Restriction map for
pFN21ASDA0341
(Circular) (Six-base) MAPSORT of: pFN21ASDA0341.seq Check: 8534 from: 1 to: 6361
pFN21ASDA0341 6361 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2863 2863
Size: 6361
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4171 278
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3704 1937
Size: 1767 4594
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 2296 2629 2933 3202 3245 4424 5808
Size: 489 175 333 304 269 43 1179 1384
Cuts at: 5808 6076 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1179 489 333 304 269 268
175 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 3561 5260 6237 1203
Size: 2358 1699 977 1327
Fragments arranged by size:
2358 1699 1327 977
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 5090 5090
Size: 6361
AleI CACnn'nnGTG
Cuts at: 1166 2873 1166
Size: 1707 4654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3047 3392 1184
Size: 1863 345 4153
Fragments arranged by size:
4153 1863 345
AlwNI CAG_nnn'CTG
Cuts at: 2042 2446 2536 2878 2896 3049 3089 3172 3205
Size: 404 90 342 18 153 40 83 33
Cuts at: 3205 3472 3583 3595 4104 5676 2042
Size: 267 111 12 509 1572 2727
Fragments arranged by size:
2727 1572 509 404 342 267 153 111
90 83 40 33 18 12
ApaI G_GGCC'C
Cuts at: 2248 2508 3070 2248
Size: 260 562 5539
Fragments arranged by size:
5539 562 260
ApaLI G'TGCA_C
Cuts at: 3099 4418 5574 3099
Size: 1319 1156 3886
Fragments arranged by size:
3886 1319 1156
ApoI r'AATT_y
Cuts at: 1493 3670 3896 4048 6157 1493
Size: 2177 226 152 2109 1697
Fragments arranged by size:
2177 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 2501 2508 2523 2573 2675 2747
Size: 73 187 371 7 15 50 102 72
Cuts at: 2747 2810 3063 3324 3638 3686 1870
Size: 63 253 261 314 48 4545
Fragments arranged by size:
4545 371 314 261 253 187 102 73
72 63 50 48 15 7
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3015 3074 3682 5137 618
Size: 325 217 627 1228 59 608 1455 1842
Fragments arranged by size:
1842 1455 1228 627 608 325 217 59
BanII G_rGCy'C
Cuts at: 1391 2138 2157 2248 2501 2508 2574 2580 2684
Size: 747 19 91 253 7 66 6 104
Cuts at: 2684 2829 3040 3070 3615 3680 1391
Size: 145 211 30 545 65 4072
Fragments arranged by size:
4072 747 545 253 211 145 104 91
66 65 30 19 7 6
BbeI G_GCGC'C
Cuts at: 3019 3019
Size: 6361
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3146 3233 928
Size: 971 1247 87 4056
Fragments arranged by size:
4056 1247 971 87
BbvCI CC'TCA_GC
Cuts at: 2189 2546 2189
Size: 357 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3305 3339 4552 4586 1903
Size: 34 1368 34 1213 34 3678
Fragments arranged by size:
3678 1368 1213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2046 2152 2519
Size: 107 122 71 631 605 374 106 367
Cuts at: 2519 4972 136
Size: 2453 1525
Fragments arranged by size:
2453 1525 631 605 374 367 122 107
106 71
BglII A'GATC_T
Cuts at: 3213 3651 6356 3213
Size: 438 2705 3218
Fragments arranged by size:
3218 2705 438
BlpI GC'TnA_GC
Cuts at: 2735 2907 2958 3000 3039 3417 3432 3778 2735
Size: 172 51 42 39 378 15 346 5318
Fragments arranged by size:
5318 378 346 172 51 42 39 15
Bme1580I G_kGCm'C
Cuts at: 1790 2248 2508 2530 3070 3103 3253 3604 4422
Size: 458 260 22 540 33 150 351 818
Cuts at: 4422 5578 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 818 540 458 351 260 150
33 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3090 4092 5050
Size: 416 53 83 399 24 1673 1002 958
Cuts at: 5050 442
Size: 1753
Fragments arranged by size:
1753 1673 1002 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2581 2900 2963 3605 3646 5021 1239
Size: 1342 319 63 642 41 1375 2579
Fragments arranged by size:
2579 1375 1342 642 319 63 41
Bpu10I CC'TnA_GC
Cuts at: 2189 2495 2546 2568 3264 2189
Size: 306 51 22 696 5286
Fragments arranged by size:
5286 696 306 51 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2685 2813 4486 5351 5649 2685
Size: 128 1673 865 298 3397
Fragments arranged by size:
3397 1673 865 298 128
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2133 2807 5024 882
Size: 617 634 674 2217 2219
Fragments arranged by size:
2219 2217 674 634 617
BsaAI yAC'GTr
Cuts at: 493 1204 3658 4152 493
Size: 711 2454 494 2702
Fragments arranged by size:
2702 2454 711 494
BsaBI GATnn'nnATC
Cuts at: 6355 6355
Size: 6361
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2426 3016 4168 4550
Size: 53 83 186 936 893 590 1152 382
Cuts at: 4550 5185 275
Size: 635 1451
Fragments arranged by size:
1451 1152 936 893 635 590 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2160 3055 4794 5192 5466 5613 6024 2160
Size: 895 1739 398 274 147 411 2497
Fragments arranged by size:
2497 1739 895 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 1178
Size: 30 165 30 1353 30 4753
Fragments arranged by size:
4753 1353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2584 2641 2770 2837 2897 3125 3521
Size: 961 437 57 129 67 60 228 396
Cuts at: 3521 1186
Size: 4026
Fragments arranged by size:
4026 961 437 396 228 129 67 60
57
BseYI C'CCAG_C
Cuts at: 2579 2717 5564 6060 2579
Size: 138 2847 496 2880
Fragments arranged by size:
2880 2847 496 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2453 2522 2921 1163
Size: 573 447 270 69 399 4603
Fragments arranged by size:
4603 573 447 399 270 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2228 2829 3103 4422 4507 5578 2228
Size: 601 274 1319 85 1071 3011
Fragments arranged by size:
3011 1319 1071 601 274 85
BsmI GAATG_Cn'
Cuts at: 2358 3872 3965 2358
Size: 1514 93 4754
Fragments arranged by size:
4754 1514 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2157 2228 2248 2501 2508 2530
Size: 399 348 19 71 20 253 7 22
Cuts at: 2530 2574 2580 2684 2829 3040 3070 3103 3253
Size: 44 6 104 145 211 30 33 150
Cuts at: 3253 3604 3615 3680 4422 4507 5578 1391
Size: 351 11 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 348 253 211
150 145 104 85 71 65 44 33
30 22 20 19 11 7 6
BspEI T'CCGG_A
Cuts at: 2160 3055 6024 2160
Size: 895 2969 2497
Fragments arranged by size:
2969 2497 895
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 2863 3716 844
Size: 1361 658 853 3489
Fragments arranged by size:
3489 1361 853 658
BsrBI CCG'CTC
Cuts at: 1394 1823 2932 3490 4248 1394
Size: 429 1109 558 758 3507
Fragments arranged by size:
3507 1109 758 558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2049 2464 4856 5030 65
Size: 1183 801 415 2392 174 1396
Fragments arranged by size:
2392 1396 1183 801 415 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6361
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6361
BssSI C'ACGA_G
Cuts at: 2939 4421 5433 2939
Size: 1482 1012 3867
Fragments arranged by size:
3867 1482 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3172 3472 3595 794
Size: 2378 300 123 3560
Fragments arranged by size:
3560 2378 300 123
BstBI TT'CG_AA
Cuts at: 2007 5165 2007
Size: 3158 3203
BstEII G'GTnAC_C
Cuts at: 1184 2944 1184
Size: 1760 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 2150 2150
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 3213 3540 3651 3691 4058 4443 4460 5912
Size: 1241 327 111 40 367 385 17 1452
Cuts at: 5912 5998 6010 6356 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1241 385 367 346 327 111
86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2055 2108 513
Size: 552 957 33 53 4766
Fragments arranged by size:
4766 957 552 53 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2859 487
Size: 683 56 1633 3989
Fragments arranged by size:
3989 1633 683 56
BtsI GCAGTG_nn'
Cuts at: 3602 3877 4671 4691 3602
Size: 275 794 20 5272
Fragments arranged by size:
5272 794 275 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3665 3665
Size: 6361
DraIII CAC_nnn'GTG
Cuts at: 2944 2944
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5368 809
Size: 507 4052 1802
Fragments arranged by size:
4052 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2659 3808
Size: 54 1425 50 275 18 195 634 1149
Cuts at: 3808 4697 6326 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1149 889 634 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 4291 1353
Size: 744 2194 3423
Fragments arranged by size:
3423 2194 744
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3203 3224 3508 4948 5322 5468
Size: 1064 138 1614 21 284 1440 374 146
Cuts at: 5468 387
Size: 1280
Fragments arranged by size:
1614 1440 1280 1064 374 284 146 138
21
EcoICRI GAG'CTC
Cuts at: 2827 2827
Size: 6361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2296 2581 2629 2900 2933 2963
Size: 393 489 175 285 48 271 33 30
Cuts at: 2963 3202 3245 3605 3646 4424 5021 5808 6076
Size: 239 43 360 41 778 597 787 268
Cuts at: 6076 1239
Size: 1524
Fragments arranged by size:
1524 787 778 597 489 393 360 285
271 268 239 175 48 43 41 33
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2555 3023 3083 2162
Size: 393 468 60 5440
Fragments arranged by size:
5440 468 393 60
EcoO109I rG'GnC_Cy
Cuts at: 2039 2245 2505 2705 2836 3046 3067 3319 3373
Size: 206 260 200 131 210 21 252 54
Cuts at: 3373 2039
Size: 5027
Fragments arranged by size:
5027 260 252 210 206 200 131 54
21
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
FspI TGC'GCA
Cuts at: 3023 3302 4867 3023
Size: 279 1565 4517
Fragments arranged by size:
4517 1565 279
HaeII r_GCGC'y
Cuts at: 1678 2890 2920 3019 3473 5508 1678
Size: 1212 30 99 454 2035 2531
Fragments arranged by size:
2531 2035 1212 454 99 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 3205 3237 5002
Size: 32 135 32 1109 32 492 32 1765
Cuts at: 5002 5034 5076 5108 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1765 1109 492 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3705 3951 669
Size: 988 281 1767 246 3079
Fragments arranged by size:
3079 1767 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6361
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KasI G'GCGC_C
Cuts at: 3015 3015
Size: 6361
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 3960 3960
Size: 6361
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2613 2883 5475 5659 1305
Size: 753 555 270 2592 184 2007
Fragments arranged by size:
2592 2007 753 555 270 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2661 10
Size: 54 1425 343 195 634 3710
Fragments arranged by size:
3710 1425 634 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2873 3560 4319 4678 4837 518
Size: 648 888 819 687 759 359 159 2042
Fragments arranged by size:
2042 888 819 759 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2533 2729 2893 2983 2989 3013 3172
Size: 504 423 196 164 90 6 24 159
Cuts at: 3172 3345 3424 3535 3777 4454 5602 5847 1606
Size: 173 79 111 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 504 423 245 242 196
173 164 159 111 90 79 24 6
NarI GG'CG_CC
Cuts at: 3016 3016
Size: 6361
NcoI C'CATG_G
Cuts at: 513 1065 2022 2055 513
Size: 552 957 33 4819
Fragments arranged by size:
4819 957 552 33
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 3565 3719 5264 1132
Size: 97 2336 154 1545 2229
Fragments arranged by size:
2336 2229 1545 154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6361
PciI A'CATG_T
Cuts at: 3561 5260 3561
Size: 1699 4662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3205 3583 3624 1108
Size: 2097 378 41 3845
Fragments arranged by size:
3845 2097 378 41
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 5943 5975 4430
Size: 32 1481 32 4816
Fragments arranged by size:
4816 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2705 2705
Size: 6361
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6361
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PspOMI G'GGCC_C
Cuts at: 2244 2504 3066 2244
Size: 260 562 5539
Fragments arranged by size:
5539 562 260
PspXI vC'TCGA_Gb
Cuts at: 3638 3638
Size: 6361
PstI C_TGCA'G
Cuts at: 830 2988 3312 3444 3713 830
Size: 2158 324 132 269 3478
Fragments arranged by size:
3478 2158 324 269 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 2533 2729 2983 2989 3172 3345 3424 2533
Size: 196 254 6 183 173 79 5470
Fragments arranged by size:
5470 254 196 183 173 79 6
SacI G_AGCT'C
Cuts at: 2829 2829
Size: 6361
SacII CC_GC'GG
Cuts at: 2111 2111
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SapI GCTCTTCn'nnn_
Cuts at: 2097 2097
Size: 6361
SbfI CC_TGCA'GG
Cuts at: 3312 3713 3312
Size: 401 5960
ScaI AGT'ACT
Cuts at: 4609 6321 4609
Size: 1712 4649
SfcI C'TryA_G
Cuts at: 826 1045 2984 3308 3440 3709 4844 5525 5716
Size: 219 1939 324 132 269 1135 681 191
Cuts at: 5716 826
Size: 1471
Fragments arranged by size:
1939 1471 1135 681 324 269 219 191
132
SfiI GGCCn_nnn'nGGCC
Cuts at: 2519 2519
Size: 6361
SfoI GGC'GCC
Cuts at: 3017 3017
Size: 6361
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 2503 2510 2525 3688 1872
Size: 631 7 15 1163 4545
Fragments arranged by size:
4545 1163 631 15 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2700 2828 3638 4465 5366 5628
Size: 197 926 757 128 810 827 901 262
Cuts at: 5628 820
Size: 1553
Fragments arranged by size:
1553 926 901 827 810 757 262 197
128
SnaBI TAC'GTA
Cuts at: 493 3658 4152 493
Size: 3165 494 2702
Fragments arranged by size:
3165 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3719 1132
Size: 2587 3774
SrfI GCCC'GGGC
Cuts at: 2525 2525
Size: 6361
SspI AAT'ATT
Cuts at: 5 52 4285 5
Size: 47 4233 2081
Fragments arranged by size:
4233 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6361
StyI C'CwwG_G
Cuts at: 513 1065 2022 2055 2656 2709 2839 3800 513
Size: 552 957 33 601 53 130 961 3074
Fragments arranged by size:
3074 961 957 601 552 130 53 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4388 4405 4558 4743 5188 325
Size: 1255 2808 17 153 185 445 1498
Fragments arranged by size:
2808 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4607 6319 96
Size: 274 80 33 51 1443 2630 1712 138
Fragments arranged by size:
2630 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6321 6353 6321
Size: 32 6329
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2419 2062
Size: 357 6004
XhoI C'TCGA_G
Cuts at: 1943 3638 1943
Size: 1695 4666
XmaI C'CCGG_G
Cuts at: 1870 2501 2508 2523 3686 1870
Size: 631 7 15 1163 4545
Fragments arranged by size:
4545 1163 631 15 7
XmnI GAAnn'nnTTC
Cuts at: 2402 4490 2402
Size: 2088 4273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4169 276
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI
SexAI SwaI