Restriction map for
pFN21ASDA0332
(Circular) (Six-base) MAPSORT of: pFN21ASDA0332.seq Check: 4191 from: 1 to: 7816
pFN21ASDA0332 7816 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5626 278
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4846 5159 1937
Size: 2909 313 4594
Fragments arranged by size:
4594 2909 313
Acc65I G'GTAC_C
Cuts at: 5137 5137
Size: 7816
AclI AA'CG_TT
Cuts at: 1563 5943 6316 1563
Size: 4380 373 3063
Fragments arranged by size:
4380 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 3744 3753 3822 3967 4275 4476 5879
Size: 453 1659 9 69 145 308 201 1403
Cuts at: 5879 7263 7531 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1659 1403 1384 453 308 268 201
145 69 9
AflII C'TTAA_G
Cuts at: 820 1017 3623 820
Size: 197 2606 5013
Fragments arranged by size:
5013 2606 197
AflIII A'CryG_T
Cuts at: 1203 6715 7692 1203
Size: 5512 977 1327
Fragments arranged by size:
5512 1327 977
AgeI A'CCGG_T
Cuts at: 6647 6647
Size: 7816
AhdI GACnn_n'nnGTC
Cuts at: 6545 6545
Size: 7816
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7816
AlwNI CAG_nnn'CTG
Cuts at: 2363 2735 5559 7131 2363
Size: 372 2824 1572 3048
Fragments arranged by size:
3048 2824 1572 372
ApaLI G'TGCA_C
Cuts at: 4137 5873 7029 4137
Size: 1736 1156 4924
Fragments arranged by size:
4924 1736 1156
ApoI r'AATT_y
Cuts at: 1493 2661 2900 3031 3298 3872 4161 5125 5351
Size: 1168 239 131 267 574 289 964 226
Cuts at: 5351 5503 7612 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1168 964 574 289 267 239
226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 2865 6370 160
Size: 872 1833 3505 1606
Fragments arranged by size:
3505 1833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
AvaI C'yCGr_G
Cuts at: 1870 1943 5141 1870
Size: 73 3198 4545
Fragments arranged by size:
4545 3198 73
BamHI G'GATC_C
Cuts at: 5146 5513 5146
Size: 367 7449
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4109 5137 6592 618
Size: 325 217 627 2322 1028 1455 1842
Fragments arranged by size:
2322 1842 1455 1028 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2171 4569 4916 5135 1391
Size: 780 2398 347 219 4072
Fragments arranged by size:
4072 2398 780 347 219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 3257 4263 928
Size: 971 636 722 1006 4481
Fragments arranged by size:
4481 1006 971 722 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6007 6041 1903
Size: 34 4070 34 3678
Fragments arranged by size:
4070 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5708 6918 5708
Size: 1210 6606
BclI T'GATC_A
Cuts at: 1548 4026 1548
Size: 2478 5338
BfrBI ATG'CAT
Cuts at: 2120 2120
Size: 7816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6427 136
Size: 107 122 71 631 605 4755 1525
Fragments arranged by size:
4755 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4898 5024 5066 7811 4898
Size: 126 42 2745 4903
Fragments arranged by size:
4903 2745 126 42
BlpI GC'TnA_GC
Cuts at: 2298 5233 2298
Size: 2935 4881
Bme1580I G_kGCm'C
Cuts at: 1790 2203 4114 4141 5877 7033 1790
Size: 413 1911 27 1736 1156 2573
Fragments arranged by size:
2573 1911 1736 1156 413 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7816
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5053 5547 6505
Size: 416 53 83 399 24 3636 494 958
Cuts at: 6505 442
Size: 1753
Fragments arranged by size:
3636 1753 958 494 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6476 1239
Size: 5237 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2224 5941 6806 7104 2224
Size: 3717 865 298 2936
Fragments arranged by size:
3717 2936 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5085 6479 882
Size: 617 3586 1394 2219
Fragments arranged by size:
3586 2219 1394 617
BsaAI yAC'GTr
Cuts at: 493 1204 3341 5113 5607 493
Size: 711 2137 1772 494 2702
Fragments arranged by size:
2702 2137 1772 711 494
BsaBI GATnn'nnATC
Cuts at: 4224 4978 7810 4224
Size: 754 2832 4230
Fragments arranged by size:
4230 2832 754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5623 6005 6640 275
Size: 53 83 186 936 4090 382 635 1451
Fragments arranged by size:
4090 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3158 6249 6647 6921 7068 7479 3158
Size: 3091 398 274 147 411 3495
Fragments arranged by size:
3495 3091 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7591
Fragments arranged by size:
7591 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 2390 2712 1186
Size: 1028 176 322 6290
Fragments arranged by size:
6290 1028 322 176
BseYI C'CCAG_C
Cuts at: 4820 7019 7515 4820
Size: 2199 496 5121
Fragments arranged by size:
5121 2199 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5266 6027 7055 1540
Size: 275 186 3265 761 1028 2301
Fragments arranged by size:
3265 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3622 4141 4569 4916 5877 5962 7033 3622
Size: 519 428 347 961 85 1071 4405
Fragments arranged by size:
4405 1071 961 519 428 347 85
BsmI GAATG_Cn'
Cuts at: 2270 5327 5420 2270
Size: 3057 93 4666
Fragments arranged by size:
4666 3057 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2203 3622 4114 4141 4569 4916
Size: 399 381 32 1419 492 27 428 347
Cuts at: 4916 5135 5877 5962 7033 1391
Size: 219 742 85 1071 2174
Fragments arranged by size:
2174 1419 1071 742 492 428 399 381
347 219 85 32 27
BspEI T'CCGG_A
Cuts at: 3158 7479 3158
Size: 4321 3495
BspHI T'CATG_A
Cuts at: 5705 7435 5705
Size: 1730 6086
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5171 844
Size: 4327 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3190 5703 1394
Size: 429 1367 2513 3507
Fragments arranged by size:
3507 2513 1367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3705 6311 6485 65
Size: 1183 2457 2606 174 1396
Fragments arranged by size:
2606 2457 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6460 6647 1700
Size: 110 4650 187 2869
Fragments arranged by size:
4650 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3828 96
Size: 3732 4084
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7816
BssSI C'ACGA_G
Cuts at: 5876 6888 5876
Size: 1012 6804
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7816
BstBI TT'CG_AA
Cuts at: 2007 6620 2007
Size: 4613 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7816
BstXI CCAn_nnnn'nTGG
Cuts at: 2903 3486 4302 2903
Size: 583 816 6417
Fragments arranged by size:
6417 816 583
BstYI r'GATC_y
Cuts at: 1972 2045 2096 2830 3521 4898 5024 5054 5066
Size: 73 51 734 691 1377 126 30 12
Cuts at: 5066 5146 5513 5898 5915 7367 7453 7465 7811
Size: 80 367 385 17 1452 86 12 346
Cuts at: 7811 1972
Size: 1977
Fragments arranged by size:
1977 1452 1377 734 691 385 367 346
126 86 80 73 51 30 17 12
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6307
Fragments arranged by size:
6307 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7077
Fragments arranged by size:
7077 683 56
BtsI GCAGTG_nn'
Cuts at: 4846 5332 6126 6146 4846
Size: 486 794 20 6516
Fragments arranged by size:
6516 794 486 20
ClaI AT'CG_AT
Cuts at: 1554 4185 1554
Size: 2631 5185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7781
DraI TTT'AAA
Cuts at: 3027 4285 5120 3027
Size: 1258 835 5723
Fragments arranged by size:
5723 1258 835
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6823 809
Size: 507 5507 1802
Fragments arranged by size:
5507 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5263 6152 7781
Size: 54 1425 50 275 18 3433 889 1629
Cuts at: 7781 8
Size: 43
Fragments arranged by size:
3433 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5263 1537
Size: 275 3451 4090
Fragments arranged by size:
4090 3451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 2571 2645 3001 3567 3841 4119 4793
Size: 900 318 74 356 566 274 278 674
Cuts at: 4793 5746 1353
Size: 953 3423
Fragments arranged by size:
3423 953 900 674 566 356 318 278
274 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2058 3108 3505 6403 6777
Size: 1064 138 453 16 1050 397 2898 374
Cuts at: 6777 6923 387
Size: 146 1280
Fragments arranged by size:
2898 1280 1064 1050 453 397 374 146
138 16
EcoICRI GAG'CTC
Cuts at: 4567 4914 4567
Size: 347 7469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 3744 3753 3822 3967 4275 4476
Size: 393 453 1659 9 69 145 308 201
Cuts at: 4476 5879 6476 7263 7531 1239
Size: 1403 597 787 268 1524
Fragments arranged by size:
1659 1524 1403 787 597 453 393 308
268 201 145 69 9
EcoRI G'AATT_C
Cuts at: 4161 5125 5503 4161
Size: 964 378 6474
Fragments arranged by size:
6474 964 378
FspI TGC'GCA
Cuts at: 3177 6322 3177
Size: 3145 4671
HaeII r_GCGC'y
Cuts at: 1678 6963 1678
Size: 5285 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 3749 3781 6457
Size: 32 135 32 903 32 1242 32 2676
Cuts at: 6457 6489 6531 6563 1373
Size: 32 42 32 2626
Fragments arranged by size:
2676 2626 1242 903 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5160 5406 669
Size: 988 281 3222 246 3079
Fragments arranged by size:
3222 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3449 3588 748
Size: 2701 139 4976
Fragments arranged by size:
4976 2701 139
HpaI GTT'AAC
Cuts at: 5406 5406
Size: 7816
KpnI G_GTAC'C
Cuts at: 5141 5141
Size: 7816
MfeI C'AATT_G
Cuts at: 5415 5415
Size: 7816
MluI A'CGCG_T
Cuts at: 7692 7692
Size: 7816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2132 3503 4805 6930 7114 1305
Size: 827 1371 1302 2125 184 2007
Fragments arranged by size:
2125 2007 1371 1302 827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5994
Fragments arranged by size:
5994 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 4300 5774 6133 6292 518
Size: 648 949 2185 1474 359 159 2042
Fragments arranged by size:
2185 2042 1474 949 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2360 5232 5909 7057 7302 1606
Size: 754 2872 677 1148 245 2120
Fragments arranged by size:
2872 2120 1148 754 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6307
Fragments arranged by size:
6307 957 552
NdeI CA'TA_TG
Cuts at: 387 2112 387
Size: 1725 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7816
NotI GC'GGCC_GC
Cuts at: 5263 5263
Size: 7816
NsiI A_TGCA'T
Cuts at: 2122 2122
Size: 7816
NspI r_CATG'y
Cuts at: 1132 1229 5174 6719 1132
Size: 97 3945 1545 2229
Fragments arranged by size:
3945 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7816
PciI A'CATG_T
Cuts at: 6715 6715
Size: 7816
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7816
PmeI GTTT'AAAC
Cuts at: 5120 5120
Size: 7816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 5885 5917 7398 7430 4493
Size: 32 1360 32 1481 32 4879
Fragments arranged by size:
4879 1481 1360 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7816
PsiI TTA'TAA
Cuts at: 5386 5386
Size: 7816
PstI C_TGCA'G
Cuts at: 830 3468 5168 830
Size: 2638 1700 3478
Fragments arranged by size:
3478 2638 1700
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7816
PvuII CAG'CTG
Cuts at: 2360 2360
Size: 7816
SacI G_AGCT'C
Cuts at: 4569 4916 4569
Size: 347 7469
SalI G'TCGA_C
Cuts at: 1936 5158 1936
Size: 3222 4594
SapI GCTCTTCn'nnn_
Cuts at: 3567 4119 3567
Size: 552 7264
SbfI CC_TGCA'GG
Cuts at: 5168 5168
Size: 7816
ScaI AGT'ACT
Cuts at: 4520 6064 7776 4520
Size: 1544 1712 4560
Fragments arranged by size:
4560 1712 1544
SfcI C'TryA_G
Cuts at: 826 1045 3439 3464 4654 5164 6299 6980 7171
Size: 219 2394 25 1190 510 1135 681 191
Cuts at: 7171 826
Size: 1471
Fragments arranged by size:
2394 1471 1190 1135 681 510 219 191
25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7816
SmaI CCC'GGG
Cuts at: 1872 5143 1872
Size: 3271 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2239 3623 5920 6821 7083 820
Size: 197 926 296 1384 2297 901 262 1553
Fragments arranged by size:
2297 1553 1384 926 901 296 262 197
SnaBI TAC'GTA
Cuts at: 493 5113 5607 493
Size: 4620 494 2702
Fragments arranged by size:
4620 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7816
SphI G_CATG'C
Cuts at: 1132 5174 1132
Size: 4042 3774
SspI AAT'ATT
Cuts at: 5 52 4042 4063 5740 5
Size: 47 3990 21 1677 2081
Fragments arranged by size:
3990 2081 1677 47 21
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7816
StyI C'CwwG_G
Cuts at: 513 1065 2022 2854 3674 5255 513
Size: 552 957 832 820 1581 3074
Fragments arranged by size:
3074 1581 957 832 820 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5843 5860 6013 6198 6643 325
Size: 1255 4263 17 153 185 445 1498
Fragments arranged by size:
4263 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2291 3828 4470
Size: 274 80 33 51 1443 314 1537 642
Cuts at: 4470 4518 4775 4811 6062 7774 96
Size: 48 257 36 1251 1712 138
Fragments arranged by size:
1712 1537 1443 1251 642 314 274 257
138 80 51 48 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2182 2214 4799 4831 7776 7808 2182
Size: 32 2585 32 2945 32 2190
Fragments arranged by size:
2945 2585 2190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3256 4501 1316
Size: 1940 1245 4631
Fragments arranged by size:
4631 1940 1245
XbaI T'CTAG_A
Cuts at: 5021 5152 5021
Size: 131 7685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3274 3808 3274
Size: 534 7282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7816
XmaI C'CCGG_G
Cuts at: 1870 5141 1870
Size: 3271 4545
XmnI GAAnn'nnTTC
Cuts at: 3501 5945 3501
Size: 2444 5372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5624 276
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI