Restriction map for
pFN21ASDA0326
(Circular) (Six-base) MAPSORT of: pFN21ASDA0326.seq Check: 9984 from: 1 to: 7336
pFN21ASDA0326 7336 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2567 3135 2567
Size: 568 6768
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5146 278
Size: 53 83 186 936 3610 2468
Fragments arranged by size:
3610 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4679 1937
Size: 2742 4594
Acc65I G'GTAC_C
Cuts at: 4657 4657
Size: 7336
AclI AA'CG_TT
Cuts at: 1563 5463 5836 1563
Size: 3900 373 3063
Fragments arranged by size:
3900 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2482 2650 3238 3490 3955 5399 6783 7051
Size: 850 168 588 252 465 1444 1384 268
Cuts at: 7051 1632
Size: 1917
Fragments arranged by size:
1917 1444 1384 850 588 465 268 252
168
AfeI AGC'GCT
Cuts at: 2915 2915
Size: 7336
AflII C'TTAA_G
Cuts at: 820 1017 3944 4147 820
Size: 197 2927 203 4009
Fragments arranged by size:
4009 2927 203 197
AflIII A'CryG_T
Cuts at: 1203 6235 7212 1203
Size: 5032 977 1327
Fragments arranged by size:
5032 1327 977
AgeI A'CCGG_T
Cuts at: 3380 6167 3380
Size: 2787 4549
AhdI GACnn_n'nnGTC
Cuts at: 6065 6065
Size: 7336
AleI CACnn'nnGTG
Cuts at: 1166 2094 3381 4617 1166
Size: 928 1287 1236 3885
Fragments arranged by size:
3885 1287 1236 928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3791 1184
Size: 2607 4729
AlwNI CAG_nnn'CTG
Cuts at: 5079 6651 5079
Size: 1572 5764
ApaI G_GGCC'C
Cuts at: 2051 3661 4137 2051
Size: 1610 476 5250
Fragments arranged by size:
5250 1610 476
ApaLI G'TGCA_C
Cuts at: 2728 5393 6549 2728
Size: 2665 1156 3515
Fragments arranged by size:
3515 2665 1156
ApoI r'AATT_y
Cuts at: 1493 3892 4645 4871 5023 7132 1493
Size: 2399 753 226 152 2109 1697
Fragments arranged by size:
2399 2109 1697 753 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5890 160
Size: 872 4858 1606
Fragments arranged by size:
4858 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7336
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2900 3488 3683 3902 4415 4556
Size: 73 87 870 588 195 219 513 141
Cuts at: 4556 4661 1870
Size: 105 4545
Fragments arranged by size:
4545 870 588 513 219 195 141 105
87 73
AvrII C'CTAG_G
Cuts at: 4117 4117
Size: 7336
BamHI G'GATC_C
Cuts at: 3789 4666 5033 3789
Size: 877 367 6092
Fragments arranged by size:
6092 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2523 3195 4377 4657
Size: 325 217 627 309 427 672 1182 280
Cuts at: 4657 6112 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1182 672 627 427 325 309
280 217
BanII G_rGCy'C
Cuts at: 1391 2031 2051 2078 2126 2421 2594 2678 3014
Size: 640 20 27 48 295 173 84 336
Cuts at: 3014 3350 3518 3661 3684 4137 4247 4277 4307
Size: 336 168 143 23 453 110 30 30
Cuts at: 4307 4655 1391
Size: 348 4072
Fragments arranged by size:
4072 640 453 348 336 336 295 173
168 143 110 84 48 30 30 27
23 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3647 928
Size: 971 1748 4617
Fragments arranged by size:
4617 1748 971
BbvCI CC'TCA_GC
Cuts at: 4196 4196
Size: 7336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3067 3101 5527 5561 1903
Size: 34 1130 34 2426 34 3678
Fragments arranged by size:
3678 2426 1130 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5228 6438 5228
Size: 1210 6126
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7336
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2354 5947 136
Size: 107 122 71 631 605 682 3593 1525
Fragments arranged by size:
3593 1525 682 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7331 7331
Size: 7336
BlpI GC'TnA_GC
Cuts at: 4753 4753
Size: 7336
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2648 2732 2900 3152 3236 3488 3661
Size: 261 597 84 168 252 84 252 173
Cuts at: 3661 4137 4556 5397 6553 1790
Size: 476 419 841 1156 2573
Fragments arranged by size:
2573 1156 841 597 476 419 261 252
252 173 168 84 84
BmgBI CAC'GTC
Cuts at: 1318 3535 1318
Size: 2217 5119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2270 2393 3146
Size: 416 53 83 399 24 853 123 753
Cuts at: 3146 5067 6025 442
Size: 1921 958 1753
Fragments arranged by size:
1921 1753 958 853 753 416 399 123
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 7336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2692 3629 4040 4310 4413 5996 1239
Size: 992 461 937 411 270 103 1583 2579
Fragments arranged by size:
2579 1583 992 937 461 411 270 103
Bpu10I CC'TnA_GC
Cuts at: 3176 4196 3176
Size: 1020 6316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 5461 6326 6624 2924
Size: 2537 865 298 3636
Fragments arranged by size:
3636 2537 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2157 4466 5999 882
Size: 617 658 2309 1533 2219
Fragments arranged by size:
2309 2219 1533 658 617
BsaAI yAC'GTr
Cuts at: 493 1204 4633 5127 493
Size: 711 3429 494 2702
Fragments arranged by size:
3429 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3794 7330 3794
Size: 3536 3800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5143 5525 6160 275
Size: 53 83 186 936 3610 382 635 1451
Fragments arranged by size:
3610 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2065 3380 5769 6167 6441 6588 6999 2065
Size: 1315 2389 398 274 147 411 2402
Fragments arranged by size:
2402 2389 1315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 3998 4028 1178
Size: 30 165 30 1143 30 1422 30 4486
Fragments arranged by size:
4486 1422 1143 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2162 2927 3846 4043 1186
Size: 946 30 765 919 197 4479
Fragments arranged by size:
4479 946 919 765 197 30
BseYI C'CCAG_C
Cuts at: 2305 2420 3024 3646 3676 4136 4264 6539 7035
Size: 115 604 622 30 460 128 2275 496
Cuts at: 7035 2305
Size: 2606
Fragments arranged by size:
2606 2275 622 604 496 460 128 115
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 2624 2708 2965 4199 1163
Size: 573 723 165 84 257 1234 4300
Fragments arranged by size:
4300 1234 723 573 257 165 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4360 4786 5547 6575 1540
Size: 275 186 2359 426 761 1028 2301
Fragments arranged by size:
2359 2301 1028 761 426 275 186
BsiHKAI G_wGCw'C
Cuts at: 2594 2678 2732 2762 3014 3350 3518 3572 4053
Size: 84 54 30 252 336 168 54 481
Cuts at: 4053 4247 4277 5397 5482 6553 2594
Size: 194 30 1120 85 1071 3377
Fragments arranged by size:
3377 1120 1071 481 336 252 194 168
85 84 54 54 30 30
BsmI GAATG_Cn'
Cuts at: 4181 4847 4940 4181
Size: 666 93 6577
Fragments arranged by size:
6577 666 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2051 2078 2126 2421 2594 2648
Size: 399 241 20 27 48 295 173 54
Cuts at: 2648 2678 2732 2762 2900 3014 3152 3236 3350
Size: 30 54 30 138 114 138 84 114
Cuts at: 3350 3488 3518 3572 3661 3684 4053 4137 4247
Size: 138 30 54 89 23 369 84 110
Cuts at: 4247 4277 4307 4556 4655 5397 5482 6553 1391
Size: 30 30 249 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 295 249 241
173 138 138 138 114 114 110 99
89 85 84 84 54 54 54 48
30 30 30 30 30 27 23 20
BspEI T'CCGG_A
Cuts at: 2065 6999 2065
Size: 4934 2402
BspHI T'CATG_A
Cuts at: 3342 5225 6955 3342
Size: 1883 1730 3723
Fragments arranged by size:
3723 1883 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2567 3135 4691 844
Size: 1723 568 1556 3489
Fragments arranged by size:
3489 1723 1556 568
BsrBI CCG'CTC
Cuts at: 1394 1823 2548 3220 3304 3923 5223 1394
Size: 429 725 672 84 619 1300 3507
Fragments arranged by size:
3507 1300 725 672 619 429 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 5831 6005 65
Size: 1183 4583 174 1396
Fragments arranged by size:
4583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2624 2792 3380 3464 4568 5980 6167
Size: 110 814 168 588 84 1104 1412 187
Cuts at: 6167 1700
Size: 2869
Fragments arranged by size:
2869 1412 1104 814 588 187 168 110
84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7336
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7336
BssSI C'ACGA_G
Cuts at: 3602 3737 5396 6408 3602
Size: 135 1659 1012 4530
Fragments arranged by size:
4530 1659 1012 135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2484 2602 2736 2988 794
Size: 1690 118 134 252 5142
Fragments arranged by size:
5142 1690 252 134 118
BstBI TT'CG_AA
Cuts at: 2007 6140 2007
Size: 4133 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7336
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2337 2278
Size: 59 7277
BstYI r'GATC_y
Cuts at: 1972 2053 2216 2289 2952 3789 4666 5033 5418
Size: 81 163 73 663 837 877 367 385
Cuts at: 5418 5435 6887 6973 6985 7331 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 877 837 663 385 367 346
163 86 81 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3295 4072 4501 513
Size: 552 957 1273 777 429 3348
Fragments arranged by size:
3348 1273 957 777 552 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3817 487
Size: 683 56 2591 4006
Fragments arranged by size:
4006 2591 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 4018 4852 5646 5666 2372
Size: 1646 834 794 20 4042
Fragments arranged by size:
4042 1646 834 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7301
DraI TTT'AAA
Cuts at: 4165 4640 4165
Size: 475 6861
DraIII CAC_nnn'GTG
Cuts at: 2602 2736 2988 3622 2602
Size: 134 252 634 6316
Fragments arranged by size:
6316 634 252 134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2234 6343 809
Size: 507 918 4109 1802
Fragments arranged by size:
4109 1802 918 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 3088 4783
Size: 54 1425 50 275 18 1090 168 1695
Cuts at: 4783 5672 7301 8
Size: 889 1629 43
Fragments arranged by size:
1695 1629 1425 1090 889 275 168 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4783 1537
Size: 275 2971 4090
Fragments arranged by size:
4090 2971 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2990 4235 4247 4265 4282 5266 1353
Size: 756 881 1245 12 18 17 984 3423
Fragments arranged by size:
3423 1245 984 881 756 18 17 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2474 3350 5923 6297 6443 387
Size: 1064 138 885 876 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 885 876 374 146 138
EcoICRI GAG'CTC
Cuts at: 2592 2676 3012 3348 3516 4245 4275 2592
Size: 84 336 336 168 729 30 5653
Fragments arranged by size:
5653 729 336 336 168 84 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2482 2650 2692 3238 3490 3629
Size: 393 599 251 168 42 546 252 139
Cuts at: 3629 3955 4040 4310 4413 5399 5996 6783 7051
Size: 326 85 270 103 986 597 787 268
Cuts at: 7051 1239
Size: 1524
Fragments arranged by size:
1524 986 787 599 597 546 393 326
270 268 252 251 168 139 103 85
42
EcoNI CCTnn'n_nnAGG
Cuts at: 3131 3885 3131
Size: 754 6582
EcoO109I rG'GnC_Cy
Cuts at: 2047 2227 3136 3657 3688 3928 4133 4344 4420
Size: 180 909 521 31 240 205 211 76
Cuts at: 4420 2047
Size: 4963
Fragments arranged by size:
4963 909 521 240 211 205 180 76
31
EcoRI G'AATT_C
Cuts at: 3892 4645 5023 3892
Size: 753 378 6205
Fragments arranged by size:
6205 753 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3897 3897
Size: 7336
FspI TGC'GCA
Cuts at: 5842 5842
Size: 7336
HaeII r_GCGC'y
Cuts at: 1678 2376 2917 3183 6483 1678
Size: 698 541 266 3300 2531
Fragments arranged by size:
3300 2531 698 541 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5977 6009 6051 6083 1373
Size: 32 135 32 4405 32 42 32 2626
Fragments arranged by size:
4405 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4680 4926 669
Size: 988 281 2742 246 3079
Fragments arranged by size:
3079 2742 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4186 748
Size: 3438 3898
HpaI GTT'AAC
Cuts at: 4926 4926
Size: 7336
KpnI G_GTAC'C
Cuts at: 4661 4661
Size: 7336
MfeI C'AATT_G
Cuts at: 4935 4935
Size: 7336
MluI A'CGCG_T
Cuts at: 7212 7212
Size: 7336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 3291 3429 3543 3597 6450 6634 1305
Size: 1398 588 138 114 54 2853 184 2007
Fragments arranged by size:
2853 2007 1398 588 184 138 114 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5514
Fragments arranged by size:
5514 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 3381 3545 3773 4617 5294 5653
Size: 648 928 1287 164 228 844 677 359
Cuts at: 5653 5812 518
Size: 159 2042
Fragments arranged by size:
2042 1287 928 844 677 648 359 228
164 159
MspA1I CmG'CkG
Cuts at: 1606 2361 2670 2979 3118 3297 4329 4752 5429
Size: 755 309 309 139 179 1032 423 677
Cuts at: 5429 6577 6822 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1032 755 677 423 309 309
245 179 139
NaeI GCC'GGC
Cuts at: 4570 4570
Size: 7336
NcoI C'CATG_G
Cuts at: 513 1065 2022 4501 513
Size: 552 957 2479 3348
Fragments arranged by size:
3348 2479 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7336
NgoMIV G'CCGG_C
Cuts at: 4568 4568
Size: 7336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7336
NotI GC'GGCC_GC
Cuts at: 4783 4783
Size: 7336
NspI r_CATG'y
Cuts at: 1132 1229 4694 6239 1132
Size: 97 3465 1545 2229
Fragments arranged by size:
3465 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3661 4309 1348
Size: 2313 648 4375
Fragments arranged by size:
4375 2313 648
PciI A'CATG_T
Cuts at: 6235 6235
Size: 7336
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3607 1108
Size: 2499 4837
PfoI T'CCnGG_A
Cuts at: 3591 4035 4287 3591
Size: 444 252 6640
Fragments arranged by size:
6640 444 252
PmeI GTTT'AAAC
Cuts at: 4640 4640
Size: 7336
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3545 3577 5405 5437 6918 6950 3125
Size: 32 388 32 1828 32 1481 32 3511
Fragments arranged by size:
3511 1828 1481 388 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 3136 3688 4420 2227
Size: 909 552 732 5143
Fragments arranged by size:
5143 909 732 552
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7336
PsiI TTA'TAA
Cuts at: 4906 4906
Size: 7336
PspOMI G'GGCC_C
Cuts at: 2047 3657 4133 2047
Size: 1610 476 5250
Fragments arranged by size:
5250 1610 476
PstI C_TGCA'G
Cuts at: 830 2061 4688 830
Size: 1231 2627 3478
Fragments arranged by size:
3478 2627 1231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7336
PvuII CAG'CTG
Cuts at: 2361 2670 2361
Size: 309 7027
SacI G_AGCT'C
Cuts at: 2594 2678 3014 3350 3518 4247 4277 2594
Size: 84 336 336 168 729 30 5653
Fragments arranged by size:
5653 729 336 336 168 84 30
SacII CC_GC'GG
Cuts at: 3298 3298
Size: 7336
SalI G'TCGA_C
Cuts at: 1936 4678 1936
Size: 2742 4594
SanDI GG'GwC_CC
Cuts at: 3688 3688
Size: 7336
SapI GCTCTTCn'nnn_
Cuts at: 2990 4235 4265 4282 2990
Size: 1245 30 17 6044
Fragments arranged by size:
6044 1245 30 17
SbfI CC_TGCA'GG
Cuts at: 4688 4688
Size: 7336
ScaI AGT'ACT
Cuts at: 5584 7296 5584
Size: 1712 5624
SexAI A'CCwGG_T
Cuts at: 2598 2682 2598
Size: 84 7252
SfcI C'TryA_G
Cuts at: 826 1045 2057 4684 5819 6500 6691 826
Size: 219 1012 2627 1135 681 191 1471
Fragments arranged by size:
2627 1471 1135 1012 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2354 2354
Size: 7336
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7336
SgrAI Cr'CCGG_yG
Cuts at: 1700 2624 2792 3380 3464 1700
Size: 924 168 588 84 5572
Fragments arranged by size:
5572 924 588 168 84
SmaI CCC'GGG
Cuts at: 1872 3904 4663 1872
Size: 2032 759 4545
Fragments arranged by size:
4545 2032 759
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2939 3944 4147 5440 6341 6603
Size: 197 926 996 1005 203 1293 901 262
Cuts at: 6603 820
Size: 1553
Fragments arranged by size:
1553 1293 1005 996 926 901 262 203
197
SnaBI TAC'GTA
Cuts at: 493 4633 5127 493
Size: 4140 494 2702
Fragments arranged by size:
4140 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7336
SphI G_CATG'C
Cuts at: 1132 4694 1132
Size: 3562 3774
SspI AAT'ATT
Cuts at: 5 52 5260 5
Size: 47 5208 2081
Fragments arranged by size:
5208 2081 47
StuI AGG'CCT
Cuts at: 1517 2348 3587 1517
Size: 831 1239 5266
Fragments arranged by size:
5266 1239 831
StyI C'CwwG_G
Cuts at: 513 1065 2022 2187 2277 2343 3872 4117 4501
Size: 552 957 165 90 66 1529 245 384
Cuts at: 4501 4775 513
Size: 274 3074
Fragments arranged by size:
3074 1529 957 552 384 274 245 165
90 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 2885 4085 4409 4629 5363 5380
Size: 1255 1188 117 1200 324 220 734 17
Cuts at: 5380 5533 5718 6163 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 1188 734 445 324 220
185 153 117 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5582 7294 96
Size: 274 80 33 51 1443 3605 1712 138
Fragments arranged by size:
3605 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 7296 7328 2768
Size: 32 4496 32 2776
Fragments arranged by size:
4496 2776 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7336
XbaI T'CTAG_A
Cuts at: 4672 4672
Size: 7336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4528 4528
Size: 7336
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7336
XmaI C'CCGG_G
Cuts at: 1870 3902 4661 1870
Size: 2032 759 4545
Fragments arranged by size:
4545 2032 759
XmnI GAAnn'nnTTC
Cuts at: 2747 2915 2999 3560 3587 5465 2747
Size: 168 84 561 27 1878 4618
Fragments arranged by size:
4618 1878 561 168 84 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5144 276
Size: 53 83 186 936 3610 2468
Fragments arranged by size:
3610 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SfoI SrfI SwaI