Restriction map for
pFN21ASDA0273
(Circular) (Six-base) MAPSORT of: pFN21ASDA0273.seq Check: 9126 from: 1 to: 5719
pFN21ASDA0273 5719 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2078 2078
Size: 5719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2193 3529 278
Size: 53 83 186 936 657 1336 2468
Fragments arranged by size:
2468 1336 936 657 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2898 2954 3062 1937
Size: 961 56 108 4594
Fragments arranged by size:
4594 961 108 56
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2534 2557 3782 5166 5434 1632
Size: 743 159 23 1225 1384 268 1917
Fragments arranged by size:
1917 1384 1225 743 268 159 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 2164 3028 3254 3406 5515 1493
Size: 671 864 226 152 2109 1697
Fragments arranged by size:
2109 1697 864 671 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 1870
Size: 73 1101 4545
Fragments arranged by size:
4545 1101 73
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2740 2912 2934 3040 4495
Size: 325 217 627 953 172 22 106 1455
Cuts at: 4495 618
Size: 1842
Fragments arranged by size:
1842 1455 953 627 325 217 172 106
22
BanII G_rGCy'C
Cuts at: 1391 2261 2895 3038 1391
Size: 870 634 143 4072
Fragments arranged by size:
4072 870 634 143
BbeI G_GCGC'C
Cuts at: 2744 2744
Size: 5719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2431 2560 2839 928
Size: 971 532 129 279 3808
Fragments arranged by size:
3808 971 532 279 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 2385 2403 2652 3136 2385
Size: 18 249 484 4968
Fragments arranged by size:
4968 484 249 18
Bme1580I G_kGCm'C
Cuts at: 1790 2915 2937 3780 4936 1790
Size: 1125 22 843 1156 2573
Fragments arranged by size:
2573 1156 1125 843 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2367 3450 4408
Size: 416 53 83 399 24 950 1083 958
Cuts at: 4408 442
Size: 1753
Fragments arranged by size:
1753 1083 958 950 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2353 2894 4379 1239
Size: 896 218 541 1485 2579
Fragments arranged by size:
2579 1485 896 541 218
Bpu10I CC'TnA_GC
Cuts at: 2255 2255
Size: 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3016 3510 493
Size: 711 1812 494 2702
Fragments arranged by size:
2702 1812 711 494
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 2741 3526 3908
Size: 53 83 186 936 657 551 785 382
Cuts at: 3908 4543 275
Size: 635 1451
Fragments arranged by size:
1451 936 785 657 635 551 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 1178
Size: 30 165 30 615 30 4849
Fragments arranged by size:
4849 615 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5719
BseYI C'CCAG_C
Cuts at: 2647 2669 2978 4922 5418 2647
Size: 22 309 1944 496 2948
Fragments arranged by size:
2948 1944 496 309 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2338 1163
Size: 573 570 32 4544
Fragments arranged by size:
4544 573 570 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2629 2779 3169 3930 4958 1540
Size: 275 186 628 150 390 761 1028 2301
Fragments arranged by size:
2301 1028 761 628 390 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 2379 3780 3865 4936 2379
Size: 1401 85 1071 3162
Fragments arranged by size:
3162 1401 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
BsmBI CGTCTCn'nnnn_
Cuts at: 2333 2333
Size: 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2379 2895 2915 2937 3038 3780
Size: 399 471 118 516 20 22 101 742
Cuts at: 3780 3865 4936 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 471 399 118 101
85 22 20
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 2943 3608 5338 2943
Size: 665 1730 3324
Fragments arranged by size:
3324 1730 665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 2828 2999 3074 844
Size: 1234 750 171 75 3489
Fragments arranged by size:
3489 1234 750 171 75
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2796 4214 4388 65
Size: 1183 1548 1418 174 1396
Fragments arranged by size:
1548 1418 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 2204 3779 4791 2204
Size: 1575 1012 3132
Fragments arranged by size:
3132 1575 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2991 794
Size: 2197 3522
BstBI TT'CG_AA
Cuts at: 2007 4523 2007
Size: 2516 3203
BstEII G'GTnAC_C
Cuts at: 1184 2122 1184
Size: 938 4781
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2206
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2643 3049 3416 3801 3818 5270 5356 5368
Size: 671 406 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 671 406 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2097 2533 2560 513
Size: 552 957 75 436 27 3672
Fragments arranged by size:
3672 957 552 436 75 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2322 487
Size: 683 56 1096 3884
Fragments arranged by size:
3884 1096 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 2223 2301 2485 3235 4029 4049 2222
Size: 1 78 184 750 794 20 3892
Fragments arranged by size:
