Restriction map for
pFN21ASDA0388
(Circular) (Six-base) MAPSORT of: pFN21ASDA0388.seq Check: 3735 from: 1 to: 6970
pFN21ASDA0388 6970 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3632 3873 3632
Size: 241 6729
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4260 4780 278
Size: 53 83 186 936 2724 520 2468
Fragments arranged by size:
2724 2468 936 520 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4313 1937
Size: 2376 4594
Acc65I G'GTAC_C
Cuts at: 4291 4291
Size: 6970
AclI AA'CG_TT
Cuts at: 1563 5097 5470 1563
Size: 3534 373 3063
Fragments arranged by size:
3534 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2716 3207 3354 3479 3569 5033 6417
Size: 470 614 491 147 125 90 1464 1384
Cuts at: 6417 6685 1632
Size: 268 1917
Fragments arranged by size:
1917 1464 1384 614 491 470 268 147
125 90
AflII C'TTAA_G
Cuts at: 820 1017 3854 820
Size: 197 2837 3936
Fragments arranged by size:
3936 2837 197
AflIII A'CryG_T
Cuts at: 1203 4008 5869 6846 1203
Size: 2805 1861 977 1327
Fragments arranged by size:
2805 1861 1327 977
AgeI A'CCGG_T
Cuts at: 5801 5801
Size: 6970
AhdI GACnn_n'nnGTC
Cuts at: 5699 5699
Size: 6970
AleI CACnn'nnGTG
Cuts at: 1166 3572 1166
Size: 2406 4564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6970
AlwNI CAG_nnn'CTG
Cuts at: 3155 3624 4713 6285 3155
Size: 469 1089 1572 3840
Fragments arranged by size:
3840 1572 1089 469
ApaLI G'TGCA_C
Cuts at: 2839 5027 6183 2839
Size: 2188 1156 3626
Fragments arranged by size:
3626 2188 1156
ApoI r'AATT_y
Cuts at: 1493 2155 3646 3932 4074 4279 4505 4657 6766
Size: 662 1491 286 142 205 226 152 2109
Cuts at: 6766 1493
Size: 1697
Fragments arranged by size:
2109 1697 1491 662 286 226 205 152
142
AseI AT'TA_AT
Cuts at: 160 1032 5524 160
Size: 872 4492 1606
Fragments arranged by size:
4492 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 4295 1870
Size: 73 347 2005 4545
Fragments arranged by size:
4545 2005 347 73
BamHI G'GATC_C
Cuts at: 2522 4300 4667 2522
Size: 1778 367 4825
Fragments arranged by size:
4825 1778 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3114 3362 3899 4291 5746
Size: 325 217 627 1327 248 537 392 1455
Cuts at: 5746 618
Size: 1842
Fragments arranged by size:
1842 1455 1327 627 537 392 325 248
217
BanII G_rGCy'C
Cuts at: 1391 3480 3960 4191 4289 1391
Size: 2089 480 231 98 4072
Fragments arranged by size:
4072 2089 480 231 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3346 928
Size: 971 1447 4552
Fragments arranged by size:
4552 1447 971
BbvCI CC'TCA_GC
Cuts at: 3148 3148
Size: 6970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5161 5195 1903
Size: 34 3224 34 3678
Fragments arranged by size:
3678 3224 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4862 6072 4862
Size: 1210 5760
BclI T'GATC_A
Cuts at: 1548 2472 1548
Size: 924 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4166 5581 136
Size: 107 122 71 631 605 2494 1415 1525
Fragments arranged by size:
2494 1525 1415 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2644 6965 2644
Size: 4321 2649
BlpI GC'TnA_GC
Cuts at: 4387 4387
Size: 6970
Bme1580I G_kGCm'C
Cuts at: 1790 2604 2843 3902 5031 6187 1790
Size: 814 239 1059 1129 1156 2573
Fragments arranged by size:
2573 1156 1129 1059 814 239
BmgBI CAC'GTC
Cuts at: 1318 3414 1318
Size: 2096 4874
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3217 3336 3712
Size: 416 53 83 399 24 1800 119 376
Cuts at: 3712 3814 4701 5659 442
Size: 102 887 958 1753
Fragments arranged by size:
1800 1753 958 887 416 399 376 119
102 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3979 3250
Size: 729 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2573 3199 5630 1239
Size: 1334 626 2431 2579
Fragments arranged by size:
2579 2431 1334 626
Bpu10I CC'TnA_GC
Cuts at: 3148 3148
Size: 6970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4211 5095 5960 6258 4211
Size: 884 865 298 4923
Fragments arranged by size:
4923 884 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4131 4244 5633 882
Size: 617 2632 113 1389 2219
Fragments arranged by size:
2632 2219 1389 617 113
BsaAI yAC'GTr
Cuts at: 493 1204 4234 4267 4761 493
Size: 711 3030 33 494 2702
Fragments arranged by size:
3030 2702 711 494 33
BsaBI GATnn'nnATC
Cuts at: 4151 6964 4151
Size: 2813 4157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4257 4777 5159 5794
Size: 53 83 186 936 2724 520 382 635
Cuts at: 5794 275
Size: 1451
Fragments arranged by size:
2724 1451 936 635 520 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5403 5801 6075 6222 6633 5403
Size: 398 274 147 411 5740
Fragments arranged by size:
5740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2421 2451 3068 3098 3197
Size: 30 165 30 1018 30 617 30 99
Cuts at: 3197 3227 1178
Size: 30 4921
Fragments arranged by size:
4921 1018 617 165 99 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2603 2606 1186
Size: 1294 123 3 5550
Fragments arranged by size:
5550 1294 123 3
BseYI C'CCAG_C
Cuts at: 6173 6669 6173
Size: 496 6474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 2897 3510 3941 1163
Size: 573 1143 18 613 431 4192
Fragments arranged by size:
4192 1143 613 573 431 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4420 5181 6209 1540
Size: 275 186 2419 761 1028 2301
Fragments arranged by size:
2419 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2843 3095 3480 3804 3960 4191 5031 5116 6187
Size: 252 385 324 156 231 840 85 1071
Cuts at: 6187 2843
Size: 3626
Fragments arranged by size:
3626 1071 840 385 324 252 231 156
85
BsmI GAATG_Cn'
Cuts at: 4481 4574 4481
Size: 93 6877
BsmBI CGTCTCn'nnnn_
Cuts at: 2393 2393
Size: 6970
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2604 2843 3095 3480 3804 3902 3960
Size: 399 814 239 252 385 324 98 58
Cuts at: 3960 4191 4289 5031 5116 6187 1391
Size: 231 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 814 742 399 385 324 252
239 231 98 98 85 58
BspEI T'CCGG_A
Cuts at: 6633 6633
Size: 6970
BspHI T'CATG_A
Cuts at: 3480 3636 4859 6589 3480
Size: 156 1223 1730 3861
Fragments arranged by size:
3861 1730 1223 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3412 3632 3873 4325 844
Size: 2568 220 241 452 3489
Fragments arranged by size:
3489 2568 452 241 220
BsrBI CCG'CTC
Cuts at: 1394 1823 3084 4857 1394
Size: 429 1261 1773 3507
Fragments arranged by size:
3507 1773 1261 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3208 3740 5465 5639 65
Size: 1183 1960 532 1725 174 1396
Fragments arranged by size:
1960 1725 1396 1183 532 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2919 5614 5801 1700
Size: 110 1109 2695 187 2869
Fragments arranged by size:
2869 2695 1109 187 110
BsrGI T'GTAC_A
Cuts at: 96 3576 96
Size: 3480 3490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6970
BssSI C'ACGA_G
Cuts at: 5030 6042 5030
Size: 1012 5958
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2159 2893 3030 3868 794
Size: 1365 734 137 838 3896
Fragments arranged by size:
3896 1365 838 734 137
BstBI TT'CG_AA
Cuts at: 2007 5774 2007
Size: 3767 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6970
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 4148 3400
Size: 748 6222
BstYI r'GATC_y
Cuts at: 1972 2184 2522 2644 4152 4300 4667 5052 5069
Size: 212 338 122 1508 148 367 385 17
Cuts at: 5069 6521 6607 6619 6965 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1508 1452 385 367 346 338 212
148 122 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2497 3358 513
Size: 552 957 475 861 4125
Fragments arranged by size:
4125 957 861 552 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6231
Fragments arranged by size:
6231 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 3759 4486 5280 5300 3529
Size: 230 727 794 20 5199
Fragments arranged by size:
5199 794 727 230 20
ClaI AT'CG_AT
Cuts at: 1554 2853 1554
Size: 1299 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6935
DraI TTT'AAA
Cuts at: 2932 4274 2932
Size: 1342 5628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5977 809
Size: 507 4661 1802
Fragments arranged by size:
4661 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3888 4417 5306
Size: 54 1425 50 275 18 2058 529 889
Cuts at: 5306 6935 8
Size: 1629 43
Fragments arranged by size:
2058 1629 1425 889 529 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4417 1537
Size: 275 2605 4090
Fragments arranged by size:
4090 2605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2502 3568 4028 4179 4196 4900 1353
Size: 1149 1066 460 151 17 704 3423
Fragments arranged by size:
3423 1149 1066 704 460 151 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5557 5931 6077 387
Size: 1064 138 3968 374 146 1280
Fragments arranged by size:
3968 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3478 3958 4189 3478
Size: 480 231 6259
Fragments arranged by size:
6259 480 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2573 2716 3199 3207 3354 3479
Size: 393 470 471 143 483 8 147 125
Cuts at: 3479 3569 5033 5630 6417 6685 1239
Size: 90 1464 597 787 268 1524
Fragments arranged by size:
1524 1464 787 597 483 471 470 393
268 147 143 125 90 8
EcoO109I rG'GnC_Cy
Cuts at: 3792 3792
Size: 6970
EcoRI G'AATT_C
Cuts at: 3932 4279 4657 3932
Size: 347 378 6245
Fragments arranged by size:
6245 378 347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3359 3442 3467 3359
Size: 83 25 6862
Fragments arranged by size:
6862 83 25
FspI TGC'GCA
Cuts at: 5476 5476
Size: 6970
HaeII r_GCGC'y
Cuts at: 1678 2926 6117 1678
Size: 1248 3191 2531
Fragments arranged by size:
3191 2531 1248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3197 3229 5611 5643 5685
Size: 32 135 32 1625 32 2382 32 42
Cuts at: 5685 5717 1373
Size: 32 2626
Fragments arranged by size:
2626 2382 1625 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4314 4560 669
Size: 988 281 2376 246 3079
Fragments arranged by size:
3079 2376 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2207 748
Size: 1459 5511
HpaI GTT'AAC
Cuts at: 4560 4560
Size: 6970
KpnI G_GTAC'C
Cuts at: 4295 4295
Size: 6970
MfeI C'AATT_G
Cuts at: 2739 4569 2739
Size: 1830 5140
MluI A'CGCG_T
Cuts at: 6846 6846
Size: 6970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 2398 6084 6268 1305
Size: 937 156 3686 184 2007
Fragments arranged by size:
3686 2007 937 184 156
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5148
Fragments arranged by size:
5148 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3572 4928 5287 5446 518
Size: 648 2406 1356 359 159 2042
Fragments arranged by size:
2406 2042 1356 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2881 3547 4386 5063 6211 6456 1606
Size: 1275 666 839 677 1148 245 2120
Fragments arranged by size:
2120 1275 1148 839 677 666 245
NaeI GCC'GGC
Cuts at: 2921 2921
Size: 6970
NcoI C'CATG_G
Cuts at: 513 1065 2022 2497 3358 513
Size: 552 957 475 861 4125
Fragments arranged by size:
4125 957 861 552 475
NdeI CA'TA_TG
Cuts at: 387 2310 387
