Restriction map for
pFN21ASDA0396
(Circular) (Six-base) MAPSORT of: pFN21ASDA0396.seq Check: 8847 from: 1 to: 6610
pFN21ASDA0396 6610 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2498 2498
Size: 6610
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3540 4420 278
Size: 53 83 186 936 2004 880 2468
Fragments arranged by size:
2468 2004 936 880 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2046 3953 1937
Size: 109 1907 4594
Fragments arranged by size:
4594 1907 109
Acc65I G'GTAC_C
Cuts at: 3931 3931
Size: 6610
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 4673 6057 6325 1632
Size: 996 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 996 268
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 6610
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 5509 6486 1203
Size: 4306 977 1327
Fragments arranged by size:
4306 1327 977
AgeI A'CCGG_T
Cuts at: 2100 5441 2100
Size: 3341 3269
AhdI GACnn_n'nnGTC
Cuts at: 5339 5339
Size: 6610
AleI CACnn'nnGTG
Cuts at: 1166 2525 1166
Size: 1359 5251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6610
AlwNI CAG_nnn'CTG
Cuts at: 2962 3314 4353 5925 2962
Size: 352 1039 1572 3647
Fragments arranged by size:
3647 1572 1039 352
ApaLI G'TGCA_C
Cuts at: 4667 5823 4667
Size: 1156 5454
ApoI r'AATT_y
Cuts at: 1493 3098 3919 4145 4297 6406 1493
Size: 1605 821 226 152 2109 1697
Fragments arranged by size:
2109 1697 1605 821 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 3828 3935 1870
Size: 73 1885 107 4545
Fragments arranged by size:
4545 1885 107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3756 3789 3756
Size: 33 6577
BamHI G'GATC_C
Cuts at: 3940 4307 3940
Size: 367 6243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2285 2792 3931 5386
Size: 325 217 627 465 33 507 1139 1455
Cuts at: 5386 618
Size: 1842
Fragments arranged by size:
1842 1455 1139 627 507 465 325 217
33
BanII G_rGCy'C
Cuts at: 1391 3715 3929 1391
Size: 2324 214 4072
Fragments arranged by size:
4072 2324 214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5639
BbvCI CC'TCA_GC
Cuts at: 3591 3591
Size: 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4801 4835 1903
Size: 34 2864 34 3678
Fragments arranged by size:
3678 2864 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 5221 136
Size: 107 122 71 631 605 365 3184 1525
Fragments arranged by size:
3184 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 3432 6605 3432
Size: 3173 3437
BlpI GC'TnA_GC
Cuts at: 4027 4027
Size: 6610
Bme1580I G_kGCm'C
Cuts at: 1790 2619 3628 4671 5827 1790
Size: 829 1009 1043 1156 2573
Fragments arranged by size:
2573 1156 1043 1009 829
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3777 4341 5299
Size: 416 53 83 399 24 2360 564 958
Cuts at: 5299 442
Size: 1753
Fragments arranged by size:
2360 1753 958 564 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2984 3495 5270 1239
Size: 1092 653 511 1775 2579
Fragments arranged by size:
2579 1775 1092 653 511
Bpu10I CC'TnA_GC
Cuts at: 3591 3591
Size: 6610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3174 3797 4735 5600 5898 3174
Size: 623 938 865 298 3886
Fragments arranged by size:
3886 938 865 623 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5273 882
Size: 617 3774 2219
Fragments arranged by size:
3774 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3907 4401 493
Size: 711 2703 494 2702
Fragments arranged by size:
2703 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2684 3537 4417 4799
Size: 53 83 186 936 1151 853 880 382
Cuts at: 4799 5434 275
Size: 635 1451
Fragments arranged by size:
1451 1151 936 880 853 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2100 5043 5441 5715 5862 6273 2100
Size: 2943 398 274 147 411 2437
Fragments arranged by size:
2943 2437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2346 2376 1178
Size: 30 165 30 943 30 5412
Fragments arranged by size:
5412 943 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3087 1186
Size: 1901 4709
BseYI C'CCAG_C
Cuts at: 2612 5813 6309 2612
Size: 3201 496 2913
Fragments arranged by size:
3201 2913 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2325 3119 3418 1163
Size: 573 519 70 794 299 4355
Fragments arranged by size:
4355 794 573 519 299 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 4060 4821 5849 1540
Size: 275 186 757 1302 761 1028 2301
Fragments arranged by size:
2301 1302 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 3715 4671 4756 5827 3715
Size: 956 85 1071 4498
Fragments arranged by size:
4498 1071 956 85
BsmI GAATG_Cn'
Cuts at: 3604 4121 4214 3604
Size: 517 93 6000
Fragments arranged by size:
6000 517 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2619 3628 3715 3929 4671 4756 5827
Size: 399 829 1009 87 214 742 85 1071
Cuts at: 5827 1391
Size: 2174
Fragments arranged by size:
2174 1071 1009 829 742 399 214 87
85
BspEI T'CCGG_A
Cuts at: 6273 6273
Size: 6610
BspHI T'CATG_A
Cuts at: 4499 6229 4499
Size: 1730 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2498 3965 844
Size: 1654 1467 3489
Fragments arranged by size:
3489 1654 1467
BsrBI CCG'CTC
Cuts at: 1394 1823 4497 1394
Size: 429 2674 3507
Fragments arranged by size:
3507 2674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 2466 2591 5105 5279 65
Size: 1183 946 272 125 2514 174 1396
Fragments arranged by size:
2514 1396 1183 946 272 174 125
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 5254 5441 1700
Size: 110 290 3154 187 2869
Fragments arranged by size:
3154 2869 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 3465 4670 5682 3465
Size: 1205 1012 4393
Fragments arranged by size:
4393 1205 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6610
BstBI TT'CG_AA
Cuts at: 2007 5414 2007
Size: 3407 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6610
BstXI CCAn_nnnn'nTGG
Cuts at: 2927 2927
Size: 6610
BstYI r'GATC_y
Cuts at: 1972 3172 3432 3867 3940 4307 4692 4709 6161
Size: 1200 260 435 73 367 385 17 1452
Cuts at: 6161 6247 6259 6605 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1200 435 385 367 346 260
86 73 17 12
BstZ17I GTA'TAC
Cuts at: 2047 2047
Size: 6610
Bsu36I CC'TnA_GG
Cuts at: 2955 2955
Size: 6610
BtgI C'CryG_G
Cuts at: 513 1065 2022 2350 2674 3603 513
Size: 552 957 328 324 929 3520
Fragments arranged by size:
3520 957 929 552 328 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 487
Size: 683 56 935 4936
Fragments arranged by size:
4936 935 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 3113 3268 4126 4920 4940 2289
Size: 824 155 858 794 20 3959
Fragments arranged by size:
3959 858 824 794 155 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DraIII CAC_nnn'GTG
Cuts at: 2579 2579
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2273 5617 809
Size: 507 957 3344 1802
Fragments arranged by size:
3344 1802 957 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2082 2671
Size: 54 1425 50 275 18 225 27 589
Cuts at: 2671 4057 4946 6575 8
Size: 1386 889 1629 43
Fragments arranged by size:
1629 1425 1386 889 589 275 225 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 4057 1537
Size: 275 2245 4090
Fragments arranged by size:
4090 2245 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2997 3879 4540 1353
Size: 1644 882 661 3423
Fragments arranged by size:
3423 1644 882 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5197 5571 5717 387
Size: 1064 138 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3713 3713
Size: 6610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2628 2984 3495 4673 5270 6057
Size: 393 699 297 356 511 1178 597 787
Cuts at: 6057 6325 1239
Size: 268 1524
Fragments arranged by size:
1524 1178 787 699 597 511 393 356
297 268
EcoO109I rG'GnC_Cy
Cuts at: 2029 3232 2029
Size: 1203 5407
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2924 2924
Size: 6610
FspI TGC'GCA
Cuts at: 5116 5116
Size: 6610
HaeII r_GCGC'y
Cuts at: 1678 2161 5757 1678
Size: 483 3596 2531
Fragments arranged by size:
3596 2531 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5251 5283 5325 5357 1373
Size: 32 135 32 3679 32 42 32 2626
Fragments arranged by size:
3679 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3499 3954 4200 669
Size: 988 281 1561 455 246 3079
Fragments arranged by size:
3079 1561 988 455 281 246
HindIII A'AGCT_T
Cuts at: 748 2775 748
Size: 2027 4583
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KpnI G_GTAC'C
Cuts at: 3935 3935
Size: 6610
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2747 3874 5724 5908 1305
Size: 1442 1127 1850 184 2007
Fragments arranged by size:
2007 1850 1442 1127 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 10
Size: 54 1425 343 841 3947
Fragments arranged by size:
3947 1425 841 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 2525 2925 3216 3531 3722 4568
Size: 648 1357 2 400 291 315 191 846
Cuts at: 4568 4927 5086 518
Size: 359 159 2042
Fragments arranged by size:
2042 1357 846 648 400 359 315 291
191 159 2
MspA1I CmG'CkG
Cuts at: 1606 2309 2606 4026 4703 5851 6096 1606
Size: 703 297 1420 677 1148 245 2120
Fragments arranged by size:
2120 1420 1148 703 677 297 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2350 2674 513
Size: 552 957 328 324 4449
Fragments arranged by size:
4449 957 552 328 324
NdeI CA'TA_TG
Cuts at: 387 2997 387
Size: 2610 4000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 2409 3968 5513 1132
Size: 97 1180 1559 1545 2229
Fragments arranged by size:
2229 1559 1545 1180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6610
PciI A'CATG_T
Cuts at: 5509 5509
Size: 6610
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6610
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2721 2753 4679 4711 6192 6224 2721
Size: 32 1926 32 1481 32 3107
Fragments arranged by size:
3107 1926 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PstI C_TGCA'G
Cuts at: 830 3545 3962 830
Size: 2715 417 3478
Fragments arranged by size:
3478 2715 417
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2309 2606 2309
Size: 297 6313
SacI G_AGCT'C
Cuts at: 3715 3715
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SapI GCTCTTCn'nnn_
Cuts at: 2997 2997
Size: 6610
SbfI CC_TGCA'GG
Cuts at: 3962 3962
Size: 6610
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SfcI C'TryA_G
Cuts at: 826 1045 2658 3541 3958 5093 5774 5965 826
Size: 219 1613 883 417 1135 681 191 1471
Fragments arranged by size:
1613 1471 1135 883 681 417 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 3937 1872
Size: 2065 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3189 3812 4714 5615 5877 820
Size: 197 926 1246 623 902 901 262 1553
Fragments arranged by size:
1553 1246 926 902 901 623 262 197
SnaBI TAC'GTA
Cuts at: 493 3907 4401 493
Size: 3414 494 2702
Fragments arranged by size:
3414 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 2409 3968 1132
Size: 1277 1559 3774
Fragments arranged by size:
3774 1559 1277
SspI AAT'ATT
Cuts at: 5 52 3043 3304 4534 5
Size: 47 2991 261 1230 2081
Fragments arranged by size:
2991 2081 1230 261 47
StuI AGG'CCT
Cuts at: 1517 3006 1517
Size: 1489 5121
StyI C'CwwG_G
Cuts at: 513 1065 2022 2350 2674 4049 513
Size: 552 957 328 324 1375 3074
Fragments arranged by size:
3074 1375 957 552 328 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2772 2780 3179 4637 4654 4807 4992
Size: 1255 1192 8 399 1458 17 153 185
Cuts at: 4992 5437 325
Size: 445 1498
Fragments arranged by size:
1498 1458 1255 1192 445 399 185 153
17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3645 3761 4856
Size: 274 80 33 51 1443 1668 116 1095
Cuts at: 4856 6568 96
Size: 1712 138
Fragments arranged by size:
1712 1668 1443 1095 274 138 116 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2346 2378 3016 3048 6570 6602 2346
Size: 32 638 32 3522 32 2354
Fragments arranged by size:
3522 2354 638 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6610
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 6610
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6610
XmaI C'CCGG_G
Cuts at: 1870 3935 1870
Size: 2065 4545
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3538 4418 276
Size: 53 83 186 936 2004 880 2468
Fragments arranged by size:
2468 2004 936 880 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI