Restriction map for
pFN21ASDA0262
(Circular) (Six-base) MAPSORT of: pFN21ASDA0262.seq Check: 4686 from: 1 to: 7162
pFN21ASDA0262 7162 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3028 3028
Size: 7162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4972 278
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2967 4505 1937
Size: 1030 1538 4594
Fragments arranged by size:
4594 1538 1030
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 5289 5662 1563
Size: 3726 373 3063
Fragments arranged by size:
3726 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2616 4300 4350 4401 5225 6609 6877 1632
Size: 984 1684 50 51 824 1384 268 1917
Fragments arranged by size:
1917 1684 1384 984 824 268 51 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6965
AflIII A'CryG_T
Cuts at: 1203 6061 7038 1203
Size: 4858 977 1327
Fragments arranged by size:
4858 1327 977
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 5891 5891
Size: 7162
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3966 1184
Size: 2782 4380
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2066
Size: 32 7130
AlwNI CAG_nnn'CTG
Cuts at: 3043 3088 3389 3401 3653 3747 4168 4905 6477
Size: 45 301 12 252 94 421 737 1572
Cuts at: 6477 3043
Size: 3728
Fragments arranged by size:
3728 1572 737 421 301 252 94 45
12
ApaI G_GGCC'C
Cuts at: 4021 4021
Size: 7162
ApaLI G'TGCA_C
Cuts at: 5219 6375 5219
Size: 1156 6006
ApoI r'AATT_y
Cuts at: 1493 2455 4309 4471 4697 4849 6958 1493
Size: 962 1854 162 226 152 2109 1697
Fragments arranged by size:
2109 1854 1697 962 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 5716 160
Size: 872 4684 1606
Fragments arranged by size:
4684 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 3153 3635 3846 3929 4487 1870
Size: 73 1167 43 482 211 83 558 4545
Fragments arranged by size:
4545 1167 558 482 211 83 73 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2625 2658 2625
Size: 33 7129
BamHI G'GATC_C
Cuts at: 2198 4492 4859 2198
Size: 2294 367 4501
Fragments arranged by size:
4501 2294 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4483 5938 618
Size: 325 217 627 2696 1455 1842
Fragments arranged by size:
2696 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 3147 3635 4021 4481 1391
Size: 645 1111 488 386 460 4072
Fragments arranged by size:
4072 1111 645 488 460 386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 2604 2801 4143 928
Size: 971 472 233 197 1342 3947
Fragments arranged by size:
3947 1342 971 472 233 197
BbvCI CC'TCA_GC
Cuts at: 3521 4169 3521
Size: 648 6514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 5353 5387 1903
Size: 34 2486 34 896 34 3678
Fragments arranged by size:
3678 2486 896 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5054 6264 5054
Size: 1210 5952
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7162
BfrBI ATG'CAT
Cuts at: 2766 2766
Size: 7162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5773 136
Size: 107 122 71 631 605 4101 1525
Fragments arranged by size:
4101 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3642 7157 3642
Size: 3515 3647
BlpI GC'TnA_GC
Cuts at: 2030 4579 2030
Size: 2549 4613
Bme1580I G_kGCm'C
Cuts at: 1790 4021 4299 5223 6379 1790
Size: 2231 278 924 1156 2573
Fragments arranged by size:
2573 2231 1156 924 278
BmgBI CAC'GTC
Cuts at: 1318 2799 1318
Size: 1481 5681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2407 2656 4293
Size: 416 53 83 399 24 990 249 1637
Cuts at: 4293 4893 5851 442
Size: 600 958 1753
Fragments arranged by size:
1753 1637 990 958 600 416 399 249
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3817 3661
Size: 156 7006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2257 3080 3305 3644 5822 1239
Size: 937 81 823 225 339 2178 2579
Fragments arranged by size:
2579 2178 937 823 339 225 81
Bpu10I CC'TnA_GC
Cuts at: 3521 4169 3521
Size: 648 6514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3131 5287 6152 6450 2831
Size: 300 2156 865 298 3543
Fragments arranged by size:
3543 2156 865 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3781 5825 882
Size: 617 2282 2044 2219
Fragments arranged by size:
2282 2219 2044 617
BsaAI yAC'GTr
Cuts at: 493 1204 4459 4953 493
Size: 711 3255 494 2702
Fragments arranged by size:
3255 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3447 7156 3447
Size: 3709 3453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4969 5351 5986 275
Size: 53 83 186 936 3436 382 635 1451
Fragments arranged by size:
3436 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5595 5993 6267 6414 6825 5595
Size: 398 274 147 411 5932
Fragments arranged by size:
5932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2078 2096 2108 2794
Size: 30 165 30 663 12 18 12 686
Cuts at: 2794 2824 3278 3308 4407 4437 1178
Size: 30 454 30 1099 30 3903
Fragments arranged by size:
3903 1099 686 663 454 165 30 30
30 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2301 3077 3110 3389 3643 3899 3964
Size: 897 218 776 33 279 254 256 65
Cuts at: 3964 4023 1186
Size: 59 4325
Fragments arranged by size:
4325 897 776 279 256 254 218 65
59 33
BseYI C'CCAG_C
Cuts at: 2514 2757 6365 6861 2514
Size: 243 3608 496 2815
Fragments arranged by size:
3608 2815 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2756 3029 3563 1163
Size: 573 1020 273 534 4762
Fragments arranged by size:
4762 1020 573 534 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4612 5373 6401 1540
Size: 275 186 2611 761 1028 2301
Fragments arranged by size:
2611 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 3147 5223 5308 6379 2036
Size: 1111 2076 85 1071 2819
Fragments arranged by size:
2819 2076 1111 1071 85
BsmI GAATG_Cn'
Cuts at: 4673 4766 4673
Size: 93 7069
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 3147 3635 4021 4299 4481 5223
Size: 399 246 1111 488 386 278 182 742
Cuts at: 5223 5308 6379 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1111 1071 742 488 399 386 278
246 182 85
BspEI T'CCGG_A
Cuts at: 6825 6825
Size: 7162
BspHI T'CATG_A
Cuts at: 4341 5051 6781 4341
Size: 710 1730 4722
Fragments arranged by size:
4722 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3028 3977 4517 844
Size: 2184 949 540 3489
Fragments arranged by size:
3489 2184 949 540
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 5049 1394
Size: 429 313 2913 3507
Fragments arranged by size:
3507 2913 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 5657 5831 65
Size: 1183 4409 174 1396
Fragments arranged by size:
4409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 5806 5993 1700
Size: 110 346 3650 187 2869
Fragments arranged by size:
3650 2869 346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7162
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7162
BssSI C'ACGA_G
Cuts at: 3602 5222 6234 3602
Size: 1620 1012 4530
Fragments arranged by size:
4530 1620 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3043 794
Size: 2249 4913
BstBI TT'CG_AA
Cuts at: 2007 5966 2007
Size: 3959 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7162
BstXI CCAn_nnnn'nTGG
Cuts at: 2420 2420
Size: 7162
BstYI r'GATC_y
Cuts at: 1972 2198 2842 3642 4369 4492 4859 5244 5261
Size: 226 644 800 727 123 367 385 17
Cuts at: 5261 6713 6799 6811 7157 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 800 727 644 385 367 346
226 123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3402 3402
Size: 7162
BtgI C'CryG_G
Cuts at: 513 1065 2473 3315 4012 513
Size: 552 1408 842 697 3663
Fragments arranged by size:
3663 1408 842 697 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2193 487
Size: 683 56 967 5456
Fragments arranged by size:
5456 967 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 3390 4089 4678 5472 5492 2506
Size: 884 699 589 794 20 4176
Fragments arranged by size:
4176 884 794 699 589 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3639 3674 529
Size: 35 3075 35 4017
Fragments arranged by size:
4017 3075 35 35
DraI TTT'AAA
Cuts at: 4466 4466
Size: 7162
DraIII CAC_nnn'GTG
Cuts at: 2789 3586 2789
Size: 797 6365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4081 6169 809
Size: 507 2765 2088 1802
Fragments arranged by size:
2765 2088 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2476 3177 4609
Size: 54 1425 50 275 18 646 701 1432
Cuts at: 4609 5498 7127 8
Size: 889 1629 43
Fragments arranged by size:
1629 1432 1425 889 701 646 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4609 1537
Size: 275 2797 4090
Fragments arranged by size:
4090 2797 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2107 2353 2909 3153 3366 3812 4377 5092
Size: 754 246 556 244 213 446 565 715
Cuts at: 5092 1353
Size: 3423
Fragments arranged by size:
3423 754 715 565 556 446 246 244
213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2086 2128 5749 6123 6269 387
Size: 1064 138 497 42 3621 374 146 1280
Fragments arranged by size:
3621 1280 1064 497 374 146 138 42
EcoICRI GAG'CTC
Cuts at: 2034 3145 2034
Size: 1111 6051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2257 2616 3080 3305 3644 4300
Size: 393 544 81 359 464 225 339 656
Cuts at: 4300 4350 4401 5225 5822 6609 6877 1239
Size: 50 51 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 656 597 544 464 393
359 339 268 225 81 51 50
EcoO109I rG'GnC_Cy
Cuts at: 3992 4017 4018 3992
Size: 25 1 7136
Fragments arranged by size:
7136 25 1
EcoRI G'AATT_C
Cuts at: 4471 4849 4471
Size: 378 6784
EcoRV GAT'ATC
Cuts at: 3710 3710
Size: 7162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2310 2578 3526 2310
Size: 268 948 5946
Fragments arranged by size:
5946 948 268
FspI TGC'GCA
Cuts at: 2669 5668 2669
Size: 2999 4163
FspAI rTGC'GCAy
Cuts at: 2669 2669
Size: 7162
HaeII r_GCGC'y
Cuts at: 1678 3835 6309 1678
Size: 2157 2474 2531
Fragments arranged by size:
2531 2474 2157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 2794 2826 3152
Size: 32 135 32 724 32 466 32 326
Cuts at: 3152 3184 3278 3310 3811 3843 4361 4393 5803
Size: 32 94 32 501 32 518 32 1410
Cuts at: 5803 5835 5877 5909 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1410 724 518 501 466 326 135
94 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2644 4506 4752 669
Size: 988 281 706 1862 246 3079
Fragments arranged by size:
3079 1862 988 706 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7162
HpaI GTT'AAC
Cuts at: 2644 4752 2644
Size: 2108 5054
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 4761 4761
Size: 7162
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2109 4156 6276 6460 1305
Size: 780 24 2047 2120 184 2007
Fragments arranged by size:
2120 2047 2007 780 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2478 10
Size: 54 1425 343 646 4694
Fragments arranged by size:
4694 1425 646 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2877 2900 5120 5479 5638 518
Size: 648 1711 23 2220 359 159 2042
Fragments arranged by size:
2220 2042 1711 648 359 159 23
MspA1I CmG'CkG
Cuts at: 1606 2029 2079 2908 3088 4578 5255 6403 6648
Size: 423 50 829 180 1490 677 1148 245
Cuts at: 6648 1606
Size: 2120
Fragments arranged by size:
2120 1490 1148 829 677 423 245 180
50
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 7162
NcoI C'CATG_G
Cuts at: 513 1065 4012 513
Size: 552 2947 3663
Fragments arranged by size:
3663 2947 552
NdeI CA'TA_TG
Cuts at: 387 3570 387
Size: 3183 3979
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 7162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NsiI A_TGCA'T
Cuts at: 2768 2768
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 2766 4520 6065 1132
Size: 97 1537 1754 1545 2229
Fragments arranged by size:
2229 1754 1545 1537 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7162
PciI A'CATG_T
Cuts at: 6061 6061
Size: 7162
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2587 1108
Size: 1479 5683
PfoI T'CCnGG_A
Cuts at: 3138 3138
Size: 7162
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 5231 5263 6744 6776 2066
Size: 32 3133 32 1481 32 2452
Fragments arranged by size:
3133 2452 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3992 3992
Size: 7162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 2211 4732 2211
Size: 2521 4641
PspOMI G'GGCC_C
Cuts at: 4017 4017
Size: 7162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2550 2582 2550
Size: 32 7130
PstI C_TGCA'G
Cuts at: 830 2725 4514 830
Size: 1895 1789 3478
Fragments arranged by size:
3478 1895 1789
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
PvuII CAG'CTG
Cuts at: 2908 3088 2908
Size: 180 6982
SacI G_AGCT'C
Cuts at: 2036 3147 2036
Size: 1111 6051
SalI G'TCGA_C
Cuts at: 1936 4504 1936
Size: 2568 4594
SanDI GG'GwC_CC
Cuts at: 3992 3992
Size: 7162
SapI GCTCTTCn'nnn_
Cuts at: 2353 2353
Size: 7162
SbfI CC_TGCA'GG
Cuts at: 4514 4514
Size: 7162
ScaI AGT'ACT
Cuts at: 3008 5410 7122 3008
Size: 2402 1712 3048
Fragments arranged by size:
3048 2402 1712
SfcI C'TryA_G
Cuts at: 826 1045 2721 3938 4510 5645 6326 6517 826
Size: 219 1676 1217 572 1135 681 191 1471
Fragments arranged by size:
1676 1471 1217 1135 681 572 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7162
SmaI CCC'GGG
Cuts at: 1872 4489 1872
Size: 2617 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 3146 3929 5266 6167 6429
Size: 197 926 903 300 783 1337 901 262
Cuts at: 6429 820
Size: 1553
Fragments arranged by size:
1553 1337 926 903 901 783 300 262
197
SnaBI TAC'GTA
Cuts at: 493 4459 4953 493
Size: 3966 494 2702
Fragments arranged by size:
3966 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7162
SphI G_CATG'C
Cuts at: 1132 2766 4520 1132
Size: 1634 1754 3774
Fragments arranged by size:
3774 1754 1634
SspI AAT'ATT
Cuts at: 5 52 5086 5
Size: 47 5034 2081
Fragments arranged by size:
5034 2081 47
StuI AGG'CCT
Cuts at: 1517 3419 1517
Size: 1902 5260
StyI C'CwwG_G
Cuts at: 513 1065 3243 4012 4601 513
Size: 552 2178 769 589 3074
Fragments arranged by size:
3074 2178 769 589 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4102 5189 5206 5359 5544 5989 325
Size: 1255 2522 1087 17 153 185 445 1498
Fragments arranged by size:
2522 1498 1255 1087 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3006 5408 7120
Size: 274 80 33 51 1443 1029 2402 1712
Cuts at: 7120 96
Size: 138
Fragments arranged by size:
2402 1712 1443 1029 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7122 7154 7122
Size: 32 7130
Tth111I GACn'n_nGTC
Cuts at: 1316 3699 1316
Size: 2383 4779
XbaI T'CTAG_A
Cuts at: 3344 4498 3344
Size: 1154 6008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3019 3557 3019
Size: 538 6624
XhoI C'TCGA_G
Cuts at: 1943 3929 1943
Size: 1986 5176
XmaI C'CCGG_G
Cuts at: 1870 4487 1870
Size: 2617 4545
XmnI GAAnn'nnTTC
Cuts at: 5291 5291
Size: 7162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4970 276
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I EcoNI
FseI KasI NarI NruI PacI PmlI PspXI RsrII
SacII SexAI SfiI SfoI SrfI SwaI