Restriction map for
pFN21ASDA0378
(Circular) (Six-base) MAPSORT of: pFN21ASDA0378.seq Check: 4508 from: 1 to: 7600
pFN21ASDA0378 7600 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5410 278
Size: 53 83 186 936 3874 2468
Fragments arranged by size:
3874 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4943 1937
Size: 3006 4594
Acc65I G'GTAC_C
Cuts at: 4921 4921
Size: 7600
AclI AA'CG_TT
Cuts at: 1563 5727 6100 1563
Size: 4164 373 3063
Fragments arranged by size:
4164 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 3104 3359 3470 3638 5663 7047 7315
Size: 824 648 255 111 168 2025 1384 268
Cuts at: 7315 1632
Size: 1917
Fragments arranged by size:
2025 1917 1384 824 648 268 255 168
111
AfeI AGC'GCT
Cuts at: 3818 3818
Size: 7600
AflII C'TTAA_G
Cuts at: 820 1017 2591 820
Size: 197 1574 5829
Fragments arranged by size:
5829 1574 197
AflIII A'CryG_T
Cuts at: 1203 6499 7476 1203
Size: 5296 977 1327
Fragments arranged by size:
5296 1327 977
AgeI A'CCGG_T
Cuts at: 4203 6431 4203
Size: 2228 5372
AhdI GACnn_n'nnGTC
Cuts at: 6329 6329
Size: 7600
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7600
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7600
AlwNI CAG_nnn'CTG
Cuts at: 3026 3764 4468 4510 5343 6915 3026
Size: 738 704 42 833 1572 3711
Fragments arranged by size:
3711 1572 833 738 704 42
ApaLI G'TGCA_C
Cuts at: 5657 6813 5657
Size: 1156 6444
ApoI r'AATT_y
Cuts at: 1493 3412 3600 4909 5135 5287 7396 1493
Size: 1919 188 1309 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 1309 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 6154 160
Size: 872 5122 1606
Fragments arranged by size:
5122 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7600
AvaI C'yCGr_G
Cuts at: 1870 1943 2922 4925 1870
Size: 73 979 2003 4545
Fragments arranged by size:
4545 2003 979 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2192 2225 2192
Size: 33 7567
BamHI G'GATC_C
Cuts at: 4930 5297 4930
Size: 367 7233
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4921 6376 618
Size: 325 217 627 3134 1455 1842
Fragments arranged by size:
3134 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4919 1391
Size: 3528 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2873 3326 4398 4460 928
Size: 971 974 453 1072 62 4068
Fragments arranged by size:
4068 1072 974 971 453 62
BbvCI CC'TCA_GC
Cuts at: 3401 3401
Size: 7600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5791 5825 1903
Size: 34 3854 34 3678
Fragments arranged by size:
3854 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5492 6702 5492
Size: 1210 6390
BclI T'GATC_A
Cuts at: 1548 2218 3331 1548
Size: 670 1113 5817
Fragments arranged by size:
5817 1113 670
BfrBI ATG'CAT
Cuts at: 3971 3971
Size: 7600
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6211 136
Size: 107 122 71 631 605 4539 1525
Fragments arranged by size:
4539 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2467 7595 2467
Size: 5128 2472
BlpI GC'TnA_GC
Cuts at: 5017 5017
Size: 7600
Bme1580I G_kGCm'C
Cuts at: 1790 2258 5661 6817 1790
Size: 468 3403 1156 2573
Fragments arranged by size:
3403 2573 1156 468
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5331 6289 442
Size: 416 53 83 399 24 3914 958 1753
Fragments arranged by size:
3914 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4299 4299
Size: 7600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2599 2732 3008 3134 4090 4232 4292 4469
Size: 1360 133 276 126 956 142 60 177
Cuts at: 4469 4586 4598 6260 1239
Size: 117 12 1662 2579
Fragments arranged by size:
2579 1662 1360 956 276 177 142 133
126 117 60 12
Bpu10I CC'TnA_GC
Cuts at: 2586 2844 3401 2586
Size: 258 557 6785
Fragments arranged by size:
6785 557 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2629 2969 3233 4205 4310 4676 5725 6590
Size: 2 340 264 972 105 366 1049 865
Cuts at: 6590 6888 2627
Size: 298 3339
Fragments arranged by size:
3339 1049 972 865 366 340 298 264
105 2
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2097 2724 6263 882
Size: 617 598 627 3539 2219
Fragments arranged by size:
3539 2219 627 617 598
BsaAI yAC'GTr
Cuts at: 493 1204 4897 5391 493
Size: 711 3693 494 2702
Fragments arranged by size:
3693 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4779 7594 4779
Size: 2815 4785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4157 5407 5789 6424
Size: 53 83 186 936 2624 1250 382 635
Cuts at: 6424 275
Size: 1451
Fragments arranged by size:
2624 1451 1250 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2514 4203 4542 4861 6033 6431 6705 6852 7263
Size: 1689 339 319 1172 398 274 147 411
Cuts at: 7263 2514
Size: 2851
Fragments arranged by size:
2851 1689 1172 411 398 339 319 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2289 2319 1178
Size: 30 165 30 886 30 6459
Fragments arranged by size:
6459 886 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2501 2735 2784 2816 4567 4583 4892
Size: 899 416 234 49 32 1751 16 309
Cuts at: 4892 1186
Size: 3894
Fragments arranged by size:
3894 1751 899 416 309 234 49 32
16
BseYI C'CCAG_C
Cuts at: 2478 6803 7299 2478
Size: 4325 496 2779
Fragments arranged by size:
4325 2779 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3750 1163
Size: 573 2014 5013
Fragments arranged by size:
5013 2014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5050 5811 6839 1540
Size: 275 186 3049 761 1028 2301
Fragments arranged by size:
3049 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2781 3444 5661 5746 6817 2781
Size: 663 2217 85 1071 3564
Fragments arranged by size:
3564 2217 1071 663 85
BsmI GAATG_Cn'
Cuts at: 4447 5111 5204 4447
Size: 664 93 6843
Fragments arranged by size:
6843 664 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2781 3444 4919 5661 5746 6817
Size: 399 468 523 663 1475 742 85 1071
Cuts at: 6817 1391
Size: 2174
Fragments arranged by size:
2174 1475 1071 742 663 523 468 399
85
BspEI T'CCGG_A
Cuts at: 2514 4542 4861 7263 2514
Size: 2028 319 2402 2851
Fragments arranged by size:
2851 2402 2028 319
BspHI T'CATG_A
Cuts at: 2221 5489 7219 2221
Size: 3268 1730 2602
Fragments arranged by size:
3268 2602 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 4955 844
Size: 1590 2521 3489
Fragments arranged by size:
3489 2521 1590
BsrBI CCG'CTC
Cuts at: 1394 1823 2774 5487 1394
Size: 429 951 2713 3507
Fragments arranged by size:
3507 2713 951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4629 6095 6269 65
Size: 1183 3381 1466 174 1396
Fragments arranged by size:
3381 1466 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3593 4203 6244 6431 1700
Size: 110 1783 610 2041 187 2869
Fragments arranged by size:
2869 2041 1783 610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7600
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7600
BssSI C'ACGA_G
Cuts at: 5660 6672 5660
Size: 1012 6588
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2189 4601 4631 794
Size: 1395 2412 30 3763
Fragments arranged by size:
3763 2412 1395 30
BstBI TT'CG_AA
Cuts at: 2007 6404 2007
Size: 4397 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7600
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 2986
Size: 7600
BstYI r'GATC_y
Cuts at: 1972 2072 2467 2549 3208 3222 4215 4572 4930
Size: 100 395 82 659 14 993 357 358
Cuts at: 4930 5297 5682 5699 7151 7237 7249 7595 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 993 659 395 385 367 358
357 346 100 86 82 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2313 513
Size: 552 1248 5800
Fragments arranged by size:
5800 1248 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6861
Fragments arranged by size:
6861 683 56
BtsI GCAGTG_nn'
Cuts at: 5116 5910 5930 5116
Size: 794 20 6786
Fragments arranged by size:
6786 794 20
ClaI AT'CG_AT
Cuts at: 1554 4819 1554
Size: 3265 4335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7565
DraI TTT'AAA
Cuts at: 4904 4904
Size: 7600
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6607 809
Size: 507 5291 1802
Fragments arranged by size:
5291 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3591 4317 4512
Size: 54 1425 50 275 18 1761 726 195
Cuts at: 4512 4533 5047 5936 7565 8
Size: 21 514 889 1629 43
Fragments arranged by size:
1761 1629 1425 889 726 514 275 195
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 5047 1537
Size: 275 3235 4090
Fragments arranged by size:
4090 3235 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2613 3267 3570 3855 5530 1353
Size: 1260 654 303 285 1675 3423
Fragments arranged by size:
3423 1675 1260 654 303 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3028 6187 6561 6707 387
Size: 1064 138 1439 3159 374 146 1280
Fragments arranged by size:
3159 1439 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2599 2732 3008 3104 3134 3359
Size: 393 824 143 133 276 96 30 225
Cuts at: 3359 3470 3638 4090 4232 4292 4469 4586 4598
Size: 111 168 452 142 60 177 117 12
Cuts at: 4598 5663 6260 7047 7315 1239
Size: 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 824 787 597 452 393 276
268 225 177 168 143 142 133 117
111 96 60 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4223 4223
Size: 7600
EcoO109I rG'GnC_Cy
Cuts at: 2425 2701 3565 4854 2425
Size: 276 864 1289 5171
Fragments arranged by size:
5171 1289 864 276
EcoRI G'AATT_C
Cuts at: 4909 5287 4909
Size: 378 7222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3470 3701 4616 3470
Size: 231 915 6454
Fragments arranged by size:
6454 915 231
FspI TGC'GCA
Cuts at: 4382 6106 4382
Size: 1724 5876
HaeII r_GCGC'y
Cuts at: 1678 3820 6747 1678
Size: 2142 2927 2531
Fragments arranged by size:
2927 2531 2142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2289 2321 3014 3046 3078
Size: 32 135 32 717 32 693 32 32
Cuts at: 3078 3130 3162 3854 3886 3926 3958 6241 6273
Size: 52 32 692 32 40 32 2283 32
Cuts at: 6273 6315 6347 1373
Size: 42 32 2626
Fragments arranged by size:
2626 2283 717 693 692 135 52 42
40 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 4944 5190 669
Size: 988 281 753 2253 246 3079
Fragments arranged by size:
3079 2253 988 753 281 246
HindIII A'AGCT_T
Cuts at: 748 3389 748
Size: 2641 4959
HpaI GTT'AAC
Cuts at: 5190 5190
Size: 7600
KpnI G_GTAC'C
Cuts at: 4925 4925
Size: 7600
MfeI C'AATT_G
Cuts at: 5199 5199
Size: 7600
MluI A'CGCG_T
Cuts at: 7476 7476
Size: 7600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3345 4323 6714 6898 1305
Size: 2040 978 2391 184 2007
Fragments arranged by size:
2391 2040 2007 978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4319 4535 10
Size: 54 1425 343 2487 216 3075
Fragments arranged by size:
3075 2487 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2358 3164 3437 5558 5917 6076
Size: 648 1146 46 806 273 2121 359 159
Cuts at: 6076 518
Size: 2042
Fragments arranged by size:
2121 2042 1146 806 648 359 273 159
46
MspA1I CmG'CkG
Cuts at: 1606 2482 3337 4564 5016 5693 6841 7086 1606
Size: 876 855 1227 452 677 1148 245 2120
Fragments arranged by size:
2120 1227 1148 876 855 677 452 245
NcoI C'CATG_G
Cuts at: 513 1065 2313 513
Size: 552 1248 5800
Fragments arranged by size:
5800 1248 552
NdeI CA'TA_TG
Cuts at: 387 4293 4886 387
Size: 3906 593 3101
Fragments arranged by size:
3906 3101 593
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7600
NotI GC'GGCC_GC
Cuts at: 5047 5047
Size: 7600
NsiI A_TGCA'T
Cuts at: 3973 3973
Size: 7600
NspI r_CATG'y
Cuts at: 1132 1229 3971 4958 6503 1132
Size: 97 2742 987 1545 2229
Fragments arranged by size:
2742 2229 1545 987 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7600
PciI A'CATG_T
Cuts at: 6499 6499
Size: 7600
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7600
PfoI T'CCnGG_A
Cuts at: 4106 4575 4106
Size: 469 7131
PmeI GTTT'AAAC
Cuts at: 4904 4904
Size: 7600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5669 5701 7182 7214 5669
Size: 32 1481 32 6055
Fragments arranged by size:
6055 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 3565 2425
Size: 1140 6460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7600
PsiI TTA'TAA
Cuts at: 5170 5170
Size: 7600
PstI C_TGCA'G
Cuts at: 830 2496 4952 830
Size: 1666 2456 3478
Fragments arranged by size:
3478 2456 1666
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7600
PvuII CAG'CTG
Cuts at: 2482 3337 4564 2482
Size: 855 1227 5518
Fragments arranged by size:
5518 1227 855
SalI G'TCGA_C
Cuts at: 1936 4942 1936
Size: 3006 4594
SbfI CC_TGCA'GG
Cuts at: 4952 4952
Size: 7600
ScaI AGT'ACT
Cuts at: 5848 7560 5848
Size: 1712 5888
SexAI A'CCwGG_T
Cuts at: 2421 2421
Size: 7600
SfcI C'TryA_G
Cuts at: 826 1045 2492 2684 3560 4948 6083 6764 6955
Size: 219 1447 192 876 1388 1135 681 191
Cuts at: 6955 826
Size: 1471
Fragments arranged by size:
1471 1447 1388 1135 876 681 219 192
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7600
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7600
SmaI CCC'GGG
Cuts at: 1872 4927 1872
Size: 3055 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 2608 2642 2948 3212 4220
Size: 197 926 648 17 34 306 264 1008
Cuts at: 4220 4325 4691 5704 6605 6867 820
Size: 105 366 1013 901 262 1553
Fragments arranged by size:
1553 1013 1008 926 901 648 366 306
264 262 197 105 34 17
SnaBI TAC'GTA
Cuts at: 493 4897 5391 493
Size: 4404 494 2702
Fragments arranged by size:
4404 2702 494
SpeI A'CTAG_T
Cuts at: 152 4711 152
Size: 4559 3041
SphI G_CATG'C
Cuts at: 1132 4958 1132
Size: 3826 3774
SspI AAT'ATT
Cuts at: 5 52 2332 4093 5524 5
Size: 47 2280 1761 1431 2081
Fragments arranged by size:
2280 2081 1761 1431 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7600
StyI C'CwwG_G
Cuts at: 513 1065 2087 2313 5039 513
Size: 552 1022 226 2726 3074
Fragments arranged by size:
3074 2726 1022 552 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2813 4722 5627 5644 5797 5982 6427
Size: 1255 1233 1909 905 17 153 185 445
Cuts at: 6427 325
Size: 1498
Fragments arranged by size:
1909 1498 1255 1233 905 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4714 5846 7558
Size: 274 80 33 51 1443 2737 1132 1712
Cuts at: 7558 96
Size: 138
Fragments arranged by size:
2737 1712 1443 1132 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7560 7592 7560
Size: 32 7568
Tth111I GACn'n_nGTC
Cuts at: 1316 2152 1316
Size: 836 6764
XbaI T'CTAG_A
Cuts at: 4021 4936 4021
Size: 915 6685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2756 4465 4823 2756
Size: 1709 358 5533
Fragments arranged by size:
5533 1709 358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7600
XmaI C'CCGG_G
Cuts at: 1870 4925 1870
Size: 3055 4545
XmnI GAAnn'nnTTC
Cuts at: 5729 5729
Size: 7600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5408 276
Size: 53 83 186 936 3874 2468
Fragments arranged by size:
3874 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI