Restriction map for
pFN21ASDA0319
(Circular) (Six-base) MAPSORT of: pFN21ASDA0319.seq Check: 7161 from: 1 to: 7945
pFN21ASDA0319 7945 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3374 5755 278
Size: 53 83 186 936 1838 2381 2468
Fragments arranged by size:
2468 2381 1838 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 3848 4250 5288 1937
Size: 250 1661 402 1038 4594
Fragments arranged by size:
4594 1661 1038 402 250
Acc65I G'GTAC_C
Cuts at: 5266 5266
Size: 7945
AclI AA'CG_TT
Cuts at: 1563 6072 6445 1563
Size: 4509 373 3063
Fragments arranged by size:
4509 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2736 3104 3453 3839 4692 4787 5128 5200
Size: 1104 368 349 386 853 95 341 72
Cuts at: 5200 6008 7392 7660 1632
Size: 808 1384 268 1917
Fragments arranged by size:
1917 1384 1104 853 808 386 368 349
341 268 95 72
AfeI AGC'GCT
Cuts at: 4808 4808
Size: 7945
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7748
AflIII A'CryG_T
Cuts at: 1203 3742 4201 6844 7821 1203
Size: 2539 459 2643 977 1327
Fragments arranged by size:
2643 2539 1327 977 459
AgeI A'CCGG_T
Cuts at: 6776 6776
Size: 7945
AhdI GACnn_n'nnGTC
Cuts at: 3230 6674 3230
Size: 3444 4501
AleI CACnn'nnGTG
Cuts at: 1166 2039 2327 4532 1166
Size: 873 288 2205 4579
Fragments arranged by size:
4579 2205 873 288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7945
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2808 2840 2378
Size: 32 398 32 7483
Fragments arranged by size:
7483 398 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2362 3902 4123 4310 4430 5688 7260 2362
Size: 1540 221 187 120 1258 1572 3047
Fragments arranged by size:
3047 1572 1540 1258 221 187 120
ApaI G_GGCC'C
Cuts at: 2306 3450 4600 2306
Size: 1144 1150 5651
Fragments arranged by size:
5651 1150 1144
ApaLI G'TGCA_C
Cuts at: 6002 7158 6002
Size: 1156 6789
ApoI r'AATT_y
Cuts at: 1493 3139 5254 5480 5632 7741 1493
Size: 1646 2115 226 152 2109 1697
Fragments arranged by size:
2115 2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6499 160
Size: 872 5467 1606
Fragments arranged by size:
5467 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7945
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 2911 4185 5270 1870
Size: 73 458 510 1274 1085 4545
Fragments arranged by size:
4545 1274 1085 510 458 73
AvrII C'CTAG_G
Cuts at: 4457 4457
Size: 7945
BamHI G'GATC_C
Cuts at: 4284 5275 5642 4284
Size: 991 367 6587
Fragments arranged by size:
6587 991 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2635 5266 6721 618
Size: 325 217 627 239 609 2631 1455 1842
Fragments arranged by size:
2631 1842 1455 627 609 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2292 2306 2396 2550 2667 2868 3357 3450
Size: 901 14 90 154 117 201 489 93
Cuts at: 3450 4186 4600 5264 1391
Size: 736 414 664 4072
Fragments arranged by size:
4072 901 736 664 489 414 201 154
117 93 90 14
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 7945
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2837 3226 3470 3982 928
Size: 971 938 389 244 512 4891
Fragments arranged by size:
4891 971 938 512 389 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6136 6170 1903
Size: 34 4199 34 3678
Fragments arranged by size:
4199 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5116 5837 7047 5116
Size: 721 1210 6014
Fragments arranged by size:
6014 1210 721
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6556 136
Size: 107 122 71 631 605 4884 1525
Fragments arranged by size:
4884 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7940 7940
Size: 7945
BlpI GC'TnA_GC
Cuts at: 2867 5362 2867
Size: 2495 5450
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2306 3450 4600 6006 7162 1790
Size: 296 220 1144 1150 1406 1156 2573
Fragments arranged by size:
2573 1406 1156 1150 1144 296 220
BmgBI CAC'GTC
Cuts at: 1318 4009 4134 1318
Size: 2691 125 5129
Fragments arranged by size:
5129 2691 125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2526 2584 2888
Size: 416 53 83 399 24 1109 58 304
Cuts at: 2888 3929 4009 4014 5676 6634 442
Size: 1041 80 5 1662 958 1753
Fragments arranged by size:
1753 1662 1109 1041 958 416 399 304
83 80 58 53 24 5
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7945
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 5107 2887
Size: 2220 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2774 2882 3060 3879 4058 4332 5077
Size: 1292 243 108 178 819 179 274 745
Cuts at: 5077 6605 1239
Size: 1528 2579
Fragments arranged by size:
2579 1528 1292 819 745 274 243 179
178 108
Bpu10I CC'TnA_GC
Cuts at: 2661 2661
Size: 7945
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4759 6070 6935 7233 4759
Size: 1311 865 298 5471
Fragments arranged by size:
5471 1311 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 6608 882
Size: 617 2577 2532 2219
Fragments arranged by size:
2577 2532 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5242 5736 493
Size: 711 4038 494 2702
Fragments arranged by size:
4038 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7939 7939
Size: 7945
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 3371 3767 5752
Size: 53 83 186 936 494 1344 396 1985
Cuts at: 5752 6134 6769 275
Size: 382 635 1451
Fragments arranged by size:
1985 1451 1344 936 635 494 396 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6378 6776 7050 7197 7608 6378
Size: 398 274 147 411 6715
Fragments arranged by size:
6715 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 2989 3019 4361
Size: 30 165 30 975 30 581 30 1342
Cuts at: 4361 4391 1178
Size: 30 4732
Fragments arranged by size:
4732 1342 975 581 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 2602 1186
Size: 1303 113 6529
Fragments arranged by size:
6529 1303 113
BseYI C'CCAG_C
Cuts at: 7148 7644 7148
Size: 496 7449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 2176 2337 4044 4466 4673 1163
Size: 573 380 60 161 1707 422 207 4435
Fragments arranged by size:
4435 1707 573 422 380 207 161 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 5395 6156 7184 1540
Size: 275 186 198 3196 761 1028 2301
Fragments arranged by size:
3196 2301 1028 761 275 198 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2334 2667 2868 2901 2928 3357 4008 5088
Size: 288 333 201 33 27 429 651 1080
Cuts at: 5088 6006 6091 7162 2046
Size: 918 85 1071 2829
Fragments arranged by size:
2829 1080 1071 918 651 429 333 288
201 85 33 27
BsmI GAATG_Cn'
Cuts at: 5456 5549 5456
Size: 93 7852
BsmBI CGTCTCn'nnnn_
Cuts at: 2639 2639
Size: 7945
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2086 2292 2306 2334 2396 2550
Size: 399 256 40 206 14 28 62 154
Cuts at: 2550 2667 2868 2901 2928 3357 3450 4008 4186
Size: 117 201 33 27 429 93 558 178
Cuts at: 4186 4600 5088 5264 6006 6091 7162 1391
Size: 414 488 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 558 488 429 414 399
256 206 201 178 176 154 117 93
85 62 40 33 28 27 14
BspEI T'CCGG_A
Cuts at: 7608 7608
Size: 7945
BspHI T'CATG_A
Cuts at: 5834 7564 5834
Size: 1730 6215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2066 3324 4480 4745 5300 844
Size: 1222 1258 1156 265 555 3489
Fragments arranged by size:
3489 1258 1222 1156 555 265
BsrBI CCG'CTC
Cuts at: 1394 1823 5832 1394
Size: 429 4009 3507
Fragments arranged by size:
4009 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2051 3795 6440 6614 65
Size: 1183 803 1744 2645 174 1396
Fragments arranged by size:
2645 1744 1396 1183 803 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3908 6589 6776 1700
Size: 110 2098 2681 187 2869
Fragments arranged by size:
2869 2681 2098 187 110
BsrGI T'GTAC_A
Cuts at: 96 4377 4633 96
Size: 4281 256 3408
Fragments arranged by size:
4281 3408 256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7945
BssSI C'ACGA_G
Cuts at: 6005 7017 6005
Size: 1012 6933
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2081 4959 794
Size: 1287 2878 3780
Fragments arranged by size:
3780 2878 1287
BstBI TT'CG_AA
Cuts at: 2007 6749 2007
Size: 4742 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7945
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 4027 4234 5101 2299
Size: 1728 207 867 5143
Fragments arranged by size:
5143 1728 867 207
BstYI r'GATC_y
Cuts at: 1972 2406 4284 4606 5275 5642 6027 6044 7496
Size: 434 1878 322 669 367 385 17 1452
Cuts at: 7496 7582 7594 7940 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1878 1452 669 434 385 367 346
322 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2424 2424
Size: 7945
BtgI C'CryG_G
Cuts at: 513 1065 2022 2880 3979 4780 513
Size: 552 957 858 1099 801 3678
Fragments arranged by size:
3678 1099 957 858 801 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3987 487
Size: 683 56 2761 4445
Fragments arranged by size:
4445 2761 683 56
BtsI GCAGTG_nn'
Cuts at: 2099 3000 3688 4032 4428 4786 5461 6255 6275
Size: 901 688 344 396 358 675 794 20
Cuts at: 6275 2099
Size: 3769
Fragments arranged by size:
3769 901 794 688 675 396 358 344
20
ClaI AT'CG_AT
Cuts at: 1554 4323 1554
Size: 2769 5176
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7910
DraI TTT'AAA
Cuts at: 5249 5249
Size: 7945
DraIII CAC_nnn'GTG
Cuts at: 4342 4342
Size: 7945
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3482 4580 6952 809
Size: 507 2166 1098 2372 1802
Fragments arranged by size:
2372 2166 1802 1098 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 3906 4294
Size: 54 1425 50 275 18 366 1710 388
Cuts at: 4294 4777 5392 6281 7910 8
Size: 483 615 889 1629 43
Fragments arranged by size:
1710 1629 1425 889 615 483 388 366
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2196 5392 1537
Size: 275 384 3196 4090
Fragments arranged by size:
4090 3196 384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 3301 3453 4457 5875 1353
Size: 700 1248 152 1004 1418 3423
Fragments arranged by size:
3423 1418 1248 1004 700 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6532 6906 7052 387
Size: 1064 138 4943 374 146 1280
Fragments arranged by size:
4943 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2665 2866 3355 2665
Size: 201 489 7255
Fragments arranged by size:
7255 489 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2736 2774 2882 3060 3104 3453
Size: 393 899 205 38 108 178 44 349
Cuts at: 3453 3839 3879 4058 4332 4692 4787 5077 5128
Size: 386 40 179 274 360 95 290 51
Cuts at: 5128 5200 6008 6605 7392 7660 1239
Size: 72 808 597 787 268 1524
Fragments arranged by size:
1524 899 808 787 597 393 386 360
349 290 274 268 205 179 178 108
95 72 51 44 40 38
EcoO109I rG'GnC_Cy
Cuts at: 2303 2467 2518 2578 2601 2656 3447 3722 4016
Size: 164 51 60 23 55 791 275 294
Cuts at: 4016 4083 4413 2303
Size: 67 330 5835
Fragments arranged by size:
5835 791 330 294 275 164 67 60
55 51 23
EcoRI G'AATT_C
Cuts at: 5254 5632 5254
Size: 378 7567
EcoRV GAT'ATC
Cuts at: 2431 2431
Size: 7945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 4735 2539
Size: 2196 5749
FseI GG_CCGG'CC
Cuts at: 3912 3912
Size: 7945
FspI TGC'GCA
Cuts at: 6451 6451
Size: 7945
HaeII r_GCGC'y
Cuts at: 1678 2030 3107 4810 7092 1678
Size: 352 1077 1703 2282 2531
Fragments arranged by size:
2531 2282 1703 1077 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 3216 3248 4305
Size: 32 135 32 893 32 719 32 1057
Cuts at: 4305 4337 5155 5187 6586 6618 6660 6692 1373
Size: 32 818 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1399 1057 893 818 719 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3313 3475 3531 5289 5535 669
Size: 988 281 1375 162 56 1758 246 3079
Fragments arranged by size:
3079 1758 1375 988 281 246 162 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7945
HpaI GTT'AAC
Cuts at: 5535 5535
Size: 7945
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 7945
KpnI G_GTAC'C
Cuts at: 5270 5270
Size: 7945
MfeI C'AATT_G
Cuts at: 2070 3212 5544 2070
Size: 1142 2332 4471
Fragments arranged by size:
4471 2332 1142
MluI A'CGCG_T
Cuts at: 7821 7821
Size: 7945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2599 2745 3202 7059 7243 1305
Size: 1294 146 457 3857 184 2007
Fragments arranged by size:
3857 2007 1294 457 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4296 4779 10
Size: 54 1425 343 2464 483 3176
Fragments arranged by size:
3176 2464 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2162 2327 4532 5903 6262 6421
Size: 648 873 123 165 2205 1371 359 159
Cuts at: 6421 518
Size: 2042
Fragments arranged by size:
2205 2042 1371 873 648 359 165 159
123
MspA1I CmG'CkG
Cuts at: 1606 2220 2250 2359 2642 3817 4310 4507 4519
Size: 614 30 109 283 1175 493 197 12
Cuts at: 4519 4543 5361 6038 7186 7431 1606
Size: 24 818 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 818 677 614 493 283
245 197 109 30 24 12
NaeI GCC'GGC
Cuts at: 3910 3910
Size: 7945
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 7945
NcoI C'CATG_G
Cuts at: 513 1065 2022 4780 513
Size: 552 957 2758 3678
Fragments arranged by size:
3678 2758 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7945
NgoMIV G'CCGG_C
Cuts at: 3908 3908
Size: 7945
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7945
NotI GC'GGCC_GC
Cuts at: 5392 5392
Size: 7945
NspI r_CATG'y
Cuts at: 1132 1229 3746 4205 4696 5303 6848 1132
Size: 97 2517 459 491 607 1545 2229
Fragments arranged by size:
2517 2229 1545 607 491 459 97
PasI CC'CwG_GG
Cuts at: 1348 3718 1348
Size: 2370 5575
PciI A'CATG_T
Cuts at: 3742 4201 6844 3742
Size: 459 2643 4843
Fragments arranged by size:
4843 2643 459
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4310 4342 1108
Size: 3202 32 4711
Fragments arranged by size:
4711 3202 32
PfoI T'CCnGG_A
Cuts at: 2790 4287 5013 2790
Size: 1497 726 5722
Fragments arranged by size:
5722 1497 726
PmeI GTTT'AAAC
Cuts at: 5249 5249
Size: 7945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2378 2410 6014 6046 7527 7559 2378
Size: 32 3604 32 1481 32 2764
Fragments arranged by size:
3604 2764 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2656 3722 4083 2518
Size: 138 1066 361 6380
Fragments arranged by size:
6380 1066 361 138
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7945
PsiI TTA'TAA
Cuts at: 3056 5515 3056
Size: 2459 5486
PspOMI G'GGCC_C
Cuts at: 2302 3446 4596 2302
Size: 1144 1150 5651
Fragments arranged by size:
5651 1150 1144
PstI C_TGCA'G
Cuts at: 830 3574 4353 4491 5297 830
Size: 2744 779 138 806 3478
Fragments arranged by size:
3478 2744 806 779 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7945
PvuII CAG'CTG
Cuts at: 2220 2359 3817 4310 4507 4543 2220
Size: 139 1458 493 197 36 5622
Fragments arranged by size:
5622 1458 493 197 139 36
SacI G_AGCT'C
Cuts at: 2667 2868 3357 2667
Size: 201 489 7255
Fragments arranged by size:
7255 489 201
SalI G'TCGA_C
Cuts at: 1936 5287 1936
Size: 3351 4594
SbfI CC_TGCA'GG
Cuts at: 4491 5297 4491
Size: 806 7139
ScaI AGT'ACT
Cuts at: 6193 7905 6193
Size: 1712 6233
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 7945
SfcI C'TryA_G
Cuts at: 826 1045 3111 3324 3517 3570 3855 4349 4487
Size: 219 2066 213 193 53 285 494 138
Cuts at: 4487 5293 6428 7109 7300 826
Size: 806 1135 681 191 1471
Fragments arranged by size:
2066 1471 1135 806 681 494 285 219
213 193 191 138 53
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 7945
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7945
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7945
SmaI CCC'GGG
Cuts at: 1872 2913 4187 5272 1872
Size: 1041 1274 1085 4545
Fragments arranged by size:
4545 1274 1085 1041
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4738 6049 6950 7212 820
Size: 197 926 2795 1311 901 262 1553
Fragments arranged by size:
2795 1553 1311 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5242 5736 493
Size: 4749 494 2702
Fragments arranged by size:
4749 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7945
SphI G_CATG'C
Cuts at: 1132 5303 1132
Size: 4171 3774
SrfI GCCC'GGGC
Cuts at: 4187 4187
Size: 7945
SspI AAT'ATT
Cuts at: 5 52 4042 5869 5
Size: 47 3990 1827 2081
Fragments arranged by size:
3990 2081 1827 47
StuI AGG'CCT
Cuts at: 1517 2350 3089 1517
Size: 833 739 6373
Fragments arranged by size:
6373 833 739
StyI C'CwwG_G
Cuts at: 513 1065 2022 3166 4457 4780 5384 513
Size: 552 957 1144 1291 323 604 3074
Fragments arranged by size:
3074 1291 1144 957 604 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5972 5989 6142 6327 6772 325
Size: 1255 4392 17 153 185 445 1498
Fragments arranged by size:
4392 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2565 2918 3398
Size: 274 80 33 51 1443 588 353 480
Cuts at: 3398 3760 4377 4633 5004 6191 7903 96
Size: 362 617 256 371 1187 1712 138
Fragments arranged by size:
1712 1443 1187 617 588 480 371 362
353 274 256 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7905 7937 7905
Size: 32 7913
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7945
XbaI T'CTAG_A
Cuts at: 5281 5281
Size: 7945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 4144 2224
Size: 1920 6025
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7945
XmaI C'CCGG_G
Cuts at: 1870 2911 4185 5270 1870
Size: 1041 1274 1085 4545
Fragments arranged by size:
4545 1274 1085 1041
XmnI GAAnn'nnTTC
Cuts at: 6074 6074
Size: 7945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3372 5753 276
Size: 53 83 186 936 1838 2381 2468
Fragments arranged by size:
2468 2381 1838 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbvCI BfrBI BsiWI BstZ17I EcoNI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SfiI SwaI