Restriction map for
pFN21ASDA0422
(Circular) (Six-base) MAPSORT of: pFN21ASDA0422.seq Check: 6934 from: 1 to: 8074
pFN21ASDA0422 8074 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2117 2383 3021 4597 5135 2117
Size: 266 638 1576 538 5056
Fragments arranged by size:
5056 1576 638 538 266
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5884 278
Size: 53 83 186 936 4348 2468
Fragments arranged by size:
4348 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3378 4616 5417 1937
Size: 823 618 1238 801 4594
Fragments arranged by size:
4594 1238 823 801 618
Acc65I G'GTAC_C
Cuts at: 5395 5395
Size: 8074
AclI AA'CG_TT
Cuts at: 1563 6201 6574 1563
Size: 4638 373 3063
Fragments arranged by size:
4638 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 6137 7521 7789 1632
Size: 4505 1384 268 1917
Fragments arranged by size:
4505 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7877
AflIII A'CryG_T
Cuts at: 1203 3639 4302 6973 7950 1203
Size: 2436 663 2671 977 1327
Fragments arranged by size:
2671 2436 1327 977 663
AgeI A'CCGG_T
Cuts at: 6905 6905
Size: 8074
AhdI GACnn_n'nnGTC
Cuts at: 6803 6803
Size: 8074
AleI CACnn'nnGTG
Cuts at: 1166 2513 3360 1166
Size: 1347 847 5880
Fragments arranged by size:
5880 1347 847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2907 3220 4154 1184
Size: 1723 313 934 5104
Fragments arranged by size:
5104 1723 934 313
AlwNI CAG_nnn'CTG
Cuts at: 3220 3592 5817 7389 3220
Size: 372 2225 1572 3905
Fragments arranged by size:
3905 2225 1572 372
ApaI G_GGCC'C
Cuts at: 2201 5135 5360 2201
Size: 2934 225 4915
Fragments arranged by size:
4915 2934 225
ApaLI G'TGCA_C
Cuts at: 3205 3461 6131 7287 3205
Size: 256 2670 1156 3992
Fragments arranged by size:
3992 2670 1156 256
ApoI r'AATT_y
Cuts at: 1493 5383 5609 5761 7870 1493
Size: 3890 226 152 2109 1697
Fragments arranged by size:
3890 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6628 160
Size: 872 5596 1606
Fragments arranged by size:
5596 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8074
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 3339 3573 3738 4749 5399 1870
Size: 73 178 1218 234 165 1011 650 4545
Fragments arranged by size:
4545 1218 1011 650 234 178 165 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2292 2325 3572 3605 2292
Size: 33 1247 33 6761
Fragments arranged by size:
6761 1247 33 33
BamHI G'GATC_C
Cuts at: 4018 5404 5771 4018
Size: 1386 367 6321
Fragments arranged by size:
6321 1386 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2208 2341 2353 2379
Size: 325 217 627 322 99 133 12 26
Cuts at: 2379 2742 2891 3683 3860 5395 6850 618
Size: 363 149 792 177 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 792 627 363 325 322
217 177 149 133 99 26 12
BanII G_rGCy'C
Cuts at: 1391 2201 2604 3243 4404 5135 5161 5360 5393
Size: 810 403 639 1161 731 26 199 33
Cuts at: 5393 1391
Size: 4072
Fragments arranged by size:
4072 1161 810 731 639 403 199 33
26
BbeI G_GCGC'C
Cuts at: 2113 2345 2383 3687 2113
Size: 232 38 1304 6500
Fragments arranged by size:
6500 1304 232 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3096 3117 4051 4342 928
Size: 971 1197 21 934 291 4660
Fragments arranged by size:
4660 1197 971 934 291 21
BbvCI CC'TCA_GC
Cuts at: 2334 2550 2807 2334
Size: 216 257 7601
Fragments arranged by size:
7601 257 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3008 3042 3719 3753 6265 6299 1903
Size: 34 1071 34 677 34 2512 34 3678
Fragments arranged by size:
3678 2512 1071 677 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2293 5966 7176 2293
Size: 3673 1210 3191
Fragments arranged by size:
3673 3191 1210
BclI T'GATC_A
Cuts at: 1548 3934 1548
Size: 2386 5688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2564 3555 3627
Size: 107 122 71 631 605 892 991 72
Cuts at: 3627 3719 3828 6685 136
Size: 92 109 2857 1525
Fragments arranged by size:
2857 1525 991 892 631 605 122 109
107 92 72 71
BglII A'GATC_T
Cuts at: 3129 3294 8069 3129
Size: 165 4775 3134
Fragments arranged by size:
4775 3134 165
BlpI GC'TnA_GC
Cuts at: 4602 5491 4602
Size: 889 7185
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2213 2358 2747 3209 3465 3863 4051
Size: 411 12 145 389 462 256 398 188
Cuts at: 4051 4243 5135 5360 6135 7291 1790
Size: 192 892 225 775 1156 2573
Fragments arranged by size:
2573 1156 892 775 462 411 398 389
256 225 192 188 145 12
BmgBI CAC'GTC
Cuts at: 1318 4729 1318
Size: 3411 4663
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3196 5129 5805
Size: 416 53 83 399 24 1779 1933 676
Cuts at: 5805 6763 442
Size: 958 1753
Fragments arranged by size:
1933 1779 1753 958 676 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8074
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 8074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 4868 5312 6734 1239
Size: 1211 2418 444 1422 2579
Fragments arranged by size:
2579 2418 1422 1211 444
Bpu10I CC'TnA_GC
Cuts at: 2334 2550 2807 3905 2334
Size: 216 257 1098 6503
Fragments arranged by size:
6503 1098 257 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3632 4013 6199 7064 7362 3632
Size: 381 2186 865 298 4344
Fragments arranged by size:
4344 2186 865 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2975 4493 6737 882
Size: 617 1476 1518 2244 2219
Fragments arranged by size:
2244 2219 1518 1476 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 3407 5371 5865 493
Size: 711 1458 745 1964 494 2702
Fragments arranged by size:
2702 1964 1458 745 711 494
BsaBI GATnn'nnATC
Cuts at: 3293 8068 3293
Size: 4775 3299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2110 2226 2342 2380
Size: 53 83 186 936 577 116 116 38
Cuts at: 2380 3471 3684 4473 5881 6263 6898 275
Size: 1091 213 789 1408 382 635 1451
Fragments arranged by size:
1451 1408 1091 936 789 635 577 382
213 186 116 116 83 53 38
BsaWI w'CCGG_w
Cuts at: 2235 4695 6507 6905 7179 7326 7737 2235
Size: 2460 1812 398 274 147 411 2572
Fragments arranged by size:
2572 2460 1812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 4023 4053 1178
Size: 30 165 30 1020 30 1570 30 5199
Fragments arranged by size:
5199 1570 1020 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 3719 4682 4691 4940 1186
Size: 1582 951 963 9 249 4320
Fragments arranged by size:
4320 1582 963 951 249 9
BseYI C'CCAG_C
Cuts at: 2180 2266 2668 3063 3771 4426 4435 5360 7277
Size: 86 402 395 708 655 9 925 1917
Cuts at: 7277 7773 2180
Size: 496 2481
Fragments arranged by size:
2481 1917 925 708 655 496 402 395
86 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2122 2411 2708 2899 2920 4340 1163
Size: 573 386 289 297 191 21 1420 4897
Fragments arranged by size:
4897 1420 573 386 297 289 191 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5236 5524 6285 7313 1540
Size: 275 186 3235 288 761 1028 2301
Fragments arranged by size:
3235 2301 1028 761 288 275 186
BsiHKAI G_wGCw'C
Cuts at: 3209 3243 3465 5091 6135 6220 7291 3209
Size: 34 222 1626 1044 85 1071 3992
Fragments arranged by size:
3992 1626 1071 1044 222 85 34
BsmI GAATG_Cn'
Cuts at: 5585 5678 5585
Size: 93 7981
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2213 2358 2604 2747 3209 3243
Size: 399 411 12 145 246 143 462 34
Cuts at: 3243 3465 3863 4051 4243 4404 5091 5135 5161
Size: 222 398 188 192 161 687 44 26
Cuts at: 5161 5360 5393 6135 6220 7291 1391
Size: 199 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 462 411 399 398
246 222 199 192 188 161 145 143
85 44 34 33 26 12
BspEI T'CCGG_A
Cuts at: 2235 7737 2235
Size: 5502 2572
BspHI T'CATG_A
Cuts at: 3225 5963 7693 3225
Size: 2738 1730 3606
Fragments arranged by size:
3606 2738 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2383 2680 3021 4597 5135 5429 844
Size: 1273 266 297 341 1576 538 294 3489
Fragments arranged by size:
3489 1576 1273 538 341 297 294 266
BsrBI CCG'CTC
Cuts at: 1394 1823 3040 4233 4930 5022 5961 1394
Size: 429 1217 1193 697 92 939 3507
Fragments arranged by size:
3507 1217 1193 939 697 429 92
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 4293 4768 4815 6569 6743 65
Size: 1183 847 2198 475 47 1754 174 1396
Fragments arranged by size:
2198 1754 1396 1183 847 475 174 47
BsrFI r'CCGG_y
Cuts at: 1700 1810 2619 3960 6718 6905 1700
Size: 110 809 1341 2758 187 2869
Fragments arranged by size:
2869 2758 1341 809 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8074
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8074
BssSI C'ACGA_G
Cuts at: 6134 7146 6134
Size: 1012 7062
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8074
BstBI TT'CG_AA
Cuts at: 2007 6878 2007
Size: 4871 3203
BstEII G'GTnAC_C
Cuts at: 1184 5245 1184
Size: 4061 4013
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 3400 3535 3751 4729 2650
Size: 750 135 216 978 5995
Fragments arranged by size:
5995 978 750 216 135
BstYI r'GATC_y
Cuts at: 1972 3129 3294 3988 4018 4119 5404 5771 6156
Size: 1157 165 694 30 101 1285 367 385
Cuts at: 6156 6173 7625 7711 7723 8069 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1285 1157 694 385 367 346
165 101 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3284 3882 4197 4984 3284
Size: 598 315 787 6374
Fragments arranged by size:
6374 787 598 315
BtgI C'CryG_G
Cuts at: 513 1065 2113 2860 2986 3075 3622 3750 4477
Size: 552 1048 747 126 89 547 128 727
Cuts at: 4477 5250 513
Size: 773 3337
Fragments arranged by size:
3337 1048 773 747 727 552 547 128
126 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2749 4882 487
Size: 683 56 1523 2133 3679
Fragments arranged by size:
3679 2133 1523 683 56
BtsI GCAGTG_nn'
Cuts at: 2286 2691 3207 3277 3355 3463 3581 5031 5590
Size: 405 516 70 78 108 118 1450 559
Cuts at: 5590 6384 6404 2286
Size: 794 20 3956
Fragments arranged by size:
3956 1450 794 559 516 405 118 108
78 70 20
ClaI AT'CG_AT
Cuts at: 1554 3918 1554
Size: 2364 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4429 4464 529
Size: 35 3865 35 4139
Fragments arranged by size:
4139 3865 35 35
DraI TTT'AAA
Cuts at: 5378 5378
Size: 8074
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7081 809
Size: 507 5765 1802
Fragments arranged by size:
5765 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 3525 3556
Size: 54 1425 50 275 18 591 1104 31
Cuts at: 3556 3720 4338 4380 5521 6410 8039 8
Size: 164 618 42 1141 889 1629 43
Fragments arranged by size:
1629 1425 1141 1104 889 618 591 275
164 54 50 43 42 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 5521 1537
Size: 275 3709 4090
Fragments arranged by size:
4090 3709 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6004 1353
Size: 4651 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 3809 6661 7035 7181 387
Size: 1064 138 1032 1188 2852 374 146 1280
Fragments arranged by size:
2852 1280 1188 1064 1032 374 146 138
EcoICRI GAG'CTC
Cuts at: 3241 3241
Size: 8074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 4868 5312 6137 6734 7521 7789
Size: 393 818 2418 444 825 597 787 268
Cuts at: 7789 1239
Size: 1524
Fragments arranged by size:
2418 1524 825 818 787 597 444 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 5261 5261
Size: 8074
EcoO109I rG'GnC_Cy
Cuts at: 2198 4201 4430 5227 5356 5357 2198
Size: 2003 229 797 129 1 4915
Fragments arranged by size:
4915 2003 797 229 129 1
EcoRI G'AATT_C
Cuts at: 5383 5761 5383
Size: 378 7696
FspI TGC'GCA
Cuts at: 3213 6580 3213
Size: 3367 4707
HaeII r_GCGC'y
Cuts at: 1678 2113 2345 2383 2437 2703 2968 3687 4589
Size: 435 232 38 54 266 265 719 902
Cuts at: 4589 4759 7221 1678
Size: 170 2462 2531
Fragments arranged by size:
2531 2462 902 719 435 266 265 232
170 54 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3491 3523 3973 4005 5292
Size: 32 135 32 1919 32 450 32 1287
Cuts at: 5292 5324 6715 6747 6789 6821 1373
Size: 32 1391 32 42 32 2626
Fragments arranged by size:
2626 1919 1391 1287 450 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4617 5418 5664 669
Size: 988 281 2679 801 246 3079
Fragments arranged by size:
3079 2679 988 801 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8074
HpaI GTT'AAC
Cuts at: 5664 5664
Size: 8074
KasI G'GCGC_C
Cuts at: 2109 2341 2379 3683 2109
Size: 232 38 1304 6500
Fragments arranged by size:
6500 1304 232 38
KpnI G_GTAC'C
Cuts at: 5399 5399
Size: 8074
MfeI C'AATT_G
Cuts at: 5673 5673
Size: 8074
MluI A'CGCG_T
Cuts at: 3639 7950 3639
Size: 4311 3763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7188 7372 1305
Size: 5883 184 2007
Fragments arranged by size:
5883 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3527 3722 4340 4382 10
Size: 54 1425 343 1695 195 618 42 3702
Fragments arranged by size:
3702 1695 1425 618 343 195 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2609 2648 2789 3080 3150 3202
Size: 648 1347 96 39 141 291 70 52
Cuts at: 3202 3360 3437 4109 4973 5082 6032 6391 6550
Size: 158 77 672 864 109 950 359 159
Cuts at: 6550 518
Size: 2042
Fragments arranged by size:
2042 1347 950 864 672 648 359 291
159 158 141 109 96 77 70 52
39
MspA1I CmG'CkG
Cuts at: 1606 2321 2446 2513 2811 2988 3453 3733 3940
Size: 715 125 67 298 177 465 280 207
Cuts at: 3940 4526 4942 5290 5490 6167 7315 7560 1606
Size: 586 416 348 200 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 586 465 416 348
298 280 245 207 200 177 125 67
NarI GG'CG_CC
Cuts at: 2110 2342 2380 3684 2110
Size: 232 38 1304 6500
Fragments arranged by size:
6500 1304 232 38
NcoI C'CATG_G
Cuts at: 513 1065 3075 3750 4477 513
Size: 552 2010 675 727 4110
Fragments arranged by size:
4110 2010 727 675 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8074
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8074
NotI GC'GGCC_GC
Cuts at: 5521 5521
Size: 8074
NspI r_CATG'y
Cuts at: 1132 1229 2794 3436 4306 4324 4601 5432 6977
Size: 97 1565 642 870 18 277 831 1545
Cuts at: 6977 1132
Size: 2229
Fragments arranged by size:
2229 1565 1545 870 831 642 277 97
18
PasI CC'CwG_GG
Cuts at: 1348 2287 1348
Size: 939 7135
PciI A'CATG_T
Cuts at: 4302 6973 4302
Size: 2671 5403
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 3623 4483 4744 1108
Size: 920 1595 860 261 4438
Fragments arranged by size:
4438 1595 920 860 261
PfoI T'CCnGG_A
Cuts at: 2163 3794 4007 2163
Size: 1631 213 6230
Fragments arranged by size:
6230 1631 213
PmeI GTTT'AAAC
Cuts at: 5378 5378
Size: 8074
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 6143 6175 7656 7688 4916
Size: 32 1195 32 1481 32 5302
Fragments arranged by size:
5302 1481 1195 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4430 5227 4430
Size: 797 7277
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8074
PsiI TTA'TAA
Cuts at: 5644 5644
Size: 8074
PspOMI G'GGCC_C
Cuts at: 2197 5131 5356 2197
Size: 2934 225 4915
Fragments arranged by size:
4915 2934 225
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 8042
PstI C_TGCA'G
Cuts at: 830 3018 3588 3732 4531 5426 830
Size: 2188 570 144 799 895 3478
Fragments arranged by size:
3478 2188 895 799 570 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8074
PvuII CAG'CTG
Cuts at: 3940 4526 4942 5290 3940
Size: 586 416 348 6724
Fragments arranged by size:
6724 586 416 348
SacI G_AGCT'C
Cuts at: 3243 3243
Size: 8074
SacII CC_GC'GG
Cuts at: 2989 2989
Size: 8074
SalI G'TCGA_C
Cuts at: 1936 4615 5416 1936
Size: 2679 801 4594
Fragments arranged by size:
4594 2679 801
SanDI GG'GwC_CC
Cuts at: 4430 5227 4430
Size: 797 7277
SbfI CC_TGCA'GG
Cuts at: 5426 5426
Size: 8074
ScaI AGT'ACT
Cuts at: 4004 6322 8034 4004
Size: 2318 1712 4044
Fragments arranged by size:
4044 2318 1712
SexAI A'CCwGG_T
Cuts at: 5262 5262
Size: 8074
SfcI C'TryA_G
Cuts at: 826 1045 3014 3584 3728 4527 4646 4682 5422
Size: 219 1969 570 144 799 119 36 740
Cuts at: 5422 6557 7238 7429 826
Size: 1135 681 191 1471
Fragments arranged by size:
1969 1471 1135 799 740 681 570 219
191 144 119 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 3555 3719 3555
Size: 164 7910
SfoI GGC'GCC
Cuts at: 2111 2343 2381 3685 2111
Size: 232 38 1304 6500
Fragments arranged by size:
6500 1304 232 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8074
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8074
SmaI CCC'GGG
Cuts at: 1872 3341 4751 5401 1872
Size: 1469 1410 650 4545
Fragments arranged by size:
4545 1469 1410 650
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3647 3992 6178 7079 7341 820
Size: 197 926 1704 345 2186 901 262 1553
Fragments arranged by size:
2186 1704 1553 926 901 345 262 197
SnaBI TAC'GTA
Cuts at: 493 5371 5865 493
Size: 4878 494 2702
Fragments arranged by size:
4878 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8074
SphI G_CATG'C
Cuts at: 1132 2794 4601 5432 1132
Size: 1662 1807 831 3774
Fragments arranged by size:
3774 1807 1662 831
SrfI GCCC'GGGC
Cuts at: 3341 3341
Size: 8074
SspI AAT'ATT
Cuts at: 5 52 5998 5
Size: 47 5946 2081
Fragments arranged by size:
5946 2081 47
StuI AGG'CCT
Cuts at: 1517 2972 3815 1517
Size: 1455 843 5776
Fragments arranged by size:
5776 1455 843
StyI C'CwwG_G
Cuts at: 513 1065 3075 3475 3750 3801 3810 4477 4722
Size: 552 2010 400 275 51 9 667 245
Cuts at: 4722 5277 5513 513
Size: 555 236 3074
Fragments arranged by size:
3074 2010 667 555 552 400 275 245
236 51 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2638 3878 5250 6101 6118 6271
Size: 1255 614 444 1240 1372 851 17 153
Cuts at: 6271 6456 6901 325
Size: 185 445 1498
Fragments arranged by size:
1498 1372 1255 1240 851 614 445 444
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4002 4173 4305
Size: 274 80 33 51 1443 2025 171 132
Cuts at: 4305 6320 8032 96
Size: 2015 1712 138
Fragments arranged by size:
2025 2015 1712 1443 274 171 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2929 2961 8034 8066 2630
Size: 32 267 32 5073 32 2638
Fragments arranged by size:
5073 2638 267 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3152 1316
Size: 1836 6238
XbaI T'CTAG_A
Cuts at: 5410 5410
Size: 8074
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5059 5114 5059
Size: 55 8019
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8074
XmaI C'CCGG_G
Cuts at: 1870 3339 4749 5399 1870
Size: 1469 1410 650 4545
Fragments arranged by size:
4545 1469 1410 650
XmnI GAAnn'nnTTC
Cuts at: 2411 6203 2411
Size: 3792 4282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5882 276
Size: 53 83 186 936 4348 2468
Fragments arranged by size:
4348 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SapI SwaI