Size: 1923 5047
NgoMIV G'CCGG_C
Cuts at: 2919 2919
Size: 6970
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6970
NotI GC'GGCC_GC
Cuts at: 4417 4417
Size: 6970
NspI r_CATG'y
Cuts at: 1132 1229 2095 2678 4012 4328 5873 1132
Size: 97 866 583 1334 316 1545 2229
Fragments arranged by size:
2229 1545 1334 866 583 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6970
PciI A'CATG_T
Cuts at: 4008 5869 4008
Size: 1861 5109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6970
PmeI GTTT'AAAC
Cuts at: 4274 4274
Size: 6970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5039 5071 6552 6584 5039
Size: 32 1481 32 5425
Fragments arranged by size:
5425 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3777 4256 1613
Size: 2164 479 4327
Fragments arranged by size:
4327 2164 479
PsiI TTA'TAA
Cuts at: 4540 4540
Size: 6970
PstI C_TGCA'G
Cuts at: 830 3536 3839 4322 830
Size: 2706 303 483 3478
Fragments arranged by size:
3478 2706 483 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6970
PvuII CAG'CTG
Cuts at: 3547 3547
Size: 6970
SacI G_AGCT'C
Cuts at: 3480 3960 4191 3480
Size: 480 231 6259
Fragments arranged by size:
6259 480 231
SalI G'TCGA_C
Cuts at: 1936 4312 1936
Size: 2376 4594
SapI GCTCTTCn'nnn_
Cuts at: 4179 4196 4179
Size: 17 6953
SbfI CC_TGCA'GG
Cuts at: 4322 4322
Size: 6970
ScaI AGT'ACT
Cuts at: 2579 5218 6930 2579
Size: 2639 1712 2619
Fragments arranged by size:
2639 2619 1712
SfcI C'TryA_G
Cuts at: 826 1045 3532 3835 4318 5453 6134 6325 826
Size: 219 2487 303 483 1135 681 191 1471
Fragments arranged by size:
2487 1471 1135 681 483 303 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6970
SmaI CCC'GGG
Cuts at: 1872 2292 4297 1872
Size: 420 2005 4545
Fragments arranged by size:
4545 2005 420
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3854 4226 5074 5975 6237 820
Size: 197 926 1911 372 848 901 262 1553
Fragments arranged by size:
1911 1553 926 901 848 372 262 197
SnaBI TAC'GTA
Cuts at: 493 4267 4761 493
Size: 3774 494 2702
Fragments arranged by size:
3774 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6970
SphI G_CATG'C
Cuts at: 1132 4328 1132
Size: 3196 3774
SspI AAT'ATT
Cuts at: 5 52 2416 4894 5
Size: 47 2364 2478 2081
Fragments arranged by size:
2478 2364 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6970
StyI C'CwwG_G
Cuts at: 513 1065 2022 2497 3358 4409 513
Size: 552 957 475 861 1051 3074
Fragments arranged by size:
3074 1051 957 861 552 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 2860 3615 4268 4997 5014 5167
Size: 1255 540 740 755 653 729 17 153
Cuts at: 5167 5352 5797 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 755 740 729 653 540 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 3576 5216
Size: 274 80 33 51 1443 600 999 1640
Cuts at: 5216 6928 96
Size: 1712 138
Fragments arranged by size:
1712 1640 1443 999 600 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 6930 6962 2421
Size: 32 4477 32 2429
Fragments arranged by size:
4477 2429 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6970
XbaI T'CTAG_A
Cuts at: 4306 4306
Size: 6970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4123 4123
Size: 6970
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6970
XmaI C'CCGG_G
Cuts at: 1870 2290 4295 1870
Size: 420 2005 4545
Fragments arranged by size:
4545 2005 420
XmnI GAAnn'nnTTC
Cuts at: 2711 3692 3932 5099 2711
Size: 981 240 1167 4582
Fragments arranged by size:
4582 1167 981 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4258 4778 276
Size: 53 83 186 936 2724 520 2468
Fragments arranged by size:
2724 2468 936 520 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI