPSORT of: pFN21ASDA0436.seq Check: 4649 from: 1 to: 6643
pFN21ASDA0436 6643 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4453 278
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3986 1937
Size: 2049 4594
Acc65I G'GTAC_C
Cuts at: 3697 3964 3697
Size: 267 6376
AclI AA'CG_TT
Cuts at: 1563 3619 4770 5143 1563
Size: 2056 1151 373 3063
Fragments arranged by size:
3063 2056 1151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 2313 2601 2864 4706 6090 6358 1632
Size: 672 9 288 263 1842 1384 268 1917
Fragments arranged by size:
1917 1842 1384 672 288 268 263 9
AflII C'TTAA_G
Cuts at: 820 1017 2207 3917 820
Size: 197 1190 1710 3546
Fragments arranged by size:
3546 1710 1190 197
AflIII A'CryG_T
Cuts at: 1203 2814 3184 5542 6519 1203
Size: 1611 370 2358 977 1327
Fragments arranged by size:
2358 1611 1327 977 370
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6643
AlwNI CAG_nnn'CTG
Cuts at: 2741 4386 5958 2741
Size: 1645 1572 3426
Fragments arranged by size:
3426 1645 1572
ApaLI G'TGCA_C
Cuts at: 4700 5856 4700
Size: 1156 5487
ApoI r'AATT_y
Cuts at: 1493 2717 2947 3220 3866 3932 3952 4178 4330
Size: 1224 230 273 646 66 20 226 152
Cuts at: 4330 6439 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1224 646 273 230 226 152
66 20
AseI AT'TA_AT
Cuts at: 160 1032 5197 160
Size: 872 4165 1606
Fragments arranged by size:
4165 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 3968 1870
Size: 73 2025 4545
Fragments arranged by size:
4545 2025 73
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3502 3697 3964 5419 618
Size: 325 217 627 1715 195 267 1455 1842
Fragments arranged by size:
1842 1715 1455 627 325 267 217 195
BanII G_rGCy'C
Cuts at: 1391 2609 3962 1391
Size: 1218 1353 4072
Fragments arranged by size:
4072 1353 1218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3063 928
Size: 971 1164 4508
Fragments arranged by size:
4508 1164 971
BbvCI CC'TCA_GC
Cuts at: 3648 3648
Size: 6643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4834 4868 1903
Size: 34 2897 34 3678
Fragments arranged by size:
3678 2897 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2935 4535 5745 2935
Size: 1600 1210 3833
Fragments arranged by size:
3833 1600 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6643
BfrBI ATG'CAT
Cuts at: 2030 2030
Size: 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3235 5254 136
Size: 107 122 71 631 605 1563 2019 1525
Fragments arranged by size:
2019 1563 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6638 6638
Size: 6643
BlpI GC'TnA_GC
Cuts at: 2321 4060 2321
Size: 1739 4904
Bme1580I G_kGCm'C
Cuts at: 1790 4704 5860 1790
Size: 2914 1156 2573
Fragments arranged by size:
2914 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3057 4374 5332
Size: 416 53 83 399 24 1640 1317 958
Cuts at: 5332 442
Size: 1753
Fragments arranged by size:
1753 1640 1317 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 3290 3456 3834 5303 1239
Size: 993 1058 166 378 1469 2579
Fragments arranged by size:
2579 1469 1058 993 378 166
Bpu10I CC'TnA_GC
Cuts at: 2603 3648 3759 2603
Size: 1045 111 5487
Fragments arranged by size:
5487 1045 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3719 4768 5633 5931 3719
Size: 1049 865 298 4431
Fragments arranged by size:
4431 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2907 5306 882
Size: 617 1408 2399 2219
Fragments arranged by size:
2399 2219 1408 617
BsaAI yAC'GTr
Cuts at: 493 1204 3940 4434 493
Size: 711 2736 494 2702
Fragments arranged by size:
2736 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6637 6637
Size: 6643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4450 4832 5467 275
Size: 53 83 186 936 2917 382 635 1451
Fragments arranged by size:
2917 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5076 5474 5748 5895 6306 5076
Size: 398 274 147 411 5413
Fragments arranged by size:
5413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6418
Fragments arranged by size:
6418 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6643
BseYI C'CCAG_C
Cuts at: 5846 6342 5846
Size: 496 6147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6070
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4093 4854 5882 1540
Size: 275 186 2092 761 1028 2301
Fragments arranged by size:
2301 2092 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4704 4789 5860 4704
Size: 85 1071 5487
Fragments arranged by size:
5487 1071 85
BsmI GAATG_Cn'
Cuts at: 4154 4247 4154
Size: 93 6550
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2609 3962 4704 4789 5860 1391
Size: 399 819 1353 742 85 1071 2174
Fragments arranged by size:
2174 1353 1071 819 742 399 85
BspEI T'CCGG_A
Cuts at: 6306 6306
Size: 6643
BspHI T'CATG_A
Cuts at: 2437 4532 6262 2437
Size: 2095 1730 2818
Fragments arranged by size:
2818 2095 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3998 844
Size: 3154 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3488 4530 1394
Size: 429 1665 1042 3507
Fragments arranged by size:
3507 1665 1042 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5138 5312 65
Size: 1183 3890 174 1396
Fragments arranged by size:
3890 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5287 5474 1700
Size: 110 3477 187 2869
Fragments arranged by size:
3477 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 4703 5715 4703
Size: 1012 5631
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 2007 5447 2007
Size: 3440 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstYI r'GATC_y
Cuts at: 1972 3913 3973 4340 4725 4742 6194 6280 6292
Size: 1941 60 367 385 17 1452 86 12
Cuts at: 6292 6638 1972
Size: 346 1977
Fragments arranged by size:
1977 1941 1452 385 367 346 86 60
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5134
Fragments arranged by size:
5134 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3730 487
Size: 683 56 2504 3400
Fragments arranged by size:
3400 2504 683 56
BtsI GCAGTG_nn'
Cuts at: 4159 4953 4973 4159
Size: 794 20 5829
Fragments arranged by size:
5829 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3947 3947
Size: 6643
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5650 809
Size: 507 4334 1802
Fragments arranged by size:
4334 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3322 3385 4090
Size: 54 1425 50 275 18 1492 63 705
Cuts at: 4090 4979 6608 8
Size: 889 1629 43
Fragments arranged by size:
1629 1492 1425 889 705 275 63 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4090 1537
Size: 275 2278 4090
Fragments arranged by size:
4090 2278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2780 4573 1353
Size: 1427 1793 3423
Fragments arranged by size:
3423 1793 1427
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5230 5604 5750 387
Size: 1064 138 3641 374 146 1280
Fragments arranged by size:
3641 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2304 2313 2601 2864 3290 3456
Size: 393 600 72 9 288 263 426 166
Cuts at: 3456 3834 4706 5303 6090 6358 1239
Size: 378 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 600 597 426 393 378
288 268 263 166 72 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2419 3705 3875 2419
Size: 1286 170 5187
Fragments arranged by size:
5187 1286 170
EcoO109I rG'GnC_Cy
Cuts at: 2614 2650 3241 2614
Size: 36 591 6016
Fragments arranged by size:
6016 591 36
EcoRI G'AATT_C
Cuts at: 3952 4330 3952
Size: 378 6265
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 5790 1678
Size: 4112 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5284 5316 5358 5390 1373
Size: 32 135 32 3712 32 42 32 2626
Fragments arranged by size:
3712 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3987 4233 669
Size: 988 281 2049 246 3079
Fragments arranged by size:
3079 2049 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2343 748
Size: 1595 5048
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KpnI G_GTAC'C
Cuts at: 3701 3968 3701
Size: 267 6376
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2683 5757 5941 1305
Size: 1378 3074 184 2007
Fragments arranged by size:
3074 2007 1378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3387 10
Size: 54 1425 343 1555 3266
Fragments arranged by size:
3266 1555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2673 3189 3318 3530 4601 4960
Size: 648 924 583 516 129 212 1071 359
Cuts at: 4960 5119 518
Size: 159 2042
Fragments arranged by size:
2042 1071 924 648 583 516 359 212
159 129
MspA1I CmG'CkG
Cuts at: 1606 2738 4059 4736 5884 6129 1606
Size: 1132 1321 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 1132 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5134
Fragments arranged by size:
5134 957 552
NdeI CA'TA_TG
Cuts at: 387 3679 387
Size: 3292 3351
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NsiI A_TGCA'T
Cuts at: 2032 2032
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 2818 3188 3758 4001 5546 1132
Size: 97 1589 370 570 243 1545 2229
Fragments arranged by size:
2229 1589 1545 570 370 243 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6643
PciI A'CATG_T
Cuts at: 2814 3184 5542 2814
Size: 370 2358 3915
Fragments arranged by size:
3915 2358 370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2614 2650 3241 2614
Size: 36 591 6016
Fragments arranged by size:
6016 591 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 2315 4213 2315
Size: 1898 4745
PstI C_TGCA'G
Cuts at: 830 3995 830
Size: 3165 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SanDI GG'GwC_CC
Cuts at: 2614 2614
Size: 6643
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 2625 4891 6603 2625
Size: 2266 1712 2665
Fragments arranged by size:
2665 2266 1712
SfcI C'TryA_G
Cuts at: 826 1045 2712 3991 5126 5807 5998 826
Size: 219 1667 1279 1135 681 191 1471
Fragments arranged by size:
1667 1471 1279 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 3970 1872
Size: 2098 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 3734 3917 4747 5648 5910
Size: 197 926 264 1527 183 830 901 262
Cuts at: 5910 820
Size: 1553
Fragments arranged by size:
1553 1527 926 901 830 264 262 197
183
SnaBI TAC'GTA
Cuts at: 493 3940 4434 493
Size: 3447 494 2702
Fragments arranged by size:
3447 2702 494
SpeI A'CTAG_T
Cuts at: 152 3074 152
Size: 2922 3721
SphI G_CATG'C
Cuts at: 1132 3758 4001 1132
Size: 2626 243 3774
Fragments arranged by size:
3774 2626 243
SspI AAT'ATT
Cuts at: 5 52 2560 3005 3640 3793 4567 5
Size: 47 2508 445 635 153 774 2081
Fragments arranged by size:
2508 2081 774 635 445 153 47
StuI AGG'CCT
Cuts at: 1517 3229 3303 1517
Size: 1712 74 4857
Fragments arranged by size:
4857 1712 74
StyI C'CwwG_G
Cuts at: 513 1065 2022 2609 2918 3388 4082 513
Size: 552 957 587 309 470 694 3074
Fragments arranged by size:
3074 957 694 587 552 470 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2889 4670 4687 4840 5025 5470 325
Size: 1255 1309 1781 17 153 185 445 1498
Fragments arranged by size:
1781 1498 1309 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2623 4889 6601
Size: 274 80 33 51 1443 646 2266 1712
Cuts at: 6601 96
Size: 138
Fragments arranged by size:
2266 1712 1443 646 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3105 3137 3529 3561 6603 6635 3105
Size: 32 392 32 3042 32 3113
Fragments arranged by size:
3113 3042 392 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3091 3799 3979 3091
Size: 708 180 5755
Fragments arranged by size:
5755 708 180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3284 3284
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 3968 1870
Size: 2098 4545
XmnI GAAnn'nnTTC
Cuts at: 4772 4772
Size: 6643
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4451 276
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI