Restriction map for
pFN21ASDA0317
(Circular) (Six-base) MAPSORT of: pFN21ASDA0317.seq Check: 9062 from: 1 to: 7198
pFN21ASDA0317 7198 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2970 2970
Size: 7198
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5008 278
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4541 1937
Size: 2604 4594
Acc65I G'GTAC_C
Cuts at: 4519 4519
Size: 7198
AclI AA'CG_TT
Cuts at: 1563 5325 5698 1563
Size: 3762 373 3063
Fragments arranged by size:
3762 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 2877 3149 3434 4262 5261 6645 6913
Size: 728 517 272 285 828 999 1384 268
Cuts at: 6913 1632
Size: 1917
Fragments arranged by size:
1917 1384 999 828 728 517 285 272
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7001
AflIII A'CryG_T
Cuts at: 1203 2104 4388 6097 7074 1203
Size: 901 2284 1709 977 1327
Fragments arranged by size:
2284 1709 1327 977 901
AgeI A'CCGG_T
Cuts at: 6029 6029
Size: 7198
AhdI GACnn_n'nnGTC
Cuts at: 5927 5927
Size: 7198
AleI CACnn'nnGTG
Cuts at: 1166 3224 1166
Size: 2058 5140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 2496 2528 2304
Size: 32 160 32 6974
Fragments arranged by size:
6974 160 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2054 4941 6513 2054
Size: 2887 1572 2739
Fragments arranged by size:
2887 2739 1572
ApaLI G'TGCA_C
Cuts at: 4436 5255 6411 4436
Size: 819 1156 5223
Fragments arranged by size:
5223 1156 819
ApoI r'AATT_y
Cuts at: 1493 2352 2490 2831 3179 3463 3833 4003 4507
Size: 859 138 341 348 284 370 170 504
Cuts at: 4507 4733 4885 6994 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 859 504 370 348 341 284
226 170 152 138
AseI AT'TA_AT
Cuts at: 160 1032 5752 160
Size: 872 4720 1606
Fragments arranged by size:
4720 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 2775 4523 1870
Size: 73 652 180 1748 4545
Fragments arranged by size:
4545 1748 652 180 73
BamHI G'GATC_C
Cuts at: 2188 4528 4895 2188
Size: 2340 367 4491
Fragments arranged by size:
4491 2340 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2958 3144 4519 5974 618
Size: 325 217 627 1171 186 1375 1455 1842
Fragments arranged by size:
1842 1455 1375 1171 627 325 217 186
BanII G_rGCy'C
Cuts at: 1391 2385 2676 3261 3757 3964 4517 1391
Size: 994 291 585 496 207 553 4072
Fragments arranged by size:
4072 994 585 553 496 291 207
BbeI G_GCGC'C
Cuts at: 3148 3148
Size: 7198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3033 928
Size: 971 1134 5093
Fragments arranged by size:
5093 1134 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5389 5423 1903
Size: 34 3452 34 3678
Fragments arranged by size:
3678 3452 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5090 6300 5090
Size: 1210 5988
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7198
BfrBI ATG'CAT
Cuts at: 3802 3802
Size: 7198
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5809 136
Size: 107 122 71 631 605 4137 1525
Fragments arranged by size:
4137 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7193 7193
Size: 7198
BlpI GC'TnA_GC
Cuts at: 3260 4615 3260
Size: 1355 5843
Bme1580I G_kGCm'C
Cuts at: 1790 4440 5259 6415 1790
Size: 2650 819 1156 2573
Fragments arranged by size:
2650 2573 1156 819
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3538 4929 5887
Size: 416 53 83 399 24 2121 1391 958
Cuts at: 5887 442
Size: 1753
Fragments arranged by size:
2121 1753 1391 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2345 1056
Size: 1289 5909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 3954 4164 4332 5858 1239
Size: 866 1849 210 168 1526 2579
Fragments arranged by size:
2579 1849 1526 866 210 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2656 2667 4142 5323 6188 6486 2656
Size: 11 1475 1181 865 298 3368
Fragments arranged by size:
3368 1475 1181 865 298 11
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3326 5861 882
Size: 617 1827 2535 2219
Fragments arranged by size:
2535 2219 1827 617
BsaAI yAC'GTr
Cuts at: 493 1204 2105 2170 4495 4989 493
Size: 711 901 65 2325 494 2702
Fragments arranged by size:
2702 2325 901 711 494 65
BsaBI GATnn'nnATC
Cuts at: 7192 7192
Size: 7198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3145 5005 5387 6022
Size: 53 83 186 936 1612 1860 382 635
Cuts at: 6022 275
Size: 1451
Fragments arranged by size:
1860 1612 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2151 5631 6029 6303 6450 6861 2151
Size: 3480 398 274 147 411 2488
Fragments arranged by size:
3480 2488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6973
Fragments arranged by size:
6973 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3744 4058 4274 4285 1186
Size: 2558 314 216 11 4099
Fragments arranged by size:
4099 2558 314 216 11
BseYI C'CCAG_C
Cuts at: 2426 2988 6401 6897 2426
Size: 562 3413 496 2727
Fragments arranged by size:
3413 2727 562 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2280 4365 1163
Size: 573 544 2085 3996
Fragments arranged by size:
3996 2085 573 544
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4648 5409 6437 1540
Size: 275 186 2647 761 1028 2301
Fragments arranged by size:
2647 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2649 2676 3261 3757 3964 4440 5259 5344 6415
Size: 27 585 496 207 476 819 85 1071
Cuts at: 6415 2649
Size: 3432
Fragments arranged by size:
3432 1071 819 585 496 476 207 85
27
BsmI GAATG_Cn'
Cuts at: 2063 2770 3240 4709 4802 2063
Size: 707 470 1469 93 4459
Fragments arranged by size:
4459 1469 707 470 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 7198
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2649 2676 3261 3757 3964 4440
Size: 399 595 264 27 585 496 207 476
Cuts at: 4440 4517 5259 5344 6415 1391
Size: 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 585 496 476 399
264 207 85 77 27
BspEI T'CCGG_A
Cuts at: 6861 6861
Size: 7198
BspHI T'CATG_A
Cuts at: 2668 3948 4218 5087 6817 2668
Size: 1280 270 869 1730 3049
Fragments arranged by size:
3049 1730 1280 869 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2970 3367 4553 844
Size: 2126 397 1186 3489
Fragments arranged by size:
3489 2126 1186 397
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 3656 3768 4355 5085 1394
Size: 429 1480 353 112 587 730 3507
Fragments arranged by size:
3507 1480 730 587 429 353 112
BsrDI GCAATG_nn'
Cuts at: 65 1248 2887 5693 5867 65
Size: 1183 1639 2806 174 1396
Fragments arranged by size:
2806 1639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2994 5842 6029 1700
Size: 110 1184 2848 187 2869
Fragments arranged by size:
2869 2848 1184 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7198
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7198
BssSI C'ACGA_G
Cuts at: 3695 5258 6270 3695
Size: 1563 1012 4623
Fragments arranged by size:
4623 1563 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4444 794
Size: 3650 3548
BstBI TT'CG_AA
Cuts at: 2007 6002 2007
Size: 3995 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7198
BstYI r'GATC_y
Cuts at: 1972 2188 4528 4895 5280 5297 6749 6835 6847
Size: 216 2340 367 385 17 1452 86 12
Cuts at: 6847 7193 1972
Size: 346 1977
Fragments arranged by size:
2340 1977 1452 385 367 346 216 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2954 513
Size: 552 1889 4757
Fragments arranged by size:
4757 1889 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6459
Fragments arranged by size:
6459 683 56
BtsI GCAGTG_nn'
Cuts at: 2352 3003 4714 5508 5528 2352
Size: 651 1711 794 20 4022
Fragments arranged by size:
