Restriction map for
pFN21ASDA0278
(Circular) (Six-base) MAPSORT of: pFN21ASDA0278.seq Check: 650 from: 1 to: 5917
pFN21ASDA0278 5917 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 2175 2179 2701 2074
Size: 101 4 522 5290
Fragments arranged by size:
5290 522 101 4
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3980 5364 5632 1632
Size: 593 1755 1384 268 1917
Fragments arranged by size:
1917 1755 1384 593 268
AfeI AGC'GCT
Cuts at: 2279 2342 2396 2279
Size: 63 54 5800
Fragments arranged by size:
5800 63 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2364 4816 5793 1203
Size: 1161 2452 977 1327
Fragments arranged by size:
2452 1327 1161 977
AgeI A'CCGG_T
Cuts at: 2217 4748 2217
Size: 2531 3386
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3101 1184
Size: 1917 4000
AlwNI CAG_nnn'CTG
Cuts at: 2561 2926 3660 5232 2561
Size: 365 734 1572 3246
Fragments arranged by size:
3246 1572 734 365
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2486 2573 2673 2862 3206 3242
Size: 73 123 420 87 100 189 344 36
Cuts at: 3242 1870
Size: 4545
Fragments arranged by size:
4545 420 344 189 123 100 87 73
36
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 3002 3238 4693 618
Size: 325 217 627 376 839 236 1455 1842
Fragments arranged by size:
1842 1455 839 627 376 325 236 217
BanII G_rGCy'C
Cuts at: 1391 2055 2586 2801 3236 1391
Size: 664 531 215 435 4072
Fragments arranged by size:
4072 664 531 435 215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3116 4528 136
Size: 107 122 71 631 605 1444 1412 1525
Fragments arranged by size:
1525 1444 1412 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2658 5912 2658
Size: 3254 2663
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2855 3005 3978 5134 1790
Size: 472 593 150 973 1156 2573
Fragments arranged by size:
2573 1156 973 593 472 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2821 3648 4606
Size: 416 53 83 399 24 1404 827 958
Cuts at: 4606 442
Size: 1753
Fragments arranged by size:
1753 1404 958 827 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2255 2357 2411 2432 3077 3119 4577
Size: 920 96 102 54 21 645 42 1458
Cuts at: 4577 1239
Size: 2579
Fragments arranged by size:
2579 1458 920 645 102 96 54 42
21
Bpu10I CC'TnA_GC
Cuts at: 2684 2684
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 4042 4907 5205 3005
Size: 1037 865 298 3717
Fragments arranged by size:
3717 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 3146 4580 882
Size: 617 816 831 1434 2219
Fragments arranged by size:
2219 1434 831 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 2146 2257 2365 2962 2995 3214 3708
Size: 711 942 111 108 597 33 219 494
Cuts at: 3708 493
Size: 2702
Fragments arranged by size:
2702 942 711 597 494 219 111 108
33
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2037 2217 3195 4350 4748 5022 5169 5580 2037
Size: 180 978 1155 398 274 147 411 2374
Fragments arranged by size:
2374 1155 978 411 398 274 180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 2741 2990 2993 1186
Size: 1525 30 249 3 4110
Fragments arranged by size:
4110 1525 249 30 3
BseYI C'CCAG_C
Cuts at: 2343 2397 2554 2595 2636 3201 5120 5616 2343
Size: 54 157 41 41 565 1919 496 2644
Fragments arranged by size:
2644 1919 565 496 157 54 41 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 3107 1163
Size: 573 462 909 3973
Fragments arranged by size:
3973 909 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2222 2226 2407 3111 3120 3367
Size: 275 186 221 4 181 704 9 247
Cuts at: 3367 4128 5156 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 704 275 247 221 186
181 9 4
BsiHKAI G_wGCw'C
Cuts at: 2145 3978 4063 5134 2145
Size: 1833 85 1071 2928
Fragments arranged by size:
2928 1833 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2145 2262 2586 2801 2855 3005
Size: 399 265 90 117 324 215 54 150
Cuts at: 3005 3236 3978 4063 5134 1391
Size: 231 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 265 231 215
150 117 90 85 54
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2175 2179 2701 2898 3272 844
Size: 1230 101 4 522 197 374 3489
Fragments arranged by size:
3489 1230 522 374 197 101 4
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 2217 2767 3115 3119 4561 4748
Size: 110 350 57 550 348 4 1442 187
Cuts at: 4748 1700
Size: 2869
Fragments arranged by size:
2869 1442 550 350 348 187 110 57
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 2263 2501 3977 4989 2263
Size: 238 1476 1012 3191
Fragments arranged by size:
3191 1476 1012 238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 2007 4721 2007
Size: 2714 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2191 3055 2029
Size: 162 864 4891
Fragments arranged by size:
4891 864 162
BstYI r'GATC_y
Cuts at: 1972 2658 2745 3247 3614 3999 4016 5468 5554
Size: 686 87 502 367 385 17 1452 86
Cuts at: 5554 5566 5912 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 686 502 385 367 346 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2072 2418 2469 2609 3183 513
Size: 552 957 50 346 51 140 574 3247
Fragments arranged by size:
3247 957 574 552 346 140 51 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2311 487
Size: 683 56 1085 4093
Fragments arranged by size:
4093 1085 683 56
BtsI GCAGTG_nn'
Cuts at: 3433 4227 4247 3433
Size: 794 20 5103
Fragments arranged by size:
5103 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 4924 809
Size: 507 1209 2399 1802
Fragments arranged by size:
2399 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2172 2769
Size: 54 1425 50 275 18 258 84 597
Cuts at: 2769 3108 3117 3186 3364 4253 5882 8
Size: 339 9 69 178 889 1629 43
Fragments arranged by size:
1629 1425 889 597 339 275 258 178
84 69 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3108 3117 3364 1537
Size: 275 1296 9 247 4090
Fragments arranged by size:
4090 1296 275 247 9
EarI CTCTTCn'nnn_
Cuts at: 1353 3847 1353
Size: 2494 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2167 3164 4504 4878 5024 387
Size: 1064 138 578 997 1340 374 146 1280
Fragments arranged by size:
1340 1280 1064 997 578 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2225 2255 2357 2411 2432 3077
Size: 393 527 66 30 102 54 21 645
Cuts at: 3077 3119 3980 4577 5364 5632 1239
Size: 42 861 597 787 268 1524
Fragments arranged by size:
1524 861 787 645 597 527 393 268
102 66 54 42 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3101 3101
Size: 5917
EcoO109I rG'GnC_Cy
Cuts at: 2483 2614 2668 2941 2483
Size: 131 54 273 5459
Fragments arranged by size:
5459 273 131 54
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FseI GG_CCGG'CC
Cuts at: 2771 3123 2771
Size: 352 5565
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2281 2344 2398 5064 1678
Size: 603 63 54 2666 2531
Fragments arranged by size:
2666 2531 603 63 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2737 2769 2856 2888 4558
Size: 32 135 32 1165 32 87 32 1670
Cuts at: 4558 4590 4632 4664 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1670 1165 135 87 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5031 5215 1305
Size: 3726 184 2007
Fragments arranged by size:
3726 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 3188 10
Size: 54 1425 343 258 1098 2739
Fragments arranged by size:
2739 1425 1098 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 3875 4234 4393 518
Size: 648 1917 792 359 159 2042
Fragments arranged by size:
2042 1917 792 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2074 2313 2561 2878 2914 3333 4010
Size: 440 28 239 248 317 36 419 677
Cuts at: 4010 5158 5403 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 440 419 317 248 245
239 36 28
NaeI GCC'GGC
Cuts at: 2769 3117 3121 2769
Size: 348 4 5565
Fragments arranged by size:
5565 348 4
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4408
Fragments arranged by size:
4408 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2767 3115 3119 2767
Size: 348 4 5565
Fragments arranged by size:
5565 348 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2561 2778 2995 1108
Size: 1453 217 217 4030
Fragments arranged by size:
4030 1453 217 217
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PmlI CAC'GTG
Cuts at: 2146 2365 2146
Size: 219 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2483 2614 2668 2941 2483
Size: 131 54 273 5459
Fragments arranged by size:
5459 273 131 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2112 2841 3012 3269 830
Size: 1282 729 171 257 3478
Fragments arranged by size:
3478 1282 729 257 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
SacII CC_GC'GG
Cuts at: 2075 2075
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SanDI GG'GwC_CC
Cuts at: 2483 2614 2483
Size: 131 5786
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 2702 2732 4165 5877 2702
Size: 30 1433 1712 2742
Fragments arranged by size:
2742 1712 1433 30
SfcI C'TryA_G
Cuts at: 826 1045 2108 2837 3008 3265 4400 5081 5272
Size: 219 1063 729 171 257 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1471 1135 1063 729 681 257 219 191
171
SfiI GGCCn_nnn'nGGCC
Cuts at: 3116 3116
Size: 5917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 2068 2575 3244 1872
Size: 196 507 669 4545
Fragments arranged by size:
4545 669 507 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 3020 4021 4922 5184 820
Size: 197 926 730 347 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 730 347 262 197
SnaBI TAC'GTA
Cuts at: 493 3214 3708 493
Size: 2721 494 2702
Fragments arranged by size:
2721 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SrfI GCCC'GGGC
Cuts at: 2575 2575
Size: 5917
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 2300 1517
Size: 783 5134
StyI C'CwwG_G
Cuts at: 513 1065 2022 3356 513
Size: 552 957 1334 3074
Fragments arranged by size:
3074 1334 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2803 3944 3961 4114 4299 4744
Size: 1255 632 591 1141 17 153 185 445
Cuts at: 4744 325
Size: 1498
Fragments arranged by size:
1498 1255 1141 632 591 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 2730 2841
Size: 274 80 33 51 1443 723 30 111
Cuts at: 2841 4163 5875 96
Size: 1322 1712 138
Fragments arranged by size:
1712 1443 1322 723 274 138 111 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2450 2482 5877 5909 2450
Size: 32 3395 32 2458
Fragments arranged by size:
3395 2458 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2332 2644 2332
Size: 312 5605
XhoI C'TCGA_G
Cuts at: 1943 2673 1943
Size: 730 5187
XmaI C'CCGG_G
Cuts at: 1870 2066 2573 3242 1870
Size: 196 507 669 4545
Fragments arranged by size:
4545 669 507 196
XmnI GAAnn'nnTTC
Cuts at: 2831 4046 2831
Size: 1215 4702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PfoI
PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI
SfoI SwaI