3892 794 750 184 78 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2280 2626
Size: 54 1425 50 275 18 378 72 346
Cuts at: 2626 3166 4055 5684 8
Size: 540 889 1629 43
Fragments arranged by size:
1629 1425 889 540 378 346 275 72
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2626 3166 1537
Size: 275 814 540 4090
Fragments arranged by size:
4090 814 540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3649 1353
Size: 1313 983 3423
Fragments arranged by size:
3423 1313 983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2840 4306 4680 4826 387
Size: 1064 138 1251 1466 374 146 1280
Fragments arranged by size:
1466 1280 1251 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2353 2375 2534 2557 2894 3782
Size: 393 503 218 22 159 23 337 888
Cuts at: 3782 4379 5166 5434 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 503 393 337 268
218 159 23 22
EcoO109I rG'GnC_Cy
Cuts at: 2481 2863 2974 2481
Size: 382 111 5226
Fragments arranged by size:
5226 382 111
EcoRI G'AATT_C
Cuts at: 2164 3028 3406 2164
Size: 864 378 4477
Fragments arranged by size:
4477 864 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2638 2659 2744 4866 1678
Size: 960 21 85 2122 2531
Fragments arranged by size:
2531 2122 960 85 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2635 2667 4360
Size: 32 135 32 446 32 585 32 1693
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1693 585 446 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2899 3063 3309 669
Size: 988 281 961 164 246 3079
Fragments arranged by size:
3079 988 961 281 246 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KasI G'GCGC_C
Cuts at: 2740 2740
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 4833 5017 1305
Size: 888 2640 184 2007
Fragments arranged by size:
2640 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 10
Size: 54 1425 343 378 3519
Fragments arranged by size:
3519 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 2732 3002 3677 4036 4195 518
Size: 648 1068 498 270 675 359 159 2042
Fragments arranged by size:
2042 1068 675 648 498 359 270 159
MspA1I CmG'CkG
Cuts at: 1606 2526 2550 2577 2631 2982 3135 3812 4960
Size: 920 24 27 54 351 153 677 1148
Cuts at: 4960 5205 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 920 677 351 245 153 54
27 24
NarI GG'CG_CC
Cuts at: 2741 2741
Size: 5719
NcoI C'CATG_G
Cuts at: 513 1065 2022 2097 513
Size: 552 957 75 4135
Fragments arranged by size:
4135 957 552 75
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2425 2477 2500 2707 3077 4622 1132
Size: 97 1196 52 23 207 370 1545 2229
Fragments arranged by size:
2229 1545 1196 370 207 97 52 23
PasI CC'CwG_GG
Cuts at: 1348 2755 2908 2917 1348
Size: 1407 153 9 4150
Fragments arranged by size:
4150 1407 153 9
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PfoI T'CCnGG_A
Cuts at: 2790 2871 2790
Size: 81 5638
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2481 2863 2974 2481
Size: 382 111 5226
Fragments arranged by size:
5226 382 111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2505 2825 3071 830
Size: 1675 320 246 3478
Fragments arranged by size:
3478 1675 320 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2577 2982 2577
Size: 405 5314
SalI G'TCGA_C
Cuts at: 1936 2897 3061 1936
Size: 961 164 4594
Fragments arranged by size:
4594 961 164
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 2465 3967 5679 2465
Size: 1502 1712 2505
Fragments arranged by size:
2505 1712 1502
SexAI A'CCwGG_T
Cuts at: 2319 2319
Size: 5719
SfcI C'TryA_G
Cuts at: 826 1045 2501 2821 3067 4202 4883 5074 826
Size: 219 1456 320 246 1135 681 191 1471
Fragments arranged by size:
1471 1456 1135 681 320 246 219 191
SfoI GGC'GCC
Cuts at: 2742 2742
Size: 5719
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4724 4986 820
Size: 197 926 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3016 3510 493
Size: 2523 494 2702
Fragments arranged by size:
2702 2523 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 2425 2477 3077 1132
Size: 1293 52 600 3774
Fragments arranged by size:
3774 1293 600 52
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2022 2097 2211 2370 3158 513
Size: 552 957 75 114 159 788 3074
Fragments arranged by size:
3074 957 788 552 159 114 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 3746 3763 3916 4101 4546 325
Size: 1255 561 1605 17 153 185 445 1498
Fragments arranged by size:
1605 1498 1255 561 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 2463 2706
Size: 274 80 33 51 1443 294 192 243
Cuts at: 2706 3965 5677 96
Size: 1259 1712 138
Fragments arranged by size:
1712 1443 1259 294 274 243 192 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2377 2734 2752 2203
Size: 174 357 18 5170
Fragments arranged by size:
5170 357 174 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2191 3527 276
Size: 53 83 186 936 657 1336 2468
Fragments arranged by size:
2468 1336 936 657 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI