Restriction map for
pFN21ASDA0379
(Circular) (Six-base) MAPSORT of: pFN21ASDA0379.seq Check: 3353 from: 1 to: 7888
pFN21ASDA0379 7888 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5698 278
Size: 53 83 186 936 4162 2468
Fragments arranged by size:
4162 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3247 5231 1937
Size: 1310 1984 4594
Fragments arranged by size:
4594 1984 1310
Acc65I G'GTAC_C
Cuts at: 2401 5209 2401
Size: 2808 5080
AclI AA'CG_TT
Cuts at: 1563 6015 6388 1563
Size: 4452 373 3063
Fragments arranged by size:
4452 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 2634 3186 3569 5951 7335 7603 1632
Size: 780 222 552 383 2382 1384 268 1917
Fragments arranged by size:
2382 1917 1384 780 552 383 268 222
AflII C'TTAA_G
Cuts at: 820 1017 3163 820
Size: 197 2146 5545
Fragments arranged by size:
5545 2146 197
AflIII A'CryG_T
Cuts at: 1203 4331 6787 7764 1203
Size: 3128 2456 977 1327
Fragments arranged by size:
3128 2456 1327 977
AgeI A'CCGG_T
Cuts at: 6719 6719
Size: 7888
AhdI GACnn_n'nnGTC
Cuts at: 6617 6617
Size: 7888
AleI CACnn'nnGTG
Cuts at: 1166 4218 5144 1166
Size: 3052 926 3910
Fragments arranged by size:
3910 3052 926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2874 3372 3461 4836 1184
Size: 1690 498 89 1375 4236
Fragments arranged by size:
4236 1690 1375 498 89
AlwNI CAG_nnn'CTG
Cuts at: 3382 4546 5631 7203 3382
Size: 1164 1085 1572 4067
Fragments arranged by size:
4067 1572 1164 1085
ApaLI G'TGCA_C
Cuts at: 5945 7101 5945
Size: 1156 6732
ApoI r'AATT_y
Cuts at: 1493 3271 3329 5197 5423 5575 7684 1493
Size: 1778 58 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1778 1697 226 152 58
AseI AT'TA_AT
Cuts at: 160 1032 2520 3831 3929 6442 160
Size: 872 1488 1311 98 2513 1606
Fragments arranged by size:
2513 1606 1488 1311 872 98
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 5213 1870
Size: 73 385 2885 4545
Fragments arranged by size:
4545 2885 385 73
BamHI G'GATC_C
Cuts at: 3519 5218 5585 3519
Size: 1699 367 5822
Fragments arranged by size:
5822 1699 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2401 2510 4457 5209 6664
Size: 325 217 627 614 109 1947 752 1455
Cuts at: 6664 618
Size: 1842
Fragments arranged by size:
1947 1842 1455 752 627 614 325 217
109
BanII G_rGCy'C
Cuts at: 1391 4234 5157 5207 1391
Size: 2843 923 50 4072
Fragments arranged by size:
4072 2843 923 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3575 928
Size: 971 1676 5241
Fragments arranged by size:
5241 1676 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4412 4446 6079 6113 1903
Size: 34 2475 34 1633 34 3678
Fragments arranged by size:
3678 2475 1633 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 5780 6990 3530
Size: 2250 1210 4428
Fragments arranged by size:
4428 2250 1210
BclI T'GATC_A
Cuts at: 1548 2676 3084 1548
Size: 1128 408 6352
Fragments arranged by size:
6352 1128 408
BfrBI ATG'CAT
Cuts at: 4133 4133
Size: 7888
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6499 136
Size: 107 122 71 631 605 4827 1525
Fragments arranged by size:
4827 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2701 3338 3745 3784 7883 2701
Size: 637 407 39 4099 2706
Fragments arranged by size:
4099 2706 637 407 39
BlpI GC'TnA_GC
Cuts at: 4550 5305 4550
Size: 755 7133
Bme1580I G_kGCm'C
Cuts at: 1790 4017 5949 7105 1790
Size: 2227 1932 1156 2573
Fragments arranged by size:
2573 2227 1932 1156
BmgBI CAC'GTC
Cuts at: 1318 5146 1318
Size: 3828 4060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4315 5619 6577
Size: 416 53 83 399 24 2898 1304 958
Cuts at: 6577 442
Size: 1753
Fragments arranged by size:
2898 1753 1304 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 3285 6548 1239
Size: 993 1053 3263 2579
Fragments arranged by size:
3263 2579 1053 993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4548 6013 6878 7176 4548
Size: 1465 865 298 5260
Fragments arranged by size:
5260 1465 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5168 6551 882
Size: 617 3669 1383 2219
Fragments arranged by size:
3669 2219 1383 617
BsaAI yAC'GTr
Cuts at: 493 1204 5185 5679 493
Size: 711 3981 494 2702
Fragments arranged by size:
3981 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3510 4263 7882 3510
Size: 753 3619 3516
Fragments arranged by size:
3619 3516 753
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3986 4184 5695 6077
Size: 53 83 186 936 2453 198 1511 382
Cuts at: 6077 6712 275
Size: 635 1451
Fragments arranged by size:
2453 1511 1451 936 635 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 6321 6719 6993 7140 7551 6321
Size: 398 274 147 411 6658
Fragments arranged by size:
6658 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2205 2235 2601 2631 3396
Size: 30 165 30 802 30 366 30 765
Cuts at: 3396 3426 4041 4071 4860 4890 1178
Size: 30 615 30 789 30 4176
Fragments arranged by size:
4176 802 789 765 615 366 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3597 4488 1186
Size: 2411 891 4586
Fragments arranged by size:
4586 2411 891
BseYI C'CCAG_C
Cuts at: 4906 7091 7587 4906
Size: 2185 496 5207
Fragments arranged by size:
5207 2185 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3327 3332 3421 3431 4683 1163
Size: 573 1591 5 89 10 1252 4368
Fragments arranged by size:
4368 1591 1252 573 89 10 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5338 6099 7127 1540
Size: 275 186 3337 761 1028 2301
Fragments arranged by size:
3337 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2101 4924 5157 5949 6034 7105 2101
Size: 2823 233 792 85 1071 2884
Fragments arranged by size:
2884 2823 1071 792 233 85
BsmI GAATG_Cn'
Cuts at: 2308 3920 3958 5399 5492 2308
Size: 1612 38 1441 93 4704
Fragments arranged by size:
4704 1612 1441 93 38
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 4017 4234 4924 5157 5207 5949
Size: 399 311 1916 217 690 233 50 742
Cuts at: 5949 6034 7105 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1916 1071 742 690 399 311 233
217 85 50
BspEI T'CCGG_A
Cuts at: 7551 7551
Size: 7888
BspHI T'CATG_A
Cuts at: 4726 5073 5777 7507 4726
Size: 347 704 1730 5107
Fragments arranged by size:
5107 1730 704 347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5243 844
Size: 4399 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5775 1394
Size: 429 3952 3507
Fragments arranged by size:
3952 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2153 2911 4034 4394 6383 6557 65
Size: 1183 905 758 1123 360 1989 174 1396
Fragments arranged by size:
1989 1396 1183 1123 905 758 360 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6532 6719 1700
Size: 110 4722 187 2869
Fragments arranged by size:
4722 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7888
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7888
BssSI C'ACGA_G
Cuts at: 4325 5948 6960 4325
Size: 1623 1012 5253
Fragments arranged by size:
5253 1623 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7888
BstBI TT'CG_AA
Cuts at: 2007 6692 2007
Size: 4685 3203
BstEII G'GTnAC_C
Cuts at: 1184 3569 3704 1184
Size: 2385 135 5368
Fragments arranged by size:
5368 2385 135
BstYI r'GATC_y
Cuts at: 1972 2080 2701 3338 3519 3745 3784 5218 5585
Size: 108 621 637 181 226 39 1434 367
Cuts at: 5585 5970 5987 7439 7525 7537 7883 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1434 637 621 385 367 346
226 181 108 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3631 3631
Size: 7888
BtgI C'CryG_G
Cuts at: 513 1065 2022 2968 513
Size: 552 957 946 5433
Fragments arranged by size:
5433 957 946 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7149
Fragments arranged by size:
7149 683 56
BtsI GCAGTG_nn'
Cuts at: 3114 3344 3963 4660 5404 6198 6218 3114
Size: 230 619 697 744 794 20 4784
Fragments arranged by size:
4784 794 744 697 619 230 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7853
DraI TTT'AAA
Cuts at: 5192 5192
Size: 7888
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6895 809
Size: 507 5579 1802
Fragments arranged by size:
5579 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 4962 5335
Size: 54 1425 50 275 18 1240 1892 373
Cuts at: 5335 6224 7853 8
Size: 889 1629 43
Fragments arranged by size:
1892 1629 1425 1240 889 373 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5335 1537
Size: 275 3523 4090
Fragments arranged by size:
4090 3523 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2412 2655 2754 2921 3866 5818 1353
Size: 1059 243 99 167 945 1952 3423
Fragments arranged by size:
3423 1952 1059 945 243 167 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6475 6849 6995 387
Size: 1064 138 4886 374 146 1280
Fragments arranged by size:
4886 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5155 5155
Size: 7888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2412 2634 3186 3285 3569 5951
Size: 393 600 180 222 552 99 284 2382
Cuts at: 5951 6548 7335 7603 1239
Size: 597 787 268 1524
Fragments arranged by size:
2382 1524 787 600 597 552 393 284
268 222 180 99
EcoRI G'AATT_C
Cuts at: 5197 5575 5197
Size: 378 7510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5010 5010
Size: 7888
FspI TGC'GCA
Cuts at: 6394 6394
Size: 7888
HaeII r_GCGC'y
Cuts at: 1678 7035 1678
Size: 5357 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2621 2653 3000
Size: 32 135 32 464 32 553 32 347
Cuts at: 3000 3032 3076 3108 3831 3863 6529 6561 6603
Size: 32 44 32 723 32 2666 32 42
Cuts at: 6603 6635 1373
Size: 32 2626
Fragments arranged by size:
2666 2626 723 553 464 347 135 44
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 2247 5232 5478 669
Size: 988 281 187 122 2985 246 3079
Fragments arranged by size:
3079 2985 988 281 246 187 122
HindIII A'AGCT_T
Cuts at: 748 2394 5061 748
Size: 1646 2667 3575
Fragments arranged by size:
3575 2667 1646
HpaI GTT'AAC
Cuts at: 2125 5478 2125
Size: 3353 4535
KpnI G_GTAC'C
Cuts at: 2405 5213 2405
Size: 2808 5080
MfeI C'AATT_G
Cuts at: 5487 5487
Size: 7888
MluI A'CGCG_T
Cuts at: 7764 7764
Size: 7888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7002 7186 1305
Size: 5697 184 2007
Fragments arranged by size:
5697 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3072 4964 10
Size: 54 1425 343 1240 1892 2934
Fragments arranged by size:
2934 1892 1425 1240 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4218 4970 5144 5846 6205 6364 518
Size: 648 3052 752 174 702 359 159 2042
Fragments arranged by size:
3052 2042 752 702 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 3257 3263 3560 3589 4543 5304 5981 7129
Size: 1651 6 297 29 954 761 677 1148
Cuts at: 7129 7374 1606
Size: 245 2120
Fragments arranged by size:
2120 1651 1148 954 761 677 297 245
29 6
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6379
Fragments arranged by size:
6379 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7888
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7888
NotI GC'GGCC_GC
Cuts at: 5335 5335
Size: 7888
NsiI A_TGCA'T
Cuts at: 4135 4135
Size: 7888
NspI r_CATG'y
Cuts at: 1132 1229 2625 3254 3646 4335 4817 5246 6791
Size: 97 1396 629 392 689 482 429 1545
Cuts at: 6791 1132
Size: 2229
Fragments arranged by size:
2229 1545 1396 689 629 482 429 392
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7888
PciI A'CATG_T
Cuts at: 4331 6787 4331
Size: 2456 5432
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7888
PmeI GTTT'AAAC
Cuts at: 5192 5192
Size: 7888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5957 5989 7470 7502 5957
Size: 32 1481 32 6343
Fragments arranged by size:
6343 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7888
PsiI TTA'TAA
Cuts at: 5458 5458
Size: 7888
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4659 4691 4659
Size: 32 7856
PstI C_TGCA'G
Cuts at: 830 2317 3188 3262 4615 5240 830
Size: 1487 871 74 1353 625 3478
Fragments arranged by size:
3478 1487 1353 871 625 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7888
PvuII CAG'CTG
Cuts at: 3257 3263 3560 3589 4543 3257
Size: 6 297 29 954 6602
Fragments arranged by size:
6602 954 297 29 6
SacI G_AGCT'C
Cuts at: 5157 5157
Size: 7888
SalI G'TCGA_C
Cuts at: 1936 5230 1936
Size: 3294 4594
SbfI CC_TGCA'GG
Cuts at: 5240 5240
Size: 7888
ScaI AGT'ACT
Cuts at: 5132 6136 7848 5132
Size: 1004 1712 5172
Fragments arranged by size:
5172 1712 1004
SfcI C'TryA_G
Cuts at: 826 1045 2313 3184 3258 4584 4611 5236 6371
Size: 219 1268 871 74 1326 27 625 1135
Cuts at: 6371 7052 7243 826
Size: 681 191 1471
Fragments arranged by size:
1471 1326 1268 1135 871 681 625 219
191 74 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7888
SmaI CCC'GGG
Cuts at: 1872 5215 1872
Size: 3343 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2328 3163 4563 5992 6893 7155
Size: 197 926 385 835 1400 1429 901 262
Cuts at: 7155 820
Size: 1553
Fragments arranged by size:
1553 1429 1400 926 901 835 385 262
197
SnaBI TAC'GTA
Cuts at: 493 5185 5679 493
Size: 4692 494 2702
Fragments arranged by size:
4692 2702 494
SpeI A'CTAG_T
Cuts at: 152 4733 152
Size: 4581 3307
SphI G_CATG'C
Cuts at: 1132 5246 1132
Size: 4114 3774
SspI AAT'ATT
Cuts at: 5 52 2106 2518 5812 5
Size: 47 2054 412 3294 2081
Fragments arranged by size:
3294 2081 2054 412 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7888
StyI C'CwwG_G
Cuts at: 513 1065 2022 2173 5327 513
Size: 552 957 151 3154 3074
Fragments arranged by size:
3154 3074 957 552 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5915 5932 6085 6270 6715 325
Size: 1255 4335 17 153 185 445 1498
Fragments arranged by size:
4335 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3823 5130 6134
Size: 274 80 33 51 1443 1846 1307 1004
Cuts at: 6134 7846 96
Size: 1712 138
Fragments arranged by size:
1846 1712 1443 1307 1004 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2601 2633 3396 3428 4860 4892 7848 7880 2601
Size: 32 763 32 1432 32 2956 32 2609
Fragments arranged by size:
2956 2609 1432 763 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7888
XbaI T'CTAG_A
Cuts at: 2504 2698 3610 5224 2504
Size: 194 912 1614 5168
Fragments arranged by size:
5168 1614 912 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4933 4933
Size: 7888
XhoI C'TCGA_G
Cuts at: 1943 2328 1943
Size: 385 7503
XmaI C'CCGG_G
Cuts at: 1870 5213 1870
Size: 3343 4545
XmnI GAAnn'nnTTC
Cuts at: 2289 6017 2289
Size: 3728 4160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5696 276
Size: 53 83 186 936 4162 2468
Fragments arranged by size:
4162 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI