Restriction map for
pFN21ASDA0362
(Circular) (Six-base) MAPSORT of: pFN21ASDA0362.seq Check: 2867 from: 1 to: 8017
pFN21ASDA0362 8017 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 3207 2189
Size: 1018 6999
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2823 3431 5827 278
Size: 53 83 186 936 1287 608 2396 2468
Fragments arranged by size:
2468 2396 1287 936 608 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 3309 4797 5360 1937
Size: 891 481 1488 563 4594
Fragments arranged by size:
4594 1488 891 563 481
Acc65I G'GTAC_C
Cuts at: 5338 5338
Size: 8017
AclI AA'CG_TT
Cuts at: 1563 6144 6517 1563
Size: 4581 373 3063
Fragments arranged by size:
4581 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2323 3713 4346 4466 6080 7464 7732 1632
Size: 691 1390 633 120 1614 1384 268 1917
Fragments arranged by size:
1917 1614 1390 1384 691 633 268 120
AfeI AGC'GCT
Cuts at: 2267 2267
Size: 8017
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7820
AflIII A'CryG_T
Cuts at: 1203 6916 7893 1203
Size: 5713 977 1327
Fragments arranged by size:
5713 1327 977
AgeI A'CCGG_T
Cuts at: 3545 6848 3545
Size: 3303 4714
AhdI GACnn_n'nnGTC
Cuts at: 2824 3139 6746 2824
Size: 315 3607 4095
Fragments arranged by size:
4095 3607 315
AleI CACnn'nnGTG
Cuts at: 1166 3348 4574 5216 1166
Size: 2182 1226 642 3967
Fragments arranged by size:
3967 2182 1226 642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8017
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 3653 3685 3794 3826 2345
Size: 32 1276 32 109 32 6536
Fragments arranged by size:
6536 1276 109 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2773 3070 3082 4360 4392 4545 4927 5760 7332
Size: 297 12 1278 32 153 382 833 1572
Cuts at: 7332 2773
Size: 3458
Fragments arranged by size:
3458 1572 1278 833 382 297 153 32
12
ApaI G_GGCC'C
Cuts at: 3256 3262 3840 5186 3256
Size: 6 578 1346 6087
Fragments arranged by size:
6087 1346 578 6
ApaLI G'TGCA_C
Cuts at: 6074 7230 6074
Size: 1156 6861
ApoI r'AATT_y
Cuts at: 1493 2576 4848 5326 5552 5704 7813 1493
Size: 1083 2272 478 226 152 2109 1697
Fragments arranged by size:
2272 2109 1697 1083 478 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6571 160
Size: 872 5539 1606
Fragments arranged by size:
5539 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
AvaI C'yCGr_G
Cuts at: 1870 1943 3255 3746 5342 1870
Size: 73 1312 491 1596 4545
Fragments arranged by size:
4545 1596 1312 491 73
BamHI G'GATC_C
Cuts at: 5347 5714 5347
Size: 367 7650
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2605 3342 4611 4810 5104
Size: 325 217 627 818 737 1269 199 294
Cuts at: 5104 5338 6793 618
Size: 234 1455 1842
Fragments arranged by size:
1842 1455 1269 818 737 627 325 294
234 217 199
BanII G_rGCy'C
Cuts at: 1391 2627 2951 2958 3256 3262 3339 3573 3840
Size: 1236 324 7 298 6 77 234 267
Cuts at: 3840 3948 4430 4535 5186 5336 1391
Size: 108 482 105 651 150 4072
Fragments arranged by size:
4072 1236 651 482 324 298 267 234
150 108 105 77 7 6
BbeI G_GCGC'C
Cuts at: 4814 4814
Size: 8017
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3631 928
Size: 971 1732 5314
Fragments arranged by size:
5314 1732 971
BbvCI CC'TCA_GC
Cuts at: 2943 3203 4404 2943
Size: 260 1201 6556
Fragments arranged by size:
6556 1201 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2713 2747 3803 3837 6208 6242 1903
Size: 34 776 34 1056 34 2371 34 3678
Fragments arranged by size:
3678 2371 1056 776 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5021 5909 7119 5021
Size: 888 1210 5919
Fragments arranged by size:
5919 1210 888
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3879 6628 136
Size: 107 122 71 631 605 2207 2749 1525
Fragments arranged by size:
2749 2207 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3613 4197 4770 8012 3613
Size: 584 573 3242 3618
Fragments arranged by size:
3618 3242 584 573
BlpI GC'TnA_GC
Cuts at: 2621 3392 3889 4304 4424 5038 5434 2621
Size: 771 497 415 120 614 396 5204
Fragments arranged by size:
5204 771 614 497 415 396 120
Bme1580I G_kGCm'C
Cuts at: 1790 2910 3256 3262 3840 5186 6078 7234 1790
Size: 1120 346 6 578 1346 892 1156 2573
Fragments arranged by size:
2573 1346 1156 1120 892 578 346 6
BmgBI CAC'GTC
Cuts at: 1318 5272 1318
Size: 3954 4063
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 5748 6706
Size: 416 53 83 399 24 1535 2796 958
Cuts at: 6706 442
Size: 1753
Fragments arranged by size:
2796 1753 1535 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2939 3092 3425 3443 3503 3790 4013 4196
Size: 1700 153 333 18 60 287 223 183
Cuts at: 4196 4931 5042 5120 6677 1239
Size: 735 111 78 1557 2579
Fragments arranged by size:
2579 1700 1557 735 333 287 223 183
153 111 78 60 18
Bpu10I CC'TnA_GC
Cuts at: 2943 3203 4404 5015 2943
Size: 260 1201 611 5945
Fragments arranged by size:
5945 1201 611 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4349 4945 4957 6142 7007 7305 4349
Size: 596 12 1185 865 298 5061
Fragments arranged by size:
5061 1185 865 596 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3536 6680 882
Size: 617 2037 3144 2219
Fragments arranged by size:
3144 2219 2037 617
BsaAI yAC'GTr
Cuts at: 493 1204 3628 3934 3970 5314 5808 493
Size: 711 2424 306 36 1344 494 2702
Fragments arranged by size:
2702 2424 1344 711 494 306 36
BsaBI GATnn'nnATC
Cuts at: 8011 8011
Size: 8017
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2471 2820 3428 4811
Size: 53 83 186 936 938 349 608 1383
Cuts at: 4811 5824 6206 6841 275
Size: 1013 382 635 1451
Fragments arranged by size:
1451 1383 1013 938 936 635 608 382
349 186 83 53
BsaWI w'CCGG_w
Cuts at: 2282 2350 3545 3828 3843 6450 6848 7122 7269
Size: 68 1195 283 15 2607 398 274 147
Cuts at: 7269 7680 2282
Size: 411 2619
Fragments arranged by size:
2619 2607 1195 411 398 283 274 147
68 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2687 2717 3653 3683 3794
Size: 30 165 30 1284 30 936 30 111
Cuts at: 3794 3824 3968 3998 1178
Size: 30 144 30 5197
Fragments arranged by size:
5197 1284 936 165 144 111 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3239 3394 3677 3914 3917 3989 4673 4805
Size: 2053 155 283 237 3 72 684 132
Cuts at: 4805 5153 5180 5275 1186
Size: 348 27 95 3928
Fragments arranged by size:
3928 2053 684 348 283 237 155 132
95 72 27 3
BseYI C'CCAG_C
Cuts at: 2081 2149 2396 3012 7220 7716 2081
Size: 68 247 616 4208 496 2382
Fragments arranged by size:
4208 2382 616 496 247 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3017 3395 3746 4355 4607 5154 5306
Size: 573 1281 378 351 609 252 547 152
Cuts at: 5306 1163
Size: 3874
Fragments arranged by size:
3874 1281 609 573 547 378 351 252
152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 5217 5467 6228 7256 1540
Size: 275 186 195 3021 250 761 1028 2301
Fragments arranged by size:
3021 2301 1028 761 275 250 195 186
BsiHKAI G_wGCw'C
Cuts at: 2627 3198 3339 3573 3627 3948 4256 4430 4641
Size: 571 141 234 54 321 308 174 211
Cuts at: 4641 6078 6163 7234 2627
Size: 1437 85 1071 3410
Fragments arranged by size:
3410 1437 1071 571 321 308 234 211
174 141 85 54
BsmI GAATG_Cn'
Cuts at: 3783 4283 5528 5621 3783
Size: 500 1245 93 6179
Fragments arranged by size:
6179 1245 500 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3419 3419
Size: 8017
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2627 2910 2951 2958 3198 3256 3262
Size: 399 837 283 41 7 240 58 6
Cuts at: 3262 3339 3573 3627 3840 3948 4256 4430 4535
Size: 77 234 54 213 108 308 174 105
Cuts at: 4535 4641 5186 5336 6078 6163 7234 1391