4022 1711 794 651 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2992 3027 529
Size: 35 2428 35 4700
Fragments arranged by size:
4700 2428 35 35
DraI TTT'AAA
Cuts at: 4002 4502 4002
Size: 500 6698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2843 6205 809
Size: 507 1527 3362 1802
Fragments arranged by size:
3362 1802 1527 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2996 4026 4455
Size: 54 1425 50 275 18 1166 1030 429
Cuts at: 4455 4645 5534 7163 8
Size: 190 889 1629 43
Fragments arranged by size:
1629 1425 1166 1030 889 429 275 190
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4645 1537
Size: 275 2833 4090
Fragments arranged by size:
4090 2833 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 2989 3602 3897 4425 5128 1353
Size: 1332 304 613 295 528 703 3423
Fragments arranged by size:
3423 1332 703 613 528 304 295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5785 6159 6305 387
Size: 1064 138 4196 374 146 1280
Fragments arranged by size:
4196 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2674 3259 3755 3962 2674
Size: 585 496 207 5910
Fragments arranged by size:
5910 585 496 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2360 2877 3149 3434 3954 4164
Size: 393 473 255 517 272 285 520 210
Cuts at: 4164 4262 4332 5261 5858 6645 6913 1239
Size: 98 70 929 597 787 268 1524
Fragments arranged by size:
1524 929 787 597 520 517 473 393
285 272 268 255 210 98 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2852 2852
Size: 7198
EcoO109I rG'GnC_Cy
Cuts at: 2224 3536 2224
Size: 1312 5886
EcoRI G'AATT_C
Cuts at: 3833 4507 4885 3833
Size: 674 378 6146
Fragments arranged by size:
6146 674 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 3370 2104
Size: 1266 5932
FspI TGC'GCA
Cuts at: 2420 5704 2420
Size: 3284 3914
HaeII r_GCGC'y
Cuts at: 1678 2143 2995 3148 6345 1678
Size: 465 852 153 3197 2531
Fragments arranged by size:
3197 2531 852 465 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 2310 2342 3014
Size: 32 135 32 608 32 98 32 672
Cuts at: 3014 3020 3046 3052 4200 4232 5839 5871 5913
Size: 6 26 6 1148 32 1607 32 42
Cuts at: 5913 5945 1373
Size: 32 2626
Fragments arranged by size:
2626 1607 1148 672 608 135 98 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3013 4542 4788 669
Size: 988 281 1075 1529 246 3079
Fragments arranged by size:
3079 1529 1075 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 748
Size: 1702 5496
HpaI GTT'AAC
Cuts at: 4788 4788
Size: 7198
KasI G'GCGC_C
Cuts at: 3144 3144
Size: 7198
KpnI G_GTAC'C
Cuts at: 4523 4523
Size: 7198
MfeI C'AATT_G
Cuts at: 3926 4797 3926
Size: 871 6327
MluI A'CGCG_T
Cuts at: 7074 7074
Size: 7198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 2754 3417 4382 6312 6496 1305
Size: 1110 339 663 965 1930 184 2007
Fragments arranged by size:
2007 1930 1110 965 663 339 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4028 4457 10
Size: 54 1425 343 2196 429 2751
Fragments arranged by size:
2751 2196 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2975 3224 4154 4387 5156 5515 5674
Size: 648 1809 249 930 233 769 359 159
Cuts at: 5674 518
Size: 2042
Fragments arranged by size:
2042 1809 930 769 648 359 249 233
159
MspA1I CmG'CkG
Cuts at: 1606 3264 4402 4453 4614 5291 6439 6684 1606
Size: 1658 1138 51 161 677 1148 245 2120
Fragments arranged by size:
2120 1658 1148 1138 677 245 161 51
NaeI GCC'GGC
Cuts at: 2996 2996
Size: 7198
NarI GG'CG_CC
Cuts at: 3145 3145
Size: 7198
NcoI C'CATG_G
Cuts at: 513 1065 2954 513
Size: 552 1889 4757
Fragments arranged by size:
4757 1889 552
NdeI CA'TA_TG
Cuts at: 387 3384 4417 387
Size: 2997 1033 3168
Fragments arranged by size:
3168 2997 1033
NgoMIV G'CCGG_C
Cuts at: 2994 2994
Size: 7198
NheI G'CTAG_C
