Restriction map for
pFN21ASDA0263
(Circular) (Six-base) MAPSORT of: pFN21ASDA0263.seq Check: 9609 from: 1 to: 6052
pFN21ASDA0263 6052 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2803 2803
Size: 6052
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3395 1937
Size: 1458 4594
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 4115 5499 5767 1632
Size: 1085 1398 1384 268 1917
Fragments arranged by size:
1917 1398 1384 1085 268
AfeI AGC'GCT
Cuts at: 2192 2192
Size: 6052
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 2727 3288 4951 5928 1203
Size: 1524 561 1663 977 1327
Fragments arranged by size:
1663 1524 1327 977 561
AgeI A'CCGG_T
Cuts at: 2391 4883 2391
Size: 2492 3560
AhdI GACnn_n'nnGTC
Cuts at: 3079 4781 3079
Size: 1702 4350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2156 1184
Size: 972 5080
AlwNI CAG_nnn'CTG
Cuts at: 2596 2802 3795 5367 2596
Size: 206 993 1572 3281
Fragments arranged by size:
3281 1572 993 206
ApaLI G'TGCA_C
Cuts at: 2663 4109 5265 2663
Size: 1446 1156 3450
Fragments arranged by size:
3450 1446 1156
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 3170 3377 1870
Size: 73 238 989 207 4545
Fragments arranged by size:
4545 989 238 207 73
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 4828 618
Size: 325 217 627 1586 1455 1842
Fragments arranged by size:
1842 1586 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 2459 2874 3371 1391
Size: 715 353 415 497 4072
Fragments arranged by size:
4072 715 497 415 353
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5081
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4663 136
Size: 107 122 71 631 605 2991 1525
Fragments arranged by size:
2991 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6047 6047
Size: 6052
BlpI GC'TnA_GC
Cuts at: 2597 3469 2597
Size: 872 5180
Bme1580I G_kGCm'C
Cuts at: 1790 2667 2708 4113 5269 1790
Size: 877 41 1405 1156 2573
Fragments arranged by size:
2573 1405 1156 877 41
BmgBI CAC'GTC
Cuts at: 1318 2936 1318
Size: 1618 4434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3783 4741 442
Size: 416 53 83 399 24 2366 958 1753
Fragments arranged by size:
2366 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2120 2590 3001 3008 3320 4712 1239
Size: 861 20 470 411 7 312 1392 2579
Fragments arranged by size:
2579 1392 861 470 411 312 20 7
Bpu10I CC'TnA_GC
Cuts at: 2957 2957
Size: 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2560 2672 3080 4177 5042 5340 2462
Size: 98 112 408 1097 865 298 3174
Fragments arranged by size:
3174 1097 865 408 298 112 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2076 2438 4715 882
Size: 617 577 362 2277 2219
Fragments arranged by size:
2277 2219 617 577 362
BsaAI yAC'GTr
Cuts at: 493 1204 3289 3349 3843 493
Size: 711 2085 60 494 2702
Fragments arranged by size:
2702 2085 711 494 60
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2793 3128 3859 4241
Size: 53 83 186 936 1260 335 731 382
Cuts at: 4241 4876 275
Size: 635 1451
Fragments arranged by size:
1451 1260 936 731 635 382 335 186
83 53
BsaWI w'CCGG_w
Cuts at: 2391 4485 4883 5157 5304 5715 2391
Size: 2094 398 274 147 411 2728
Fragments arranged by size:
2728 2094 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2563 2593 1178
Size: 30 165 30 1160 30 4637
Fragments arranged by size:
4637 1160 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 3175 1186
Size: 1249 740 4063
Fragments arranged by size:
4063 1249 740
BseYI C'CCAG_C
Cuts at: 2788 3143 3155 5255 5751 2788
Size: 355 12 2100 496 3089
Fragments arranged by size:
3089 2100 496 355 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 2474 2897 1163
Size: 573 636 102 423 4318
Fragments arranged by size:
4318 636 573 423 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2459 2667 2777 3117 4113 4198 5269 2459
Size: 208 110 340 996 85 1071 3242
Fragments arranged by size:
3242 1071 996 340 208 110 85
BsmI GAATG_Cn'
Cuts at: 2071 3563 3656 2071
Size: 1492 93 4467
Fragments arranged by size:
4467 1492 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3098 3098
Size: 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2459 2667 2708 2777 2874 3117
Size: 399 316 353 208 41 69 97 243
Cuts at: 3117 3371 4113 4198 5269 1391
Size: 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 316 254 243
208 97 85 69 41
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2803 2937 3407 844
Size: 1959 134 470 3489
Fragments arranged by size:
3489 1959 470 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2992 3939 1394
Size: 429 1169 947 3507
Fragments arranged by size:
3507 1169 947 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2899 4547 4721 65
Size: 1183 1651 1648 174 1396
Fragments arranged by size:
1651 1648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2391 3078 4696 4883 1700
Size: 110 581 687 1618 187 2869
Fragments arranged by size:
2869 1618 687 581 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3051 794
Size: 2257 3795
BstBI TT'CG_AA
Cuts at: 2007 4856 2007
Size: 2849 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6052
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2047
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 3382 3749 4134 4151 5603 5689 5701 6047
Size: 1410 367 385 17 1452 86 12 346
Cuts at: 6047 1972
Size: 1977
Fragments arranged by size:
1977 1452 1410 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3120 3120
Size: 6052
BtgI C'CryG_G
Cuts at: 513 1065 2040 2866 513
Size: 552 975 826 3699
Fragments arranged by size:
3699 975 826 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2736 487
Size: 683 56 1510 3803
Fragments arranged by size:
3803 1510 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 2413 3568 4362 4382 2407
Size: 6 1155 794 20 4077
Fragments arranged by size:
4077 1155 794 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DraIII CAC_nnn'GTG
Cuts at: 2161 2163 2161
Size: 2 6050
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2090 5059 809
Size: 507 774 2969 1802
Fragments arranged by size:
2969 1802 774 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2863 3499 4388
Size: 54 1425 50 275 18 1033 636 889
Cuts at: 4388 6017 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1033 889 636 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3982 1353
Size: 2629 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2139 4639 5013 5159 387
Size: 1064 138 550 2500 374 146 1280
Fragments arranged by size:
2500 1280 1064 550 374 146 138
EcoICRI GAG'CTC
Cuts at: 2457 2457
Size: 6052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2120 2590 2717 3001 3008 3320
Size: 393 468 20 470 127 284 7 312
Cuts at: 3320 4115 4712 5499 5767 1239
Size: 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 470 468 393 312
284 268 127 20 7
EcoO109I rG'GnC_Cy
Cuts at: 2506 2971 3179 2506
Size: 465 208 5379
Fragments arranged by size:
5379 465 208
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 2194 5199 1678
Size: 516 3005 2531
Fragments arranged by size:
3005 2531 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 3065 3097 3113
Size: 32 135 32 1142 32 319 32 16
Cuts at: 3113 3145 4693 4725 4767 4799 1373
Size: 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 1142 319 135 42 32 32
32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 2727 5928 2727
Size: 3201 2851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 5166 5350 1305
Size: 1365 2496 184 2007
Fragments arranged by size:
2496 2007 1365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2721 2813 4010 4369 4528 518
Size: 648 879 676 92 1197 359 159 2042
Fragments arranged by size:
2042 1197 879 676 648 359 159 92
MspA1I CmG'CkG
Cuts at: 1606 2596 2735 2824 3468 4145 5293 5538 1606
Size: 990 139 89 644 677 1148 245 2120
Fragments arranged by size:
2120 1148 990 677 644 245 139 89
NcoI C'CATG_G
Cuts at: 513 1065 2040 513
Size: 552 975 4525
Fragments arranged by size:
4525 975 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2608 2812 3410 4955 1132
Size: 97 1379 204 598 1545 2229
Fragments arranged by size:
2229 1545 1379 598 204 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6052
PfoI T'CCnGG_A
Cuts at: 2097 3246 2097
Size: 1149 4903
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2506 3179 2506
Size: 673 5379
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PstI C_TGCA'G
Cuts at: 830 2849 3404 830
Size: 2019 555 3478
Fragments arranged by size:
3478 2019 555
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2596 2735 2824 2596
Size: 139 89 5824
Fragments arranged by size:
5824 139 89
SacI G_AGCT'C
Cuts at: 2459 2459
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SanDI GG'GwC_CC
Cuts at: 3179 3179
Size: 6052
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 2858 4300 6012 2858
Size: 1442 1712 2898
Fragments arranged by size:
2898 1712 1442
SfcI C'TryA_G
Cuts at: 826 1045 2845 3400 4535 5216 5407 826
Size: 219 1800 555 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 555 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 2183 3379 1872
Size: 311 1196 4545
Fragments arranged by size:
4545 1196 311
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 2539 2651 3095 4156 5057
Size: 197 926 498 98 112 444 1061 901
Cuts at: 5057 5319 820
Size: 262 1553
Fragments arranged by size:
1553 1061 926 901 498 444 262 197
112 98
SnaBI TAC'GTA
Cuts at: 493 3349 3843 493
Size: 2856 494 2702
Fragments arranged by size:
2856 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 2608 2812 3410 1132
Size: 1476 204 598 3774
Fragments arranged by size:
3774 1476 598 204
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 2999 1517
Size: 1482 4570
StyI C'CwwG_G
Cuts at: 513 1065 2040 2619 3195 3491 513
Size: 552 975 579 576 296 3074
Fragments arranged by size:
3074 975 579 576 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2640 4079 4096 4249 4434 4879 325
Size: 1255 1060 1439 17 153 185 445 1498
Fragments arranged by size:
1498 1439 1255 1060 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 4298 6010
Size: 274 80 33 51 1443 879 1442 1712
Cuts at: 6010 96
Size: 138
Fragments arranged by size:
1712 1443 1442 879 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6012 6044 6012
Size: 32 6020
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2522 2522
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 2181 3377 1870
Size: 311 1196 4545
Fragments arranged by size:
4545 1196 311
XmnI GAAnn'nnTTC
Cuts at: 2225 4181 2225
Size: 1956 4096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI