Restriction map for
pFN21ASDA0364
(Circular) (Six-base) MAPSORT of: pFN21ASDA0364.seq Check: 255 from: 1 to: 8710
pFN21ASDA0364 8710 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6520 278
Size: 53 83 186 936 4984 2468
Fragments arranged by size:
4984 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6053 1937
Size: 4116 4594
Acc65I G'GTAC_C
Cuts at: 6031 6031
Size: 8710
AclI AA'CG_TT
Cuts at: 1563 6837 7210 1563
Size: 5274 373 3063
Fragments arranged by size:
5274 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2228 2666 3296 3521 3890 5234 5522
Size: 449 147 438 630 225 369 1344 288
Cuts at: 5522 6773 8157 8425 1632
Size: 1251 1384 268 1917
Fragments arranged by size:
1917 1384 1344 1251 630 449 438 369
288 268 225 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8513
AflIII A'CryG_T
Cuts at: 1203 7609 8586 1203
Size: 6406 977 1327
Fragments arranged by size:
6406 1327 977
AgeI A'CCGG_T
Cuts at: 2307 7541 2307
Size: 5234 3476
AhdI GACnn_n'nnGTC
Cuts at: 7439 7439
Size: 8710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 3600 4191 1184
Size: 1032 1384 591 5703
Fragments arranged by size:
5703 1384 1032 591
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4080 4112 4716 4748 4080
Size: 32 604 32 8042
Fragments arranged by size:
8042 604 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2507 4464 4643 5109 5750 6453 8025 2507
Size: 1957 179 466 641 703 1572 3192
Fragments arranged by size:
3192 1957 1572 703 641 466 179
ApaI G_GGCC'C
Cuts at: 2703 2703
Size: 8710
ApaLI G'TGCA_C
Cuts at: 5764 6767 7923 5764
Size: 1003 1156 6551
Fragments arranged by size:
6551 1156 1003
ApoI r'AATT_y
Cuts at: 1493 2833 3380 4604 5567 6019 6245 6397 8506
Size: 1340 547 1224 963 452 226 152 2109
Cuts at: 8506 1493
Size: 1697
Fragments arranged by size:
2109 1697 1340 1224 963 547 452 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7264 160
Size: 872 6232 1606
Fragments arranged by size:
6232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8710
AvaI C'yCGr_G
Cuts at: 1870 1943 4374 6035 1870
Size: 73 2431 1661 4545
Fragments arranged by size:
4545 2431 1661 73
BamHI G'GATC_C
Cuts at: 2232 5039 6040 6407 2232
Size: 2807 1001 367 4535
Fragments arranged by size:
4535 2807 1001 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2276 2368 2710 2736
Size: 325 217 627 379 110 92 342 26
Cuts at: 2736 3938 4370 4965 5939 6031 7486 618
Size: 1202 432 595 974 92 1455 1842
Fragments arranged by size:
1842 1455 1202 974 627 595 432 379
342 325 217 110 92 92 26
BanII G_rGCy'C
Cuts at: 1391 2634 2703 3498 4008 4287 4575 4593 4729
Size: 1243 69 795 510 279 288 18 136
Cuts at: 4729 4926 5305 6029 1391
Size: 197 379 724 4072
Fragments arranged by size:
4072 1243 795 724 510 379 288 279
197 136 69 18
BbeI G_GCGC'C
Cuts at: 3942 3942
Size: 8710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2069 2790 2819 3078 3197 5382 928
Size: 971 170 721 29 259 119 2185 4256
Fragments arranged by size:
4256 2185 971 721 259 170 119 29
BbvCI CC'TCA_GC
Cuts at: 5601 5601
Size: 8710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4687 4721 6901 6935 1903
Size: 34 2750 34 2180 34 3678
Fragments arranged by size:
3678 2750 2180 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4985 6602 7812 4985
Size: 1617 1210 5883
Fragments arranged by size:
5883 1617 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3843 7321 136
Size: 107 122 71 631 605 2171 3478 1525
Fragments arranged by size:
3478 2171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5454 8705 5454
Size: 3251 5459
BlpI GC'TnA_GC
Cuts at: 4920 6127 4920
Size: 1207 7503
Bme1580I G_kGCm'C
Cuts at: 1790 2281 2703 4375 5768 6771 7927 1790
Size: 491 422 1672 1393 1003 1156 2573
Fragments arranged by size:
2573 1672 1393 1156 1003 491 422
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 2538 2976
Size: 416 53 83 399 24 923 198 438
Cuts at: 2976 4002 4281 4920 6441 7399 442
Size: 1026 279 639 1521 958 1753
Fragments arranged by size:
1753 1521 1026 958 923 639 438 416
399 279 198 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5834 1056
Size: 4778 3932
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2930
Size: 8710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2736 3359 4319 5471 5669 5747 7370 1239
Size: 1497 623 960 1152 198 78 1623 2579
Fragments arranged by size:
2579 1623 1497 1152 960 623 198 78
Bpu10I CC'TnA_GC
Cuts at: 3765 3771 4326 4902 5304 5601 5757 3765
Size: 6 555 576 402 297 156 6718
Fragments arranged by size:
6718 576 555 402 297 156 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3651 4040 6835 7700 7998 3651
Size: 389 2795 865 298 4363
Fragments arranged by size:
4363 2795 865 389 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 3779 5886 7373 882
Size: 617 906 1374 2107 1487 2219
Fragments arranged by size:
2219 2107 1487 1374 906 617
BsaAI yAC'GTr
Cuts at: 493 1204 6007 6501 493
Size: 711 4803 494 2702
Fragments arranged by size:
4803 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8704 8704
Size: 8710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3939 6517 6899 7534
Size: 53 83 186 936 2406 2578 382 635
Cuts at: 7534 275
Size: 1451
Fragments arranged by size:
2578 2406 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2107 2307 4098 7143 7541 7815 7962 8373 2107
Size: 200 1791 3045 398 274 147 411 2444
Fragments arranged by size:
3045 2444 1791 411 398 274 200 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3848 3878 4080 4110 4608
Size: 30 165 30 2445 30 202 30 498
Cuts at: 4608 4638 4716 4746 5429 5459 5613 5643 1178
Size: 30 78 30 683 30 154 30 4245
Fragments arranged by size:
4245 2445 683 498 202 165 154 78
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4001 1186
Size: 2815 5895
BseYI C'CCAG_C
Cuts at: 2633 3655 4574 5195 5208 5501 7913 8409 2633
Size: 1022 919 621 13 293 2412 496 2934
Fragments arranged by size:
2934 2412 1022 919 621 496 293 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3850 4506 4985 1163
Size: 573 2114 656 479 4888
Fragments arranged by size:
4888 2114 656 573 479
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6160 6921 7949 1540
Size: 275 186 4159 761 1028 2301
Fragments arranged by size:
4159 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4593 5768 6771 6856 7927 4593
Size: 1175 1003 85 1071 5376
Fragments arranged by size:
5376 1175 1071 1003 85
BsmI GAATG_Cn'
Cuts at: 2088 5060 6221 6314 2088
Size: 2972 1161 93 4484
Fragments arranged by size:
4484 2972 1161 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 2634 2703 3498 4008 4287 4375
Size: 399 491 353 69 795 510 279 88
Cuts at: 4375 4575 4593 4729 4926 5305 5768 6029 6771
Size: 200 18 136 197 379 463 261 742
Cuts at: 6771 6856 7927 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 795 742 510 491 463 399
379 353 279 261 200 197 136 88
85 69 18
BspEI T'CCGG_A
Cuts at: 8373 8373
Size: 8710
BspHI T'CATG_A
Cuts at: 6599 8329 6599
Size: 1730 6980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 3904 6065 844
Size: 1444 1616 2161 3489
Fragments arranged by size:
3489 2161 1616 1444
BsrBI CCG'CTC
Cuts at: 1394 1823 2420 6597 1394
Size: 429 597 4177 3507
Fragments arranged by size:
4177 3507 597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3727 5901 7205 7379 65
Size: 1183 2479 2174 1304 174 1396
Fragments arranged by size:
2479 2174 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2307 4975 7354 7541 1700
Size: 110 497 2668 2379 187 2869
Fragments arranged by size:
2869 2668 2379 497 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8710
BssSI C'ACGA_G
Cuts at: 4592 6770 7782 4592
Size: 2178 1012 5520
Fragments arranged by size:
5520 2178 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8710
BstBI TT'CG_AA
Cuts at: 2007 7514 2007
Size: 5507 3203
BstEII G'GTnAC_C
Cuts at: 1184 3673 4360 1184
Size: 2489 687 5534
Fragments arranged by size:
5534 2489 687
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2867 2989 4172 4357 4627 4790 4933 2029
Size: 838 122 1183 185 270 163 143 5806
Fragments arranged by size:
5806 1183 838 270 185 163 143 122
BstYI r'GATC_y
Cuts at: 1972 2232 2856 5039 5385 5454 6040 6407 6792
Size: 260 624 2183 346 69 586 367 385
Cuts at: 6792 6809 8261 8347 8359 8705 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2183 1977 1452 624 586 385 367 346
346 260 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2568 2568
Size: 8710
BtgI C'CryG_G
Cuts at: 513 1065 2452 3806 4907 4941 4981 5220 513
Size: 552 1387 1354 1101 34 40 239 4003
Fragments arranged by size:
4003 1387 1354 1101 552 239 40 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3470 487
Size: 683 56 2244 5727
Fragments arranged by size:
5727 2244 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 3970 5370 6226 7020 7040 3496
Size: 474 1400 856 794 20 5166
Fragments arranged by size:
5166 1400 856 794 474 20
ClaI AT'CG_AT
Cuts at: 1554 3108 1554
Size: 1554 7156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8675
DraI TTT'AAA
Cuts at: 2488 6014 2488
Size: 3526 5184
DraIII CAC_nnn'GTG
Cuts at: 2622 2622
Size: 8710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2335 4840 7717 809
Size: 507 1019 2505 2877 1802
Fragments arranged by size:
2877 2505 1802 1019 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2538 3272 6157
Size: 54 1425 50 275 18 708 734 2885
Cuts at: 6157 7046 8675 8
Size: 889 1629 43
Fragments arranged by size:
2885 1629 1425 889 734 708 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6157 1537
Size: 275 4345 4090
Fragments arranged by size:
4345 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 3689 3732 4180 4655 5253 5884 5907
Size: 1074 1262 43 448 475 598 631 23
Cuts at: 5907 6640 1353
Size: 733 3423
Fragments arranged by size:
3423 1262 1074 733 631 598 475 448
43 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2225 7297 7671 7817 387
Size: 1064 138 636 5072 374 146 1280
Fragments arranged by size:
5072 1280 1064 636 374 146 138
EcoICRI GAG'CTC
Cuts at: 4591 4591
Size: 8710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2228 2666 2736 3296 3359 3521
Size: 393 449 147 438 70 560 63 162
Cuts at: 3521 3890 4319 5234 5471 5522 5669 5747 6773
Size: 369 429 915 237 51 147 78 1026
Cuts at: 6773 7370 8157 8425 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1026 915 787 597 560 449 438
429 393 369 268 237 162 147 147
78 70 63 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3199 4715 5291 3199
Size: 1516 576 6618
Fragments arranged by size:
6618 1516 576
EcoO109I rG'GnC_Cy
Cuts at: 2140 3435 4461 4640 5549 5747 2140
Size: 1295 1026 179 909 198 5103
Fragments arranged by size:
5103 1295 1026 909 198 179
EcoRI G'AATT_C
Cuts at: 4604 6019 6397 4604
Size: 1415 378 6917
Fragments arranged by size:
6917 1415 378
EcoRV GAT'ATC
Cuts at: 2187 5701 2187
Size: 3514 5196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 4351 2410
Size: 1941 6769
FspI TGC'GCA
Cuts at: 7216 7216
Size: 8710
HaeII r_GCGC'y
Cuts at: 1678 3942 7857 1678
Size: 2264 3915 2531
Fragments arranged by size:
3915 2531 2264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2404 2436 2677
Size: 32 135 32 514 32 286 32 241
Cuts at: 2677 2709 2752 2784 2862 2894 3104 3136 3387
Size: 32 43 32 78 32 210 32 251
Cuts at: 3387 3419 3621 3653 3880 3912 4962 4994 5613
Size: 32 202 32 227 32 1050 32 619
Cuts at: 5613 5645 5690 5722 7351 7383 7425 7457 1373
Size: 32 45 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1629 1050 619 514 286 251 241
227 210 202 135 78 45 43 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 3418 6054 6300 669
Size: 988 281 502 978 2636 246 3079
Fragments arranged by size:
3079 2636 988 978 502 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8710
HpaI GTT'AAC
Cuts at: 2440 6300 2440
Size: 3860 4850
KasI G'GCGC_C
Cuts at: 3938 3938
Size: 8710
KpnI G_GTAC'C
Cuts at: 6035 6035
Size: 8710
MfeI C'AATT_G
Cuts at: 4387 6309 4387
Size: 1922 6788
MluI A'CGCG_T
Cuts at: 8586 8586
Size: 8710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 2343 3605 4162 4771 7824 8008 1305
Size: 846 192 1262 557 609 3053 184 2007
Fragments arranged by size:
3053 2007 1262 846 609 557 192 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2540 3274 10
Size: 54 1425 343 708 734 5446
Fragments arranged by size:
5446 1425 734 708 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 3203 3489 4508 5024 6668 7027
Size: 648 927 1110 286 1019 516 1644 359
Cuts at: 7027 7186 518
Size: 159 2042
Fragments arranged by size:
2042 1644 1110 1019 927 648 516 359
286 159
MspA1I CmG'CkG
Cuts at: 1606 4242 4297 4539 4769 5106 5670 6126 6803
Size: 2636 55 242 230 337 564 456 677
Cuts at: 6803 7951 8196 1606
Size: 1148 245 2120
Fragments arranged by size:
2636 2120 1148 677 564 456 337 245
242 230 55
NarI GG'CG_CC
Cuts at: 3939 3939
Size: 8710
NcoI C'CATG_G
Cuts at: 513 1065 2452 4907 4941 4981 5220 513
Size: 552 1387 2455 34 40 239 4003
Fragments arranged by size:
4003 2455 1387 552 239 40 34
NdeI CA'TA_TG
Cuts at: 387 4410 387
Size: 4023 4687
NheI G'CTAG_C
Cuts at: 1052 5830 1052
Size: 4778 3932
NotI GC'GGCC_GC
Cuts at: 6157 6157
Size: 8710
NspI r_CATG'y
Cuts at: 1132 1229 6068 7613 1132
Size: 97 4839 1545 2229
Fragments arranged by size:
4839 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8710
PciI A'CATG_T
Cuts at: 7609 7609
Size: 8710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2397 2622 2753 2964 3038 4213 5841 1108
Size: 1289 225 131 211 74 1175 1628 3977
Fragments arranged by size:
3977 1628 1289 1175 225 211 131 74
PmeI GTTT'AAAC
Cuts at: 6014 6014
Size: 8710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4080 4112 4716 4748 6779 6811 8292 8324 4080
Size: 32 604 32 2031 32 1481 32 4466
Fragments arranged by size:
4466 2031 1481 604 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3435 4461 4640 5549 3435
Size: 1026 179 909 6596
Fragments arranged by size:
6596 1026 909 179
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8710
PsiI TTA'TAA
Cuts at: 6280 6280
Size: 8710
PspOMI G'GGCC_C
Cuts at: 2699 2699
Size: 8710
PstI C_TGCA'G
Cuts at: 830 2466 5675 6062 830
Size: 1636 3209 387 3478
Fragments arranged by size:
3478 3209 1636 387
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8710
PvuII CAG'CTG
Cuts at: 4242 4539 4769 5106 5670 4242
Size: 297 230 337 564 7282
Fragments arranged by size:
7282 564 337 297 230
SacI G_AGCT'C
Cuts at: 4593 4593
Size: 8710
SalI G'TCGA_C
Cuts at: 1936 6052 1936
Size: 4116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2427 3689 2427
Size: 1262 7448
SbfI CC_TGCA'GG
Cuts at: 6062 6062
Size: 8710
ScaI AGT'ACT
Cuts at: 3336 6958 8670 3336
Size: 3622 1712 3376
Fragments arranged by size:
3622 3376 1712
SfcI C'TryA_G
Cuts at: 826 1045 2462 3145 3163 3439 4813 5389 5665
Size: 219 1417 683 18 276 1374 576 276
Cuts at: 5665 5671 5769 6058 7193 7874 8065 826
Size: 6 98 289 1135 681 191 1471
Fragments arranged by size:
1471 1417 1374 1135 683 681 576 289
276 276 219 191 98 18 6
SfoI GGC'GCC
Cuts at: 3940 3940
Size: 8710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8710
SmaI CCC'GGG
Cuts at: 1872 6037 1872
Size: 4165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3666 4019 6814 7715 7977 820
Size: 197 926 1723 353 2795 901 262 1553
Fragments arranged by size:
2795 1723 1553 926 901 353 262 197
SnaBI TAC'GTA
Cuts at: 493 6007 6501 493
Size: 5514 494 2702
Fragments arranged by size:
5514 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8710
SphI G_CATG'C
Cuts at: 1132 6068 1132
Size: 4936 3774
SspI AAT'ATT
Cuts at: 5 52 6634 5
Size: 47 6582 2081
Fragments arranged by size:
6582 2081 47
StuI AGG'CCT
Cuts at: 1517 5690 1517
Size: 4173 4537
StyI C'CwwG_G
Cuts at: 513 1065 2144 2452 2740 3934 4681 4907 4941
Size: 552 1079 308 288 1194 747 226 34
Cuts at: 4941 4981 5220 5817 6149 513
Size: 40 239 597 332 3074
Fragments arranged by size:
3074 1194 1079 747 597 552 332 308
288 239 226 40 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2418 4873 5449 6737 6754 6907 7092
Size: 1255 838 2455 576 1288 17 153 185
Cuts at: 7092 7537 325
Size: 445 1498
Fragments arranged by size:
2455 1498 1288 1255 838 576 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3334 6956 8668
Size: 274 80 33 51 1443 1357 3622 1712
Cuts at: 8668 96
Size: 138
Fragments arranged by size:
3622 1712 1443 1357 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5429 5461 8670 8702 5429
Size: 32 3209 32 5437
Fragments arranged by size:
5437 3209 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5627 1316
Size: 4311 4399
XbaI T'CTAG_A
Cuts at: 2356 5575 6046 2356
Size: 3219 471 5020
Fragments arranged by size:
5020 3219 471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 3796 4294 4582 2525
Size: 1271 498 288 6653
Fragments arranged by size:
6653 1271 498 288
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8710
XmaI C'CCGG_G
Cuts at: 1870 6035 1870
Size: 4165 4545
XmnI GAAnn'nnTTC
Cuts at: 2066 3838 5571 6839 2066
Size: 1772 1733 1268 3937
Fragments arranged by size:
3937 1772 1733 1268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6518 276
Size: 53 83 186 936 4984 2468
Fragments arranged by size:
4984 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI