Restriction map for
pFN21ASDA0307
(Circular) (Six-base) MAPSORT of: pFN21ASDA0307.seq Check: 5797 from: 1 to: 6847
pFN21ASDA0307 6847 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3142 3142
Size: 6847
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4657 278
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4190 1937
Size: 2253 4594
Acc65I G'GTAC_C
Cuts at: 4168 4168
Size: 6847
AclI AA'CG_TT
Cuts at: 1563 4974 5347 1563
Size: 3411 373 3063
Fragments arranged by size:
3411 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2013 2423 2648 2901 3296 3583 3591 4053
Size: 381 410 225 253 395 287 8 462
Cuts at: 4053 4910 6294 6562 1632
Size: 857 1384 268 1917
Fragments arranged by size:
1917 1384 857 462 410 395 381 287
268 253 225 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6650
AflIII A'CryG_T
Cuts at: 1203 3666 5746 6723 1203
Size: 2463 2080 977 1327
Fragments arranged by size:
2463 2080 1327 977
AgeI A'CCGG_T
Cuts at: 5678 5678
Size: 6847
AhdI GACnn_n'nnGTC
Cuts at: 3717 5576 3717
Size: 1859 4988
AleI CACnn'nnGTG
Cuts at: 1166 3035 1166
Size: 1869 4978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6847
AlwNI CAG_nnn'CTG
Cuts at: 2401 3530 4590 6162 2401
Size: 1129 1060 1572 3086
Fragments arranged by size:
3086 1572 1129 1060
ApaLI G'TGCA_C
Cuts at: 2587 3338 4904 6060 2587
Size: 751 1566 1156 3374
Fragments arranged by size:
3374 1566 1156 751
ApoI r'AATT_y
Cuts at: 1493 2174 3104 4156 4382 4534 6643 1493
Size: 681 930 1052 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 930 681 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5401 160
Size: 872 4369 1606
Fragments arranged by size:
4369 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
AvaI C'yCGr_G
Cuts at: 1870 1943 4172 1870
Size: 73 2229 4545
Fragments arranged by size:
4545 2229 73
BamHI G'GATC_C
Cuts at: 3040 4177 4544 3040
Size: 1137 367 5343
Fragments arranged by size:
5343 1137 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4168 5623 618
Size: 325 217 627 1199 1182 1455 1842
Fragments arranged by size:
1842 1455 1199 1182 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2247 2687 3524 4166 1391
Size: 856 440 837 642 4072
Fragments arranged by size:
4072 856 837 642 440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2636 2892 4107 928
Size: 971 737 256 1215 3668
Fragments arranged by size:
3668 1215 971 737 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4088 4122 5038 5072 1903
Size: 34 2151 34 916 34 3678
Fragments arranged by size:
3678 2151 916 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2834 4739 5949 2834
Size: 1905 1210 3732
Fragments arranged by size:
3732 1905 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6847
BfrBI ATG'CAT
Cuts at: 2964 2964
Size: 6847
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 5458 136
Size: 107 122 71 631 605 397 3389 1525
Fragments arranged by size:
3389 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 3549 6842 3549
Size: 3293 3554
BlpI GC'TnA_GC
Cuts at: 3523 4264 3523
Size: 741 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2591 2991 3342 4908 6064 1790
Size: 801 400 351 1566 1156 2573
Fragments arranged by size:
2573 1566 1156 801 400 351
BmgBI CAC'GTC
Cuts at: 1318 2563 1318
Size: 1245 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2163 2506 2643
Size: 416 53 83 399 24 746 343 137
Cuts at: 2643 4017 4578 5536 442
Size: 1374 561 958 1753
Fragments arranged by size:
1753 1374 958 746 561 416 399 343
137 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6847
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4024 4024
Size: 6847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2924 3645 3823 3862 3882 5507 1239
Size: 1685 721 178 39 20 1625 2579
Fragments arranged by size:
2579 1685 1625 721 178 39 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4972 5837 6135 4972
Size: 865 298 5684
Fragments arranged by size:
5684 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5510 882
Size: 617 4011 2219
Fragments arranged by size:
4011 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4144 4638 493
Size: 711 2940 494 2702
Fragments arranged by size:
2940 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3224 3358 6841 3224
Size: 134 3483 3230
Fragments arranged by size:
3483 3230 134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4654 5036 5671 275
Size: 53 83 186 936 3121 382 635 1451
Fragments arranged by size:
3121 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2078 2132 5280 5678 5952 6099 6510 2078
Size: 54 3148 398 274 147 411 2415
Fragments arranged by size:
3148 2415 411 398 274 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2938 2968 3206 3236 1178
Size: 30 165 30 1535 30 238 30 4789
Fragments arranged by size:
4789 1535 238 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3844 1186
Size: 2658 4189
BseYI C'CCAG_C
Cuts at: 3543 3844 6050 6546 3543
Size: 301 2206 496 3844
Fragments arranged by size:
3844 2206 496 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3271 1163
Size: 573 1535 4739
Fragments arranged by size:
4739 1535 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4297 5058 6086 1540
Size: 275 186 2296 761 1028 2301
Fragments arranged by size:
2301 2296 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2534 2591 3342 3524 4908 4993 6064 2247
Size: 287 57 751 182 1384 85 1071 3030
Fragments arranged by size:
3030 1384 1071 751 287 182 85 57
BsmI GAATG_Cn'
Cuts at: 3874 4358 4451 3874
Size: 484 93 6270
Fragments arranged by size:
6270 484 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2323 2323
Size: 6847
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2534 2591 2687 2991 3342 3524
Size: 399 457 287 57 96 304 351 182
Cuts at: 3524 4166 4908 4993 6064 1391
Size: 642 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 642 457 399 351 304
287 182 96 85 57
BspEI T'CCGG_A
Cuts at: 2132 6510 2132
Size: 4378 2469
BspHI T'CATG_A
Cuts at: 3409 4736 6466 3409
Size: 1327 1730 3790
Fragments arranged by size:
3790 1730 1327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3142 4202 844
Size: 2298 1060 3489
Fragments arranged by size:
3489 2298 1060
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 4734 1394
Size: 429 2173 738 3507
Fragments arranged by size:
3507 2173 738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5342 5516 65
Size: 1183 4094 174 1396
Fragments arranged by size:
4094 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3401 5491 5678 1700
Size: 110 1591 2090 187 2869
Fragments arranged by size:
2869 2090 1591 187 110
BsrGI T'GTAC_A
Cuts at: 96 2834 96
Size: 2738 4109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6847
BssSI C'ACGA_G
Cuts at: 2921 4907 5919 2921
Size: 1986 1012 3849
Fragments arranged by size:
3849 1986 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6847
BstBI TT'CG_AA
Cuts at: 2007 5651 2007
Size: 3644 3203
BstEII G'GTnAC_C
Cuts at: 1184 2944 1184
Size: 1760 5087
BstXI CCAn_nnnn'nTGG
Cuts at: 3350 3983 4082 3350
Size: 633 99 6115
Fragments arranged by size:
6115 633 99
BstYI r'GATC_y
Cuts at: 1972 2042 2616 3040 3079 3463 3549 4066 4177
Size: 70 574 424 39 384 86 517 111
Cuts at: 4177 4544 4929 4946 6398 6484 6496 6842 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 574 517 424 385 384 367
