Restriction map for
pFN21ASDA0387
(Circular) (Six-base) MAPSORT of: pFN21ASDA0387.seq Check: 3760 from: 1 to: 7843
pFN21ASDA0387 7843 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2562 3694 2562
Size: 1132 6711
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3690 5653 278
Size: 53 83 186 936 2154 1963 2468
Fragments arranged by size:
2468 2154 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5186 1937
Size: 3249 4594
Acc65I G'GTAC_C
Cuts at: 5164 5164
Size: 7843
AclI AA'CG_TT
Cuts at: 1563 5970 6343 1563
Size: 4407 373 3063
Fragments arranged by size:
4407 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 3044 3384 4052 4328 4478 4787 5906
Size: 840 572 340 668 276 150 309 1119
Cuts at: 5906 7290 7558 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1119 840 668 572 340 309
276 268 150
AfeI AGC'GCT
Cuts at: 4344 4344
Size: 7843
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7646
AflIII A'CryG_T
Cuts at: 1203 2685 4149 6742 7719 1203
Size: 1482 1464 2593 977 1327
Fragments arranged by size:
2593 1482 1464 1327 977
AgeI A'CCGG_T
Cuts at: 6674 6674
Size: 7843
AhdI GACnn_n'nnGTC
Cuts at: 3310 6572 3310
Size: 3262 4581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7843
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4703 4735 4703
Size: 32 7811
AlwNI CAG_nnn'CTG
Cuts at: 2185 2335 4096 5586 7158 2185
Size: 150 1761 1490 1572 2870
Fragments arranged by size:
2870 1761 1572 1490 150
ApaI G_GGCC'C
Cuts at: 2715 3531 4145 2715
Size: 816 614 6413
Fragments arranged by size:
6413 816 614
ApaLI G'TGCA_C
Cuts at: 2901 5900 7056 2901
Size: 2999 1156 3688
Fragments arranged by size:
3688 2999 1156
ApoI r'AATT_y
Cuts at: 1493 3855 5152 5378 5530 7639 1493
Size: 2362 1297 226 152 2109 1697
Fragments arranged by size:
2362 2109 1697 1297 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6397 160
Size: 872 5365 1606
Fragments arranged by size:
5365 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7843
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2708 2864 3135 3287 3485 3659
Size: 73 270 495 156 271 152 198 174
Cuts at: 3659 4045 4446 4522 5168 1870
Size: 386 401 76 646 4545
Fragments arranged by size:
4545 646 495 401 386 271 270 198
174 156 152 76 73
BamHI G'GATC_C
Cuts at: 5173 5540 5173
Size: 367 7476
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2271 2932 3706 4949 5164
Size: 325 217 627 484 661 774 1243 215
Cuts at: 5164 6619 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1243 774 661 627 484 325
217 215
BanII G_rGCy'C
Cuts at: 1391 2715 2778 3334 3486 3510 3531 4145 4368
Size: 1324 63 556 152 24 21 614 223
Cuts at: 4368 5162 1391
Size: 794 4072
Fragments arranged by size:
4072 1324 794 614 556 223 152 63
24 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 3381 3390 3927 928
Size: 971 1086 396 9 537 4844
Fragments arranged by size:
4844 1086 971 537 396 9
BbvCI CC'TCA_GC
Cuts at: 2637 3048 3575 5100 2637
Size: 411 527 1525 5380
Fragments arranged by size:
5380 1525 527 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3703 3737 4049 4083 6034 6068 1903
Size: 34 1766 34 312 34 1951 34 3678
Fragments arranged by size:
3678 1951 1766 312 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5735 6945 5735
Size: 1210 6633
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7843
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3066 4358 4571
Size: 107 122 71 631 605 1394 1292 213
Cuts at: 4571 4947 6454 136
Size: 376 1507 1525
Fragments arranged by size:
1525 1507 1394 1292 631 605 376 213
122 107 71
BglII A'GATC_T
Cuts at: 3454 7838 3454
Size: 4384 3459
BlpI GC'TnA_GC
Cuts at: 2777 3090 5260 2777
Size: 313 2170 5360
Fragments arranged by size:
5360 2170 313
Bme1580I G_kGCm'C
Cuts at: 1790 2715 2905 3531 3711 4145 4407 5904 7060
Size: 925 190 626 180 434 262 1497 1156
Cuts at: 7060 1790
Size: 2573
Fragments arranged by size:
2573 1497 1156 925 626 434 262 190
180
BmgBI CAC'GTC
Cuts at: 1318 2596 2688 3730 4152 1318
Size: 1278 92 1042 422 5009
Fragments arranged by size:
5009 1278 1042 422 92
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4171 5128 5574
Size: 416 53 83 399 24 2754 957 446
Cuts at: 5574 6532 442
Size: 958 1753
Fragments arranged by size:
2754 1753 958 957 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4503 1056
Size: 3447 4396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3859 4060 3859
Size: 201 7642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 2747 2900 3092 3192 3348 3854 4346
Size: 1346 162 153 192 100 156 506 492
Cuts at: 4346 5045 6503 1239
Size: 699 1458 2579
Fragments arranged by size:
2579 1458 1346 699 506 492 192 162
156 153 100
Bpu10I CC'TnA_GC
Cuts at: 2637 3048 3564 3575 5100 2637
Size: 411 516 11 1525 5380
Fragments arranged by size:
5380 1525 516 411 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2893 3875 4834 5053 5105 5968 6833 7131 2893
Size: 982 959 219 52 863 865 298 3605
Fragments arranged by size:
3605 982 959 865 863 298 219 52
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3318 3639 3958 4723 5038 6506 882
Size: 617 1819 321 319 765 315 1468 2219
Fragments arranged by size:
2219 1819 1468 765 617 321 319 315
BsaAI yAC'GTr
Cuts at: 493 1204 2376 5140 5634 493
Size: 711 1172 2764 494 2702
Fragments arranged by size:
2764 2702 1172 711 494
BsaBI GATnn'nnATC
Cuts at: 5009 7837 5009
Size: 2828 5015
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2446 2548 3687 3975
Size: 53 83 186 936 913 102 1139 288
Cuts at: 3975 4647 5650 6032 6667 275
Size: 672 1003 382 635 1451
Fragments arranged by size:
1451 1139 1003 936 913 672 635 382
288 186 102 83 53
BsaWI w'CCGG_w
Cuts at: 3866 6276 6674 6948 7095 7506 3866
Size: 2410 398 274 147 411 4203
Fragments arranged by size:
4203 2410 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2494 2524 2558 2588 2639
Size: 30 165 30 1091 30 34 30 51
Cuts at: 2639 2669 2689 2719 2873 2903 4319 4349 4703
Size: 30 20 30 154 30 1416 30 354
Cuts at: 4703 4733 1178
Size: 30 4288
Fragments arranged by size:
4288 1416 1091 354 165 154 51 34
30 30 30 30 30 30 30 30
30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2515 3422 3554 3894 4253 4409 4768
Size: 1045 284 907 132 340 359 156 359
Cuts at: 4768 4837 4848 5117 1186
Size: 69 11 269 3912
Fragments arranged by size:
3912 1045 907 359 359 340 284 269
156 132 69 11
BseYI C'CCAG_C
Cuts at: 2464 2584 2721 2854 3460 3710 4199 7046 7542
Size: 120 137 133 606 250 489 2847 496
Cuts at: 7542 2464
Size: 2765
Fragments arranged by size:
2847 2765 606 496 489 250 137 133
120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 3254 4089 4268 4956 1163
Size: 573 474 1044 835 179 688 4050
Fragments arranged by size:
4050 1044 835 688 573 474 179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5293 6054 7082 1540
Size: 275 186 3292 761 1028 2301
Fragments arranged by size:
3292 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 2778 2905 3007 3381 3510 3565 4728 5031
Size: 177 127 102 374 129 55 1163 303
Cuts at: 5031 5904 5989 7060 2601
Size: 873 85 1071 3384
Fragments arranged by size:
3384 1163 1071 873 374 303 177 129
127 102 85 55
BsmI GAATG_Cn'
Cuts at: 2919 3475 5354 5447 2919
Size: 556 1879 93 5315
Fragments arranged by size:
5315 1879 556 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4174 4781 4174
Size: 607 7236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2601 2715 2778 2905 3007 3334 3381
Size: 399 811 114 63 127 102 327 47
Cuts at: 3381 3486 3510 3531 3565 3711 4145 4368 4407
Size: 105 24 21 34 146 434 223 39
Cuts at: 4407 4728 5031 5162 5904 5989 7060 1391
Size: 321 303 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 434 399 327 321
303 223 146 131 127 114 105 102
85 63 47 39 34 24 21
BspEI T'CCGG_A
Cuts at: 7506 7506
Size: 7843
BspHI T'CATG_A
Cuts at: 5732 7462 5732
Size: 1730 6113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2562 3039 3694 4782 5198 844
Size: 1718 477 655 1088 416 3489
Fragments arranged by size:
3489 1718 1088 655 477 416
BsrBI CCG'CTC
Cuts at: 1394 1823 3155 4416 4602 4947 5730 1394
Size: 429 1332 1261 186 345 783 3507
Fragments arranged by size:
3507 1332 1261 783 429 345 186
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 4921 6338 6512 65
Size: 1183 1047 2626 1417 174 1396
Fragments arranged by size:
2626 1417 1396 1183 1047 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3043 4271 4320 6487 6674 1700
Size: 110 1233 1228 49 2167 187 2869
Fragments arranged by size:
2869 2167 1233 1228 187 110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7843
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7843
BssSI C'ACGA_G
Cuts at: 2729 5903 6915 2729
Size: 3174 1012 3657
Fragments arranged by size:
3657 3174 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 794
Size: 1541 6302
BstBI TT'CG_AA
Cuts at: 2007 6647 2007
Size: 4640 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7843
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3524 2029
Size: 1495 6348
BstYI r'GATC_y
Cuts at: 1972 3454 5173 5540 5925 5942 7394 7480 7492
Size: 1482 1719 367 385 17 1452 86 12
Cuts at: 7492 7838 1972
Size: 346 1977
Fragments arranged by size:
1977 1719 1482 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2441 2784 3906 4137 2441
Size: 343 1122 231 6147
Fragments arranged by size:
6147 1122 343 231
BtgI C'CryG_G
Cuts at: 513 1065 2022 2319 2744 3124 3205 3307 4569
Size: 552 957 297 425 380 81 102 1262
Cuts at: 4569 5094 513
Size: 525 3262
Fragments arranged by size:
3262 1262 957 552 525 425 380 297
102 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3012 3922 4525 487
Size: 683 56 1786 910 603 3805
Fragments arranged by size:
3805 1786 910 683 603 56
BtsI GCAGTG_nn'
Cuts at: 2045 2186 3756 4077 4661 4933 5359 6153 6173
Size: 141 1570 321 584 272 426 794 20
Cuts at: 6173 2045
Size: 3715
Fragments arranged by size:
3715 1570 794 584 426 321 272 141
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7843
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7808
DraI TTT'AAA
Cuts at: 5147 5147
Size: 7843
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4835 6850 809
Size: 507 3519 2015 1802
Fragments arranged by size:
3519 2015 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 2755 4113
Size: 54 1425 50 275 18 702 223 1358
Cuts at: 4113 4121 4273 4670 4986 5290 6179 7808 8
Size: 8 152 397 316 304 889 1629 43
Fragments arranged by size:
1629 1425 1358 889 702 397 316 304
275 223 152 54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 5290 1537
Size: 275 3478 4090
Fragments arranged by size:
4090 3478 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3399 3609 5773 1353
Size: 2046 210 2164 3423
Fragments arranged by size:
3423 2164 2046 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3197 3926 3998 4376 4478 6430
Size: 1064 138 1608 729 72 378 102 1952
Cuts at: 6430 6804 6950 387
Size: 374 146 1280
Fragments arranged by size:
1952 1608 1280 1064 729 378 374 146
138 102 72
EcoICRI GAG'CTC
Cuts at: 2776 3508 2776
Size: 732 7111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 2585 2747 2900 3044 3092 3192
Size: 393 840 113 162 153 144 48 100
Cuts at: 3192 3348 3384 3854 4052 4328 4346 4478 4787