Cuts at: 1052 2341 1052
Size: 1289 5909
NotI GC'GGCC_GC
Cuts at: 4645 4645
Size: 7198
NsiI A_TGCA'T
Cuts at: 3804 3804
Size: 7198
NspI r_CATG'y
Cuts at: 1132 1229 3431 4392 4489 4556 6101 1132
Size: 97 2202 961 97 67 1545 2229
Fragments arranged by size:
2229 2202 1545 961 97 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7198
PciI A'CATG_T
Cuts at: 4388 6097 4388
Size: 1709 5489
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 1108
Size: 1140 6058
PfoI T'CCnGG_A
Cuts at: 3169 3507 3169
Size: 338 6860
PmeI GTTT'AAAC
Cuts at: 4502 4502
Size: 7198
PmlI CAC'GTG
Cuts at: 2105 2105
Size: 7198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5267 5299 6780 6812 5267
Size: 32 1481 32 5653
Fragments arranged by size:
5653 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2224
Size: 7198
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7198
PsiI TTA'TAA
Cuts at: 2928 4768 2928
Size: 1840 5358
PstI C_TGCA'G
Cuts at: 830 4452 4550 830
Size: 3622 98 3478
Fragments arranged by size:
3622 3478 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7198
PvuII CAG'CTG
Cuts at: 3264 4402 4453 3264
Size: 1138 51 6009
Fragments arranged by size:
6009 1138 51
SacI G_AGCT'C
Cuts at: 2676 3261 3757 3964 2676
Size: 585 496 207 5910
Fragments arranged by size:
5910 585 496 207
SalI G'TCGA_C
Cuts at: 1936 4540 1936
Size: 2604 4594
SapI GCTCTTCn'nnn_
Cuts at: 3602 3602
Size: 7198
SbfI CC_TGCA'GG
Cuts at: 4550 4550
Size: 7198
ScaI AGT'ACT
Cuts at: 2698 3812 5446 7158 2698
Size: 1114 1634 1712 2738
Fragments arranged by size:
2738 1712 1634 1114
SfcI C'TryA_G
Cuts at: 826 1045 2941 4448 4546 5681 6362 6553 826
Size: 219 1896 1507 98 1135 681 191 1471
Fragments arranged by size:
1896 1507 1471 1135 681 219 191 98
SfoI GGC'GCC
Cuts at: 3146 3146
Size: 7198
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7198
SmaI CCC'GGG
Cuts at: 1872 4525 1872
Size: 2653 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2635 2682 2775 4121 5302 6203
Size: 197 926 692 47 93 1346 1181 901
Cuts at: 6203 6465 820
Size: 262 1553
Fragments arranged by size:
1553 1346 1181 926 901 692 262 197
93 47
SnaBI TAC'GTA
Cuts at: 493 4495 4989 493
Size: 4002 494 2702
Fragments arranged by size:
4002 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7198
SphI G_CATG'C
Cuts at: 1132 4489 4556 1132
Size: 3357 67 3774
Fragments arranged by size:
3774 3357 67
SspI AAT'ATT
Cuts at: 5 52 5122 5
Size: 47 5070 2081
Fragments arranged by size:
5070 2081 47
StuI AGG'CCT
Cuts at: 1517 4068 1517
Size: 2551 4647
StyI C'CwwG_G
Cuts at: 513 1065 2954 3190 4637 513
Size: 552 1889 236 1447 3074
Fragments arranged by size:
3074 1889 1447 552 236
SwaI ATTT'AAAT
Cuts at: 4002 4002
Size: 7198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2983 5225 5242 5395 5580 6025 325
Size: 1255 1403 2242 17 153 185 445 1498
Fragments arranged by size:
2242 1498 1403 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2696 3081 3810
Size: 274 80 33 51 1443 719 385 729
Cuts at: 3810 5444 7156 96
Size: 1634 1712 138
Fragments arranged by size:
1712 1634 1443 729 719 385 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2034 2066 2451 2483 3681 3713 7158 7190 2034
Size: 32 385 32 1198 32 3445 32 2042
Fragments arranged by size:
3445 2042 1198 385 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3203 1316
Size: 1887 5311
XbaI T'CTAG_A
Cuts at: 4534 4534
Size: 7198
XhoI C'TCGA_G
Cuts at: 1943 2775 1943
Size: 832 6366
XmaI C'CCGG_G
Cuts at: 1870 4523 1870
Size: 2653 4545
XmnI GAAnn'nnTTC
Cuts at: 2373 3119 3356 5327 2373
Size: 746 237 1971 4244
Fragments arranged by size:
4244 1971 746 237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5006 276
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI XcmI