Restriction map for
pFN21ASDA0293
(Circular) (Six-base) MAPSORT of: pFN21ASDA0293.seq Check: 8363 from: 1 to: 9187
pFN21ASDA0293 9187 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3173 3173
Size: 9187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2477 6997 278
Size: 53 83 186 936 941 4520 2468
Fragments arranged by size:
4520 2468 941 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4710 6530 1937
Size: 2773 1820 4594
Fragments arranged by size:
4594 2773 1820
Acc65I G'GTAC_C
Cuts at: 6508 6508
Size: 9187
AclI AA'CG_TT
Cuts at: 1563 7314 7687 1563
Size: 5751 373 3063
Fragments arranged by size:
5751 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2984 3216 4670 5333 5837 7250 8634 8902
Size: 1352 232 1454 663 504 1413 1384 268
Cuts at: 8902 1632
Size: 1917
Fragments arranged by size:
1917 1454 1413 1384 1352 663 504 268
232
AfeI AGC'GCT
Cuts at: 2661 2661
Size: 9187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8990
AflIII A'CryG_T
Cuts at: 1203 4695 5166 8086 9063 1203
Size: 3492 471 2920 977 1327
Fragments arranged by size:
3492 2920 1327 977 471
AgeI A'CCGG_T
Cuts at: 8018 8018
Size: 9187
AhdI GACnn_n'nnGTC
Cuts at: 3385 4825 5278 5455 7916 3385
Size: 1440 453 177 2461 4656
Fragments arranged by size:
4656 2461 1440 453 177
AleI CACnn'nnGTG
Cuts at: 1166 6363 1166
Size: 5197 3990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4953 6381 1184
Size: 3769 1428 3990
Fragments arranged by size:
3990 3769 1428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6461 6493 6461
Size: 32 9155
AlwNI CAG_nnn'CTG
Cuts at: 3001 3165 3409 3581 3721 3868 4153 5003 5227
Size: 164 244 172 140 147 285 850 224
Cuts at: 5227 5490 5509 6930 8502 3001
Size: 263 19 1421 1572 3686
Fragments arranged by size:
3686 1572 1421 850 285 263 244 224
172 164 147 140 19
ApaI G_GGCC'C
Cuts at: 2490 2874 2912 3419 3660 4246 4283 5864 6066
Size: 384 38 507 241 586 37 1581 202
Cuts at: 6066 2490
Size: 5611
Fragments arranged by size:
5611 1581 586 507 384 241 202 38
37
ApaLI G'TGCA_C
Cuts at: 7244 8400 7244
Size: 1156 8031
ApoI r'AATT_y
Cuts at: 1493 2174 3242 6496 6722 6874 8983 1493
Size: 681 1068 3254 226 152 2109 1697
Fragments arranged by size:
3254 2109 1697 1068 681 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2309 7741 160
Size: 872 1277 5432 1606
Fragments arranged by size:
5432 1606 1277 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9187
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 2525 2905 3398 3659 3934 4523
Size: 73 495 87 380 493 261 275 589
Cuts at: 4523 4676 5156 5535 5552 6059 6512 1870
Size: 153 480 379 17 507 453 4545
Fragments arranged by size:
4545 589 507 495 493 480 453 380
379 275 261 153 87 73 17
BamHI G'GATC_C
Cuts at: 6080 6517 6884 6080
Size: 437 367 8383
Fragments arranged by size:
8383 437 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3560 4171 4189 4291 4937
Size: 325 217 627 1773 611 18 102 646
Cuts at: 4937 5548 6299 6508 7963 618
Size: 611 751 209 1455 1842
Fragments arranged by size:
1842 1773 1455 751 646 627 611 611
325 217 209 102 18
BanII G_rGCy'C
Cuts at: 1391 2445 2490 2874 2912 2990 3419 3627 3660
Size: 1054 45 384 38 78 429 208 33
Cuts at: 3660 3782 3846 4032 4246 4283 4578 4725 4875
Size: 122 64 186 214 37 295 147 150
Cuts at: 4875 5015 5264 5622 5804 5864 6066 6506 1391
Size: 140 249 358 182 60 202 440 4072
Fragments arranged by size:
4072 1054 440 429 384 358 295 249
214 208 202 186 182 150 147 140
122 78 64 60 45 38 37 33
BbeI G_GCGC'C
Cuts at: 3564 4175 4295 6303 3564
Size: 611 120 2008 6448
Fragments arranged by size:
6448 2008 611 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 3291 3384 928
Size: 971 1305 87 93 6731
Fragments arranged by size:
6731 1305 971 93 87
BbvCI CC'TCA_GC
Cuts at: 2447 2781 3348 4584 5378 5943 6271 2447
Size: 334 567 1236 794 565 328 5363
Fragments arranged by size:
5363 1236 794 567 565 334 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7378 7412 1903
Size: 34 5441 34 3678
Fragments arranged by size:
5441 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7079 8289 7079
Size: 1210 7977
BclI T'GATC_A
Cuts at: 1548 2745 1548
Size: 1197 7990
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2485 2517 2930
Size: 107 122 71 631 605 813 32 413
Cuts at: 2930 3590 4946 7798 136
Size: 660 1356 2852 1525
Fragments arranged by size:
2852 1525 1356 813 660 631 605 413
122 107 71 32
BglII A'GATC_T
Cuts at: 9182 9182
Size: 9187
BlpI GC'TnA_GC
Cuts at: 3128 4052 4409 5306 6604 3128
Size: 924 357 897 1298 5711
Fragments arranged by size:
5711 1298 924 897 357
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2874 2912 3326 3419 3660 4194 4246
Size: 700 384 38 414 93 241 534 52
Cuts at: 4246 4283 4521 5483 5864 6066 7248 8404 1790
Size: 37 238 962 381 202 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 962 700 534 414 384
381 241 238 202 93 52 38 37
BmgBI CAC'GTC
Cuts at: 1318 2971 1318
Size: 1653 7534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2385 2630 2868
Size: 416 53 83 399 24 968 245 238
Cuts at: 2868 3091 3329 3620 4396 6357 6918 7876 442
Size: 223 238 291 776 1961 561 958 1753
Fragments arranged by size:
1961 1753 968 958 776 561 416 399
291 245 238 238 223 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3976 5029 5092 5641 3976
Size: 1053 63 549 7522
Fragments arranged by size:
7522 1053 549 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2780 2939 2966 3023 3056 3074 3142
Size: 827 714 159 27 57 33 18 68
Cuts at: 3142 3272 4133 4178 4739 5060 5062 5417 5867
Size: 130 861 45 561 321 2 355 450
Cuts at: 5867 6215 6369 6488 7847 1239
Size: 348 154 119 1359 2579
Fragments arranged by size:
2579 1359 861 827 714 561 450 355
348 321 159 154 130 119 68 57
45 33 27 18 2
Bpu10I CC'TnA_GC
Cuts at: 2447 2781 3348 3603 3621 3896 4584 5378 5510
Size: 334 567 255 18 275 688 794 132
Cuts at: 5510 5943 6271 2447
Size: 433 328 5363
Fragments arranged by size:
5363 794 688 567 433 334 328 275
255 132 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4562 5627 7312 8177 8475 4562
Size: 1065 1685 865 298 5274
Fragments arranged by size:
5274 1685 1065 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 7850 882
Size: 617 904 5447 2219
Fragments arranged by size:
5447 2219 904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3760 4636 5169 6484 6978 493
Size: 711 2556 876 533 1315 494 2702
Fragments arranged by size:
2702 2556 1315 876 711 533 494
BsaBI GATnn'nnATC
Cuts at: 9181 9181
Size: 9187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2474 3561 3969 4095
Size: 53 83 186 936 941 1087 408 126
Cuts at: 4095 4172 4292 4365 6300 6994 7376 8011 275
Size: 77 120 73 1935 694 382 635 1451
Fragments arranged by size:
1935 1451 1087 941 936 694 635 408
382 186 126 120 83 77 73 53
BsaWI w'CCGG_w
Cuts at: 7620 8018 8292 8439 8850 7620
Size: 398 274 147 411 7957
Fragments arranged by size:
7957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2064 2094 3995 4025 4376
Size: 30 165 30 661 30 1901 30 351
Cuts at: 4376 4406 5156 5186 6461 6491 1178
Size: 30 750 30 1275 30 3874
Fragments arranged by size:
3874 1901 1275 750 661 351 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2435 2648 2726 3020 3683 3686 4280
Size: 958 291 213 78 294 663 3 594
Cuts at: 4280 4438 4441 4444 4447 5074 5570 5612 5882
Size: 158 3 3 3 627 496 42 270
Cuts at: 5882 5948 6026 6217 6256 6259 6473 1186
Size: 66 78 191 39 3 214 3900
Fragments arranged by size:
3900 958 663 627 594 496 294 291
270 214 213 191 158 78 78 66
42 39 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2378 2790 3260 3392 3412 3419 3467 3502 3547
Size: 412 470 132 20 7 48 35 45
Cuts at: 3547 3763 4792 4988 5483 8390 8886 2378
Size: 216 1029 196 495 2907 496 2679
Fragments arranged by size:
2907 2679 1029 496 495 470 412 216
196 132 48 45 35 20 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2103 2993 4346 1163
Size: 573 367 890 1353 6004
Fragments arranged by size:
6004 1353 890 573 367
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 4486 4599 4835 6222 6637
Size: 275 186 2398 87 113 236 1387 415
Cuts at: 6637 7398 8426 1540
Size: 761 1028 2301
Fragments arranged by size:
2398 2301 1387 1028 761 415 275 236
186 113 87
BsiHKAI G_wGCw'C
Cuts at: 2445 2906 2990 4578 4725 5015 5622 7248 7333
Size: 461 84 1588 147 290 607 1626 85
Cuts at: 7333 8404 2445
Size: 1071 3228
Fragments arranged by size:
3228 1626 1588 1071 607 461 290 147
85 84
BsmI GAATG_Cn'
Cuts at: 2520 3981 6698 6791 2520
Size: 1461 2717 93 4916
Fragments arranged by size:
4916 2717 1461 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2445 2490 2874 2906 2912 2990 3326
Size: 399 655 45 384 32 6 78 336
Cuts at: 3326 3419 3627 3660 3782 3846 4032 4194 4246
Size: 93 208 33 122 64 186 162 52
Cuts at: 4246 4283 4521 4578 4725 4875 5015 5264 5483
Size: 37 238 57 147 150 140 249 219
Cuts at: 5483 5622 5804 5864 6066 6506 7248 7333 8404
Size: 139 182 60 202 440 742 85 1071
Cuts at: 8404 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 655 440 399 384 336
249 238 219 208 202 186 182 162
150 147 140 139 122 93 85 78
64 60 57 52 45 37 33 32
6
BspEI T'CCGG_A
Cuts at: 8850 8850
Size: 9187
BspHI T'CATG_A
Cuts at: 2748 6240 7076 8806 2748
Size: 3492 836 1730 3129
Fragments arranged by size:
3492 3129 1730 836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 5283 6542 844
Size: 2329 2110 1259 3489
Fragments arranged by size:
3489 2329 2110 1259
BsrBI CCG'CTC
Cuts at: 1394 1823 3040 3650 4185 4210 4297 5076 5094
Size: 429 1217 610 535 25 87 779 18
Cuts at: 5094 5472 6442 7074 1394
Size: 378 970 632 3507
Fragments arranged by size:
3507 1217 970 779 632 610 535 429
378 87 25 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 7682 7856 65
Size: 1183 6434 174 1396
Fragments arranged by size:
6434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4367 5441 7831 8018 1700
Size: 110 2557 1074 2390 187 2869
Fragments arranged by size:
2869 2557 2390 1074 187 110
BsrGI T'GTAC_A
Cuts at: 96 4692 96
Size: 4596 4591
BssHII G'CGCG_C
Cuts at: 1925 4608 1925
Size: 2683 6504
BssSI C'ACGA_G
Cuts at: 7247 8259 7247
Size: 1012 8175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3409 3760 4153 794
Size: 2615 351 393 5828
Fragments arranged by size:
5828 2615 393 351
BstBI TT'CG_AA
Cuts at: 2007 7991 2007
Size: 5984 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9187
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 3151 3268 4934 2848
Size: 303 117 1666 7101
Fragments arranged by size:
7101 1666 303 117
BstYI r'GATC_y
Cuts at: 1972 2949 3792 4773 6080 6517 6884 7269 7286
Size: 977 843 981 1307 437 367 385 17
Cuts at: 7286 8738 8824 8836 9182 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1307 981 977 843 437 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2193 3279 5865 2193
Size: 1086 2586 5515
Fragments arranged by size:
5515 2586 1086
BtgI C'CryG_G
Cuts at: 513 1065 2022 3627 4399 4498 4841 513
Size: 552 957 1605 772 99 343 4859
Fragments arranged by size:
4859 1605 957 772 552 343 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4042 487
Size: 683 56 2816 5632
Fragments arranged by size:
5632 2816 683 56
BtsI GCAGTG_nn'
Cuts at: 2990 3203 4926 5567 6333 6703 7497 7517 2990
Size: 213 1723 641 766 370 794 20 4660
Fragments arranged by size:
4660 1723 794 766 641 370 213 20
ClaI AT'CG_AT
Cuts at: 1554 2042 1554
Size: 488 8699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9152
DraI TTT'AAA
Cuts at: 6491 6491
Size: 9187
DraIII CAC_nnn'GTG
Cuts at: 2424 2901 4127 2424
Size: 477 1226 7484
Fragments arranged by size:
7484 1226 477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8194 809
Size: 507 6878 1802
Fragments arranged by size:
6878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2613 3051
Size: 54 1425 50 275 18 195 588 438
Cuts at: 3051 3186 3270 4211 4221 4471 4483 4596 4749
Size: 135 84 941 10 250 12 113 153
Cuts at: 4749 4832 4912 5145 5463 6219 6408 6634 7523
Size: 83 80 233 318 756 189 226 889
Cuts at: 7523 9152 8
Size: 1629 43
Fragments arranged by size:
1629 1425 941 889 756 588 438 318
275 250 233 226 195 189 153 135
113 84 83 80 54 50 43 18
12 10
EagI C'GGCC_G
Cuts at: 1537 1812 4483 4596 4832 6219 6634 1537
Size: 275 2671 113 236 1387 415 4090
Fragments arranged by size:
4090 2671 1387 415 275 236 113
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 3075 5003 5943 7117 1353
Size: 1070 652 1928 940 1174 3423
Fragments arranged by size:
3423 1928 1174 1070 940 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2156 2291 2585 2713 2834 3004
Size: 1064 138 567 135 294 128 121 170
Cuts at: 3004 3660 4562 7774 8148 8294 387
Size: 656 902 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 902 656 567 374 294
170 146 138 135 128 121
EcoICRI GAG'CTC
Cuts at: 2443 2988 4576 4723 5013 5620 2443
Size: 545 1588 147 290 607 6010
Fragments arranged by size:
6010 1588 607 545 290 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2780 2939 2966 2984 3023 3056
Size: 393 434 714 159 27 18 39 33
Cuts at: 3056 3074 3142 3216 3272 4133 4178 4670 4739
Size: 18 68 74 56 861 45 492 69
Cuts at: 4739 5060 5062 5333 5417 5837 5867 6215 6369
Size: 321 2 271 84 420 30 348 154
Cuts at: 6369 6488 7250 7847 8634 8902 1239
Size: 119 762 597 787 268 1524
Fragments arranged by size:
1524 861 787 762 714 597 492 434
420 393 348 321 271 268 159 154
119 84 74 69 68 56 45 39
33 30 27 18 18 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3176 3366 3954 4115 4802 3176
Size: 190 588 161 687 7561
Fragments arranged by size:
7561 687 588 190 161
EcoO109I rG'GnC_Cy
Cuts at: 2584 2870 2909 3416 3428 3656 4242 4243 4279
Size: 286 39 507 12 228 586 1 36
Cuts at: 4279 4280 4510 4968 5119 5197 5506 5682 5715
Size: 1 230 458 151 78 309 176 33
Cuts at: 5715 5750 5860 5861 5999 6063 6463 2584
Size: 35 110 1 138 64 400 5308
Fragments arranged by size:
5308 586 507 458 400 309 286 230
228 176 151 138 110 78 64 39
36 35 33 12 1 1 1
EcoRI G'AATT_C
Cuts at: 6496 6874 6496
Size: 378 8809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 3241 3862 2908
Size: 333 621 8233
Fragments arranged by size:
8233 621 333
FspI TGC'GCA
Cuts at: 7693 7693
Size: 9187
HaeII r_GCGC'y
Cuts at: 1678 2663 3564 3896 4154 4175 4295 6303 8334
Size: 985 901 332 258 21 120 2008 2031
Cuts at: 8334 1678
Size: 2531
Fragments arranged by size:
2531 2031 2008 985 901 332 258 120
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2459 2491 2705
Size: 32 135 32 628 32 227 32 214
Cuts at: 2705 2737 2996 3028 3371 3403 3912 3944 3995
Size: 32 259 32 343 32 509 32 51
Cuts at: 3995 4027 4199 4231 4811 4843 5156 5188 5264
Size: 32 172 32 580 32 313 32 76
Cuts at: 5264 5296 5441 5473 6083 6115 6397 6429 7828
Size: 32 145 32 610 32 282 32 1399
Cuts at: 7828 7860 7902 7934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1399 628 610 580 509 343 313
282 259 227 214 172 145 135 76
51 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 6531 6777 669
Size: 988 281 895 3698 246 3079
Fragments arranged by size:
3698 3079 988 895 281 246
HindIII A'AGCT_T
Cuts at: 748 2230 748
Size: 1482 7705
HpaI GTT'AAC
Cuts at: 6777 6777
Size: 9187
KasI G'GCGC_C
Cuts at: 3560 4171 4291 6299 3560
Size: 611 120 2008 6448
Fragments arranged by size:
6448 2008 611 120
KpnI G_GTAC'C
Cuts at: 6512 6512
Size: 9187
MfeI C'AATT_G
Cuts at: 6786 6786
Size: 9187
MluI A'CGCG_T
Cuts at: 9063 9063
Size: 9187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3852 4419 8301 8485 1305
Size: 2547 567 3882 184 2007
Fragments arranged by size:
3882 2547 2007 567 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3188 3272 4223 4473 4751
Size: 54 1425 343 1356 84 951 250 278
Cuts at: 4751 5147 6410 10
Size: 396 1263 2787
Fragments arranged by size:
2787 1425 1356 1263 951 396 343 278
250 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3854 3908 4883 6363 7145 7504 7663
Size: 648 2688 54 975 1480 782 359 159
Cuts at: 7663 518
Size: 2042
Fragments arranged by size:
2688 2042 1480 975 782 648 359 159
54
MspA1I CmG'CkG
Cuts at: 1606 2794 2821 3629 3679 3718 3742 4051 4275
Size: 1188 27 808 50 39 24 309 224
Cuts at: 4275 4401 4500 4669 4891 5224 5344 5587 6391
Size: 126 99 169 222 333 120 243 804
Cuts at: 6391 6603 7280 8428 8673 1606
Size: 212 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 808 804 677 333 309
245 243 224 222 212 169 126 120
99 50 39 27 24
NaeI GCC'GGC
Cuts at: 4369 4369
Size: 9187
NarI GG'CG_CC
Cuts at: 3561 4172 4292 6300 3561
Size: 611 120 2008 6448
Fragments arranged by size:
6448 2008 611 120
NcoI C'CATG_G
Cuts at: 513 1065 2022 4841 513
Size: 552 957 2819 4859
Fragments arranged by size:
4859 2819 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9187
NgoMIV G'CCGG_C
Cuts at: 4367 4367
Size: 9187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9187
NotI GC'GGCC_GC
Cuts at: 6634 6634
Size: 9187
NspI r_CATG'y
Cuts at: 1132 1229 4699 4825 4888 5341 6545 8090 1132
Size: 97 3470 126 63 453 1204 1545 2229
Fragments arranged by size:
3470 2229 1545 1204 453 126 97 63
PasI CC'CwG_GG
Cuts at: 1348 2866 3178 4176 5123 1348
Size: 1518 312 998 947 5412
Fragments arranged by size:
5412 1518 998 947 312
PciI A'CATG_T
Cuts at: 4695 8086 4695
Size: 3391 5796
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3184 3813 4921 5295 5368 1108
Size: 2076 629 1108 374 73 4927
Fragments arranged by size:
4927 2076 1108 629 374 73
PfoI T'CCnGG_A
Cuts at: 2811 4110 5133 5684 5752 2811
Size: 1299 1023 551 68 6246
Fragments arranged by size:
6246 1299 1023 551 68
PmeI GTTT'AAAC
Cuts at: 6491 6491
Size: 9187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6200 6232 6461 6493 7256 7288 8769 8801 6200
Size: 32 229 32 763 32 1481 32 6586
Fragments arranged by size:
6586 1481 763 229 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4968 5197 5506 5682 5715 5750 5999 4968
Size: 229 309 176 33 35 249 8156
Fragments arranged by size:
8156 309 249 229 176 35 33
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9187
PsiI TTA'TAA
Cuts at: 6757 6757
Size: 9187
PspOMI G'GGCC_C
Cuts at: 2486 2870 2908 3415 3656 4242 4279 5860 6062
Size: 384 38 507 241 586 37 1581 202
Cuts at: 6062 2486
Size: 5611
Fragments arranged by size:
5611 1581 586 507 384 241 202 38
37
PstI C_TGCA'G
Cuts at: 830 2373 2559 2997 3170 5499 5523 6191 6539
Size: 1543 186 438 173 2329 24 668 348
Cuts at: 6539 830
Size: 3478
Fragments arranged by size:
3478 2329 1543 668 438 348 186 173
24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9187
PvuII CAG'CTG
Cuts at: 2821 3679 3718 4891 5344 5587 2821
Size: 858 39 1173 453 243 6421
Fragments arranged by size:
6421 1173 858 453 243 39
SacI G_AGCT'C
Cuts at: 2445 2990 4578 4725 5015 5622 2445
Size: 545 1588 147 290 607 6010
Fragments arranged by size:
6010 1588 607 545 290 147
SacII CC_GC'GG
Cuts at: 3630 4402 4501 3630
Size: 772 99 8316
Fragments arranged by size:
8316 772 99
SalI G'TCGA_C
Cuts at: 1936 6529 1936
Size: 4593 4594
SanDI GG'GwC_CC
Cuts at: 5715 5715
Size: 9187
SapI GCTCTTCn'nnn_
Cuts at: 5003 5003
Size: 9187
SbfI CC_TGCA'GG
Cuts at: 6539 6539
Size: 9187
ScaI AGT'ACT
Cuts at: 7435 9147 7435
Size: 1712 7475
SfcI C'TryA_G
Cuts at: 826 1045 2369 2555 2993 3166 5495 5519 5677
Size: 219 1324 186 438 173 2329 24 158
Cuts at: 5677 6187 6535 7670 8351 8542 826
Size: 510 348 1135 681 191 1471
Fragments arranged by size:
2329 1471 1324 1135 681 510 438 348
219 191 186 173 158 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2930 2930
Size: 9187
SfoI GGC'GCC
Cuts at: 3562 4173 4293 6301 3562
Size: 611 120 2008 6448
Fragments arranged by size:
6448 2008 611 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9187
SmaI CCC'GGG
Cuts at: 1872 2527 3400 3661 5537 6061 6514 1872
Size: 655 873 261 1876 524 453 4545
Fragments arranged by size:
4545 1876 873 655 524 453 261
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4577 5642 7291 8192 8454 820
Size: 197 926 2634 1065 1649 901 262 1553
Fragments arranged by size:
2634 1649 1553 1065 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6484 6978 493
Size: 5991 494 2702
Fragments arranged by size:
5991 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9187
SphI G_CATG'C
Cuts at: 1132 4888 5341 6545 1132
Size: 3756 453 1204 3774
Fragments arranged by size:
3774 3756 1204 453
SspI AAT'ATT
Cuts at: 5 52 2054 7111 5
Size: 47 2002 5057 2081
Fragments arranged by size:
5057 2081 2002 47
StuI AGG'CCT
Cuts at: 1517 3224 5579 6237 1517
Size: 1707 2355 658 4467
Fragments arranged by size:
4467 2355 1707 658
StyI C'CwwG_G
Cuts at: 513 1065 2022 2724 2937 4251 4841 4964 5995
Size: 552 957 702 213 1314 590 123 1031
Cuts at: 5995 6369 6626 513
Size: 374 257 3074
Fragments arranged by size:
3074 1314 1031 957 702 590 552 374
257 213 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 3772 5796 5906 7214 7231 7384
Size: 1255 933 1259 2024 110 1308 17 153
Cuts at: 7384 7569 8014 325
Size: 185 445 1498
Fragments arranged by size:
2024 1498 1308 1259 1255 933 445 185
153 110 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2596 3886 4692
Size: 274 80 33 51 1443 619 1290 806
Cuts at: 4692 5103 7433 9145 96
Size: 411 2330 1712 138
Fragments arranged by size:
2330 1712 1443 1290 806 619 411 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4376 4408 9147 9179 4376
Size: 32 4739 32 4384
Fragments arranged by size:
4739 4384 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9187
XbaI T'CTAG_A
Cuts at: 3985 6523 3985
Size: 2538 6649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2873 3073 4039 6340 2873
Size: 200 966 2301 5720
Fragments arranged by size:
5720 2301 966 200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9187
XmaI C'CCGG_G
Cuts at: 1870 2525 3398 3659 5535 6059 6512 1870
Size: 655 873 261 1876 524 453 4545
Fragments arranged by size:
4545 1876 873 655 524 453 261
XmnI GAAnn'nnTTC
Cuts at: 3863 7316 3863
Size: 3453 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2475 6995 276
Size: 53 83 186 936 941 4520 2468
Fragments arranged by size:
4520 2468 941 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SexAI SrfI SwaI