Size: 156 36 470 198 276 18 132 309
Cuts at: 4787 5045 5906 6503 7290 7558 1239
Size: 258 861 597 787 268 1524
Fragments arranged by size:
1524 861 840 787 597 470 393 309
276 268 258 198 162 156 153 144
132 113 100 48 36 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2107 2213 2440 2576 3023 3299 3343 3701 4457
Size: 106 227 136 447 276 44 358 756
Cuts at: 4457 4849 2107
Size: 392 5101
Fragments arranged by size:
5101 756 447 392 358 276 227 136
106 44
EcoO109I rG'GnC_Cy
Cuts at: 2221 2472 2569 2581 2607 2712 2734 2850 3028
Size: 251 97 12 26 105 22 116 178
Cuts at: 3028 3058 3292 3348 3528 3571 4141 4450 4854
Size: 30 234 56 180 43 570 309 404
Cuts at: 4854 5125 2221
Size: 271 4939
Fragments arranged by size:
4939 570 404 309 271 251 234 180
178 116 105 97 56 43 30 26
22 12
EcoRI G'AATT_C
Cuts at: 5152 5530 5152
Size: 378 7465
EcoRV GAT'ATC
Cuts at: 5011 5011
Size: 7843
FspI TGC'GCA
Cuts at: 6349 6349
Size: 7843
HaeII r_GCGC'y
Cuts at: 1678 2335 3771 4224 4346 6990 1678
Size: 657 1436 453 122 2644 2531
Fragments arranged by size:
2644 2531 1436 657 453 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2494 2526 2558 2590 2639
Size: 32 135 32 922 32 32 32 49
Cuts at: 2639 2671 2689 2721 3296 3328 4667 4699 4747
Size: 32 18 32 575 32 1339 32 48
Cuts at: 4747 4779 6484 6516 6558 6590 1373
Size: 32 1705 32 42 32 2626
Fragments arranged by size:
2626 1705 1339 922 575 135 49 48
42 32 32 32 32 32 32 32
32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3817 5187 5433 669
Size: 988 281 1879 1370 246 3079
Fragments arranged by size:
3079 1879 1370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2354 748
Size: 1606 6237
HpaI GTT'AAC
Cuts at: 5433 5433
Size: 7843
KpnI G_GTAC'C
Cuts at: 5168 5168
Size: 7843
MfeI C'AATT_G
Cuts at: 3590 5442 3590
Size: 1852 5991
MluI A'CGCG_T
Cuts at: 7719 7719
Size: 7843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2569 4278 6957 7141 1305
Size: 1264 1709 2679 184 2007
Fragments arranged by size:
2679 2007 1709 1264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 4115 4988 10
Size: 54 1425 343 702 1581 873 2865
Fragments arranged by size:
2865 1581 1425 873 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 4701 4926 5801 6160 6319 518
Size: 648 861 2674 225 875 359 159 2042
Fragments arranged by size:
2674 2042 875 861 648 359 225 159
MspA1I CmG'CkG
Cuts at: 1606 2636 3494 3557 4558 5259 5936 7084 7329
Size: 1030 858 63 1001 701 677 1148 245
Cuts at: 7329 1606
Size: 2120
Fragments arranged by size:
2120 1148 1030 1001 858 701 677 245
63
NaeI GCC'GGC
Cuts at: 3045 3045
Size: 7843
NcoI C'CATG_G
Cuts at: 513 1065 2022 3124 513
Size: 552 957 1102 5232
Fragments arranged by size:
5232 1102 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7843
NgoMIV G'CCGG_C
Cuts at: 3043 3043
Size: 7843
NheI G'CTAG_C
Cuts at: 1052 4499 1052
Size: 3447 4396
NotI GC'GGCC_GC
Cuts at: 5290 5290
Size: 7843
NspI r_CATG'y
Cuts at: 1132 1229 5201 6746 1132
Size: 97 3972 1545 2229
Fragments arranged by size:
3972 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2109 3146 3160 4549 1348
Size: 761 1037 14 1389 4642
Fragments arranged by size:
4642 1389 1037 761 14
PciI A'CATG_T
Cuts at: 6742 6742
Size: 7843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2818 4111 1108
Size: 1710 1293 4840
Fragments arranged by size:
4840 1710 1293
PfoI T'CCnGG_A
Cuts at: 2562 4860 2562
Size: 2298 5545
PmeI GTTT'AAAC
Cuts at: 5147 5147
Size: 7843
PmlI CAC'GTG
Cuts at: 2376 2376
Size: 7843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4703 4735 5912 5944 7425 7457 4703
Size: 32 1177 32 1481 32 5089
Fragments arranged by size:
5089 1481 1177 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2607 2734 3028 4450 4854 2607
Size: 127 294 1422 404 5596
Fragments arranged by size:
5596 1422 404 294 127
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7843
PsiI TTA'TAA
Cuts at: 5413 5413
Size: 7843
PspOMI G'GGCC_C
Cuts at: 2711 3527 4141 2711
Size: 816 614 6413
Fragments arranged by size:
6413 816 614
PspXI vC'TCGA_Gb
Cuts at: 2213 3135 2213
Size: 922 6921
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4520 4552 4520
Size: 32 7811
PstI C_TGCA'G
Cuts at: 830 2331 3036 3303 3705 4779 5195 830
Size: 1501 705 267 402 1074 416 3478
Fragments arranged by size:
3478 1501 1074 705 416 402 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7843
SacI G_AGCT'C
Cuts at: 2778 3510 2778
Size: 732 7111
SalI G'TCGA_C
Cuts at: 1936 5185 1936
Size: 3249 4594
SbfI CC_TGCA'GG
Cuts at: 3303 3705 5195 3303
Size: 402 1490 5951
Fragments arranged by size:
5951 1490 402
ScaI AGT'ACT
Cuts at: 6091 7803 6091
Size: 1712 6131
SexAI A'CCwGG_T
Cuts at: 4713 4713
Size: 7843
SfcI C'TryA_G
Cuts at: 826 1045 2327 3032 3299 3701 4775 4891 5191
Size: 219 1282 705 267 402 1074 116 300
Cuts at: 5191 6326 7007 7198 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1282 1135 1074 705 681 402 300
267 219 191 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 3066 3066
Size: 7843
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7843
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7843
SmaI CCC'GGG
Cuts at: 1872 2710 2866 3487 4448 5170 1872
Size: 838 156 621 961 722 4545
Fragments arranged by size:
4545 961 838 722 621 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 2908 3135 3890 4849 5032
Size: 197 926 270 695 227 755 959 183
Cuts at: 5032 5120 5947 6848 7110 820
Size: 88 827 901 262 1553
Fragments arranged by size:
1553 959 926 901 827 755 695 270
262 227 197 183 88
SnaBI TAC'GTA
Cuts at: 493 5140 5634 493
Size: 4647 494 2702
Fragments arranged by size:
4647 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7843
SphI G_CATG'C
Cuts at: 1132 5201 1132
Size: 4069 3774
SspI AAT'ATT
Cuts at: 5 52 2042 5767 5
Size: 47 1990 3725 2081
Fragments arranged by size:
3725 2081 1990 47
StuI AGG'CCT
Cuts at: 1517 2243 2923 1517
Size: 726 680 6437
Fragments arranged by size:
6437 726 680
StyI C'CwwG_G
Cuts at: 513 1065 2022 3124 3192 5282 513
Size: 552 957 1102 68 2090 3074
Fragments arranged by size:
3074 2090 1102 957 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4146 4562 4845 5870 5887 6040 6225
Size: 1255 2566 416 283 1025 17 153 185
Cuts at: 6225 6670 325
Size: 445 1498
Fragments arranged by size:
2566 1498 1255 1025 445 416 283 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6089 7801 96
Size: 274 80 33 51 1443 4112 1712 138
Fragments arranged by size:
4112 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 3735 3767 4319 4331 4351 4363 7803
Size: 32 1544 32 552 12 20 12 3440
Cuts at: 7803 7835 2159
Size: 32 2167
Fragments arranged by size:
3440 2167 1544 552 32 32 32 20
12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7843
XbaI T'CTAG_A
Cuts at: 5179 5179
Size: 7843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2926 3457 3796 2926
Size: 531 339 6973
Fragments arranged by size:
6973 531 339
XhoI C'TCGA_G
Cuts at: 1943 2213 3135 1943
Size: 270 922 6651
Fragments arranged by size:
6651 922 270
XmaI C'CCGG_G
Cuts at: 1870 2708 2864 3485 4446 5168 1870
Size: 838 156 621 961 722 4545
Fragments arranged by size:
4545 961 838 722 621 156
XmnI GAAnn'nnTTC
Cuts at: 2084 5972 2084
Size: 3888 3955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3688 5651 276
Size: 53 83 186 936 2154 1963 2468
Fragments arranged by size:
2468 2154 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII
FalI FseI FspAI KasI NarI NruI NsiI PacI
PvuII RsrII SacII SanDI SapI SfoI SrfI SwaI