Size: 106 545 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 837 742 545 399 308 283
240 234 213 174 150 108 106 105
85 77 58 54 41 7 6
BspEI T'CCGG_A
Cuts at: 2350 3828 7680 2350
Size: 1478 3852 2687
Fragments arranged by size:
3852 2687 1478
BspHI T'CATG_A
Cuts at: 2373 5906 7636 2373
Size: 3533 1730 2754
Fragments arranged by size:
3533 2754 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2189 2517 3207 3468 4329 4419 5372
Size: 1261 84 328 690 261 861 90 953
Cuts at: 5372 844
Size: 3489
Fragments arranged by size:
3489 1261 953 861 690 328 261 90
84
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 4230 5265 5904 1394
Size: 429 407 2000 1035 639 3507
Fragments arranged by size:
3507 2000 1035 639 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 6512 6686 65
Size: 1183 5264 174 1396
Fragments arranged by size:
5264 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2439 2608 3545 3730 3873 4484 4936
Size: 110 629 169 937 185 143 611 452
Cuts at: 4936 6661 6848 1700
Size: 1725 187 2869
Fragments arranged by size:
2869 1725 937 629 611 452 187 185
169 143 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8017
BssHII G'CGCG_C
Cuts at: 1925 2145 4784 1925
Size: 220 2639 5158
Fragments arranged by size:
5158 2639 220
BssSI C'ACGA_G
Cuts at: 2522 4625 5228 6077 7089 2522
Size: 2103 603 849 1012 3450
Fragments arranged by size:
3450 2103 1012 849 603
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3082 4392 794
Size: 2288 1310 4419
Fragments arranged by size:
4419 2288 1310
BstBI TT'CG_AA
Cuts at: 2007 6821 2007
Size: 4814 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8017
BstXI CCAn_nnnn'nTGG
Cuts at: 5194 5194
Size: 8017
BstYI r'GATC_y
Cuts at: 1972 3561 3613 4197 4770 5347 5714 6099 6116
Size: 1589 52 584 573 577 367 385 17
Cuts at: 6116 7568 7654 7666 8012 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1589 1452 584 577 573 385 367
346 86 52 17 12
Bsu36I CC'TnA_GG
Cuts at: 2927 4121 5109 2927
Size: 1194 988 5835
Fragments arranged by size:
5835 1194 988
BtgI C'CryG_G
Cuts at: 513 1065 2062 2090 2185 3243 3883 4005 513
Size: 552 997 28 95 1058 640 122 4525
Fragments arranged by size:
4525 1058 997 640 552 122 95 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2470 2551 4459 487
Size: 683 56 1244 81 1908 4045
Fragments arranged by size:
4045 1908 1244 683 81 56
BtsI GCAGTG_nn'
Cuts at: 3083 4641 5533 6327 6347 3083
Size: 1558 892 794 20 4753
Fragments arranged by size:
4753 1558 892 794 20
ClaI AT'CG_AT
Cuts at: 1554 2652 1554
Size: 1098 6919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7982
DraI TTT'AAA
Cuts at: 5321 5321
Size: 8017
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7024 809
Size: 507 5708 1802
Fragments arranged by size:
5708 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2129 2193
Size: 54 1425 50 275 18 225 74 64
Cuts at: 2193 2727 3213 3240 3705 3915 4587 5464 6353
Size: 534 486 27 465 210 672 877 889
Cuts at: 6353 7982 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 877 672 534 486 465
275 225 210 74 64 54 50 43
27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2193 5464 1537
Size: 275 381 3271 4090
Fragments arranged by size:
4090 3271 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4646 5947 1353
Size: 3293 1301 3423
Fragments arranged by size:
3423 3293 1301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3308 5237 6604 6978 7124 387
Size: 1064 138 1719 1929 1367 374 146 1280
Fragments arranged by size:
1929 1719 1367 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2625 3337 3571 3946 4428 2625
Size: 712 234 375 482 6214
Fragments arranged by size:
6214 712 482 375 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2323 2939 3092 3425 3443 3503 3713
Size: 393 691 616 153 333 18 60 210
Cuts at: 3713 3790 4013 4196 4346 4466 4931 5042 5120
Size: 77 223 183 150 120 465 111 78
Cuts at: 5120 6080 6677 7464 7732 1239
Size: 960 597 787 268 1524
Fragments arranged by size:
1524 960 787 691 616 597 465 393
333 268 223 210 183 153 150 120
111 78 77 60 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3844 4415 3844
Size: 571 7446
EcoO109I rG'GnC_Cy
Cuts at: 2674 2685 3208 3252 3259 3836 3837 4408 5183
Size: 11 523 44 7 577 1 571 775
Cuts at: 5183 5240 5302 2674
Size: 57 62 5389
Fragments arranged by size:
5389 775 577 571 523 62 57 44
11 7 1
EcoRI G'AATT_C
Cuts at: 5326 5704 5326
Size: 378 7639
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 4726 2284
Size: 2442 5575
FspI TGC'GCA
Cuts at: 2483 2850 3926 6523 2483
Size: 367 1076 2597 3977
Fragments arranged by size:
3977 2597 1076 367
FspAI rTGC'GCAy
Cuts at: 2850 2850
Size: 8017
HaeII r_GCGC'y
Cuts at: 1678 2269 4424 4814 4910 5045 7164 1678
Size: 591 2155 390 96 135 2119 2531
Fragments arranged by size:
2531 2155 2119 591 390 135 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 2810 2842 3125
Size: 32 135 32 618 32 588 32 283
Cuts at: 3125 3157 3215 3247 3344 3376 6658 6690 6732
Size: 32 58 32 97 32 3282 32 42
Cuts at: 6732 6764 1373
Size: 32 2626
Fragments arranged by size:
3282 2626 618 588 283 135 97 58
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 4044 5361 5607 669
Size: 988 281 891 1215 1317 246 3079
Fragments arranged by size:
3079 1317 1215 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8017
HpaI GTT'AAC
Cuts at: 5607 5607
Size: 8017
KasI G'GCGC_C
Cuts at: 4810 4810
Size: 8017
KpnI G_GTAC'C
Cuts at: 5342 5342
Size: 8017
MfeI C'AATT_G
Cuts at: 5616 5616
Size: 8017
MluI A'CGCG_T
Cuts at: 7893 7893
Size: 8017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 4141 4281 5057 5181 7131 7315 1305
Size: 1281 1555 140 776 124 1950 184 2007
Fragments arranged by size:
2007 1950 1555 1281 776 184 140 124
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3215 3917 4589 10
Size: 54 1425 343 1383 702 672 3438
Fragments arranged by size:
3438 1425 1383 702 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 3348 4574 5216 5975 6334 6493
Size: 648 1263 919 1226 642 759 359 159
Cuts at: 6493 518
Size: 2042
Fragments arranged by size:
2042 1263 1226 919 759 648 642 359
159
MspA1I CmG'CkG
Cuts at: 1606 2665 3016 3885 3893 4007 4357 4609 4873
Size: 1059 351 869 8 114 350 252 264
Cuts at: 4873 5433 6110 7258 7503 1606
Size: 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 1059 869 677 560 351 350
264 252 245 114 8
NaeI GCC'GGC
Cuts at: 3875 4938 3875
Size: 1063 6954
NarI GG'CG_CC
Cuts at: 4811 4811
Size: 8017
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8017
NgoMIV G'CCGG_C
Cuts at: 3873 4936 3873
Size: 1063 6954
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8017
NotI GC'GGCC_GC
Cuts at: 5464 5464
Size: 8017
NspI r_CATG'y
Cuts at: 1132 1229 2128 3191 5375 6920 1132
Size: 97 899 1063 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 1063 899 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8017
PciI A'CATG_T
Cuts at: 6916 6916
Size: 8017
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3438 5098 1108
Size: 2330 1660 4027
Fragments arranged by size:
4027 2330 1660
PfoI T'CCnGG_A
Cuts at: 2247 2916 3111 3519 3564 3948 3990 4272 2247
Size: 669 195 408 45 384 42 282 5992
Fragments arranged by size:
5992 669 408 384 282 195 45 42
PmeI GTTT'AAAC
Cuts at: 5321 5321
Size: 8017
PmlI CAC'GTG
Cuts at: 3628 3934 3628
Size: 306 7711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3794 3826 6086 6118 7599 7631 3653
Size: 32 109 32 2260 32 1481 32 4039
Fragments arranged by size:
4039 2260 1481 109 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2685 3208 4408 2674
Size: 11 523 1200 6283
Fragments arranged by size:
6283 1200 523 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8017
PsiI TTA'TAA
Cuts at: 5587 5587
Size: 8017
PspOMI G'GGCC_C
Cuts at: 3252 3258 3836 5182 3252
Size: 6 578 1346 6087
Fragments arranged by size:
6087 1346 578 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 7985
PstI C_TGCA'G
Cuts at: 830 2171 2406 2514 2778 2940 3087 4416 4878
Size: 1341 235 108 264 162 147 1329 462
Cuts at: 4878 5369 830
Size: 491 3478
Fragments arranged by size:
3478 1341 1329 491 462 264 235 162
147 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8017
PvuII CAG'CTG
Cuts at: 2665 3016 4357 4873 2665
Size: 351 1341 516 5809
Fragments arranged by size:
5809 1341 516 351
SacI G_AGCT'C
Cuts at: 2627 3339 3573 3948 4430 2627
Size: 712 234 375 482 6214
Fragments arranged by size:
6214 712 482 375 234
SacII CC_GC'GG
Cuts at: 3886 4008 3886
Size: 122 7895
SalI G'TCGA_C
Cuts at: 1936 2827 5359 1936
Size: 891 2532 4594
Fragments arranged by size:
4594 2532 891
SanDI GG'GwC_CC
Cuts at: 2685 2685
Size: 8017
SapI GCTCTTCn'nnn_
Cuts at: 4646 4646
Size: 8017
SbfI CC_TGCA'GG
Cuts at: 2406 4416 5369 2406
Size: 2010 953 5054
Fragments arranged by size:
5054 2010 953
ScaI AGT'ACT
Cuts at: 4211 6265 7977 4211
Size: 2054 1712 4251
Fragments arranged by size:
4251 2054 1712
SfcI C'TryA_G
Cuts at: 826 1045 2167 2402 2510 2774 2936 3083 4412
Size: 219 1122 235 108 264 162 147 1329
Cuts at: 4412 4693 4874 5365 6500 7181 7372 826
Size: 281 181 491 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 1122 681 491 281 264
235 219 191 181 162 147 108
SfoI GGC'GCC
Cuts at: 4812 4812
Size: 8017
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8017
SmaI CCC'GGG
Cuts at: 1872 3257 5344 1872
Size: 1385 2087 4545
Fragments arranged by size:
4545 2087 1385
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4364 4960 4972 6121 7022 7284
Size: 197 926 2421 596 12 1149 901 262
Cuts at: 7284 820
Size: 1553
Fragments arranged by size:
2421 1553 1149 926 901 596 262 197
12
SnaBI TAC'GTA
Cuts at: 493 5314 5808 493
Size: 4821 494 2702
Fragments arranged by size:
4821 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8017
SphI G_CATG'C
Cuts at: 1132 2128 5375 1132
Size: 996 3247 3774
Fragments arranged by size:
3774 3247 996
SrfI GCCC'GGGC
Cuts at: 3257 3257
Size: 8017
SspI AAT'ATT
Cuts at: 5 52 5941 5
Size: 47 5889 2081
Fragments arranged by size:
5889 2081 47
StuI AGG'CCT
Cuts at: 1517 4050 1517
Size: 2533 5484
StyI C'CwwG_G
Cuts at: 513 1065 4572 5456 513
Size: 552 3507 884 3074
Fragments arranged by size:
3507 3074 884 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 2684 2804 4565 4828 6044 6061
Size: 1255 1089 15 120 1761 263 1216 17
Cuts at: 6061 6214 6399 6844 325
Size: 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 1216 1089 445 263 185
153 120 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4209 6263 7975
Size: 274 80 33 51 1443 2232 2054 1712
Cuts at: 7975 96
Size: 138
Fragments arranged by size:
2232 2054 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 7977 8009 3968
Size: 32 3977 32 3976
Fragments arranged by size:
3977 3976 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 1316
Size: 906 7111
XbaI T'CTAG_A
Cuts at: 5353 5353
Size: 8017
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8017
XmaI C'CCGG_G
Cuts at: 1870 3255 5342 1870
Size: 1385 2087 4545
Fragments arranged by size:
4545 2087 1385
XmnI GAAnn'nnTTC
Cuts at: 6146 6146
Size: 8017
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2821 3429 5825 276
Size: 53 83 186 936 1287 608 2396 2468
Fragments arranged by size:
2468 2396 1287 936 608 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII
EcoRV FseI NruI NsiI PacI PspXI RsrII SexAI
SfiI SwaI XcmI