Restriction map for
pFN21ASDA0286
(Circular) (Six-base) MAPSORT of: pFN21ASDA0286.seq Check: 8269 from: 1 to: 6061
pFN21ASDA0286 6061 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3005 3005
Size: 6061
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 3404 1937
Size: 202 1265 4594
Fragments arranged by size:
4594 1265 202
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2354 4124 5508 5776 1632
Size: 530 192 1770 1384 268 1917
Fragments arranged by size:
1917 1770 1384 530 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2231 2312 1166
Size: 1065 81 4915
Fragments arranged by size:
4915 1065 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 2354
Size: 32 6029
AlwNI CAG_nnn'CTG
Cuts at: 2986 3804 5376 2986
Size: 818 1572 3671
Fragments arranged by size:
3671 1572 818
ApaI G_GGCC'C
Cuts at: 2073 2832 2073
Size: 759 5302
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2574 2734 3113 3370 3596 3748 5857 1493
Size: 1081 160 379 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1081 379 257 226 160 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2080 3386 1870
Size: 73 123 14 1306 4545
Fragments arranged by size:
4545 1306 123 73 14
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 4837 618
Size: 325 217 627 1595 1455 1842
Fragments arranged by size:
1842 1595 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2073 2081 2832 3108 3380 1391
Size: 682 8 751 276 272 4072
Fragments arranged by size:
4072 751 682 276 272 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3152 928
Size: 971 1253 3837
Fragments arranged by size:
3837 1253 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3060 3953 5163 2262
Size: 798 893 1210 3160
Fragments arranged by size:
3160 1210 893 798
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2745 6056 2745
Size: 3311 2750
BlpI GC'TnA_GC
Cuts at: 2561 3478 2561
Size: 917 5144
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2832 4122 5278 1790
Size: 283 759 1290 1156 2573
Fragments arranged by size:
2573 1290 1156 759 283
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2601 3792 4750
Size: 416 53 83 399 24 1184 1191 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1191 1184 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2770 4721 1239
Size: 1531 1951 2579
Fragments arranged by size:
2579 1951 1531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 4724 882
Size: 617 554 2671 2219
Fragments arranged by size:
2671 2219 617 554
BsaAI yAC'GTr
Cuts at: 493 1204 3358 3852 493
Size: 711 2154 494 2702
Fragments arranged by size:
2702 2154 711 494
BsaBI GATnn'nnATC
Cuts at: 2273 6055 2273
Size: 3782 2279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2490 4494 4892 5166 5313 5724 2490
Size: 2004 398 274 147 411 2827
Fragments arranged by size:
2827 2004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 3116 3314 3317 1186
Size: 1153 777 198 3 3930
Fragments arranged by size:
3930 1153 777 198 3
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3108 4122 4207 5278 3108
Size: 1014 85 1071 3891
Fragments arranged by size:
3891 1071 1014 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
BsmBI CGTCTCn'nnnn_
Cuts at: 3047 3047
Size: 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2081 2832 3108 3380 4122 4207
Size: 399 283 8 751 276 272 742 85
Cuts at: 4207 5278 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 751 742 399 283 276 272
85 8
BspEI T'CCGG_A
Cuts at: 2490 5724 2490
Size: 3234 2827
BspHI T'CATG_A
Cuts at: 2799 3950 5680 2799
Size: 1151 1730 3180
Fragments arranged by size:
3180 1730 1151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2444 2865 3005 3416 844
Size: 1600 421 140 411 3489
Fragments arranged by size:
3489 1600 421 411 140
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 4556 4730 65
Size: 1183 1695 1613 174 1396
Fragments arranged by size:
1695 1613 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 4705 4892 1700
Size: 110 243 2652 187 2869
Fragments arranged by size:
2869 2652 243 187 110
BsrGI T'GTAC_A
Cuts at: 96 2988 96
Size: 2892 3169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 2007 4865 2007
Size: 2858 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2274 2745 3391 3758 4143 4160 5612 5698
Size: 302 471 646 367 385 17 1452 86
Cuts at: 5698 5710 6056 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 646 471 385 367 346 302
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4552
Fragments arranged by size:
4552 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4371 4391 3577
Size: 794 20 5247
Fragments arranged by size:
5247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 2382 2731 3365 2382
Size: 349 634 5078
Fragments arranged by size:
5078 634 349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026
Size: 54 1425 50 275 18 1678 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1678 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3205 3991 1353
Size: 1852 786 3423
Fragments arranged by size:
3423 1852 786
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3106 3106
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2354 2770 4124 4721 5508 5776
Size: 393 530 192 416 1354 597 787 268
Cuts at: 5776 1239
Size: 1524
Fragments arranged by size:
1524 1354 787 597 530 416 393 268
192
EcoO109I rG'GnC_Cy
Cuts at: 2070 2829 2070
Size: 759 5302
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 2554 2586 3139
Size: 32 135 32 913 32 37 32 553
Cuts at: 3139 3171 3275 3307 4702 4734 4776 4808 1373
Size: 32 104 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1395 913 553 135 104 42 37
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 3405 3651 669
Size: 988 281 472 995 246 3079
Fragments arranged by size:
3079 995 988 472 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 2410 3651 2410
Size: 1241 4820
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2773 5175 5359 1305
Size: 1468 2402 184 2007
Fragments arranged by size:
2402 2007 1468 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2312 2684 4019 4378 4537 518
Size: 648 1065 81 372 1335 359 159 2042
Fragments arranged by size:
2042 1335 1065 648 372 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2150 2878 3477 4154 5302 5547 1606
Size: 544 728 599 677 1148 245 2120
Fragments arranged by size:
2120 1148 728 677 599 544 245
NaeI GCC'GGC
Cuts at: 2055 2055
Size: 6061
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4552
Fragments arranged by size:
4552 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NgoMIV G'CCGG_C
Cuts at: 2053 2053
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2073 1348
Size: 725 5336
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2069 2828 2069
Size: 759 5302
PstI C_TGCA'G
Cuts at: 830 3002 3413 830
Size: 2172 411 3478
Fragments arranged by size:
3478 2172 411
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2150 2878 2150
Size: 728 5333
SacI G_AGCT'C
Cuts at: 3108 3108
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SapI GCTCTTCn'nnn_
Cuts at: 3205 3205
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2998 3409 4544 5225 5416 826
Size: 219 1953 411 1135 681 191 1471
Fragments arranged by size:
1953 1471 1135 681 411 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 2068 3388 1872
Size: 196 1320 4545
Fragments arranged by size:
4545 1320 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3358 3852 493
Size: 2865 494 2702
Fragments arranged by size:
2865 2702 494
SpeI A'CTAG_T
Cuts at: 152 2722 152
Size: 2570 3491
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2022 2832 3500 513
Size: 552 957 810 668 3074
Fragments arranged by size:
3074 957 810 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 4088 4105 4258 4443 4888 325
Size: 1255 744 1764 17 153 185 445 1498
Fragments arranged by size:
1764 1498 1255 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2954 2988
Size: 274 80 33 51 1443 495 482 34
Cuts at: 2988 4307 6019 96
Size: 1319 1712 138
Fragments arranged by size:
1712 1443 1319 495 482 274 138 80
51 34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2057 6021 6053 2025
Size: 32 3964 32 2033
Fragments arranged by size:
3964 2033 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 2066 3386 1870
Size: 196 1320 4545
Fragments arranged by size:
4545 1320 196
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI