(Circular) (Six-base) MAPSORT of: pFN21ASDA0278.seq Check: 650 from: 1 to: 5917 pFN21ASDA0278 5917 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2074 2175 2179 2701 2074 Size: 101 4 522 5290 Fragments arranged by size: 5290 522 101 4 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 3980 5364 5632 1632 Size: 593 1755 1384 268 1917 Fragments arranged by size: 1917 1755 1384 593 268 AfeI AGC'GCT Cuts at: 2279 2342 2396 2279 Size: 63 54 5800 Fragments arranged by size: 5800 63 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2364 4816 5793 1203 Size: 1161 2452 977 1327 Fragments arranged by size: 2452 1327 1161 977 AgeI A'CCGG_T Cuts at: 2217 4748 2217 Size: 2531 3386 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3101 1184 Size: 1917 4000 AlwNI CAG_nnn'CTG Cuts at: 2561 2926 3660 5232 2561 Size: 365 734 1572 3246 Fragments arranged by size: 3246 1572 734 365 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2486 2573 2673 2862 3206 3242 Size: 73 123 420 87 100 189 344 36 Cuts at: 3242 1870 Size: 4545 Fragments arranged by size: 4545 420 344 189 123 100 87 73 36 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2163 3002 3238 4693 618 Size: 325 217 627 376 839 236 1455 1842 Fragments arranged by size: 1842 1455 839 627 376 325 236 217 BanII G_rGCy'C Cuts at: 1391 2055 2586 2801 3236 1391 Size: 664 531 215 435 4072 Fragments arranged by size: 4072 664 531 435 215 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3116 4528 136 Size: 107 122 71 631 605 1444 1412 1525 Fragments arranged by size: 1525 1444 1412 631 605 122 107 71 BglII A'GATC_T Cuts at: 2658 5912 2658 Size: 3254 2663 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2262 2855 3005 3978 5134 1790 Size: 472 593 150 973 1156 2573 Fragments arranged by size: 2573 1156 973 593 472 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2821 3648 4606 Size: 416 53 83 399 24 1404 827 958 Cuts at: 4606 442 Size: 1753 Fragments arranged by size: 1753 1404 958 827 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2255 2357 2411 2432 3077 3119 4577 Size: 920 96 102 54 21 645 42 1458 Cuts at: 4577 1239 Size: 2579 Fragments arranged by size: 2579 1458 920 645 102 96 54 42 21 Bpu10I CC'TnA_GC Cuts at: 2684 2684 Size: 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 4042 4907 5205 3005 Size: 1037 865 298 3717 Fragments arranged by size: 3717 1037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 3146 4580 882 Size: 617 816 831 1434 2219 Fragments arranged by size: 2219 1434 831 816 617 BsaAI yAC'GTr Cuts at: 493 1204 2146 2257 2365 2962 2995 3214 3708 Size: 711 942 111 108 597 33 219 494 Cuts at: 3708 493 Size: 2702 Fragments arranged by size: 2702 942 711 597 494 219 111 108 33 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2037 2217 3195 4350 4748 5022 5169 5580 2037 Size: 180 978 1155 398 274 147 411 2374 Fragments arranged by size: 2374 1155 978 411 398 274 180 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2711 2741 2990 2993 1186 Size: 1525 30 249 3 4110 Fragments arranged by size: 4110 1525 249 30 3 BseYI C'CCAG_C Cuts at: 2343 2397 2554 2595 2636 3201 5120 5616 2343 Size: 54 157 41 41 565 1919 496 2644 Fragments arranged by size: 2644 1919 565 496 157 54 41 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 3107 1163 Size: 573 462 909 3973 Fragments arranged by size: 3973 909 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2222 2226 2407 3111 3120 3367 Size: 275 186 221 4 181 704 9 247 Cuts at: 3367 4128 5156 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 704 275 247 221 186 181 9 4 BsiHKAI G_wGCw'C Cuts at: 2145 3978 4063 5134 2145 Size: 1833 85 1071 2928 Fragments arranged by size: 2928 1833 1071 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2145 2262 2586 2801 2855 3005 Size: 399 265 90 117 324 215 54 150 Cuts at: 3005 3236 3978 4063 5134 1391 Size: 231 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 265 231 215 150 117 90 85 54 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2175 2179 2701 2898 3272 844 Size: 1230 101 4 522 197 374 3489 Fragments arranged by size: 3489 1230 522 374 197 101 4 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 2217 2767 3115 3119 4561 4748 Size: 110 350 57 550 348 4 1442 187 Cuts at: 4748 1700 Size: 2869 Fragments arranged by size: 2869 1442 550 350 348 187 110 57 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 2263 2501 3977 4989 2263 Size: 238 1476 1012 3191 Fragments arranged by size: 3191 1476 1012 238 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 2007 4721 2007 Size: 2714 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2191 3055 2029 Size: 162 864 4891 Fragments arranged by size: 4891 864 162 BstYI r'GATC_y Cuts at: 1972 2658 2745 3247 3614 3999 4016 5468 5554 Size: 686 87 502 367 385 17 1452 86 Cuts at: 5554 5566 5912 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 686 502 385 367 346 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2072 2418 2469 2609 3183 513 Size: 552 957 50 346 51 140 574 3247 Fragments arranged by size: 3247 957 574 552 346 140 51 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2311 487 Size: 683 56 1085 4093 Fragments arranged by size: 4093 1085 683 56 BtsI GCAGTG_nn' Cuts at: 3433 4227 4247 3433 Size: 794 20 5103 Fragments arranged by size: 5103 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 4924 809 Size: 507 1209 2399 1802 Fragments arranged by size: 2399 1802 1209 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2172 2769 Size: 54 1425 50 275 18 258 84 597 Cuts at: 2769 3108 3117 3186 3364 4253 5882 8 Size: 339 9 69 178 889 1629 43 Fragments arranged by size: 1629 1425 889 597 339 275 258 178 84 69 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3108 3117 3364 1537 Size: 275 1296 9 247 4090 Fragments arranged by size: 4090 1296 275 247 9 EarI CTCTTCn'nnn_ Cuts at: 1353 3847 1353 Size: 2494 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2167 3164 4504 4878 5024 387 Size: 1064 138 578 997 1340 374 146 1280 Fragments arranged by size: 1340 1280 1064 997 578 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2225 2255 2357 2411 2432 3077 Size: 393 527 66 30 102 54 21 645 Cuts at: 3077 3119 3980 4577 5364 5632 1239 Size: 42 861 597 787 268 1524 Fragments arranged by size: 1524 861 787 645 597 527 393 268 102 66 54 42 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 3101 3101 Size: 5917 EcoO109I rG'GnC_Cy Cuts at: 2483 2614 2668 2941 2483 Size: 131 54 273 5459 Fragments arranged by size: 5459 273 131 54 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FseI GG_CCGG'CC Cuts at: 2771 3123 2771 Size: 352 5565 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2281 2344 2398 5064 1678 Size: 603 63 54 2666 2531 Fragments arranged by size: 2666 2531 603 63 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2737 2769 2856 2888 4558 Size: 32 135 32 1165 32 87 32 1670 Cuts at: 4558 4590 4632 4664 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1670 1165 135 87 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5031 5215 1305 Size: 3726 184 2007 Fragments arranged by size: 3726 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 3188 10 Size: 54 1425 343 258 1098 2739 Fragments arranged by size: 2739 1425 1098 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 3083 3875 4234 4393 518 Size: 648 1917 792 359 159 2042 Fragments arranged by size: 2042 1917 792 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2074 2313 2561 2878 2914 3333 4010 Size: 440 28 239 248 317 36 419 677 Cuts at: 4010 5158 5403 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 440 419 317 248 245 239 36 28 NaeI GCC'GGC Cuts at: 2769 3117 3121 2769 Size: 348 4 5565 Fragments arranged by size: 5565 348 4 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4408 Fragments arranged by size: 4408 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NgoMIV G'CCGG_C Cuts at: 2767 3115 3119 2767 Size: 348 4 5565 Fragments arranged by size: 5565 348 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3275 4820 1132 Size: 97 2046 1545 2229 Fragments arranged by size: 2229 2046 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 2561 2778 2995 1108 Size: 1453 217 217 4030 Fragments arranged by size: 4030 1453 217 217 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PmlI CAC'GTG Cuts at: 2146 2365 2146 Size: 219 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2483 2614 2668 2941 2483 Size: 131 54 273 5459 Fragments arranged by size: 5459 273 131 54 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PstI C_TGCA'G Cuts at: 830 2112 2841 3012 3269 830 Size: 1282 729 171 257 3478 Fragments arranged by size: 3478 1282 729 257 171 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 SacII CC_GC'GG Cuts at: 2075 2075 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SanDI GG'GwC_CC Cuts at: 2483 2614 2483 Size: 131 5786 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 2702 2732 4165 5877 2702 Size: 30 1433 1712 2742 Fragments arranged by size: 2742 1712 1433 30 SfcI C'TryA_G Cuts at: 826 1045 2108 2837 3008 3265 4400 5081 5272 Size: 219 1063 729 171 257 1135 681 191 Cuts at: 5272 826 Size: 1471 Fragments arranged by size: 1471 1135 1063 729 681 257 219 191 171 SfiI GGCCn_nnn'nGGCC Cuts at: 3116 3116 Size: 5917 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 2068 2575 3244 1872 Size: 196 507 669 4545 Fragments arranged by size: 4545 669 507 196 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 3020 4021 4922 5184 820 Size: 197 926 730 347 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 730 347 262 197 SnaBI TAC'GTA Cuts at: 493 3214 3708 493 Size: 2721 494 2702 Fragments arranged by size: 2721 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SrfI GCCC'GGGC Cuts at: 2575 2575 Size: 5917 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 2300 1517 Size: 783 5134 StyI C'CwwG_G Cuts at: 513 1065 2022 3356 513 Size: 552 957 1334 3074 Fragments arranged by size: 3074 1334 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2212 2803 3944 3961 4114 4299 4744 Size: 1255 632 591 1141 17 153 185 445 Cuts at: 4744 325 Size: 1498 Fragments arranged by size: 1498 1255 1141 632 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 2730 2841 Size: 274 80 33 51 1443 723 30 111 Cuts at: 2841 4163 5875 96 Size: 1322 1712 138 Fragments arranged by size: 1712 1443 1322 723 274 138 111 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2450 2482 5877 5909 2450 Size: 32 3395 32 2458 Fragments arranged by size: 3395 2458 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2332 2644 2332 Size: 312 5605 XhoI C'TCGA_G Cuts at: 1943 2673 1943 Size: 730 5187 XmaI C'CCGG_G Cuts at: 1870 2066 2573 3242 1870 Size: 196 507 669 4545 Fragments arranged by size: 4545 669 507 196 XmnI GAAnn'nnTTC Cuts at: 2831 4046 2831 Size: 1215 4702 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfoI SwaI