346 111 86 86 70 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3117 3117
Size: 6847
BtgI C'CryG_G
Cuts at: 513 1065 2022 2067 2113 3426 3654 3982 513
Size: 552 957 45 46 1313 228 328 3378
Fragments arranged by size:
3378 1313 957 552 328 228 46 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6108
Fragments arranged by size:
6108 683 56
BtsI GCAGTG_nn'
Cuts at: 3971 4363 5157 5177 3971
Size: 392 794 20 5641
Fragments arranged by size:
5641 794 392 20
ClaI AT'CG_AT
Cuts at: 1554 3193 1554
Size: 1639 5208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6812
DraI TTT'AAA
Cuts at: 4151 4151
Size: 6847
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5854 809
Size: 507 4538 1802
Fragments arranged by size:
4538 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2313 2855
Size: 54 1425 50 275 18 243 240 542
Cuts at: 2855 3985 4294 5183 6812 8
Size: 1130 309 889 1629 43
Fragments arranged by size:
1629 1425 1130 889 542 309 275 243
240 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4294 1537
Size: 275 2482 4090
Fragments arranged by size:
4090 2482 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 3652 3826 4777 1353
Size: 2257 42 174 951 3423
Fragments arranged by size:
3423 2257 951 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3452 3714 5434 5808 5954 387
Size: 1064 138 1863 262 1720 374 146 1280
Fragments arranged by size:
1863 1720 1280 1064 374 262 146 138
EcoICRI GAG'CTC
Cuts at: 2245 3522 2245
Size: 1277 5570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2423 2648 2901 2924 3296 3583
Size: 393 381 410 225 253 23 372 287
Cuts at: 3583 3591 3645 3823 3862 3882 4053 4910 5507
Size: 8 54 178 39 20 171 857 597
Cuts at: 5507 6294 6562 1239
Size: 787 268 1524
Fragments arranged by size:
1524 857 787 597 410 393 381 372
287 268 253 225 178 171 54 39
23 20 8
EcoO109I rG'GnC_Cy
Cuts at: 2165 3379 2165
Size: 1214 5633
EcoRI G'AATT_C
Cuts at: 3104 4156 4534 3104
Size: 1052 378 5417
Fragments arranged by size:
5417 1052 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 6847
FspI TGC'GCA
Cuts at: 5353 5353
Size: 6847
HaeII r_GCGC'y
Cuts at: 1678 5994 1678
Size: 4316 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2938 2970 2987 3019 3341
Size: 32 135 32 1366 32 17 32 322
Cuts at: 3341 3373 3626 3658 3703 3729 3735 3761 5488
Size: 32 253 32 45 26 6 26 1727
Cuts at: 5488 5520 5562 5594 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1727 1366 322 253 135 45 42
32 32 32 32 32 32 32 32
26 26 17 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4191 4437 669
Size: 988 281 2253 246 3079
Fragments arranged by size:
3079 2253 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6847
HpaI GTT'AAC
Cuts at: 4437 4437
Size: 6847
KpnI G_GTAC'C
Cuts at: 4172 4172
Size: 6847
MfeI C'AATT_G
Cuts at: 2928 4446 2928
Size: 1518 5329
MluI A'CGCG_T
Cuts at: 6723 6723
Size: 6847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 4094 5961 6145 1305
Size: 969 1820 1867 184 2007
Fragments arranged by size:
2007 1867 1820 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2857 3987 10
Size: 54 1425 343 1025 1130 2870
Fragments arranged by size:
2870 1425 1130 1025 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2556 2672 2969 3035 3135 4805 5164
Size: 648 1390 116 297 66 100 1670 359
Cuts at: 5164 5323 518
Size: 159 2042
Fragments arranged by size:
2042 1670 1390 648 359 297 159 116
100 66
MspA1I CmG'CkG
Cuts at: 1606 2426 4263 4940 6088 6333 1606
Size: 820 1837 677 1148 245 2120
Fragments arranged by size:
2120 1837 1148 820 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2067 3982 513
Size: 552 957 45 1915 3378
Fragments arranged by size:
3378 1915 957 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6847
NotI GC'GGCC_GC
Cuts at: 4294 4294
Size: 6847
NsiI A_TGCA'T
Cuts at: 2966 2966
Size: 6847
NspI r_CATG'y
Cuts at: 1132 1229 2268 3670 4051 4123 4205 5750 1132
Size: 97 1039 1402 381 72 82 1545 2229
Fragments arranged by size:
2229 1545 1402 1039 381 97 82 72
PasI CC'CwG_GG
Cuts at: 1348 4075 1348
Size: 2727 4120
PciI A'CATG_T
Cuts at: 3666 5746 3666
Size: 2080 4767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3154 1108
Size: 2046 4801
PfoI T'CCnGG_A
Cuts at: 3587 3733 4041 3587
Size: 146 308 6393
Fragments arranged by size:
6393 308 146
PmeI GTTT'AAAC
Cuts at: 4151 4151
Size: 6847
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 6429 6461 4916
Size: 32 1481 32 5302
Fragments arranged by size:
5302 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3379 3379
Size: 6847
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6847
PsiI TTA'TAA
Cuts at: 4417 4417
Size: 6847
PstI C_TGCA'G
Cuts at: 830 2708 3535 4199 830
Size: 1878 827 664 3478
Fragments arranged by size:
3478 1878 827 664
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6847
PvuII CAG'CTG
Cuts at: 2426 2426
Size: 6847
SacI G_AGCT'C
Cuts at: 2247 3524 2247
Size: 1277 5570
SalI G'TCGA_C
Cuts at: 1936 4189 1936
Size: 2253 4594
SapI GCTCTTCn'nnn_
Cuts at: 3610 3610
Size: 6847
SbfI CC_TGCA'GG
Cuts at: 4199 4199
Size: 6847
ScaI AGT'ACT
Cuts at: 5095 6807 5095
Size: 1712 5135
SfcI C'TryA_G
Cuts at: 826 1045 2704 3531 3826 4195 5330 6011 6202
Size: 219 1659 827 295 369 1135 681 191
Cuts at: 6202 826
Size: 1471
Fragments arranged by size:
1659 1471 1135 827 681 369 295 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6847
SmaI CCC'GGG
Cuts at: 1872 4174 1872
Size: 2302 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4951 5852 6114 820
Size: 197 926 3008 901 262 1553
Fragments arranged by size:
3008 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4144 4638 493
Size: 3651 494 2702
Fragments arranged by size:
3651 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6847
SphI G_CATG'C
Cuts at: 1132 4205 1132
Size: 3073 3774
SspI AAT'ATT
Cuts at: 5 52 2915 4771 5
Size: 47 2863 1856 2081
Fragments arranged by size:
2863 2081 1856 47
StuI AGG'CCT
Cuts at: 1517 2852 3596 1517
Size: 1335 744 4768
Fragments arranged by size:
4768 1335 744
StyI C'CwwG_G
Cuts at: 513 1065 2022 2067 2788 3559 3937 3982 4286
Size: 552 957 45 721 771 378 45 304
Cuts at: 4286 513
Size: 3074
Fragments arranged by size:
3074 957 771 721 552 378 304 45
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3172 4874 4891 5044 5229 5674 325
Size: 1255 1592 1702 17 153 185 445 1498
Fragments arranged by size:
1702 1592 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 2834 3276
Size: 274 80 33 51 1443 255 602 442
Cuts at: 3276 5093 6805 96
Size: 1817 1712 138
Fragments arranged by size:
1817 1712 1443 602 442 274 255 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2701 2733 6807 6839 2315
Size: 32 354 32 4074 32 2323
Fragments arranged by size:
4074 2323 354 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2255 2486 3769 1316
Size: 939 231 1283 4394
Fragments arranged by size:
4394 1283 939 231
XbaI T'CTAG_A
Cuts at: 4183 4183
Size: 6847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 2308
Size: 6847
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6847
XmaI C'CCGG_G
Cuts at: 1870 4172 1870
Size: 2302 4545
XmnI GAAnn'nnTTC
Cuts at: 2774 3596 4976 2774
Size: 822 1380 4645
Fragments arranged by size:
4645 1380 822
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4655 276
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI